柴枳夏及汤对胆汁反流性胃炎大鼠胃黏膜的保护作用及机制研究
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摘要
目的探讨柴枳夏及汤对实验性胆汁反流性胃炎(BRG)大鼠胃黏膜的保护作用及机制。方法将Wistar大鼠按照体质量及性别以随机数字表法分为5组:对照组、模型组、铝碳酸镁片(阳性药)组和柴枳夏及汤低、高剂量(生药量12.77、25.54 g/kg)组,除对照组外,ig自制反流液制备大鼠BRG模型。观察对照组及模型组大鼠进食、大小便、毛色、体质量等;肉眼及HE染色后于光镜下观察胃窦黏膜病理组织学变化;ELISA法检测血清GAS及胃窦黏膜PGE2水平。结果与对照组比较,模型组大鼠出现排稀糊便、便中含红黄色黏液、反应迟钝、体质量显著下降(P<0.05)等特点;肉眼可见胃窦部点片状糜烂,有黄绿色胆汁及较多黄色黏液;胃窦黏膜出现大量炎性细胞浸润和肠上皮化生,炎症及肠上皮化生积分显著提高(P<0.01);GAS及PGE2水平明显下降(P<0.01);与模型组比较,柴枳夏及汤高剂量明显改善胃黏膜病理组织损伤形态;减轻炎性细胞浸润和肠上皮化生,显著降低积分(P<0.05、0.01);显著提高GAS及PGE2水平(P<0.01)。结论柴枳夏及汤具有明显的保护胃黏膜的作用,其机制可能与调节血清GAS、胃窦黏膜PGE2水平有关。
Objective To investigate the therapeutic effect and mechanism of Chaizhixiaji Decoction on experimental bile reflux gastritis(BRG) rats. Methods Wistar rats were randomly divided into five groups by random according to weight and sex: control group, model group, Chaizhixiaji Decoction low and high dose groups, and Hydrotalcite Tablets group. Rats were given self-made reflux liquid orally to induce experimental bile reflux gastritis. The diet, stool and urine, hair color and body weight of rats in control group and model group were observed. The histopathological changes of gastric antrum mucosa were observed by naked eyeand light microscope after HE staining. ELISA method was used for detection of serum GAS, PGE2 content in gastric antrum mucosa. Result Compared with control group, rats in model group show thin boicing stool containing red yellow mucus, slow reaction, and body weight decreased significantly(P < 0.05). There were patchy erosions of the gastric antrum with yellow green bile and more yellow mucus of model group in the naked eye. Model control group had a significant rise of inflammatory cells infiltration and intestinal metaplasia, and scores of inflammation and intestinal metaplasia increased significantly(P < 0.01). GAS and PGE2 contents obviously decreased compared with that in control group(P < 0.01). Compare with model group, Chaizhixiaji Decoction of high dosesignificantly improved gastric mucosal tissue damage morphology; reduced the infiltration of inflammatory cells and intestinal metaplasia, which score decreased significantly(P < 0.05, 0.01); and increasedlevels of GAS and PGE2 significantly(P < 0.01). Conclusion Chaizhixiaji Decoction has obvious protective effect on gastric mucosa, and its mechanism may be related with the regulation of GAS, PGE2 contents.
Objective To investigate the therapeutic effect and mechanism of Chaizhixiaji Decoction on experimental bile reflux gastritis(BRG) rats. Methods Wistar rats were randomly divided into five groups by random according to weight and sex: control group, model group, Chaizhixiaji Decoction low and high dose groups, and Hydrotalcite Tablets group. Rats were given self-made reflux liquid orally to induce experimental bile reflux gastritis. The diet, stool and urine, hair color and body weight of rats in control group and model group were observed. The histopathological changes of gastric antrum mucosa were observed by naked eyeand light microscope after HE staining. ELISA method was used for detection of serum GAS, PGE2 content in gastric antrum mucosa. Result Compared with control group, rats in model group show thin boicing stool containing red yellow mucus, slow reaction, and body weight decreased significantly(P < 0.05). There were patchy erosions of the gastric antrum with yellow green bile and more yellow mucus of model group in the naked eye. Model control group had a significant rise of inflammatory cells infiltration and intestinal metaplasia, and scores of inflammation and intestinal metaplasia increased significantly(P < 0.01). GAS and PGE2 contents obviously decreased compared with that in control group(P < 0.01). Compare with model group, Chaizhixiaji Decoction of high dosesignificantly improved gastric mucosal tissue damage morphology; reduced the infiltration of inflammatory cells and intestinal metaplasia, which score decreased significantly(P < 0.05, 0.01); and increasedlevels of GAS and PGE2 significantly(P < 0.01). Conclusion Chaizhixiaji Decoction has obvious protective effect on gastric mucosa, and its mechanism may be related with the regulation of GAS, PGE2 contents.
引文
[1]Dai F,Gong J,Zhang R,et al.Assessment of duodenogastric reflux by combined continuous intragastric PH and bilirubin monitoring[J].World J Gastroenterol,2002,8(2):382-384.
[2]张亚历.实用消化病学:图解诊断与治疗方法[M].清华大学出版社,2009:120-123.
[3]乔善鑫,白玉昊,孙莉.胆汁反流性胃炎的中西诊疗进展[J].中医临床研究,2016,8(13):146-148.
[4]蒋宝平,田磊,张雯,等.胆胃双和汤对胆汁反流性胃炎大鼠的防治作用及对胃肠激素的影响[J].中药新药与临床药理,2014,25(4):450-454.
[5]徐叔云.药理实验方法学[M].北京:人民卫生出版社,2002.
[6]中国中西医结合学会消化系统疾病专业委员会.慢性胃炎的中西医结合诊治方案(草案)[J].中国中西医结合杂志,2005,25(2):172-175.
[7]李晓勇,唐雪生.瑞巴派特与硫糖铝对慢性非萎缩性胃炎的临床疗效及胃黏膜保护作用对比研究[J].医学综述,2014,20(22):4203-4205.
[8]Chan D C,Fan Y M,Lin C K,et al.Roux-en-Y reconstruction after distal gastrectomy to reduce enterogastric reflux and Helicobacter pylori infection[J].J Gastrointest Surg,2007,11(12):1732-1740.
[9]张瑞星,姚树坤,范海燕,等.十二指肠-胃反流物膜的影响[J].诊断学理论与实践,2006,5(1):43.
[10]谢晶日,葛阳,张杨.欣胃颗粒对慢性萎缩性胃炎大鼠血清胃泌素的影响[J].中医药学报,2013,41(3):88-89.麻春杰,王艳,宋美丹,等.胃和冲剂Ⅱ号对慢性萎缩性胃炎大鼠胃蛋白酶活性、血清GAS和PGE2的影响[J].中国中医药科技,2011,18(1):17-18.
[11]姚萍,高鸿亮,刘发.荜茇根对胆汁反流胃炎大鼠模型的防治作用及对胃泌素?PGE2?TNF-α?IL-8含量的影响[J].中药药理与临床,2012,28(1):111-114.
[12]Comstock D.Dealing with postcholecystectomy syndrome[J].Nuersing,2008,38(4):17-19.
[13]赵福兰.原发性胆汁反流性胃炎的诊治研究进展[J].新疆医学,2012,42(5):61-65.
[14]Abe H,Murakami K,Satoh S,et al.Influence of bile reflux and Helicobacter pylori infection on gastritis in the remnant gastric mucosa after distal gastrectomy[J].J Gastroenterol,2005,40(6):563-569.
[2]张亚历.实用消化病学:图解诊断与治疗方法[M].清华大学出版社,2009:120-123.
[3]乔善鑫,白玉昊,孙莉.胆汁反流性胃炎的中西诊疗进展[J].中医临床研究,2016,8(13):146-148.
[4]蒋宝平,田磊,张雯,等.胆胃双和汤对胆汁反流性胃炎大鼠的防治作用及对胃肠激素的影响[J].中药新药与临床药理,2014,25(4):450-454.
[5]徐叔云.药理实验方法学[M].北京:人民卫生出版社,2002.
[6]中国中西医结合学会消化系统疾病专业委员会.慢性胃炎的中西医结合诊治方案(草案)[J].中国中西医结合杂志,2005,25(2):172-175.
[7]李晓勇,唐雪生.瑞巴派特与硫糖铝对慢性非萎缩性胃炎的临床疗效及胃黏膜保护作用对比研究[J].医学综述,2014,20(22):4203-4205.
[8]Chan D C,Fan Y M,Lin C K,et al.Roux-en-Y reconstruction after distal gastrectomy to reduce enterogastric reflux and Helicobacter pylori infection[J].J Gastrointest Surg,2007,11(12):1732-1740.
[9]张瑞星,姚树坤,范海燕,等.十二指肠-胃反流物膜的影响[J].诊断学理论与实践,2006,5(1):43.
[10]谢晶日,葛阳,张杨.欣胃颗粒对慢性萎缩性胃炎大鼠血清胃泌素的影响[J].中医药学报,2013,41(3):88-89.麻春杰,王艳,宋美丹,等.胃和冲剂Ⅱ号对慢性萎缩性胃炎大鼠胃蛋白酶活性、血清GAS和PGE2的影响[J].中国中医药科技,2011,18(1):17-18.
[11]姚萍,高鸿亮,刘发.荜茇根对胆汁反流胃炎大鼠模型的防治作用及对胃泌素?PGE2?TNF-α?IL-8含量的影响[J].中药药理与临床,2012,28(1):111-114.
[12]Comstock D.Dealing with postcholecystectomy syndrome[J].Nuersing,2008,38(4):17-19.
[13]赵福兰.原发性胆汁反流性胃炎的诊治研究进展[J].新疆医学,2012,42(5):61-65.
[14]Abe H,Murakami K,Satoh S,et al.Influence of bile reflux and Helicobacter pylori infection on gastritis in the remnant gastric mucosa after distal gastrectomy[J].J Gastroenterol,2005,40(6):563-569.