3株真菌固态发酵产木质素降解酶的研究
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摘要
对菌株JS-1008、米曲霉CGMCC5992、黄孢原毛平革菌CICC40719等3株真菌固态发酵产木质素降解酶、纤维素酶、半纤维素酶进行了研究。结果表明,3株菌株中,米曲霉发酵产木质素降解酶活性最高,木质素过氧化物酶和锰过氧化物酶活性可达2.08、1.79 U/g DS,产纤维素酶、半纤维素酶活性则相对较低,分别为1.69、4.19 U/g DS,木质素降解率为7.23%;菌株JS-1008产木质素降解酶、纤维素酶、半纤维素酶的活性均较低,木质素降解率最低;黄孢原毛平革菌产木质素降解酶的水平最低,木质素过氧化物酶、锰过氧化物酶的活性分别为0.40、0.51 U/g DS,但产纤维素酶、半纤维素酶的活性最高,分别达到2.54、10.86 U/g DS,木质素降解率达11.7%。
引文
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[2]张爱武,董斌,康伟.白腐真菌对秸秆的降解效果及影响因素[J].饲料研究,2011(5):15-17.
[3]张力,邵喜霞,韩大勇.白腐真菌木质素降解酶系研究进展[J].吉林畜牧兽医,2009,30(2):9-12.
[4]Iandolo D,Amore A,Birolo L,et al.Fungal solid state fermentation on agro-industrial wastes for acid wastewater decolorization in a continuous flow packed-bed bioreactor[J].Bioresource Technology,2011,102(16):7603-7607.
[5]杨暖,张妙直,宋洪英.木质素过氧化物酶应用研究进展[J].现代农业科学,2009,16(4):19-20.
[6]吴会广,Cai Y J,苑博华,等.锰过氧化物酶产生菌的发酵优化及酶学性质研究[J].食品与机械,2008,24(4):17-21.
[7]张欢,柴立元,朱咏华,等.3株细菌降解木质素的条件调控研究[J].中南大学学报(自然科学版),2011,42(10):2922-2928.
[8]Bermek H,Yazici H,Ozturk H,et al.Purification and characterization of manganese peroxidase from wood-degrading fungus Trichophyton rubrum LSK-27[J].Enzyme and Microbial Technology,2004,35(1):87-92.
[9]Zouari-Mechichi H,Mechichi T,Dhouib A,et al.Laccase purification and characterization from Trametes trogii isolated in Tunisia:decolorization of textile dyes by the purified enzyme[J].Enzyme and Microbial Technology,2006,39(1):141-148.
[10]Liu L H,Lin Z W,Zheng T,et al.Fermentation optimization and characterization of the laccase from Pleurotus ostreatus strain 10969[J].Enzyme and Microbial Technology,2009,44(6/7):426-433.
[11]丁梦璇,刘炳梅,张国庆,等.绿色糖单孢菌木质素过氧化物酶的分离纯化及鉴定[J].生物技术通报,2011(8):198-202.
[12]贺新生,杨朝惠,赵春花.三种白腐菌对木质纤维素降解规律的初步研究[J].纤维素科学与技术,2013,20(1):33-38.
[13]荚荣,汤必奎,张晓宾,等.藜芦醇和吐温80对白腐菌产木质素降解酶的影响及在偶氮染料脱色中的作用[J].生物工程学报,2004,20(2):302-305.
[14]Ramirez D A,Munoz S V,Atehortua L,et al.Effects of different wavelengths of light on lignin peroxidase production by the whiterot fungi Phanerochaete chrysosporium grown in submerged cultures[J].Bioresource Technology,2010,101(23):9213-9220.
[15]Li N J,Zeng G M,Huang D L,et al.Oxalate production at different initial Pb2+concentrations and the influence of oxalate during solid-state fermentation of straw with Phanerochaete chrysosporium[J].Bioresource Technology,2011,102(17):8137-8142.
[16]Acharya B K,Mohana S,Jog R,et al.Utilization of anaerobically treated distillery spent wash for production of cellulases under solid-state fermentation[J].Journal of Environmental Management,2010,91(10):2019-2027.
[17]Bailey M J,Biely P,Poutanen K.Interlaboratory testing of methods for assay of xylanase activity[J].Journal of Biotechnology,1992,23(3):257-270.
[18]阮久莉,王勐,毛亮,等.白腐菌锰过氧化物酶对2,2',4,4'-四溴联苯醚的降解[J].环境科学与技术,2012,35(1):20-24.