生长素对川牛膝侧根发育及基因IAA14表达的影响
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摘要
目的:探索生长素IAA对川牛膝Cyathula officinalis Kuan侧根发育和基因IAA14表达的影响。方法:以道地产区乐山金口河3年生川牛膝的种子为材料,在含有不同浓度IAA的MS培养基中培育得到川牛膝幼苗,对其进行根显微结构分析、侧根参数和基因IAA14表达量的考察。结果:川牛膝侧根的起始部分同大多数双子叶植物一样,侧根原基起始于正对木质部的中柱鞘细胞;IAA在一定范围内对川牛膝侧根的发生具有一定的促进作用,且同时下调基因IAA14表达量;平均侧根密度最大值出现在1.83×10~(-5)mg/mL的IAA施加浓度,此时基因IAA14表达量也最低。结论:川牛膝侧根起始于正对木质部的中柱鞘细胞,IAA通过实时调节侧根发育相关基因IAA14在一定范围内促进川牛膝侧根的发育。
Objective:To investigate the effects of IAA on the development of lateral roots and IAA14 gene expression of Cyathula officinalis.Methods:The seeds of triennial Cyathula officinalis on geo-authentic habitats in Jinkou River of Leshan city were used as materials.Cyathula officinalis seedlings were cultured in MS medium containing different concentrations of IAA.The root microstructure,lateral root parameters,and IAA14 gene expression level were analysis.Results:The origin of lateral roots of Cyathula officinalis was the same as that of most dicotyledons.The lateral root primordium originated from the pericyclic cells directly opposite the xylem;IAA promoted the occurrence of lateral roots of Cyathula officinalis in a certain range.The IAA14 gene expression was down-regulated at the same time.The maximum number of average lateral root density appeared at an applied concentration of IAA with 1.83×10~(-5)mg/mL,and the expression of IAA14 gene was the lowest.Conclusion:The lateral roots of Cyathula officinalis originate from the pericyclic cells directly opposite the xylem,and IAA promotes the development of lateral roots of Cyathula officinalis in a certain range by real-time regulating the lateral root development-related IAA14 gene.
Objective:To investigate the effects of IAA on the development of lateral roots and IAA14 gene expression of Cyathula officinalis.Methods:The seeds of triennial Cyathula officinalis on geo-authentic habitats in Jinkou River of Leshan city were used as materials.Cyathula officinalis seedlings were cultured in MS medium containing different concentrations of IAA.The root microstructure,lateral root parameters,and IAA14 gene expression level were analysis.Results:The origin of lateral roots of Cyathula officinalis was the same as that of most dicotyledons.The lateral root primordium originated from the pericyclic cells directly opposite the xylem;IAA promoted the occurrence of lateral roots of Cyathula officinalis in a certain range.The IAA14 gene expression was down-regulated at the same time.The maximum number of average lateral root density appeared at an applied concentration of IAA with 1.83×10~(-5)mg/mL,and the expression of IAA14 gene was the lowest.Conclusion:The lateral roots of Cyathula officinalis originate from the pericyclic cells directly opposite the xylem,and IAA promotes the development of lateral roots of Cyathula officinalis in a certain range by real-time regulating the lateral root development-related IAA14 gene.
引文
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[2] 万德光,彭成,赵军宁.四川道地中药材志[M].成都:四川科学技术出版社,2005:44-47.
[3] 石圣洪,宋军,李祖伦,等.川牛膝本草考察[J].成都中医药大学学报,1998,21(4):4-6.
[4] 朱振兴,李丹.生长素信号对植物侧根发育的调控[J].中国细胞生物学学报,2015,37(11):1566-1571.
[5] Beeckman T,Burssens S,Inzé D.The peri-cell-cycle in Arabidopsis[J].Journal of Experimental Botany,2001,52:403-411.
[6] Casimiro I,Marchant A,Bhalerao RP,et al.Auxin transport promotes Arabidopsis lateral root initiation[J].Plant Cell,2001,13(4):843-852.
[7] 段莎莎,侯大斌,余马,等.北柴胡侧根发育及影响因素探究[J].中草药,2017,48(19):4062-4067.
[8] 李欣欣,赵静,廖红.吲哚乙酸、吲哚丁酸和萘乙酸对大豆幼根生长的影响[J].植物生理学报,2013,49(6):573-578.
[9] Mansfield SG,Briarty LG.Early embryogenesis in Arabidopsis thaliana.II.The developing embryo[J].Canadian Journal of Botany,1991,69(3):461-476.
[10] 邢国芳,冯万军,牛旭龙,等.植物激素调控侧根发育的生理机制[J].植物生理学报,2015,51(12):2101-2108.
[11] Okushima Y,Fukaki H,Onoda M,et al.ARF7 and ARF19 regulate lateral root formation via direct activation of LBD/ASL genes in Arabidopsis[J].Plant Cell,2007,19(1):118-130.
[12] Fukaki H,Nakao Y,Okushima Y,et al.Tissue-specific expression of stabilized SOLITARY-ROOT/IAA14 alters lateral root development in Arabidopsis[J].The Plant Journal,2005,44(3):382-395.
[13] Ulmasov T,Hagen G,Guilfoyle TJ.Activationand repressionof transcription by Auxin-responsefactors[J].Proceedingsof the National Academyof Sciences,1999,96(10):5844-5849.
[14] 朱青松,梅康凤,王沙生.外源生长素对烟草髓愈伤组织分化和内源IAA含量的影响[J].北京林业大学学报,1999,21(1):28-31.