法医体液溯源的分子生物学技术研究进展
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摘要
通过对犯罪现场发现的体液溯源,可以在样本来源者与实际犯罪行为之间建立联系,为现场重建提供重要证据。随着分子生物学技术的发展,DNA甲基化、RNA等遗传标记以及特定微生物检测被发现具有潜在的法医体液溯源价值。本文对近年来应用分子生物学方法进行法医体液溯源的研究现状和应用前景进行综述。
Determination of the type and origin of the body fluids found at a crime scene is important to the reconstruction of crime scene by building a link between sample donors and actual criminal acts. With the development of molecular biology, genetic makers such as DNA methylation and RNA profiling, and specific microbial detection have been found to have potential value for body fluid identification. In this paper, we provide an overview of the research status and application prospects of forensic body fluid identification using molecular biology methods in recent years.
Determination of the type and origin of the body fluids found at a crime scene is important to the reconstruction of crime scene by building a link between sample donors and actual criminal acts. With the development of molecular biology, genetic makers such as DNA methylation and RNA profiling, and specific microbial detection have been found to have potential value for body fluid identification. In this paper, we provide an overview of the research status and application prospects of forensic body fluid identification using molecular biology methods in recent years.
引文
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[14]Hanson EK,Lubenow H,Ballantyne J.Identification of forensically relevant body fluids using a panel of differentially expressed microRNAs[J].Anal Biochem,2009,387(2):303-314.
[15]Zubakov D,Boersma AWM,Ying C,et al.MicroRNAmarkers for forensic body fluid identification obtained from microarray screening and quantitative RT-PCRconfirmation[J].Int J Legal Med,2010,124(3):217-226.
[16]Wang Z,Zhang J,Luo H,et al.Screening and confirmation of micro RNA markers for forensic body fluid identification[J].Forensic Sci Int Genet,2013,7(1):116-123.
[17]Wang Z,Zhou D,Cao Y,et al.Characterization of microRNA expression profiles in blood and saliva using the Ion Personal Genome Machine(?)System(Ion PGMTM System)[J].Forensic Sci Int Genet,2016,20:140-146.
[18]Tian H,Lv M,Li Z,et al.Semen-specific miRNAs:Suitable for the distinction of infertile semen in the body fluid identification?[J].Forensic Sci Int Genet,2018,33:161-167.
[19]Dong WW,Li HM,Qing XR,et al.Identification and characteriza-tion of human testis derived circular RNAs and their existence in seminal plasma[J].Sci Rep,2016,6:39080.
[20]Bahn JH,Zhang Q,Li F,et al.The landscape of microRNA,Piwi-interacting RNA,and circular RNA in human saliva[J].Clin Chem,2015,61(1):221-230.
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[22]Song F,Luo HB,Xie MK,et al.Microarray expression profile of circular RNAs in human body fluids[J].Forensic Sci Int Genet Suppl Ser,2017,6:e55-e56.
[23]Zhang YQ,Liu BN,Shao CC,et al.Evaluation of the inclusion of circular RNAs in mRNA profiling in forensic body fluid identification[J].Int J Legal Med,2018,132(1):43-52.
[24]Giampaoli S,Berti A,Valeriani F,et al.Molecular identification of vaginal fluid by microbial signature[J].Forensic Sci Int Genet,2012,6(5):559-564.
[25]Zou KN,Ren LJ,Ping Y,et al.Identification of vaginal fluid,saliva,and feces using microbial signatures in a Han Chinese population[J].J Forensic Leg Med,2016,43:126-131.
[2]Dan F,Wasserstrom A,Budowle B,et al.DNAmethylation-based forensic tissue identification[J].Forensic Sci Int Genet,2011,5(5):517-524.
[3]Park J L,Kwon O H,Kim J H,et al.Identification of body fluid-specific DNA methylation markers for use in forensic science[J].Forensic Sci Int Genet,2014,13(8):147-153.
[4]Lee HY,An JH,Jung SE,et al.Genome-wide methylation profiling and a multiplex construction for the identification of body fluids using epigenetic markers[J].Forensic Sci Int Genet,2015,17:17-24.
[5]Lee HY,Jung SE,Lee EH,et al.DNA methylation profiling for a confirmatory test for blood,saliva,semen,vaginal fluid and menstrual blood[J].Forensic Sci Int Genet,2016,24:75-82.
[6]Zubakov D,Hanekamp E,Kokshoorn M,et al.Stable RNA markers for identification of blood and saliva stains revealed from whole genome expression analysis of timewise degraded samples[J].Int J Legal Med,2008,122(2):135-142.
[7]Wang Z,Zhang SH,Di Z,et al.Messenger RNA Profiling for Forensic Body Fluid Identification:Research and Applications[J].J Forensic Sci Med,2013,29(5):368-374.
[8]Albani PP,Fleming R.Novel messenger RNAs for body fluid identification[J].Sci Justice,2018,58(2):145-152.
[9]Zubakov D,Kokmeijer I,Ralf A,et al.Towards simultaneous individual and tissue identification:Aproof-of-principle study on parallel sequencing of STRs,amelogenin,and mRNAs with the Ion Torrent PGM[J].Forensic Sci Int Genet,2015,17:122-128.
[10]Hanson E,Ingold S,Haas C,et al.Messenger RNAbiomarker signatures for forensic body fluid identification revealed by targeted RNA sequencing[J].Forensic Sci Int Genet,2018,34:206-221.
[11]D?rum G,Ingold S,Hanson E,et al.Predicting the origin of stains from next generation sequencing mRNA data[J].Forensic Sci Int Genet,2018,34:37-48.
[12]Ingold S,Dorum G,Hanson E,et al.Body fluid identification using a targeted mRNA massively parallel sequencing approach-results of a Euroforgen/Ednap collaborative exercise[J].Forensic Sci Int Genet,2018,34:105-115.
[13]Zhao H,Wang C,Yao L,et al.Identification of aged bloodstains through mRNA profiling:Experiments results on selected markers of 30-and 50-year-old samples[J].Forensic Sci Int,2017,272:e1-e6.
[14]Hanson EK,Lubenow H,Ballantyne J.Identification of forensically relevant body fluids using a panel of differentially expressed microRNAs[J].Anal Biochem,2009,387(2):303-314.
[15]Zubakov D,Boersma AWM,Ying C,et al.MicroRNAmarkers for forensic body fluid identification obtained from microarray screening and quantitative RT-PCRconfirmation[J].Int J Legal Med,2010,124(3):217-226.
[16]Wang Z,Zhang J,Luo H,et al.Screening and confirmation of micro RNA markers for forensic body fluid identification[J].Forensic Sci Int Genet,2013,7(1):116-123.
[17]Wang Z,Zhou D,Cao Y,et al.Characterization of microRNA expression profiles in blood and saliva using the Ion Personal Genome Machine(?)System(Ion PGMTM System)[J].Forensic Sci Int Genet,2016,20:140-146.
[18]Tian H,Lv M,Li Z,et al.Semen-specific miRNAs:Suitable for the distinction of infertile semen in the body fluid identification?[J].Forensic Sci Int Genet,2018,33:161-167.
[19]Dong WW,Li HM,Qing XR,et al.Identification and characteriza-tion of human testis derived circular RNAs and their existence in seminal plasma[J].Sci Rep,2016,6:39080.
[20]Bahn JH,Zhang Q,Li F,et al.The landscape of microRNA,Piwi-interacting RNA,and circular RNA in human saliva[J].Clin Chem,2015,61(1):221-230.
[21]Xia SY,Feng J,Lei LJ,et al.Comprehensive characterization of tissue-specific circular RNAs in the human and mouse genomes[J].Brief Bioinform,2016,18(6):984-992.
[22]Song F,Luo HB,Xie MK,et al.Microarray expression profile of circular RNAs in human body fluids[J].Forensic Sci Int Genet Suppl Ser,2017,6:e55-e56.
[23]Zhang YQ,Liu BN,Shao CC,et al.Evaluation of the inclusion of circular RNAs in mRNA profiling in forensic body fluid identification[J].Int J Legal Med,2018,132(1):43-52.
[24]Giampaoli S,Berti A,Valeriani F,et al.Molecular identification of vaginal fluid by microbial signature[J].Forensic Sci Int Genet,2012,6(5):559-564.
[25]Zou KN,Ren LJ,Ping Y,et al.Identification of vaginal fluid,saliva,and feces using microbial signatures in a Han Chinese population[J].J Forensic Leg Med,2016,43:126-131.