五味消毒饮中大孔树脂分离部位的体外抗菌活性研究
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摘要
目的检测五味消毒饮不同分离部位的体外抗菌活性,为五味消毒饮的二次开发提供实验依据。方法本研究将五味消毒饮水煎液用NKA-9大孔树脂进行吸附分离得到不同部位的洗脱液,用牛津杯法测定其抑菌圈,并用试管稀释法检测洗脱液对细菌的最低抑菌浓度(MIC)。结果各分离部位对不同菌株的抑菌效果有显著差异,30%乙醇洗脱液对金黄色葡萄球菌和志贺氏痢疾杆菌的抑制效果最好,MIC分别为0.055 g/m L和0.150 g/m L;而水洗液对肺炎链球菌杆菌的抑制效果最佳,MIC为0.184 g/m L,对金黄色葡萄球菌和志贺氏痢疾杆菌没有抑制作用;水洗液、10%乙醇洗脱液、30%乙醇洗脱液对绿脓杆菌几乎没有抑制效果,极性稍小的50%乙醇洗脱液、70%乙醇洗脱液、90%乙醇洗脱液对绿脓杆菌有较弱的抑制效果。结论五味消毒饮的不同分离部位对不同细菌的抑制效果不同,为经典方剂"五味消毒饮"的二次开发提供了理论依据和数据支持。
Objective To investigate the in vitro antibacterial activity of different separated parts of Wuwei Xiaodu Decoction, and to provide an experimental basis for the secondary development of Wuwei Xiaodu Decoction. Methods The water extract solution of Wuwei Xiaodu Decoction was absorbed and separated by NKA-9 macroporous resin to obtain the eluent of each part. The Oxford cup method was used to measure the inhibition zone, and the tube dilution method was used to measure the minimal inhibitory concentration(MIC) of the eluent. Results There was a significant difference in antibacterial effect on different strains between the separated parts. The 30% ethanol eluent had the best inhibitory effect on Staphylococcus aureus and Shigella dysenteriae, with an MIC of 0.055 g/ml and 0.150 g/ml, respectively. The water extract had the best inhibitory effect on Streptococcus pneumoniae, with an MIC of 0.184 g/ml, while it had no inhibitory effect on Staphylococcus aureus and Shigella dysenteriae. The water extract, 10% ethanol eluent, and 30% ethanol eluent had little inhibitory effect on Pseudomonas aeruginosa, and 50% ethanol eluent with less polarity, 70% ethanol eluent, and 90% ethanol eluent had a weak inhibitory effect on Pseudomonas aeruginosa. Conclusion Different separated parts of Wuwei Xiaodu Decoction have a different inhibitory effect on different bacteria, which provide a theoretical basis and data support for the secondary development of the classic prescription Wuwei Xiaodu Decoction.
Objective To investigate the in vitro antibacterial activity of different separated parts of Wuwei Xiaodu Decoction, and to provide an experimental basis for the secondary development of Wuwei Xiaodu Decoction. Methods The water extract solution of Wuwei Xiaodu Decoction was absorbed and separated by NKA-9 macroporous resin to obtain the eluent of each part. The Oxford cup method was used to measure the inhibition zone, and the tube dilution method was used to measure the minimal inhibitory concentration(MIC) of the eluent. Results There was a significant difference in antibacterial effect on different strains between the separated parts. The 30% ethanol eluent had the best inhibitory effect on Staphylococcus aureus and Shigella dysenteriae, with an MIC of 0.055 g/ml and 0.150 g/ml, respectively. The water extract had the best inhibitory effect on Streptococcus pneumoniae, with an MIC of 0.184 g/ml, while it had no inhibitory effect on Staphylococcus aureus and Shigella dysenteriae. The water extract, 10% ethanol eluent, and 30% ethanol eluent had little inhibitory effect on Pseudomonas aeruginosa, and 50% ethanol eluent with less polarity, 70% ethanol eluent, and 90% ethanol eluent had a weak inhibitory effect on Pseudomonas aeruginosa. Conclusion Different separated parts of Wuwei Xiaodu Decoction have a different inhibitory effect on different bacteria, which provide a theoretical basis and data support for the secondary development of the classic prescription Wuwei Xiaodu Decoction.
引文
[1]郁韶明.五味消毒饮临床新应用[J].中华现代中医学杂志,2010,6(1):38-40.
[2]向敏,王建梅,顾瑶华.加味五味消毒饮提取物对小鼠角叉菜胶炎症模型的作用[J].中国现代医学杂志,2011,21(12):1462-1465.
[3]游俊,王英夫.五味消毒饮内服外敷治疗寻常性痤疮35例临床观察[J].中国医院药学杂志,2012,32(8):626-627.
[4]郭丽娜.五味消毒饮合仙方活命饮耳鼻喉科疾病的体会[J].时珍国医国药,2009,20(8):2068-2068.
[5]陈雅文.浅谈金银花的化学成分、药理作用和实用价值[J].中药研究,2013,21(5):70-71.
[6]毕跃峰,潘成学,王普菊,等.野菊花化学成分的研究[J].中国药学杂志,2009,44(12):894-897.
[7]向福,冯鹏,刘亮,等.大孔树脂对罗田金银花叶中绿原酸的分离研究[J].食品与机械,2013,29(4):141-144.
[8]张默,霍清.利用大孔树脂提取绿原酸的研究[J].安徽农业科学,2009,37(17):8167-8168,8192.
[9]张世华,曾秀,付丽芝,等.五味消毒饮及各味药物对化脓性细菌的抑菌试验与病理模型疗效观察[J].畜禽业,2000(6):22-24.
[10]李永梅.金银花的抗腺病毒作用研究[J].华西药学杂志,2001,16(5):327-329.
[11]黄水仙,田道法,江志超.五味消毒饮治疗大鼠实验性急性鼻咽炎的疗效观察[J].中国中西医结合耳鼻咽喉科杂志,2006,14(1):11-13.
[12]王芝英,肖榕,张林,等.五味消毒饮对化脓性细菌的抑菌试验[J].四川畜牧兽医,2003,30(6):20-21.
[13]孔宪琴,梁航,杨国胜.五味消毒饮对猪链球菌病病原体的体外抑菌试验[J].中兽医学杂志,2009(3):10-13.
[14]唐敏,刘耀,王渝,等.金银花总黄酮粗提物体外抑菌作用研究[J].中国药房,2008,19(30):2321-2323.
[15]吴海英.绿原酸对粘液型铜绿假单胞菌生物膜的干预以及对左氧氟沙星增效作用[D].南宁:广西医科大学,2011.
[16]赵行才,陈晓.50例湿疹患者皮损部位定植细菌及其药敏试验分析[J].浙江临床医学,2009,l1(2):138-139.
[2]向敏,王建梅,顾瑶华.加味五味消毒饮提取物对小鼠角叉菜胶炎症模型的作用[J].中国现代医学杂志,2011,21(12):1462-1465.
[3]游俊,王英夫.五味消毒饮内服外敷治疗寻常性痤疮35例临床观察[J].中国医院药学杂志,2012,32(8):626-627.
[4]郭丽娜.五味消毒饮合仙方活命饮耳鼻喉科疾病的体会[J].时珍国医国药,2009,20(8):2068-2068.
[5]陈雅文.浅谈金银花的化学成分、药理作用和实用价值[J].中药研究,2013,21(5):70-71.
[6]毕跃峰,潘成学,王普菊,等.野菊花化学成分的研究[J].中国药学杂志,2009,44(12):894-897.
[7]向福,冯鹏,刘亮,等.大孔树脂对罗田金银花叶中绿原酸的分离研究[J].食品与机械,2013,29(4):141-144.
[8]张默,霍清.利用大孔树脂提取绿原酸的研究[J].安徽农业科学,2009,37(17):8167-8168,8192.
[9]张世华,曾秀,付丽芝,等.五味消毒饮及各味药物对化脓性细菌的抑菌试验与病理模型疗效观察[J].畜禽业,2000(6):22-24.
[10]李永梅.金银花的抗腺病毒作用研究[J].华西药学杂志,2001,16(5):327-329.
[11]黄水仙,田道法,江志超.五味消毒饮治疗大鼠实验性急性鼻咽炎的疗效观察[J].中国中西医结合耳鼻咽喉科杂志,2006,14(1):11-13.
[12]王芝英,肖榕,张林,等.五味消毒饮对化脓性细菌的抑菌试验[J].四川畜牧兽医,2003,30(6):20-21.
[13]孔宪琴,梁航,杨国胜.五味消毒饮对猪链球菌病病原体的体外抑菌试验[J].中兽医学杂志,2009(3):10-13.
[14]唐敏,刘耀,王渝,等.金银花总黄酮粗提物体外抑菌作用研究[J].中国药房,2008,19(30):2321-2323.
[15]吴海英.绿原酸对粘液型铜绿假单胞菌生物膜的干预以及对左氧氟沙星增效作用[D].南宁:广西医科大学,2011.
[16]赵行才,陈晓.50例湿疹患者皮损部位定植细菌及其药敏试验分析[J].浙江临床医学,2009,l1(2):138-139.