Anticancer potential of metabolic compounds from marine actinomycetes isolated from Lagos Lagoon sediment
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摘要
Thirty-two actinomycetes strains were isolated from sediment samples from 12 different sites at Lagos Lagoon and identified using standard physiological and biochemical procedures as well as 16 S rDNA gene sequence analysis. Secondary metabolites were extracted from the strains and their anticancer activity on the K562(Human acute myelocytic leukemia), HeLa(cervical carcinoma), AGS(Human gastric), MCF-7(breast adenocarcinoma) and HL-60(Human acute promyelocytic leukemia) cell lines was determined.The metabolic extracts exhibited cytotoxicity with IC50 values ranging from 0.030 mg/mL to 4.4 mg/mL.The Streptomyces bingchenggensis ULS14 extract was cytotoxic against all the cell lines tested. The bioactivity-guided extraction and purification of the metabolic extracts from this strain yielded two purified anticancer compounds: ULDF4 and ULDF5. The structures of the extracted compounds were determined using spectroscopic analyses, including electrospray ionization mass spectrophotometer and nuclear magnetic resonance(1 Dimensional and 2 Dimensional), and were shown to be structurally similar to staurosporine and kigamicin. The IC50 of ULDF4 and ULDF5 against the HeLa cell line was0.034 mg/mL and 0.075 mg/mL, respectively. This study is the first to reveal the anticancer potential of actinomycetes from Lagos Lagoon, which could be exploited for therapeutic purposes.
Thirty-two actinomycetes strains were isolated from sediment samples from 12 different sites at Lagos Lagoon and identified using standard physiological and biochemical procedures as well as 16 S rDNA gene sequence analysis. Secondary metabolites were extracted from the strains and their anticancer activity on the K562(Human acute myelocytic leukemia), HeLa(cervical carcinoma), AGS(Human gastric), MCF-7(breast adenocarcinoma) and HL-60(Human acute promyelocytic leukemia) cell lines was determined.The metabolic extracts exhibited cytotoxicity with IC50 values ranging from 0.030 mg/mL to 4.4 mg/mL.The Streptomyces bingchenggensis ULS14 extract was cytotoxic against all the cell lines tested. The bioactivity-guided extraction and purification of the metabolic extracts from this strain yielded two purified anticancer compounds: ULDF4 and ULDF5. The structures of the extracted compounds were determined using spectroscopic analyses, including electrospray ionization mass spectrophotometer and nuclear magnetic resonance(1 Dimensional and 2 Dimensional), and were shown to be structurally similar to staurosporine and kigamicin. The IC50 of ULDF4 and ULDF5 against the HeLa cell line was0.034 mg/mL and 0.075 mg/mL, respectively. This study is the first to reveal the anticancer potential of actinomycetes from Lagos Lagoon, which could be exploited for therapeutic purposes.
Thirty-two actinomycetes strains were isolated from sediment samples from 12 different sites at Lagos Lagoon and identified using standard physiological and biochemical procedures as well as 16 S rDNA gene sequence analysis. Secondary metabolites were extracted from the strains and their anticancer activity on the K562(Human acute myelocytic leukemia), HeLa(cervical carcinoma), AGS(Human gastric), MCF-7(breast adenocarcinoma) and HL-60(Human acute promyelocytic leukemia) cell lines was determined.The metabolic extracts exhibited cytotoxicity with IC50 values ranging from 0.030 mg/mL to 4.4 mg/mL.The Streptomyces bingchenggensis ULS14 extract was cytotoxic against all the cell lines tested. The bioactivity-guided extraction and purification of the metabolic extracts from this strain yielded two purified anticancer compounds: ULDF4 and ULDF5. The structures of the extracted compounds were determined using spectroscopic analyses, including electrospray ionization mass spectrophotometer and nuclear magnetic resonance(1 Dimensional and 2 Dimensional), and were shown to be structurally similar to staurosporine and kigamicin. The IC50 of ULDF4 and ULDF5 against the HeLa cell line was0.034 mg/mL and 0.075 mg/mL, respectively. This study is the first to reveal the anticancer potential of actinomycetes from Lagos Lagoon, which could be exploited for therapeutic purposes.
引文
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[14]S.Ravikumar,M.Gnanadesigan,N.Thajuddin,et al.,Anticancer property of sponge associated actinomycetes along Palk strait,J.Pharm.Res.3(2010)2415e2417.
[15]H.C.Kwon,C.A.Kauffman,P.R.Jensen,et al.,Marinisporolides,polyene-polyol macrolides from a marine actinomycete of the new genus“Marinispora”,J.Org.Chem.74(2009)675e684.
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[20]L.Malet-Cascon,F.Romero,F.Espliego-Vazquez,et al.,IB-00208,a new cytotoxic polycyclic xanthone produced by a marine-derived actinomadura,I.Isolation of the strain,taxonomy and biological activites,J.Antibiot(Tokyo)56(2003)219e225.
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[22]S.A.El-Shatoury,N.S.El-Shenawy,I.M.A.El-Salam,Antimicrobial,antitumor and in vivo cytotoxicity of actinomycetes inhabiting marine shellfish,World J.Microbiol.Biotechnol.25(2009)1547e1555.
[23]K.Hong,A.Gao,Q.Xie,et al.,Actinomycetes for marine drug discovery isolated from mangrove soils and plants in China,Mar.Drugs 7(2009)24e44.
[24]X.Gao,Y.Lu,Y.Xing,et al.,A novel anticancer and antifungus phenazine derivative from a marine actinomycete BM-17,Microbiol.Res.167(2012)616e622.
[25]K.Suthindhiran,K.Kannabiran,Cytotoxic and antimicrobial potential of actinomycete species Saccharopolyspora salina VITSDK4 isolated from the Bay of Bengal coast of India,Am.J.Infect.Dis.5(2009)90e98.
[26]S.Kunimoto,J.Lu,H.Esumi,et al.,Novel antitumor antibiotics I.Taxonomy,isolation,physico-chemical properties and biological activities,J.Antibiot(Tokyo)56(2003a)1004e1011.
[27]S.Kunimoto,T.Someno,Y.Yamazaki,et al.,Novel antitumor antibiotics.II.Structure determination,J.Antibiot(Tokyo)56(2003b)1012e1017.
[28]A.Y.G.Tan,S.Robinson,E.Lacey,et al.,Amycolatopsis regifaucium sp.nov.,a novel actinomycete that produces kigamicins,Int.J.Syst.Evol.Microbiol.57(2007)2562e2567.
[29]D.Dhanasekaran,N.Thajuddi,A.Panneerselvam,Antifungal compound:4'phenyl-1-napthyl ephenyl acetamide from Streptomyces sp.DPTB16,Med.Biol.15(2008)7e12.
[30]S.Omura,Y.Iwai,A.Hirano,et al.,A new alkaloid AM-2282 of Streptomyces origin taxonomy,fermentation,isolation and preliminary characterization,J.Antibiot(Tokyo)30(1977)275e282.
[31]H.J.Park,J.Y.Lee,I.S.Hwang,et al.,Isolation and antifungal and antioomycete activities of staurosporine from Streptomyces roseoflavus strain LS-A24,J.Agric.Food Chem.54(2006)3041e3046.
[32]H.Lovborg,P.Nygren,R.Larsson,Multiparametric evaluation of apoptosis:effects of standard cytotoxic agents and the cyanoguanidine CHS 828,Mol.Cancer Ther.3(2004)521e526.
[2]D.M.Parkin,J.Ferlay,M.Hamdi-Cherif,et al.,Cancer in Africad Epidemiology and Prevention,IARC Scientific Publications,Lyon,2003,pp.153.
[3]J.Ferlay,H.R.Shin,F.Bray,et al.,Estimates of worldwide burden of cancer in2008:GLOBOCAN 2008,Int.J.Cancer 127(2010)2893e2917.
[4]J.Berdy,Bioactive microbial metabolites,J.Antibiot(Tokyo)58(2005)1e26.
[5]C.Olano,C.Mendez,J.A.Salas,Antitumor compounds from actinomycetes:from gene clusters to new derivatives by combinatorial biosynthesis,Nat.Prod.Rep.26(2009a)628e660.
[6]C.Olano,C.Mendez,J.A.Salas,Antitumor compounds from marine actinomycetes,Mar.Drugs 7(2009b)210e248.
[7]D.J.Newman,G.M.Cragg,Natural products as sources of new drugs over the last 25 years,J.Nat.Prod.70(2007)461e477.
[8]A.L.Demain,S.Sanchez,Microbial drug discovery:80 years of progress,J.Antibiot(Tokyo)62(2009)5e16.
[9]O.F.Davies,I.A.Adeleye,P.G.Wang,Hyoscamine-producing marine actinomycetes from Lagos Lagoon sediment,Asian Pac.J.Trop.Biomed.3(2015)1e10.
[10]P.Elliah,T.Ramana,K.V.V.S.Bapi Raju,et al.,Investigation on marine actinomycetes from Bay of Bengal near Karnataka coast of Andhra Pradesh,Asian J.Microbiol.Biotechnol.Environ.Sci.6(2004)53e56.
[11]J.E.M.Stach,L.A.Maldonado,A.C.Ward,et al.,New primers for the class Actinobacteria:application to marine and terrestrial environments,Environ.Microbiol.5(2003)828e841.
[12]K.Tamura,D.Peterson,N.Peterson,et al.,MEGA5:molecular evolutionary genetics analysis using maximum likelihood,evolution distance and maximum parsimony methods,Mol.Biol.Evol.28(2011)2731e2739.
[13]A.Janardhan,A.P.Kumar,B.Viswanath,et al.,Production of bioactive compounds by actinomycetes and their antioxidant properties,Biotechnol.Res.Int.20(2014)1e8.
[14]S.Ravikumar,M.Gnanadesigan,N.Thajuddin,et al.,Anticancer property of sponge associated actinomycetes along Palk strait,J.Pharm.Res.3(2010)2415e2417.
[15]H.C.Kwon,C.A.Kauffman,P.R.Jensen,et al.,Marinisporolides,polyene-polyol macrolides from a marine actinomycete of the new genus“Marinispora”,J.Org.Chem.74(2009)675e684.
[16]E.A.Lewis,M.Munde,S.Wang,et al.,Complexity in the binding of minor groove agents:netropsin has two thermodynamically different DNA binding modes at a single site,Nucleic Acids Res.39(2011)9649e9658.
[17]R.Liu,T.Zhu,D.Li,et al.,Two indolocarbazole alkaloids with apoptosis activity from a marine-derived actinomycete Z2039-2,Arch Pharm.Res.30(2007)270e274.
[18]K.Saurav,K.Kannabiran,Cytotoxicity and antioxidant activity of 5-(2,4-dimethylbenzyl)pyrrolidin-2-one extracted from marine Streptomyces VITSVK5 spp,Saudi J.Biol.Sci.19(2012)81e86.
[19]M.A.Moran,L.T.Rutherford,R.E.Hodson,Evidence for indigenous Streptomyces populations in a marine environment determined with a 16S rRNAprobe,Appl.Environ.Microbiol.61(1995)3695e3700.
[20]L.Malet-Cascon,F.Romero,F.Espliego-Vazquez,et al.,IB-00208,a new cytotoxic polycyclic xanthone produced by a marine-derived actinomadura,I.Isolation of the strain,taxonomy and biological activites,J.Antibiot(Tokyo)56(2003)219e225.
[21]A.Becerril-Espinosa,G.Guerra-Rivas,N.Ayala-Sanchez,et al.,Antitumor activity of actinobacteria isolated in marine sediment from Todos Santos Bay,Baja California,Mexico,Rev.Biol.Mar.Oceanogr.47(2012)317e325.
[22]S.A.El-Shatoury,N.S.El-Shenawy,I.M.A.El-Salam,Antimicrobial,antitumor and in vivo cytotoxicity of actinomycetes inhabiting marine shellfish,World J.Microbiol.Biotechnol.25(2009)1547e1555.
[23]K.Hong,A.Gao,Q.Xie,et al.,Actinomycetes for marine drug discovery isolated from mangrove soils and plants in China,Mar.Drugs 7(2009)24e44.
[24]X.Gao,Y.Lu,Y.Xing,et al.,A novel anticancer and antifungus phenazine derivative from a marine actinomycete BM-17,Microbiol.Res.167(2012)616e622.
[25]K.Suthindhiran,K.Kannabiran,Cytotoxic and antimicrobial potential of actinomycete species Saccharopolyspora salina VITSDK4 isolated from the Bay of Bengal coast of India,Am.J.Infect.Dis.5(2009)90e98.
[26]S.Kunimoto,J.Lu,H.Esumi,et al.,Novel antitumor antibiotics I.Taxonomy,isolation,physico-chemical properties and biological activities,J.Antibiot(Tokyo)56(2003a)1004e1011.
[27]S.Kunimoto,T.Someno,Y.Yamazaki,et al.,Novel antitumor antibiotics.II.Structure determination,J.Antibiot(Tokyo)56(2003b)1012e1017.
[28]A.Y.G.Tan,S.Robinson,E.Lacey,et al.,Amycolatopsis regifaucium sp.nov.,a novel actinomycete that produces kigamicins,Int.J.Syst.Evol.Microbiol.57(2007)2562e2567.
[29]D.Dhanasekaran,N.Thajuddi,A.Panneerselvam,Antifungal compound:4'phenyl-1-napthyl ephenyl acetamide from Streptomyces sp.DPTB16,Med.Biol.15(2008)7e12.
[30]S.Omura,Y.Iwai,A.Hirano,et al.,A new alkaloid AM-2282 of Streptomyces origin taxonomy,fermentation,isolation and preliminary characterization,J.Antibiot(Tokyo)30(1977)275e282.
[31]H.J.Park,J.Y.Lee,I.S.Hwang,et al.,Isolation and antifungal and antioomycete activities of staurosporine from Streptomyces roseoflavus strain LS-A24,J.Agric.Food Chem.54(2006)3041e3046.
[32]H.Lovborg,P.Nygren,R.Larsson,Multiparametric evaluation of apoptosis:effects of standard cytotoxic agents and the cyanoguanidine CHS 828,Mol.Cancer Ther.3(2004)521e526.