银鲫精母细胞型精原细胞瘤的病理学观察
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摘要
自银鲫(Carassius gibelio)中发现了一例自发性精母细胞型精原细胞瘤,详细描述了该肿瘤的组织病理和超微结构。在组织学上,该肿瘤具有薄膜包被、扩张性生长,具有高度细胞性和弥漫性结缔组织,伴有淋巴细胞浸润。透射电镜显示,该肿瘤显著的特征是含有未分化的精母细胞,且支撑细胞稀少。对比正常银鲫的精巢组织结构,排除了其他肿瘤类型,最终将该肿瘤鉴定为精母细胞型精原细胞瘤。系在低等脊椎动物中首次发现具有淋巴细胞浸润的精母细胞型精原细胞瘤,丰富了鱼类性腺肿瘤的基础资料,也为构建人类精母细胞型精原细胞瘤动物模型提供了依据。
This study described pathological features of a spontaneous spermatocytic seminoma in gibel carp Carassius gibelio. Histologically, the neoplasm founded in the abdomen was encapsulated invasive spermatocytic seminoma with an inconspicuous lobular architecture and diffuse fibro-connective septa with lymphocytic infiltration. For its ultrastructure, undifferentiated spermatocytes and fewer supporting cells were the unique features of the neoplasm. Compared with the histological features of normal testis of gibel carp, this neoplasm was identified as a spermatocytic seminoma.The first report of spermatocytic seminoma with lymphocytic infiltration in fish may contribute to the establishment of fish model of spermatocytic seminoma.
This study described pathological features of a spontaneous spermatocytic seminoma in gibel carp Carassius gibelio. Histologically, the neoplasm founded in the abdomen was encapsulated invasive spermatocytic seminoma with an inconspicuous lobular architecture and diffuse fibro-connective septa with lymphocytic infiltration. For its ultrastructure, undifferentiated spermatocytes and fewer supporting cells were the unique features of the neoplasm. Compared with the histological features of normal testis of gibel carp, this neoplasm was identified as a spermatocytic seminoma.The first report of spermatocytic seminoma with lymphocytic infiltration in fish may contribute to the establishment of fish model of spermatocytic seminoma.
引文
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[22]Li K B,Chang O Q,Shi C B,et al.Formation and pathological observation of melanoma of swordtail hybrids[J].Chinese Journal of Experimental Animals,2005,13(2):76-78[李凯彬,常藕琴,石存斌,等.剑尾鱼杂交种黑色素瘤的形成和病理观察.中国实验动物学报,2005,13(2):76-78]
[23]Jiang Q Q,Liu D E,Tian Z,et al.Biological characteristics and application of fish experimental animals[J].Journal of Hunan Ecological Science,2006,12(2):129-132[江千秋,刘冬娥,田智,等.鱼类实验动物的生物学特性及应用.湖南生态科学学报,2006,12(2):129-132]
[24]Wu D S,Wang Z B.The present situation and prospect of the development and application of fish experimental animals[J].Journal of Comparative Medicine in China,2000,10(2):103-109[吴端生,王宗保.鱼类实验动物开发与应用研究的现状及展望.中国比较医学杂志,2000,10(2):103-109]
[25]Pliss G B,Khudoley V V.Tumor induction by carcinogenic agents in aquarium fish[J].Journal of the National Cancer Institute,1975,55(1):129-136
[26]Chen Y H,Chen Z H,Xiao Z D.Fish Tumor[M].Guangdong Science and Technology Press.1993,107-108[陈玉汉,陈灼怀,肖振德.鱼类肿瘤.广东科技出版社.1993,107-108]
[27]Dickman M D,Steele P O.Gonadal neoplasms in wild carp-goldfish hybrids from the Welland River near Niagara Falls,Canada[J].Hydrobiologia,1986,134(3):257-263
[28]Leatherland J F,Sonstegard R A.Structure of normal testis and testicular tumors in cyprinids from Lake Ontario[J].Cancer Research,1978,38(10):3164
[29]Down N E,Leatherland J F.Histopathology of gonadal neoplasms in cyprinid fish from the lower Great Lakes of North America[J].Journal of Fish Diseases,1989,12(5):415-437
[2]Liu T H.Research progress of endocrine tumor histopathology and molecular pathology[J].Chinese Journal of Pathology,2004,33(3):193-194[刘彤华.内分泌肿瘤组织病理学及分子病理学研究进展.中华病理学杂志,2004,33(3):193-194]
[3]Groff J M.Neoplasia in fishes[J].Veterinary Clinics of North America Exotic Animal Practice,2004,7(3):705-756
[4]Harshbarger J C.Chronology of oncolygy in fish,amphibians and invertebrates[J].Bulletin-European Association of Fish Pathologists,2004,24(1):62-84
[5]Palikova M,Navratil S,Svobodova Z,et al.Skin and gonadal tumours in a barbel(Barbus barbus)-a case report[J].Bulletin-European Association of Fish Pathologists,2007,27(6):234-238
[6]Sirri R,Mandrioli L,Grieco V,et al.Seminoma in a koi carp Cyprinus carpio:histopathological and immunohistochemical findings[J].Diseases of Aquatic Organisms,2010,92(1):83-88
[7]Herman R L,Landolt M.A testicular leiomyoma in a largemouth bass,Micropterus salmoides[J].Journal of Wildlife Diseases,1975,11(1):128
[8]Blazer V S.Histopathological assessment of gonadal tissue in wild fishes[J].Fish Physiology&Biochemistry,2002,26(1):85-101
[9]Weisse C,Weber E S,Matzkin Z,et al.Surgical removal of a seminoma from a black sea bass[J].Journal of the American Veterinary Medical Association,2002,221(2):280
[10]Hubbard G B,Fletcher K C.A seminoma and a leiomyosarcoma in an Albino African Lungfish(Protopterus dolloi)[J].Journal of Wildlife Diseases,1985,21(1):72-74
[11]Masahito P,Ishikawa T,Takayama S.Spontaneous spermatocytic seminoma in African lungfish,Protopterus aethiopicus Heckel[J].Journal of Fish Diseases,2010,7(2):169-172
[12]Zhan M L,Li C Y,Gu P,et al.The clinicopathological features and immunophenotypic analysis[J].Anatomical Studies,2016,(6):437-440[詹玛琍,李传应,顾萍,等.睾丸精原细胞瘤临床病理特征与免疫表型分析.解剖学研究,2016,(6):437-440]
[13]Smolowitz R,Hanley J H.A three-year retrospective study of abdominal tumors in zebrafish maintained in an aquatic laboratory animal facility[J].Biological Bulletin,2002,203(2):265
[14]Basten S G,Davis E E,Gillis A J,et al.Mutations in LRRC50 predispose zebrafish and humans to seminomas[J].PLoS Genetics,2013,9(4):e1003384
[15]Spitsbergen J M,Kent M L.The state of the art of the zebrafish model for toxicology and toxicologic pathology research-advantages and current limitations[J].Toxicologic Pathology,2003,31Suppl(1):62
[16]Huang Can,Zhang Z H,Wu S Q,et al.The protective effect of probiotic Bacillus on the intestinal mucosal structure of Ctenopharyngodon indllus[J].Acta Hydrobiologica Sinica,2017,41(4):774-780[黄灿,张忠海,吴淑勤,等.益生芽孢杆菌对草鱼肠黏膜结构的保护作用.水生生物学报,2017,41(4):774-780]
[17]LüB J,Cheng J.New classification interpretation of the WHO testis and penile tumors in 2016[J].Chinese Journal of Pathology,2016,45(8):518-521[吕炳建,程亮.2016版WHO睾丸和阴茎肿瘤新分类解读.中华病理学杂志,2016,45(8):518-521]
[18]Nigrelli RF,Jakowska S.Spontaneous neoplasms in fishes,Vol 7.A spermatocytoma and renal melanoma in an African lungfish,Protopterus anneetens(Owen)[J].Zoologica,1953,38:109-112
[19]Hawkins W E,Fournie J W,Ishikawa T,et al.Germ cell neoplasms in Japanese Medaka[J].Journal of Aquatic Animal Health,1996,8(2):120-129
[20]Kennedy P C.Histological Classification of Tumors of the Genital System of Domestic Animals[M].Armed Forces Institute of Pathology.1998,17
[21]Han Q G,Liu S Z,Geng S S,et al.Pathological observation and analysis of spermatogenic cell tumor in dogs[J].Animal Husbandry and Veterinary Medicine,2017,49 (10):46-49[韩庆功,刘世忠,耿莎莎,等.犬睾丸精原细胞瘤病理学观察与分析.畜牧与兽医,2017,49(10):46-49]
[22]Li K B,Chang O Q,Shi C B,et al.Formation and pathological observation of melanoma of swordtail hybrids[J].Chinese Journal of Experimental Animals,2005,13(2):76-78[李凯彬,常藕琴,石存斌,等.剑尾鱼杂交种黑色素瘤的形成和病理观察.中国实验动物学报,2005,13(2):76-78]
[23]Jiang Q Q,Liu D E,Tian Z,et al.Biological characteristics and application of fish experimental animals[J].Journal of Hunan Ecological Science,2006,12(2):129-132[江千秋,刘冬娥,田智,等.鱼类实验动物的生物学特性及应用.湖南生态科学学报,2006,12(2):129-132]
[24]Wu D S,Wang Z B.The present situation and prospect of the development and application of fish experimental animals[J].Journal of Comparative Medicine in China,2000,10(2):103-109[吴端生,王宗保.鱼类实验动物开发与应用研究的现状及展望.中国比较医学杂志,2000,10(2):103-109]
[25]Pliss G B,Khudoley V V.Tumor induction by carcinogenic agents in aquarium fish[J].Journal of the National Cancer Institute,1975,55(1):129-136
[26]Chen Y H,Chen Z H,Xiao Z D.Fish Tumor[M].Guangdong Science and Technology Press.1993,107-108[陈玉汉,陈灼怀,肖振德.鱼类肿瘤.广东科技出版社.1993,107-108]
[27]Dickman M D,Steele P O.Gonadal neoplasms in wild carp-goldfish hybrids from the Welland River near Niagara Falls,Canada[J].Hydrobiologia,1986,134(3):257-263
[28]Leatherland J F,Sonstegard R A.Structure of normal testis and testicular tumors in cyprinids from Lake Ontario[J].Cancer Research,1978,38(10):3164
[29]Down N E,Leatherland J F.Histopathology of gonadal neoplasms in cyprinid fish from the lower Great Lakes of North America[J].Journal of Fish Diseases,1989,12(5):415-437