近平滑假丝酵母补糖发酵及糖氮代谢对产甘露醇的影响
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摘要
为了探究近平滑假丝酵母(Candida parapsilosis)生长及代谢过程中对碳源和氮源的需求,本文对发酵各阶段的碳源和氮源变化利用情况进行检测,并设定一个空白对照组和四个不同时期的补糖实验组进行研究,分别观测各实验组各时期生物量和甘露醇产量。结果表明,在72 h时进行补糖为最佳时期,甘露醇产量达到最大值为60.28 g/L,相比于空白对照组高出19.82%。通过补糖工艺的研究,在氮源和碳源耗尽时,补糖可以促进甘露醇的产生。本文为以后的工业化生产提供了理论依据。
In order to explore the demand for carbon and nitrogen sources of Candida parapsilosis during the growth and metabolism,the charge and utilization of carbon and nitrogen sources at different stages of fermentation were determined,and a blank control group and four different periods of glucose supplement test groups were set up.The biomass and mannitol yield of each experiment group were measured respectively.The research showed that it was the optimal time to adding oligosaccharide when fermented for 72 h,and the yield of mannitol reached the maximum value 60.28 g/L which increased by 19.82%compared with the control group.Through the study of the sugar supplement process,when the nitrogen and sugar source was used up,the addition of glucose can promote the production of mannitol. This study provided a theoretical basis for the future industrial production.
In order to explore the demand for carbon and nitrogen sources of Candida parapsilosis during the growth and metabolism,the charge and utilization of carbon and nitrogen sources at different stages of fermentation were determined,and a blank control group and four different periods of glucose supplement test groups were set up.The biomass and mannitol yield of each experiment group were measured respectively.The research showed that it was the optimal time to adding oligosaccharide when fermented for 72 h,and the yield of mannitol reached the maximum value 60.28 g/L which increased by 19.82%compared with the control group.Through the study of the sugar supplement process,when the nitrogen and sugar source was used up,the addition of glucose can promote the production of mannitol. This study provided a theoretical basis for the future industrial production.
引文
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[9]王虎玄,胡仲秋,牛晨,等.糖度与酸度对鲁氏接合酵母生长的影响[J].农业机械学报,2015(10):279-284.
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[12]佘隽,田华,陈涛,等.高产DHA寇氏隐甲藻突变株的筛选[J].食品科学,2013,34(17):230-235.
[13]赵书林,何东平,陈涛,等.寇氏隐甲藻突变株的糖、氮代谢对产油的影响[J].中国粮油学报,2014,29(11):65-69.
[14]潘峰,王一健,孙玮,等.高效液相色谱法测定人尿乳果糖及甘露醇[J].第三军医大学学报,1998(6):86-88.
[15]Gaspar P,Neves A R,Ramos A,et al.Engineering lactococcus lactis for production of manitol:high yields from food-grade strains deficient in lactate dehydrogenase and the mannitol transport system[J].Applied and Environmental Microbiology,2004,70(3):1466-1474.
[2]林琳,岳敏,曹海龙,等.产甘露醇菌株的育种研究进展[J].食品工业科技,2012,33(11):426-430.
[3]潘欣.1株近平滑假丝酵母的分离及其鉴定[J].微生物学杂志,2011,31(1):43-46.
[4]艾丽静,饶志明,沈微,等.产还原型谷胱甘肽酵母菌的筛选与初步鉴定[J].食品与发酵业,2006,32(3):18-21.
[5]张荣珍.近平滑假丝酵母立体选择性还原酶表达、结构解析与改造[D].无锡:江南大学,2009.
[6]曲有鹏.近平滑假丝酵母木糖还原酶基因的克隆及表达研究[D].哈尔滨:哈尔滨工业大学,2007.
[7]娄文勇,郭强,郁惠蕾,等.近平滑假丝酵母细胞催化乙酰基三甲基硅烷不对称还原反应[J].催化学报,2009,30(12):1276-1280.
[8]朱豫,曹海龙,岳敏,等.以菊芋为原料发酵生产甘露醇的研究[J].西北农业学报,2009(5):137-141.
[9]王虎玄,胡仲秋,牛晨,等.糖度与酸度对鲁氏接合酵母生长的影响[J].农业机械学报,2015(10):279-284.
[10]魏文婷.产甘露醇菌株的筛选及其生物合成的研究[D].无锡:江南大学,2014.
[11]M.de Swaaf,J.Pronk,L Sijtsma.Fed-batch cultivation of the docosahexaenoic-acid-producing marine alga Crypthecodinium cohnii on ethanol[J].Applied Microbiology and Biotechnology,2003,61(1):40-43.
[12]佘隽,田华,陈涛,等.高产DHA寇氏隐甲藻突变株的筛选[J].食品科学,2013,34(17):230-235.
[13]赵书林,何东平,陈涛,等.寇氏隐甲藻突变株的糖、氮代谢对产油的影响[J].中国粮油学报,2014,29(11):65-69.
[14]潘峰,王一健,孙玮,等.高效液相色谱法测定人尿乳果糖及甘露醇[J].第三军医大学学报,1998(6):86-88.
[15]Gaspar P,Neves A R,Ramos A,et al.Engineering lactococcus lactis for production of manitol:high yields from food-grade strains deficient in lactate dehydrogenase and the mannitol transport system[J].Applied and Environmental Microbiology,2004,70(3):1466-1474.