浙江省南美白对虾苗种五种流行病病原检测与分析
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摘要
为了解浙江省南美白对虾主产区苗种主要病原的携带情况,对杭州、宁波、嘉兴和绍兴4个地区的苗种场开展了病原检测。129批次样品的检测结果显示:有4种病原检出阳性,阳性检出率分别为南美白对虾白斑综合症病毒(WSSV)1.55%、传染性皮下及造血组织坏死病毒(IHHNV)20.93%、虾肝肠胞虫(EHP)31.78%、虾血细胞虹彩病毒(SHIV)37.21%,而桃拉综合症病毒(TSV)未有阳性检出。结果表明,SHIV、EHP、IHHNV是浙江省对虾苗种携带的主要病原,需要重点加以防范。从整体分布看,宁波市南美白对虾苗中携带的病原种类最多,且携带率较高,提示宁波市需要加强防控。从苗种来源看,来自福建省虾苗的IHHNV携带率最高,为38.10%;来自海南省和广东省虾苗中存在WSSV感染,检出率分别为2.17%和2.27%;来自广东省虾苗的EHP和SHIV携带率最高,分别为43.48%和41.30%。由于浙江省南美白对虾苗种主要依靠外地输入,而这些输出地区的苗种病原感染率较高,因此浙江省需要加强从这些地区引入苗种的检疫工作。
In order to recognize the main pathogens carried by white shrimp(Penaeus vannamei)in Zhejiang Province,a total of 129 samples were collected from shrimp seed hatcheries in 4 cities of Hangzhou,Ningbo,Jiaxing and Shaoxin and used for pathogen detection. According to the results,four kinds of pathogens were detected,including white spot syndrome virus(WSSV),infectious hypodermal and hematopoietic necrosis virus(IHHNV),enterocytozoom hepatopenaei(EHP)and shrimp hemocyte iridescent virus(SHIV). The results showed that the positive rate of WSSV was 1.55%,while the rates of IHHNV,EHP and SHIV were 20.93%,31.78% and 37.21%,respectively. However,Tauras syndrome virus(TSV)was not detected from samples in the present research. SHIV、EHP and IHHNV were the main pathogens carried by shrimp seedlings in Zhejiang Province,which needed more attention. In terms of regional distribution,the samples collected from Ningbo City carried the most species of pathogens and the carrying rate was relatively high,indicating prevention and control should be strengthened by Ningbo Authorities. In terms of shrimp seed sources,the highest carrying rate(38.10%)of IHHNV was found in shrimp larvae from Fujian Province,WSSV infection was observed in shrimp larvae from Hainan Province and Guangdong Province,the detection rates were 2.17% and 2.27%,respectively. And the highest carrying rates of EHP and SHIV were both observed in shrimp larvae from Guangdong Province,which were 43.48% and 41.30%,respectively. Since the white prawn seedlings of Zhejiang Province are mainly imported from other provinces,where the infection rate is relatively high,hence the inspection and quarantine work towards such seedlings should be strengthened by Zhejiang Province.
In order to recognize the main pathogens carried by white shrimp(Penaeus vannamei)in Zhejiang Province,a total of 129 samples were collected from shrimp seed hatcheries in 4 cities of Hangzhou,Ningbo,Jiaxing and Shaoxin and used for pathogen detection. According to the results,four kinds of pathogens were detected,including white spot syndrome virus(WSSV),infectious hypodermal and hematopoietic necrosis virus(IHHNV),enterocytozoom hepatopenaei(EHP)and shrimp hemocyte iridescent virus(SHIV). The results showed that the positive rate of WSSV was 1.55%,while the rates of IHHNV,EHP and SHIV were 20.93%,31.78% and 37.21%,respectively. However,Tauras syndrome virus(TSV)was not detected from samples in the present research. SHIV、EHP and IHHNV were the main pathogens carried by shrimp seedlings in Zhejiang Province,which needed more attention. In terms of regional distribution,the samples collected from Ningbo City carried the most species of pathogens and the carrying rate was relatively high,indicating prevention and control should be strengthened by Ningbo Authorities. In terms of shrimp seed sources,the highest carrying rate(38.10%)of IHHNV was found in shrimp larvae from Fujian Province,WSSV infection was observed in shrimp larvae from Hainan Province and Guangdong Province,the detection rates were 2.17% and 2.27%,respectively. And the highest carrying rates of EHP and SHIV were both observed in shrimp larvae from Guangdong Province,which were 43.48% and 41.30%,respectively. Since the white prawn seedlings of Zhejiang Province are mainly imported from other provinces,where the infection rate is relatively high,hence the inspection and quarantine work towards such seedlings should be strengthened by Zhejiang Province.
引文
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[12]王筱珊,胡智博,费荣梅.江苏地区对虾3种病毒病的流行病学调查及5株IHHNV的编码区基因序列分析[J].水产学报,2017,41(10):1623-1630.
[13]YU X W,WANG J P,ZHANG W,et al.Prevalence of three shrimp viruses in Zhejiang Province in 2008[J].Virologica sinica,2011,26(1):67-71.
[14]林开,侯崇林,谢荣辉,等.三种对虾病毒在浙江省凡纳滨对虾中的流行性调查研究[J].水产科学,2013,32(3):161-164.
[15]施慧,谢建军,许文军,等.浙江地区凡纳滨对虾苗3种对虾病毒携带情况研究[J].浙江海洋学院学报(自然科学版),2012,23(2):25-30.
[16]农业部渔业渔政管理局,全国水产技术推广总站.2015年中国水生动物卫生状况报告[M].北京:中国农业出版社,2016.
[17]农业部渔业渔政管理局,全国水产技术推广总站.2016年中国水生动物卫生状况报告[M].北京:中国农业出版社,2017.
[18]张会军,王娟,雷燕.2015年南美白对虾病毒检测报告[J].当代水产,2016,41(2):86-87.
[19]BONAMI J R,TRUMPER B,MARI J,et al.Purification and characterization of the infectious hypodermal and haematopoietic necrosis virus of penaeid shrimps[J].The Journal of general virology,1990,71(11):2657-2664.
[20]童桂香,韦信贤,吴伟军,等.广西凡纳滨对虾IHHNV感染情况的调查与分析[J].南方农业学报,2013,44(12):2089-2093.
[21]叶键,许婷,施礼科,等.虾肝肠胞虫的研究进展[J].科学养鱼,2018(1):59-61.
[22]SANTHOSHKUUMAR S,SIVAKUMAR S,VIMAL S,et al.Biochemical changes and tissue distribution of Enterocytozoon hepatopenaei(EHP)in naturally and experimentally EHP-infected whiteleg shrimp,Litopenaeus vannamei(Boone,1931),in India[J].Journal of fish diseases,2017,40(4):529-539.
[23]FLEGEL T W.Historic emergence,impact and current status of shrimp pathogens in Asia[J].Journal of invertebrate pathology,2012,110(2):166-173.
[24]陈禄芝,余霞艳,胡一丞,等.粤西地区凡纳滨对虾虾肝肠胞虫、传染性皮下和造血组织坏死病毒感染情况的初步调查[J].渔业研究,2016(4):273-280
[25]李色东,陈禄芝,余霞艳.粤西白虾EHP和IHHNV流行性初步调查[J].海洋与渔业·水产前沿,2016,38(7):35-37.
[26]王博雅,王力,刘美如,等.凡纳滨对虾3种主要病毒和虾肝肠胞虫在辽宁地区的流行情况分析[J].大连海洋大学学报,2017,32(2):150-154.
[2]侯路红.对虾传染性皮下及造血组织坏死病毒(IHHNV)病毒样颗粒的研究[D].厦门:厦门大学,2009.
[3]夏小明.对虾WSSV和IHHNV病毒重组酶聚合酶扩增(RPA)快速检测方法的研究[D].上海:上海海洋大学,2015.
[4]农业部渔业渔政管理局.全国水产技术推广总站.水生动物防疫工作实用手册[M].北京:中国农业出版社,2015
[5]许杰,邓威,李军,等.虾肝肠胞虫(EHP)流行病学与检测技术研究进展[J].中国动物检疫,2018,35(2):64-68
[6]QIU L,CHEN M M,WANG R Y,et al.Complete genome sequence of shrimp hemocyte iridescent virus SHIV)isolated from white leg shrimp,Litopenaeus vannamei[J].Archives of virology,2018,163(3):781-785.
[7]QIU L,CHEN M M,WANG R Y,et al Characterization of a new member of Iridoviridae,Shrimp hemocyte iridescent virus(SHIV),found in white leg shrimp(Litopenaeus vannamei)[J].Scientific reports,2017(7):11834.
[8]国家质量监督检验检疫总局国家标准化管理委员会.对虾传染性皮下及造血组织坏死病毒(IHHNV)检测PCR法:GB/T 25878-2010[S].北京:中国标准出版社,2011.
[9]国家质量监督检验检疫总局国家标准化管理委员会.白斑综合征(WSD)诊断规程:GB/T 28630.2-2012[S].北京:中国标准出版社,2012.
[11]农业部.对虾桃拉综合症病毒诊断规程:SC/T7204.3-2007[S].北京:中国标准出版社,2007.
[10]JAROENLAK P,SANGUANRUT P,WILLIAMS B,et al.A nested PCR assay to avoid false positive detection of the microsporidian Enterocytozoon hepatopenaei(EHP)in environmental samples in shrimp farms[J].PLos one,2016,11(11):e0166320.
[12]王筱珊,胡智博,费荣梅.江苏地区对虾3种病毒病的流行病学调查及5株IHHNV的编码区基因序列分析[J].水产学报,2017,41(10):1623-1630.
[13]YU X W,WANG J P,ZHANG W,et al.Prevalence of three shrimp viruses in Zhejiang Province in 2008[J].Virologica sinica,2011,26(1):67-71.
[14]林开,侯崇林,谢荣辉,等.三种对虾病毒在浙江省凡纳滨对虾中的流行性调查研究[J].水产科学,2013,32(3):161-164.
[15]施慧,谢建军,许文军,等.浙江地区凡纳滨对虾苗3种对虾病毒携带情况研究[J].浙江海洋学院学报(自然科学版),2012,23(2):25-30.
[16]农业部渔业渔政管理局,全国水产技术推广总站.2015年中国水生动物卫生状况报告[M].北京:中国农业出版社,2016.
[17]农业部渔业渔政管理局,全国水产技术推广总站.2016年中国水生动物卫生状况报告[M].北京:中国农业出版社,2017.
[18]张会军,王娟,雷燕.2015年南美白对虾病毒检测报告[J].当代水产,2016,41(2):86-87.
[19]BONAMI J R,TRUMPER B,MARI J,et al.Purification and characterization of the infectious hypodermal and haematopoietic necrosis virus of penaeid shrimps[J].The Journal of general virology,1990,71(11):2657-2664.
[20]童桂香,韦信贤,吴伟军,等.广西凡纳滨对虾IHHNV感染情况的调查与分析[J].南方农业学报,2013,44(12):2089-2093.
[21]叶键,许婷,施礼科,等.虾肝肠胞虫的研究进展[J].科学养鱼,2018(1):59-61.
[22]SANTHOSHKUUMAR S,SIVAKUMAR S,VIMAL S,et al.Biochemical changes and tissue distribution of Enterocytozoon hepatopenaei(EHP)in naturally and experimentally EHP-infected whiteleg shrimp,Litopenaeus vannamei(Boone,1931),in India[J].Journal of fish diseases,2017,40(4):529-539.
[23]FLEGEL T W.Historic emergence,impact and current status of shrimp pathogens in Asia[J].Journal of invertebrate pathology,2012,110(2):166-173.
[24]陈禄芝,余霞艳,胡一丞,等.粤西地区凡纳滨对虾虾肝肠胞虫、传染性皮下和造血组织坏死病毒感染情况的初步调查[J].渔业研究,2016(4):273-280
[25]李色东,陈禄芝,余霞艳.粤西白虾EHP和IHHNV流行性初步调查[J].海洋与渔业·水产前沿,2016,38(7):35-37.
[26]王博雅,王力,刘美如,等.凡纳滨对虾3种主要病毒和虾肝肠胞虫在辽宁地区的流行情况分析[J].大连海洋大学学报,2017,32(2):150-154.