摘要
探索了以紫色红曲霉(Monascus purpureus)固态发酵豆渣产红曲色素的可行性。从5种不同原料中筛选出豆渣为产红曲色素的最适固态发酵基质;在单因素实验的基础上,采用正交实验优化法得出紫色红曲霉固态发酵豆渣产红曲色素的最佳培养基组成为基质初始含水量50%、甘油6%、NaNO_30. 04%、KH_2PO_40. 3%、MgSO_40. 2%、抗坏血酸2. 2%,最佳培养条件为湿度60%~65%、接种量8%、30℃培养12 d,在此条件下红曲色素的含量为(6. 03±0. 11) mg/g。研究认为,以紫色红曲霉固态发酵豆渣产红曲色素的工艺可行,可实现豆渣的高值化发酵再利用。
Feasibility for the production of Monascus pigments by solid-state fermentation of soybean dregs with Monascus purpureus was investigated. Soybean dregs was selected as the optimum solid-state fermentation medium for producing Monascus pigments from five different raw materials. On the basis of single factor experiment,the optimal medium composition for the production of Monascus pigments by solid-state fermentation of soybean dregs with Monascus purpureus was obtained by orthogonal experiment as follows: initial moisture content of soybean dregs 50%,glycerol 6%,NaNO30. 04%,KH_2PO_40. 3%,MgSO_4 0. 2% and VC2. 2%. The optimal culture conditions were obtained as follows: humidity 60%-65%,inoculation amount 8%,fermentation time 12 d and fermentation temperature 30 ℃. Under the optimal conditions,the content of Monascus pigments in fermented soybean dregs was( 6. 03 ± 0. 11)mg/g. The results indicated that it was feasible to produce Monascus pigments by solid-state fermentation of soybean dregs with Monascus purpureus to achieve the reutilization of soybean dregs with high-value through fermentation process.
引文
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