白腐真菌产锰过氧化物酶的研究
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摘要
以锰过氧化物酶活性为评价指标,研究各种因子对白腐真菌生长及产锰过氧化物酶的影响。结果表明:藜芦醇、苯甲醇和吐温80的添加有利于促进白腐真菌的生长及产锰过氧化物酶活性,在浓度分别为150mg·L-1、150mg·L-1、14mg·L-1时对白腐真菌生长及产锰过氧化物酶促进作用最好;Ca2+、Fe2+对白腐真菌生长及产锰过氧化物酶活性的影响是一致的,均表现为"先促进,后抑制"的作用;Cu2+浓度小于0.4mg·L-1时能略微促进白腐真菌的生长及产锰过氧化物酶活性,大于该浓度时具有较明显的抑制作用。
Taking enzyme activity of manganese-dependent peroxidase as the assessment index,the effects of several factors on the characterizations of growth and production of manganese-dependent peroxidase(MnP)by white rot fungi(Phanerochaete chrysosporium)were studied.Results indicated that,veratryl alcohol(150mg·L-1),benzyl alcohol(150mg·L-1)and Tween 80(14mg·L-1)could stimulate the growth of white rot fungi and activity of MnP.Effects on the growth of white rot fungi and activity of MnP were the same with addition of Ca2+and Fe2+,which was promoted at beginning and inhibited later.When Cu2+concentration was lower than0.4mg·L-1,Cu2+cound promote the growth of white rot fungi and activity of MnP,while it could inhibit the growth of white rot fungi and activity of MnP when the concentration was mare than 0.4mg·L-1.
Taking enzyme activity of manganese-dependent peroxidase as the assessment index,the effects of several factors on the characterizations of growth and production of manganese-dependent peroxidase(MnP)by white rot fungi(Phanerochaete chrysosporium)were studied.Results indicated that,veratryl alcohol(150mg·L-1),benzyl alcohol(150mg·L-1)and Tween 80(14mg·L-1)could stimulate the growth of white rot fungi and activity of MnP.Effects on the growth of white rot fungi and activity of MnP were the same with addition of Ca2+and Fe2+,which was promoted at beginning and inhibited later.When Cu2+concentration was lower than0.4mg·L-1,Cu2+cound promote the growth of white rot fungi and activity of MnP,while it could inhibit the growth of white rot fungi and activity of MnP when the concentration was mare than 0.4mg·L-1.
引文
[1]张力,邵喜霞,韩大勇.白腐真菌木质素降解酶系研究进展[J].畜牧与饲料科学,2009,30(1):35-37.
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[7]陈敏,郭倩,姚善泾.响应面法优化白腐菌Pleurotus eryngii-Co007产木质素降解酶条件[J].核农学报,2011,25(3):482-487.
[8]MOREIRA M T,SANROMAN A,FEIJOO G,et al.Control of pellet morphology of filamentous fungi in fluidized bed bioreactors by means of a pulsing flow[J].Enzyme and Microbial Technology,1996,19(4):261-266.
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[10]CANECL A M,ORTH A B,TIEN M.Lignin and veratryl alcohol are not inducers of the ligninolytic system of phanaerochaete chrysosporium[J].Applied and Environment Microbiology,1993,59:2909-2913.
[11]BOCKLE B,MARTINEZ M J,GUILLEN F,et al.Mechanism of peroxidase in activation in liquid cultures of the ligninolytic fun-gus pleurotus pulmonarius[J].Appl Environ Microbiol,1999,65(3):923-928.
[12]JEFERIES T W,CHOI S,KIRK T K.Nutritional regulation of lignin degradation by Phanerochaete chrysosporium[J].Appl Environ Microbiol,1981,42(2):290-296.
[13]韩增辉.表面活性剂构建体系中漆酶对木素模式化合物的催化降解[D].长沙:湖南大学,2012.
[14]李德莹,龚大春,田毅红,等.金属离子对纤维素酶活力影响的研究[J].酿酒科技,2009,18(6):40-42.
[2]PASTI M B,PASZCZYNSKI A,GROZCZYNSKI S.Influence of aromatic substitution patterns on azo dye degradability by Streptomyces spp.and Phanerochaete chrysosporium[J].Appl Environ Microbiol,1992,58(11):3605-3613.
[3]徐海娟,梁文芷.白腐菌降解木素酶系及其作用机理[J].环境污染治理与设备,2000,1(3):51-54.
[4]崔艳红,韩庆功,常魁珍,等.木质层孔菌产锰过氧化物酶条件的优化及酶学性质研究[J].饲料工业,2012,33(12):55-59.
[5]李慧蓉,陈建海.黄孢原毛平革菌对蒽的降解研究[J].化工环保,2000,20(2):11-14.
[6]谭丽泉,余梅,黄敏,等.黄孢原毛平革菌的生长及降解石油条件的优化[J].化工环保,2012,32(1):11-15.
[7]陈敏,郭倩,姚善泾.响应面法优化白腐菌Pleurotus eryngii-Co007产木质素降解酶条件[J].核农学报,2011,25(3):482-487.
[8]MOREIRA M T,SANROMAN A,FEIJOO G,et al.Control of pellet morphology of filamentous fungi in fluidized bed bioreactors by means of a pulsing flow[J].Enzyme and Microbial Technology,1996,19(4):261-266.
[9]PASZCZYNSKI A,CRAWFORD R L,HUYNH V B.Manganese peroxidase of Phanerochaete chrysosporium:Purification[J].Purification Methods in Enzymology,1988,161(2):264-270.
[10]CANECL A M,ORTH A B,TIEN M.Lignin and veratryl alcohol are not inducers of the ligninolytic system of phanaerochaete chrysosporium[J].Applied and Environment Microbiology,1993,59:2909-2913.
[11]BOCKLE B,MARTINEZ M J,GUILLEN F,et al.Mechanism of peroxidase in activation in liquid cultures of the ligninolytic fun-gus pleurotus pulmonarius[J].Appl Environ Microbiol,1999,65(3):923-928.
[12]JEFERIES T W,CHOI S,KIRK T K.Nutritional regulation of lignin degradation by Phanerochaete chrysosporium[J].Appl Environ Microbiol,1981,42(2):290-296.
[13]韩增辉.表面活性剂构建体系中漆酶对木素模式化合物的催化降解[D].长沙:湖南大学,2012.
[14]李德莹,龚大春,田毅红,等.金属离子对纤维素酶活力影响的研究[J].酿酒科技,2009,18(6):40-42.