肠黏膜自然杀伤细胞的免疫功能及其调控
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摘要
自然杀伤(NK)细胞是肠黏膜免疫屏障的重要组分。本文从NK细胞的发育学特征、分子表型切入,分析了其在维护肠道屏障完整性中发挥的功能,以及肠道微生物对其免疫功能的调节作用,旨在为疾病预防和肠道健康的营养调控提供理论依据。
Natural killer( NK) cells are important components of the intestinal mucosal immune barrier. In this paper,based on the developmental characteristics and molecular phenotypes of NK cells,their functions in maintaining intestinal barrier integrity and the regulation of intestinal microbes on their immune function are reviewed,aiming to provide theory basis for the nutrition regulation of disease prevention and intestinal health.
Natural killer( NK) cells are important components of the intestinal mucosal immune barrier. In this paper,based on the developmental characteristics and molecular phenotypes of NK cells,their functions in maintaining intestinal barrier integrity and the regulation of intestinal microbes on their immune function are reviewed,aiming to provide theory basis for the nutrition regulation of disease prevention and intestinal health.
引文
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[26]STORSET A K,KULBERG S,BERG I,BOYSEN P,et al.NKp46 defines a subset of bovine leukocytes with natural killer cell characteristics[J].European Journal of Immunology,2004,34(3):669-676.
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[31]FINK L N,ZEUTHEN L H,CHRISTENSEN H R,et al.Distinct gut-derived lactic acid bacteria elicit divergent dendritic cell-mediated NK cell responses[J].International Immunology,2007,19(12):1319-1327.
[32]LEN F,ROLDN E,SANCHEZ L,et al.Human small-intestinal epithelium contains functional natural killer lymphocytes[J].Gastroenterology,2003,125(2):345-356.
[33]SIVORI S,FALCO M,CHIESA M D,et al.CpG and double-stranded RNA trigger human NK cells by tolllike receptors:induction of cytokine release and cytotoxicity against tumors and dendritic cells[J].Proceedings of the National Academy of Sciences of the United States of America,2004,101(27):10116-10121.
[34]ZHANG J H,DENG J,WANG Z S,et al.Modulatory effects of Lactobacillus salivarius on intestinal mucosal immunity of piglets[J].Current Microbiology,2011,62(5):1623-1631.
[35]WANG A N,YU H F,GAO X,et al.Influence of Lactobacillus fermentum I5007 on the intestinal and systemic immune responses of healthy and E.coli challenged piglets[J].Antonie van Leeuwenhoek,2009,96(1):89-98.
[36]PARK J H,UM J I,LEE B J,et al.Encapsulated Bifidobacterium bifidum potentiates intestinal IgA production[J].Cellular Immunology,2002,219(1):22-27.
[37]COSTABILE A,BERGILLOS-MECA T,RASINKAN-GAS P,et al.Effects of soluble corn fiber alone or in synbiotic combination with Lactobacillus rhamnosus GGand the pilus-deficient derivative GG-PB12 on fecal microbiota,metabolism,and markers of immune function:a randomized,double-blind,placebo-controlled,crossover study in healthy elderly[J].Frontiers in Immunology,2017,8:1443.
[38]OGAWA T,ASAI Y,TAMAI R,et al.Natural killer cell activities of synbiotic Lactobacillus casei ssp.casei in conjunction with dextran[J].Clinical and Experimental Immunology,2005,143(1):103-109.
[39]QIU Y Q,JIANG Z Y,HU S L,et al.Lactobacillus plantarum enhanced IL-22 production in natural killer(NK)cells that protect the integrity of intestinal epithelial cell barrier damaged by enterotoxigenic Escherichia coli[J].International Journal of Molecular Sciences,2017,18(11):2409.
[2]SATOH-TAKAYAMA N,VOSSHENRICH C A J,LESJEAN-POTTIER S,et al.Microbial flora drives interleukin 22 production in intestinal NKp46+cells that provide innate mucosal immune defense[J].Immunity,2008,29(6):958-970.
[3]CELLA M,FUCHS A,VERMI W,et al.A human natural killer cell subset provides an innate source of IL-22for mucosal immunity[J].Nature,2009,457(7230):722-725.
[4]STMAN S,Jr,RASK C,WOLD A E,et al.Impaired regulatory T cell function in germ-free mice[J].European Journal of Immunology,2006,36(9):2336-2346.
[5]LEE J S,AWJI E G,LEE S J,et al.Effect of Lactobacillus plantarum CJLP243 on the growth performance and cytokine response of weaning pigs challenged with enterotoxigenic Escherichia coli[J].Journal of Animal Science,2012,90(11):3709-3717.
[6]BUERL C,LLOPIS M,ANTOLN M,et al.Lactobacillus paracasei and Lactobacillus plantarum strains downregulate proinflammatory genes in an ex vivo system of cultured human colonic mucosa[J].Genes&Nutrition,2013,8(2):165-180.
[7]YANG K M,JANG Z Y,ZHENG C T,et al.Effect of Lactobacillus plantarum on diarrhea and intestinal barrier function of young piglets challenged with enterotoxigenic Escherichia coli K88[J].Journal of Animal Science,2014,92(4):1496-1503.
[8]SUN J C,LANIER L L.NK cell development,homeostasis and function:parallels with CD8+T cells[J].Nature Reviews Immunology,2011,11(10):645-657.
[9]SHI F D,LJUNGGREN H G,LA CAVA A,et al.Organspecific features of natural killer cells[J].Nature Reviews Immunology,2011,11(10):658-671.
[10]SUN J C,BEILKE J N,LANIER L L.Adaptive immune features of natural killer cells[J].Nature,2009,457(7229):557-561.
[11]GBEL T W F,KASPERS B,STANGASSINGER M.NK and T cells constitute two major,functionally distinct intestinal epithelial lymphocyte subsets in the chicken[J].International Immunology,2001,13(6):757-762.
[12]HARTY J T,BADOVINAC V P.Shaping and reshaping CD8+T-cell memory[J].Nature Reviews Immunology,2008,8(2):107-119.
[13]WILLIAMS M A,BEVAN M J.Effector and memory CTL differentiation[J].Annual Review of Immunology,2007,25:171-192.
[14]GOLDRATH A W,BEVAN M J.Selecting and maintaining a diverse T-cell repertoire[J].Nature,1999,402(6759):255-262.
[15]BADOVINAC V P,PORTER B B,HARTY J T.Programmed contraction of CD8+T cells after infection[J].Nature Immunology,2002,3(7):619-626.
[16]MASOPUST D,VEZYS V,MARZO A L,et al.Preferential localization of effector memory cells in nonlymphoid tissue[J].Science,2001,291(5512):2413-2417.
[17]CHAI J Y,LILLEHOJ H S.Isolation and functional characterization of chicken intestinal intra-epithelial lymphocytes showing natural killer cell activity against tumour target cells[J].Immunology,1988,63(1):111-117.
[18]FERNANDEZ N C,LOZIER A,FLAMENT C,et al.Dendritic cells directly trigger NK cell functions:crosstalk relevant in innate anti-tumor immune responses in vivo[J].Nature Medicine,1999,5(4):405-411.
[19]WALZER T,DALOD M,ROBBINS S H,et al.Naturalkiller cells and dendritic cells:“l’union fait la force”[J].Blood,2005,106(7):2252-2258.
[20]HOLTMEIER W,KABELITZ D.Gammadelta T cells link innate and adaptive immune responses[J].Chemical Immunology and Allergy,2005,86:151-183.
[21]MANIAR A,ZHANG X Y,LIN W,et al.HumanγδTlymphocytes induce robust NK cell-mediated antitumor cytotoxicity through CD137 engagement[J].Blood,2010,116(10):1726-1733.
[22]田志刚,魏海明,孙汭.NK细胞免疫识别及其调节机制与免疫相关性疾病[J].中国科学技术大学学报,2008,38(8):896-904.
[23]RIZZELLO V,BONACCORSI I,DONGARRM L,et al.Role of natural killer and dendritic cell crosstalk in immunomodulation by commensal bacteria probiotics[J].Journal of Biomedicine and Biotechnology,2011,2011:473097.
[24]KUMAR P,THAKAR M S,OUYANG W,et al.IL-22from conventional NK cells is epithelial regenerative and inflammation protective during influenza infection[J].Mucosal Immunology,2013,6(1):69-82.
[25]OLSEN L,BOYSEN P,KESSON C P,et al.Characterization of NCR1+cells residing in lymphoid tissues in the gut of lambs indicates that the majority are NK cells[J].Veterinary Research,2013,44:109.
[26]STORSET A K,KULBERG S,BERG I,BOYSEN P,et al.NKp46 defines a subset of bovine leukocytes with natural killer cell characteristics[J].European Journal of Immunology,2004,34(3):669-676.
[27]KOREN H S,AMOS D B,KIM Y B.Natural killing and antibody-dependent cellular cytotoxicity are independent immune functions in the Minnesota miniature swine[J].Proceedings of the National Academy of Sciences of the United States of America,1978,75(10):5127-5131.
[28]YANG W C,SCHULTZ R D.Ontogeny of natural killer cell activity and antibody dependent cell mediated cytotoxicity in pigs[J].Developmental&Comparative Immunology,1986,10(3):405-418.
[29]EYERICH K,DIMARTINO V,CAVANI A.IL-17 and IL-22 in immunity:driving protection and pathology[J].European Journal of Immunology,2017,47(4):607-614.
[30]SONNENBERG G F,MONTICELLI L A,ELLOSO MM,et al.CD4+lymphoid tissue-inducer cells promote innate immunity in the gut[J].Immunity,2011,34(1):122-134.
[31]FINK L N,ZEUTHEN L H,CHRISTENSEN H R,et al.Distinct gut-derived lactic acid bacteria elicit divergent dendritic cell-mediated NK cell responses[J].International Immunology,2007,19(12):1319-1327.
[32]LEN F,ROLDN E,SANCHEZ L,et al.Human small-intestinal epithelium contains functional natural killer lymphocytes[J].Gastroenterology,2003,125(2):345-356.
[33]SIVORI S,FALCO M,CHIESA M D,et al.CpG and double-stranded RNA trigger human NK cells by tolllike receptors:induction of cytokine release and cytotoxicity against tumors and dendritic cells[J].Proceedings of the National Academy of Sciences of the United States of America,2004,101(27):10116-10121.
[34]ZHANG J H,DENG J,WANG Z S,et al.Modulatory effects of Lactobacillus salivarius on intestinal mucosal immunity of piglets[J].Current Microbiology,2011,62(5):1623-1631.
[35]WANG A N,YU H F,GAO X,et al.Influence of Lactobacillus fermentum I5007 on the intestinal and systemic immune responses of healthy and E.coli challenged piglets[J].Antonie van Leeuwenhoek,2009,96(1):89-98.
[36]PARK J H,UM J I,LEE B J,et al.Encapsulated Bifidobacterium bifidum potentiates intestinal IgA production[J].Cellular Immunology,2002,219(1):22-27.
[37]COSTABILE A,BERGILLOS-MECA T,RASINKAN-GAS P,et al.Effects of soluble corn fiber alone or in synbiotic combination with Lactobacillus rhamnosus GGand the pilus-deficient derivative GG-PB12 on fecal microbiota,metabolism,and markers of immune function:a randomized,double-blind,placebo-controlled,crossover study in healthy elderly[J].Frontiers in Immunology,2017,8:1443.
[38]OGAWA T,ASAI Y,TAMAI R,et al.Natural killer cell activities of synbiotic Lactobacillus casei ssp.casei in conjunction with dextran[J].Clinical and Experimental Immunology,2005,143(1):103-109.
[39]QIU Y Q,JIANG Z Y,HU S L,et al.Lactobacillus plantarum enhanced IL-22 production in natural killer(NK)cells that protect the integrity of intestinal epithelial cell barrier damaged by enterotoxigenic Escherichia coli[J].International Journal of Molecular Sciences,2017,18(11):2409.