好氧颗粒污泥的驯化及其对含四环素模拟废水的除污染效能
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摘要
在序批式反应器(SBR)中驯化好氧颗粒污泥(AGS),研究不同剪切力对其形态及性能的影响。在剪切力为1. 86 cm·s~(-1)时,可驯化出成熟且形貌好的AGS,其生物量和沉降性能最好,MLSS和SVI值分别达到6. 0 g·L~(-1)和35 m L·g~(-1)。在优化选定的剪切力条件下,考察驯化好的AGS处理含四环素的模拟畜禽养殖废水的除污染效果,结果表明,驯化后的颗粒污泥对四环素的去除效果明显,去除率从运行初期的74%左右逐渐上升,在第35 d达到稳定,约为90%,而对COD、NH_4~+-N和TP等常规污染指标的去除率分别为98. 56%、86. 14%和93. 58%,与不含四环素废水的对照处理效果相当。采用16S rDNA宏基因组高通量测序技术,对比分析不同运行阶段颗粒污泥样品中微生物的群落结构,结果显示,四环素的去除与AGS中的优势菌种密不可分,包括Comamonas、Flavobacterium、Lactococcus、Shinella、Actinotignum等可能利用四环素作为碳源的优势菌属。
Aerobic granular sludge( AGS) was domesticated in a sequence batch reactor( SBR),and the effects of different shear forces on morphology and properties of granular sludge were studied. The results showed that when the shear force was 1. 86 cm·s~(-1),the mature AGS with the good morphology could be domesticated. Comparative analysis showed that the biomass and sedimentation performance ofthe AGS were the best,and the MLSS and SVI values reached 6. 0 g·L~(-1) and 35 m L·g-1 respectively.Under the optimal shear force condition of 1. 86 cm·s~(-1),the simulated livestock and poultry wastewater containing tetracycline were treated with aerobic granular sludge sequencing batch reactor( AGSBR).The removal efficiency of tetracycline by the acclimated AGS was obvious,the removal rate was about74% at the beginning of operation,then increasing gradually and could eventually maintain at about 90%after 35 days. The removal rates of conventional pollution index such as COD,NH+4-N and TP were98. 56%,86. 14% and 93. 58%,respectively,almost the same as the control experiment. 16 S r DNA macro genome high throughput sequencing technology was used to analyze the microbial community structure in different AGS samples. The results showed that the removal of tetracycline in AGSBR was closely related to the dominant bacteria in AGS,including possible tetracycline degradation bacteria such as Comamonas,Flavobacterium,Lactococcus,Shinella and Actinotignum.
Aerobic granular sludge( AGS) was domesticated in a sequence batch reactor( SBR),and the effects of different shear forces on morphology and properties of granular sludge were studied. The results showed that when the shear force was 1. 86 cm·s~(-1),the mature AGS with the good morphology could be domesticated. Comparative analysis showed that the biomass and sedimentation performance ofthe AGS were the best,and the MLSS and SVI values reached 6. 0 g·L~(-1) and 35 m L·g-1 respectively.Under the optimal shear force condition of 1. 86 cm·s~(-1),the simulated livestock and poultry wastewater containing tetracycline were treated with aerobic granular sludge sequencing batch reactor( AGSBR).The removal efficiency of tetracycline by the acclimated AGS was obvious,the removal rate was about74% at the beginning of operation,then increasing gradually and could eventually maintain at about 90%after 35 days. The removal rates of conventional pollution index such as COD,NH+4-N and TP were98. 56%,86. 14% and 93. 58%,respectively,almost the same as the control experiment. 16 S r DNA macro genome high throughput sequencing technology was used to analyze the microbial community structure in different AGS samples. The results showed that the removal of tetracycline in AGSBR was closely related to the dominant bacteria in AGS,including possible tetracycline degradation bacteria such as Comamonas,Flavobacterium,Lactococcus,Shinella and Actinotignum.
引文
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[24]军李,周延年,何梅.城市污水处理厂好氧颗粒污泥的特性[J].应用与环境生物学报,2008,14(5):640-643.
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[26]BEILIN H,KE L. Adsorption characteristics of tetracycline by anaerobic granular sludge[J]. Journal of Jiangsu Agricultural Sciences,2013,41(3):345-347.
[27]XIN X,LU H,LI Y,et al. Rapid Formation of aerobic granular sludge and its mechanism in a continuous-flow bioreactor[J]. Appllied Biochemistry and Biotechnology,2017,181:424-433.
[28]JINGFENG W,XUAN W,MIN J,et al. Study on sludge characteristics of aerobic granular sludge membrane bioreactor[J]. Environmental Science,2007,28(5):1033-1038.
[29]AIPING L,ZHONGYIN C,KAIMING L,et al. Culture of aerobic granular sludge in mbr and its effect on membrane fouling[J]. China Water&Wastewater,2010,26(19):1-4.
[30]MARAKI S,EVANGELOU G,STAFYLAKI D,et al. Actinotignum schaalii subcutaneous abscesses in a patient with hidradenitis suppurativa:case report and literature review[J]. Anaerobe,2017,43:43-46.
[31]YICHEN L,JUAN C,GUOQING G,et al. Isolation and Characterization of Naphthalene-Degrading Bacteria Comamoca ssp. N19-3 and Detection of Its Naphthalene-Dioxygenase Gene[J]. Research of Environmental Sciences,2008,21(5):27-31.
[32]RODRIGUEZ-SANCHEZ A,MARGARETO A,ROBLEDO-MAHON T,et al. Performance and bacterial community structure of a granular autotrophic nitrogen removal bioreactor amended with high antibiotic concentrations[J]. Chemical Engineering Journal,2017,325:257-269.
[33]BELLAIS S,POIREL L,LEOTARD S,et al. Genetic diversity of carbapenem-hydrolyzing metallo-beta-lactamases from Chryseobacterium(Flavobacterium)indologenes[J]. Antimicrobial Agents and Chemotherapy,2000,44(11):3028-3034.
[34]BELLAIS S,AUBERT D,NAAS T,et al. Molecular and biochemical heterogeneity of class B carbapenem-hydrolyzing beta-Lactamases in chryseobacterium meningosepticum[J]. Antimicrobial Agents and Chemotherapy,2000,44(7):1878-1886.
[2]万莉,邹义龙,弓晓峰,等.电增强零价铁强化厌氧氨氧化处理高氮养猪废水[J].环境科学研究,2015,28(8):1302-1310.
[3]HAN J,WU R,JING H,et al. Review and forecast on biology treatment technology of intensive livestock wastewater[J]. Technology of Water Treatment,2008,34(7):7-11.
[4]WILSON B,PYATT F B. Heavy metal bioaccumulation by the important food plant,Olea europaea L,in an ancient metalliferous polluted area of Cyprus[J]. Bulletin of Environmental Contamination and Toxicology,2007,78(5):390-394.
[5]ZHOU Q,LUO Y,WANG M. Environmental residues and ecotoxicity of antibiotics and their resistance gene pollution:a review[J]. Asian Journal of Ecotoxicology,2007,2(3):243-251.
[6]BOXALL A B,FOGG L A,BLACKWELL P A,et al. Veterinary medicines in the environment[J]. Reviews of Environment Contamination and Toxicology,2004,180:1-91.
[7]POMATI F,NETTING A,CALAMARI D,et al. Effects of erythromycin,tetracycline and ibuprofen on the growth of Synechocystis sp. and Lemna minor[J]. Aquatic Toxicology,2004,67(4):387-396.
[8]BRUIN D L,KREUK D M,VAN H,et al. Aerobic granular sludge technology:an alternative to activated sludge?[J]. Water Science and Technology,2004,49(11-12):1-7.
[9]ZHU J,WILDERER P A. Effect of extended idle conditions on structure and activity of granular activated sludge[J]. Water Research,2003,37(7):2013-2018.
[10]GAO D,YUAN X,LIANG H,et al. Comparison of biological removal via nitrite with real-time control using aerobic granular sludge and flocculent activated sludge[J]. Applied Microbiology&Biotechnology,2011,89(5):1645-1652.
[11]MORALES N,FIGUEROA M,FRA-VZQUEZ A,et al. Operation of an aerobic granular pilot scale SBR plant to treat swine slurry[J]. Process Biochemistry,2013,48:1216-1221.
[12]ZHAO Y,HUANG J,ZHAO H,et al. Microbial community and N removal of aerobic granular sludge at high COD and N loading rates[J]. Bioresource Technology,2013,143:439-446.
[13]TAY J H,LIU Q S,LIU Y. The effects of shear force on the formation,structure and metabolism of aerobic granules[J]. Applied Microbiology and Biotechnology,2001,57(1-2):227-233.
[14]TAY J,YANG S,LIU Y. Hydraulic selection pressure-induced nitrifying granulation in sequencing batch reactors[J]. Applied Microbiology and Biotechnology,2002,59(3):332-337.
[15]国家环境保护总局,水和废水监测分析方法编委会.水和废水监测分析方法[M].第四版.北京:中国环境科学出版社,2002:96-220.
[16]赵霞.好氧颗粒污泥系统处理含PPCPs污水的效能及微生物群落演替[D].哈尔滨:哈尔滨工业大学,2015:32-35.
[17]FROLUND B. Extraction of extracellular polymers from activated sludge using a cation exchange resin[J]. Water Research,1996,30(8):1749-1758.
[18]WANG F,YANG F L,LIU Y H,et al. Cultivation of aerobic granules for simultaneous nitrification and denitrification by seeding different inoculated sludge[J]. Journal of Environmental Science,2005,17(2):268-270.
[19]张小玲,王芳,刘珊.剪切力对好氧颗粒污泥的影响及其脱氮除磷特性研究[J].安全与环境学报,2011,11(4):56-60.
[20]罗小娟.不同水力条件对CANON颗粒污泥微生物胞外聚合物的影响[J].环境科技,2017,30(2):17-22.
[21]DEVLIN T R,OLESZKIEWICZ J A. Cultivation of aerobic granular sludge in continuous flow under various selective pressure[J]. Bioresource Technology,2018,253:281-287.
[22]ADAV S S,LEE D J,LAI J Y. Effects of aeration intensity on formation of phenol-fed aerobic granules and extracellular polymeric substances[J].Applied Microbiology and Biotechnology,2007,77(1):175-182.
[23]LIU Y,YANG S,LIU Q,et al. The role of cell hydrophobicity in the formation of aerobic granules[J]. Current Microbiology,2003,46(4):270-274.
[24]军李,周延年,何梅.城市污水处理厂好氧颗粒污泥的特性[J].应用与环境生物学报,2008,14(5):640-643.
[25]XU X R,LI X Y. Sorption and desorption of antibiotic tetracycline on marine sediments[J]. Chemosphere,2010,78(4):430-436.
[26]BEILIN H,KE L. Adsorption characteristics of tetracycline by anaerobic granular sludge[J]. Journal of Jiangsu Agricultural Sciences,2013,41(3):345-347.
[27]XIN X,LU H,LI Y,et al. Rapid Formation of aerobic granular sludge and its mechanism in a continuous-flow bioreactor[J]. Appllied Biochemistry and Biotechnology,2017,181:424-433.
[28]JINGFENG W,XUAN W,MIN J,et al. Study on sludge characteristics of aerobic granular sludge membrane bioreactor[J]. Environmental Science,2007,28(5):1033-1038.
[29]AIPING L,ZHONGYIN C,KAIMING L,et al. Culture of aerobic granular sludge in mbr and its effect on membrane fouling[J]. China Water&Wastewater,2010,26(19):1-4.
[30]MARAKI S,EVANGELOU G,STAFYLAKI D,et al. Actinotignum schaalii subcutaneous abscesses in a patient with hidradenitis suppurativa:case report and literature review[J]. Anaerobe,2017,43:43-46.
[31]YICHEN L,JUAN C,GUOQING G,et al. Isolation and Characterization of Naphthalene-Degrading Bacteria Comamoca ssp. N19-3 and Detection of Its Naphthalene-Dioxygenase Gene[J]. Research of Environmental Sciences,2008,21(5):27-31.
[32]RODRIGUEZ-SANCHEZ A,MARGARETO A,ROBLEDO-MAHON T,et al. Performance and bacterial community structure of a granular autotrophic nitrogen removal bioreactor amended with high antibiotic concentrations[J]. Chemical Engineering Journal,2017,325:257-269.
[33]BELLAIS S,POIREL L,LEOTARD S,et al. Genetic diversity of carbapenem-hydrolyzing metallo-beta-lactamases from Chryseobacterium(Flavobacterium)indologenes[J]. Antimicrobial Agents and Chemotherapy,2000,44(11):3028-3034.
[34]BELLAIS S,AUBERT D,NAAS T,et al. Molecular and biochemical heterogeneity of class B carbapenem-hydrolyzing beta-Lactamases in chryseobacterium meningosepticum[J]. Antimicrobial Agents and Chemotherapy,2000,44(7):1878-1886.