菁松×皓月全蚕粉的成分测定与分析
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摘要
为探明菁松×皓月蚕品种5龄第3天全蚕粉中的1-DNJ、蛋白质、脂肪、可溶性总糖、锌、铁、磷、硒、钙、铜、锰及18种游离氨基酸的成分含量,分别采用高效液相色谱(HPLC)法以及相关国家标准所规定的检测方法,对主要成分1-DNJ及其它部分营养成分的含量进行了测定。结果表明,全蚕粉与桑叶中的1-DNJ、蛋白质、脂肪、可溶性总糖、5种矿质元素、17种游离氨基酸等成分含量差异极大;主要成分1-DNJ的质量分数比桑叶高303.39%,表明家蚕食用桑叶后对桑叶中的大部分成分有很高的富集作用。
This paper is to explore the contents of 1-DNJ,protein,fat,soluble sugar,zinc,iron,phosphorus,selenium,calcium,copper,manganese,and 18 kinds of free amino acids in the silkworm powder made by the third day in the 5 th instar of Jinsong×Haoyue.RP-HPLC and the methods mentioned in the corresponding national standard were used to detect the contents of 1-DNJ and other nutrients.The results showed that there was a significant difference in 1-DNJ,protein,fat,soluble sugar,5 kinds of mineral element and 17 kinds of free amino acids between silkworm powder and mulberry leaves,and the mass fraction of 1-DNJ in silkworm powder was 303.39%higher than that in the mulberry leaves.These results indicated that the silkworm had a high enrichment effect for most components of mulberry leaves.
This paper is to explore the contents of 1-DNJ,protein,fat,soluble sugar,zinc,iron,phosphorus,selenium,calcium,copper,manganese,and 18 kinds of free amino acids in the silkworm powder made by the third day in the 5 th instar of Jinsong×Haoyue.RP-HPLC and the methods mentioned in the corresponding national standard were used to detect the contents of 1-DNJ and other nutrients.The results showed that there was a significant difference in 1-DNJ,protein,fat,soluble sugar,5 kinds of mineral element and 17 kinds of free amino acids between silkworm powder and mulberry leaves,and the mass fraction of 1-DNJ in silkworm powder was 303.39%higher than that in the mulberry leaves.These results indicated that the silkworm had a high enrichment effect for most components of mulberry leaves.
引文
[1]李有贵,储一宁,钟石,等.59份野生桑桑叶中的DNJ含量及粗提物对α-糖苷酶的抑制活性[J].蚕业科学,2010,36(5):0729-0737.
[2]施新琴,肖辉,孙波,等.不同全蚕粉中的1-脱氧野尻霉素含量及其对小鼠血糖浓度的影响[J].蚕业科学,2009,35(1):111-115.
[3]叶晶晶,殷浩,孙波等.桑树中的1-脱氧野尻霉素含量变化规律研究[J].蚕业科学2009;35(4):722-727.
[4]施新琴,崔为正,裘立群,等.用反相高效液相色谱—紫外检测法测定1-脱氧野尻霉素的含量[J].蚕业科学,2006,32(1):146-149.
[5]中华人民共和国国家卫生和计划生育委员会.食品安全国家标准膨化食品:GB17401-2014[S].北京:中国标准出版社,2015:1-2.
[2]施新琴,肖辉,孙波,等.不同全蚕粉中的1-脱氧野尻霉素含量及其对小鼠血糖浓度的影响[J].蚕业科学,2009,35(1):111-115.
[3]叶晶晶,殷浩,孙波等.桑树中的1-脱氧野尻霉素含量变化规律研究[J].蚕业科学2009;35(4):722-727.
[4]施新琴,崔为正,裘立群,等.用反相高效液相色谱—紫外检测法测定1-脱氧野尻霉素的含量[J].蚕业科学,2006,32(1):146-149.
[5]中华人民共和国国家卫生和计划生育委员会.食品安全国家标准膨化食品:GB17401-2014[S].北京:中国标准出版社,2015:1-2.