人参内生菌提取物对硝酸盐胁迫下番茄幼苗生长及氮代谢相关指标的影响
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摘要
以番茄幼苗为试验材料,研究不同浓度(10μg/ml、5μg/ml和1μg/ml)的人参内生菌(Alternaria sp.)提取物在Ca(NO3)2胁迫(75 mmol/L)下对番茄幼苗生长及氮代谢相关指标的影响。结果表明,与清水对照相比,硝酸盐胁迫处理下番茄幼苗生长受到抑制,可溶性蛋白含量、脯氨酸含量、硝态氮含量和氮代谢相关酶活性均受到影响。人参内生菌提取物浓度为5μg/ml的处理明显缓解了硝酸盐胁迫对番茄幼苗生长造成的伤害,显著提高了可溶性蛋白含量、硝酸还原酶(NR)活性、谷氨酸合成酶(GOGAT)活性和谷氨酸脱氢酶(GDH)活性,并显著降低了脯氨酸含量,说明5μg/ml人参内生菌提取物处理对硝酸盐胁迫下番茄幼苗生长和氮代谢的调节效果最佳。
Three different concentrations of extracts from Ginseng endophyte were used to treat tomato seedlings,and the effects of extracts from Ginseng endophyte on the growth and nitrogen metabolism of tomato seedlings under Ca(NO_3)_2 stress were studied. The results showed that the growth of tomato seedlings under Ca(NO_3)_2 stress was inhibited,the soluble protein content,proline content,nitrate nitrogen content and the activity of enzymes related to nitrogen metabolism were all affected,compared with the control group. 5 μg/ml of extracts from endophyte could alleviate the injury of plant caused by Ca(NO_3)_2 stress,significantly improve the soluble protein content,NR activity,GOGAT activity and GDH activity,and significantly reduc the content of proline. This suggested that the treatment of irrigating 5 μg/ml endophyte extracts showed the best effects on the growth and nitrogen metabolism of tomato seedlings under nitrate stress.
Three different concentrations of extracts from Ginseng endophyte were used to treat tomato seedlings,and the effects of extracts from Ginseng endophyte on the growth and nitrogen metabolism of tomato seedlings under Ca(NO_3)_2 stress were studied. The results showed that the growth of tomato seedlings under Ca(NO_3)_2 stress was inhibited,the soluble protein content,proline content,nitrate nitrogen content and the activity of enzymes related to nitrogen metabolism were all affected,compared with the control group. 5 μg/ml of extracts from endophyte could alleviate the injury of plant caused by Ca(NO_3)_2 stress,significantly improve the soluble protein content,NR activity,GOGAT activity and GDH activity,and significantly reduc the content of proline. This suggested that the treatment of irrigating 5 μg/ml endophyte extracts showed the best effects on the growth and nitrogen metabolism of tomato seedlings under nitrate stress.
引文
[1]程玉静,郭世荣,孙锦,等.外源硝酸钙对盐胁迫下黄瓜幼苗氮化合物含量和硝酸还原酶活性的影响[J].西北农业学报,2010,19(4):188-206.
[2]李琴,苏天明,王日升,等.广西设施栽培土壤次生盐渍化调查及其影响因子分析[J].江苏农业科学,2016,44(11):492-494.
[3]张飞,梁燕.番茄对盐胁迫的反应及其耐盐性鉴定的研究进展[J].长江蔬菜(学术版),2011(4):1-4.
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[5]尹燕东,魏珉,邹永洲,等.CO2施肥对黄瓜幼苗根系发育及氮代谢酶活性的影响[J].西北农业学报,2006,15(5):191-194,201.
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[7]赵媛,卢凤英.作物内生菌研究进展[J].江苏农业科学,2015,43(10):20-22.
[8]杨波,陈晏,李霞,等.植物内生菌促进宿主氮吸收与代谢研究进展[J].生态学报,2013,33(9):2656-2664.
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[10]袁志林,戴传超,史央,等.内生真菌B3促进水稻生长的机理研究[J].江苏农业科学,2004(2):10-13.
[11]BATES L S,WALDREN R P,TEARE I D.Rapid determination of free proline for water-stress studies[J].Plant and Soil,1973,39(1):205-207.
[12]张以顺,黄霞,陈云凤,等.植物生理学实验教程[M].北京:高等教育出版社,2009:85,88-89.
[13]SRIVASTAVA H S,ORMROD D P.Effects of nitrogen dioxide and nitrate nutrition on growth and nitrate assimilation in bean leaves[J].Plant Physiology,1984,76(2):418-423.
[14]王娜,杨镇,曹君,等.植物内生菌提取物对水稻生长发育的影响[J].吉林农业大学学报,2014,36(1):10-16.
[15]肇莹,杨镇,杨涛,等.植物内生菌醇提取物对草坪草抗旱性的影响[J].江苏农业学报,2015,31(1):39-44.
[16]王朝辉,李生秀,田霄鸿.不同氮肥用量对蔬菜硝酸盐累积的影响[J].植物营养与肥料学报,1998,4(1):22-28.
[17]李顺,尤娟,徐晋豫,等.樱桃番茄幼苗对硝酸盐胁迫的生长和生理响应[J].西北植物学报,2014,34(2):332-340.
[18]费伟,陈火英,曹忠,等.盐胁迫对番茄幼苗生理特性的影响[J].上海交通大学学报(农业科学版),2005,23(1):5-9,30.
[19]刘书仁,郭世荣,刘超杰,等.脯氨酸对高温胁迫下黄瓜氮化合物含量和硝酸还原酶活性的影响[J].江苏农业学报,2011,27(2):366-370.
[20]杨荣,邱炜红,王朝辉,等.硝酸还原酶抑制剂钨酸钠对油菜硝态氮积累的影响[J].植物生理学报,2012,48(1):51-56.
[21]张以顺,黄霞,陈云凤,等.植物生理学实验教程[M].北京:高等教育出版社,2009:90.
[22]张毅,石玉,胡晓辉,等.外源对盐碱胁迫下番茄幼苗氮代谢及主要矿质元素含量的影响[J].应用生态学报,2013,24(5):1401-1408.
[23]周万海,师尚礼,寇江涛,等.盐胁迫下外源NO对苜蓿幼苗生长及氮代谢的影响[J].应用生态学报,2012,23(11):3003-3008.
[2]李琴,苏天明,王日升,等.广西设施栽培土壤次生盐渍化调查及其影响因子分析[J].江苏农业科学,2016,44(11):492-494.
[3]张飞,梁燕.番茄对盐胁迫的反应及其耐盐性鉴定的研究进展[J].长江蔬菜(学术版),2011(4):1-4.
[4]潘瑞炽.植物生理学[M].北京:高等教育出版社,2004:410-413.
[5]尹燕东,魏珉,邹永洲,等.CO2施肥对黄瓜幼苗根系发育及氮代谢酶活性的影响[J].西北农业学报,2006,15(5):191-194,201.
[6]赵福庚,刘友良.胁迫条件下高等植物体内脯氨酸代谢及调节的研究进展[J].植物学通报,1999,16(5):540-546.
[7]赵媛,卢凤英.作物内生菌研究进展[J].江苏农业科学,2015,43(10):20-22.
[8]杨波,陈晏,李霞,等.植物内生菌促进宿主氮吸收与代谢研究进展[J].生态学报,2013,33(9):2656-2664.
[9]LYONS P C,EVANS J J,BACON C W.Effects of the fungal endophyte Acremonium coenophialum on nitrogen accumulation and metabolism in tall fescue[J].Plant Physiology,1990,92:726-732.
[10]袁志林,戴传超,史央,等.内生真菌B3促进水稻生长的机理研究[J].江苏农业科学,2004(2):10-13.
[11]BATES L S,WALDREN R P,TEARE I D.Rapid determination of free proline for water-stress studies[J].Plant and Soil,1973,39(1):205-207.
[12]张以顺,黄霞,陈云凤,等.植物生理学实验教程[M].北京:高等教育出版社,2009:85,88-89.
[13]SRIVASTAVA H S,ORMROD D P.Effects of nitrogen dioxide and nitrate nutrition on growth and nitrate assimilation in bean leaves[J].Plant Physiology,1984,76(2):418-423.
[14]王娜,杨镇,曹君,等.植物内生菌提取物对水稻生长发育的影响[J].吉林农业大学学报,2014,36(1):10-16.
[15]肇莹,杨镇,杨涛,等.植物内生菌醇提取物对草坪草抗旱性的影响[J].江苏农业学报,2015,31(1):39-44.
[16]王朝辉,李生秀,田霄鸿.不同氮肥用量对蔬菜硝酸盐累积的影响[J].植物营养与肥料学报,1998,4(1):22-28.
[17]李顺,尤娟,徐晋豫,等.樱桃番茄幼苗对硝酸盐胁迫的生长和生理响应[J].西北植物学报,2014,34(2):332-340.
[18]费伟,陈火英,曹忠,等.盐胁迫对番茄幼苗生理特性的影响[J].上海交通大学学报(农业科学版),2005,23(1):5-9,30.
[19]刘书仁,郭世荣,刘超杰,等.脯氨酸对高温胁迫下黄瓜氮化合物含量和硝酸还原酶活性的影响[J].江苏农业学报,2011,27(2):366-370.
[20]杨荣,邱炜红,王朝辉,等.硝酸还原酶抑制剂钨酸钠对油菜硝态氮积累的影响[J].植物生理学报,2012,48(1):51-56.
[21]张以顺,黄霞,陈云凤,等.植物生理学实验教程[M].北京:高等教育出版社,2009:90.
[22]张毅,石玉,胡晓辉,等.外源对盐碱胁迫下番茄幼苗氮代谢及主要矿质元素含量的影响[J].应用生态学报,2013,24(5):1401-1408.
[23]周万海,师尚礼,寇江涛,等.盐胁迫下外源NO对苜蓿幼苗生长及氮代谢的影响[J].应用生态学报,2012,23(11):3003-3008.