摘要
以锰过氧化物酶活性为评价指标,研究各种因子对白腐真菌生长及产锰过氧化物酶的影响。结果表明:藜芦醇、苯甲醇和吐温80的添加有利于促进白腐真菌的生长及产锰过氧化物酶活性,在浓度分别为150mg·L-1、150mg·L-1、14mg·L-1时对白腐真菌生长及产锰过氧化物酶促进作用最好;Ca2+、Fe2+对白腐真菌生长及产锰过氧化物酶活性的影响是一致的,均表现为"先促进,后抑制"的作用;Cu2+浓度小于0.4mg·L-1时能略微促进白腐真菌的生长及产锰过氧化物酶活性,大于该浓度时具有较明显的抑制作用。
Taking enzyme activity of manganese-dependent peroxidase as the assessment index,the effects of several factors on the characterizations of growth and production of manganese-dependent peroxidase(MnP)by white rot fungi(Phanerochaete chrysosporium)were studied.Results indicated that,veratryl alcohol(150mg·L-1),benzyl alcohol(150mg·L-1)and Tween 80(14mg·L-1)could stimulate the growth of white rot fungi and activity of MnP.Effects on the growth of white rot fungi and activity of MnP were the same with addition of Ca2+and Fe2+,which was promoted at beginning and inhibited later.When Cu2+concentration was lower than0.4mg·L-1,Cu2+cound promote the growth of white rot fungi and activity of MnP,while it could inhibit the growth of white rot fungi and activity of MnP when the concentration was mare than 0.4mg·L-1.
引文
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