绿色木霉、红芝、酵母三菌混合发酵稻草秸秆生产蛋白饲料条件的研究
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摘要
试验首先研究了绿色木霉、红芝共同发酵对秸秆中的纤维素、半纤维素、木质素降解的影响,然后进行了二次发酵接种酵母生产蛋白饲料条件的研究.结果表明:绿色木霉、红芝接种比例为1∶4、接种量为35%、共同发酵时间8d、二次发酵时,酵母接种量7%、发酵时间为24h时培养基的真蛋白含量最高.
The aim of the study was to optimize the conditions of fermentation by common fermentation of Trichoderma viride,ganoderma and yeast.A combined fermentation was conducted firstly with T.viride and ganoderma to degrade cellulose,hemicellulose and lignose of straw.Then,the conditions of protein feed production was studied by inoculating yeast with secondary fermentation.When the inoculation proportion of T.viride and ganoderma was 1∶4,inoculation 35%,fermentation 192 h,the inoculation of yeast 7%,fermentation 24 h.Under this condition,the content of real protein in culture medium reached the highest.
The aim of the study was to optimize the conditions of fermentation by common fermentation of Trichoderma viride,ganoderma and yeast.A combined fermentation was conducted firstly with T.viride and ganoderma to degrade cellulose,hemicellulose and lignose of straw.Then,the conditions of protein feed production was studied by inoculating yeast with secondary fermentation.When the inoculation proportion of T.viride and ganoderma was 1∶4,inoculation 35%,fermentation 192 h,the inoculation of yeast 7%,fermentation 24 h.Under this condition,the content of real protein in culture medium reached the highest.
引文
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[16]何东东.产朊假丝酵母的发酵及功能性质研究[D].天津:天津商业大学,2010
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[2]李彩霞,李鹏,王晓琴,等.微生态高蛋白玉米秸秆饲料开发研究[J].甘肃农业科技,2000(5):39-42
[3]Babitskaya V G.Fungi the Producers of Physiologically Active Can pounds in Lignocellulose Biology and Cultivntion[D].Doctoral Sc(Boil)Dissertation,Minsk,Intmikrobiologii AN BSSR,1991,372-416
[4]Stepanova E V,Koroleva O V.Fungal decom position of oat straw during liquid and solid-state fermentation[J].Applied Biochemistry and Microbiology,2003,39(1):65-74
[5]韦丽敏.降解秸秆的白腐真菌的筛选、优化及混菌发酵研究[D].兰州:甘肃农业大学,2013
[6]何桂霞.绿色木霉的筛选及秸秆的二次组合发酵[D].兰州:甘肃农业大学,2013
[7]Y.P.Lu,B.Yang,D.Gregg,J.N.Saddler,S.D.Mansfield.Cellulase adsorption and an evalution of enzyme recycle during hydrolysis of steam exploded softwood residues[J].Applied Biochemistry and Biotechnology,2002,98:641-654
[8]李亚蕾,杨波,罗瑞明.以绿色木霉TrichodermavirideNUA-051及产朊假丝酵母Candidautilis发酵酸解玉米秸秆生产单细胞蛋白的工艺研究[J].食品科技,2006(7):261-264
[9]廖雪义,冯桦林,吴传兵,等.木霉与酵母混合发酵秸秆生产蛋白饲料的研究[J].粮食与饲料工业,2010(10):44-46
[10]陶蕾,王曙阳,曹广益,等.复合菌剂发酵秸秆的作用及应用[J].甘肃农业大学学报,2011,46(),37-40
[11]刘忠元.热带假丝酵母和黑曲霉发酵玉米秸秆条件优化[D].长春:吉林大学,2007
[12]潘峰,史晓丽,杨树林,等.多菌种混合发酵稻草生产蛋白饲料的研究[J].粮食与饲料工业,2001(8):25-26
[13]Bhalla T C,Joshi M.Protein enrichment of apple by co-culture of cellulolytic moulds and yeasts[J].World Journal of Microbiology&Biotechnology,1994(10):116-117
[14]KE L X,WU Q,ZHANG D Q.Bioconversion of rape into a nutritionally enriched substrate by Ganoderma Lucidum and yeas[J].African Journal of Biotechnology,2011,10(29):5648-5653
[15]邵喜霞,张力,韩大勇,等.新型秸秆分解菌的筛选和酶活性研究[J].中兽医医药杂志,2008(3):15-17
[16]何东东.产朊假丝酵母的发酵及功能性质研究[D].天津:天津商业大学,2010
[17]张丽英.饲料分析及饲料质量检测技术[M].北京:中国农业大学出版社,2002:49-63