龙须藤茎皮甲醇提取物生物活性初探
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摘要
龙须藤Bauhinia championii在医用方面已证实具有良好的抗菌、消炎作用,但其在农用方面的活性研究还鲜有报道。本研究分别采用菌丝生长速率法和种子生物测定法,首次测定龙须藤茎皮甲醇提取物及分离组分对6种农业有害真菌和单、双子叶两种植物的生物活性。结果表明,龙须藤甲醇浸提物对水稻稻瘟病菌Magnaporthe oryzae有一定的抑制效果,对油菜Brassica napus和稗草Echinochloa crusgalli的根芽均没有抑制作用。但其甲醇浸提物中分离的大部分组分对供试病原菌和植物的根芽都表现出一定抑制作用。杀菌活性方面:10号组分对西瓜枯萎病菌Fusarium oxysporum和番茄早疫病菌Alternaria solani的抑制效果最好,抑制率分别达到80.0%和78.0%,除草活性方面:1～3号组分对油菜根的抑制率较高,达到50%以上,2号组分对稗草根的抑制效果最好,达到68%。可见,龙须藤茎皮提取物中含有具有良好杀菌活性和除草活性的物质。
Bauhinia championii has excellent anti-bacterial, anti-inflammatory and other medical effects, but few studies have been reported on its agricultural biological activities. In this study, mycelia growth rate and seed bioassay methods were used to determine the bioactivities of the methanol extracts and separated components from B. championii against six species of agricultural pathogenic fungi and two weeds. The results showed that the methanol extracts had moderate inhibitory activity against Magnaporthe oryzae, and had no inhibitory effects on the root and buds of Brassica napus and Echinochloa crusgalli. However, some of the components isolated from the methanol extracts showed moderate inhibition against the tested pathogens, and the roots and buds of tested plants. In particular, component 10 showed the best inhibitory effect against Fusarium oxysporum and Alternaria solani, with an inhibition rate of 80.0% and 78.0%, respectively. The components 1-3 showed more than 50% inhibition rate against the root of B. napus. The component 2 had the best inhibitory activity against E. crusgalli, reaching 68%. The results provided a new idea for the development of new botanical pesticides.
Bauhinia championii has excellent anti-bacterial, anti-inflammatory and other medical effects, but few studies have been reported on its agricultural biological activities. In this study, mycelia growth rate and seed bioassay methods were used to determine the bioactivities of the methanol extracts and separated components from B. championii against six species of agricultural pathogenic fungi and two weeds. The results showed that the methanol extracts had moderate inhibitory activity against Magnaporthe oryzae, and had no inhibitory effects on the root and buds of Brassica napus and Echinochloa crusgalli. However, some of the components isolated from the methanol extracts showed moderate inhibition against the tested pathogens, and the roots and buds of tested plants. In particular, component 10 showed the best inhibitory effect against Fusarium oxysporum and Alternaria solani, with an inhibition rate of 80.0% and 78.0%, respectively. The components 1-3 showed more than 50% inhibition rate against the root of B. napus. The component 2 had the best inhibitory activity against E. crusgalli, reaching 68%. The results provided a new idea for the development of new botanical pesticides.
引文
[1] 蔡亮亮.龙须藤多糖提取纯化及其对软骨细胞活性的影响[D].福州:福建中医药大学,2013.
[2] CHEN C C,CHEN Y P,HSU H Y,et al.New flavones from Bauhinia championii Benth [J].Chemical and Pharmaceutical Bulletin,1984,32(1):166-169.
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[4] 徐伟,郑海音,洪振丰,等.RP-HPLC测定龙须藤中4种黄酮类成分的含量[J].中国现代应用药学杂志,2009,26(9):763-766.
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[7] 叶蕻芝,郑春松,林薇,等.气相色谱质谱联用技术分析梅花入骨丹挥发油的化学成分[J].福建中医学院学报,2009,19(5):20-22.
[8] 易荆丽,张嘉家,周毅生,等.龙须藤提取物的镇痛抗炎作用[J].广东药学院学报,2012,28(6):647-651.
[9] 林丽微,周毅生,周臻,等.龙须藤总黄酮对膝关节炎模型大鼠的抗炎作用[J].广东药学院学报,2014,30(5):608-611.
[10] XU Wei,CHU Kedan,LI Huang,et al.Bauhinia championii extraction treatment of collagen-induced arthritis via downregulation of the expression of TLR4,MyD88 and NF-kB [J].American Journal of Chinese Medicine,2013,41(2):379-390.
[11] 高杰,林炜鑫,钟屿云,等.九龙藤乙酸乙酯提取物清除自由基、镇痛抗炎作用研究[J].安徽农业科学,2011,39(36):22305-22306.
[12] 林炜鑫.九龙藤总黄酮的提取纯化及抗心肌氧化损伤作用[D].桂林:桂林医学院,2013.
[13] 洪振丰,郑海音,徐伟,等.康美肤烧伤膏抗炎、镇痛作用的实验研究[J].中国中医药科技,2007,14(6):410-411.
[14] 叶蕻芝,陈加守,李西海,等.梅花入骨丹多糖对SW1353软骨肉瘤细胞活性的影响[J].福建中医药大学学报,2012,21(6):32-34.
[15] 江苏医学院.中药大辞典[M].上海:上海科学技术出版社,1986:13.
[16] 许桂芳,孟丽,刘荷芬,等.河南新乡太行山区野生植物资源及评价[J].河南科学,1999,17(6):121-123.
[17] 刘雪琴,周鸿燕.绿色农药研究进展[J].长江大学学报(自然科学版),2013,10(35):4-7.
[18] 姚宗理,吴清来,柯健,等.龙须藤叶粗提物的分离纯化及生物活性初探[J].广西植物,2018,38(7):886-893.
[19] 慕立义.植物化学保护研究方法[M].北京:中国农业出版社,1994:76-81.
[20] 许应生,吴培云,刘劲松,等.紫藤瘤不同溶剂提取部位抗农作物病原真菌活性研究[J].广西植物,2013,33(1):133-135.
[21] 徐江艳.稻田西来稗(Echinochloa crusgalli var.zelayensis)对二氯哇琳酸的抗药性及其机理研究[D].南京:南京农业大学,2013.
[2] CHEN C C,CHEN Y P,HSU H Y,et al.New flavones from Bauhinia championii Benth [J].Chemical and Pharmaceutical Bulletin,1984,32(1):166-169.
[3] 张玉琴.龙须藤抗类风湿关节炎的药效物质基础及作用机制研究[D].福州:福建中医药大学,2013.
[4] 徐伟,郑海音,洪振丰,等.RP-HPLC测定龙须藤中4种黄酮类成分的含量[J].中国现代应用药学杂志,2009,26(9):763-766.
[5] 白海云,詹庆丰,劳爱娜,等.九龙藤化学成分研究(Ⅰ)[J].中国中药杂志,2005,30(1):42-43.
[6] 汪艳娟,朱倩倩,叶蕻芝,等.微波提取龙须藤多糖的最佳条件研究[J].分析测试技术与仪器,2011,17(2):92-96.
[7] 叶蕻芝,郑春松,林薇,等.气相色谱质谱联用技术分析梅花入骨丹挥发油的化学成分[J].福建中医学院学报,2009,19(5):20-22.
[8] 易荆丽,张嘉家,周毅生,等.龙须藤提取物的镇痛抗炎作用[J].广东药学院学报,2012,28(6):647-651.
[9] 林丽微,周毅生,周臻,等.龙须藤总黄酮对膝关节炎模型大鼠的抗炎作用[J].广东药学院学报,2014,30(5):608-611.
[10] XU Wei,CHU Kedan,LI Huang,et al.Bauhinia championii extraction treatment of collagen-induced arthritis via downregulation of the expression of TLR4,MyD88 and NF-kB [J].American Journal of Chinese Medicine,2013,41(2):379-390.
[11] 高杰,林炜鑫,钟屿云,等.九龙藤乙酸乙酯提取物清除自由基、镇痛抗炎作用研究[J].安徽农业科学,2011,39(36):22305-22306.
[12] 林炜鑫.九龙藤总黄酮的提取纯化及抗心肌氧化损伤作用[D].桂林:桂林医学院,2013.
[13] 洪振丰,郑海音,徐伟,等.康美肤烧伤膏抗炎、镇痛作用的实验研究[J].中国中医药科技,2007,14(6):410-411.
[14] 叶蕻芝,陈加守,李西海,等.梅花入骨丹多糖对SW1353软骨肉瘤细胞活性的影响[J].福建中医药大学学报,2012,21(6):32-34.
[15] 江苏医学院.中药大辞典[M].上海:上海科学技术出版社,1986:13.
[16] 许桂芳,孟丽,刘荷芬,等.河南新乡太行山区野生植物资源及评价[J].河南科学,1999,17(6):121-123.
[17] 刘雪琴,周鸿燕.绿色农药研究进展[J].长江大学学报(自然科学版),2013,10(35):4-7.
[18] 姚宗理,吴清来,柯健,等.龙须藤叶粗提物的分离纯化及生物活性初探[J].广西植物,2018,38(7):886-893.
[19] 慕立义.植物化学保护研究方法[M].北京:中国农业出版社,1994:76-81.
[20] 许应生,吴培云,刘劲松,等.紫藤瘤不同溶剂提取部位抗农作物病原真菌活性研究[J].广西植物,2013,33(1):133-135.
[21] 徐江艳.稻田西来稗(Echinochloa crusgalli var.zelayensis)对二氯哇琳酸的抗药性及其机理研究[D].南京:南京农业大学,2013.