葡萄糖、丙三醇和盐对厚唇裂腹鱼精子活力的调控研究
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摘要
为了探究厚唇裂腹鱼(Schizothorax irregularis)精子活力的调控规律,提高受精率和苗种生产效率,文章采用显著性检验和响应面法,研究了葡萄糖、丙三醇和3种盐对厚唇裂腹鱼精子活力的单项调控和复合项调控作用。结果表明,葡萄糖为433.7 kPa时厚唇裂腹鱼精子的快速运动时间(FT)最大(37.00 s),显著高于其他组(P<0.05);丙三醇为506.7 kPa时厚唇裂腹鱼精子的FT最大(32.33 s),与416.3 kPa组无差异,但显著高于其他组(P<0.05);氯化钠为431.4 kPa时厚唇裂腹鱼精子的FT最大(35.00 s),与348.8 kPa组无差异,但显著高于其他组(P<0.05);氯化钾对精子的激活效果不明显,氯化镁则有抑制效果;建立了厚唇裂腹鱼精子FT与葡萄糖、丙三醇和氯化钠的拟合方程,该方程的一次效应、二次效应均极显著(P<0.01),交互效应显著(P<0.05);模型优化配方为氯化钠164.78 kPa、丙三醇216.55 kPa、葡萄糖208.43 kPa,理论FT为68.24 s。运用响应面法优化的激活液配方,能有效提高该鱼的精子活力。
In order to explore the regulation rule of spermatozoa motility of Schizothorax irregularis, and to improve its fertilization rate and fingerling production efficiency, we applied a significance test and response surface method to study the single regulation and compound regulation of glucose, glycerol and three salts on sperm motility of S. irregularis. The results show that the maximum fast movement time of S. irregularis spermatozoa was 37.00 s when glucose was 433.7 kPa, significantly higher than those in the other groups(P<0.05). The maximum fast movement time of S. irregularis spermatozoa was 32.33 s when glycerol was 506.7 k Pa,without difference with that in 416.3 kPa group, but was significantly higher than those in the other groups(P<0.05). The maximum fast movement time of S. irregularis spermatozoa was 35.00 s when sodium chloride was 431.4 kPa, without difference with that in348.8 kPa group, but was significantly higher than those in the other groups(P<0.05). Potassium chloride had no obvious effect on activating spermatozoa, and magnesium chloride solution showed inhibition effect for spermatozoa. An equation about the relation of fast movement time and glucose, glycerol and sodium chloride was established. The linear and quadratic effects of the equation were very significant(P<0.01), and interaction effect was significant(P<0.05). The optimizied formulation of model was sodium chloride of 164.78 kPa, glycerol of 216.55 kPa, glucose of 208.43 kPa, and the theoretical FT was 68.24 s. The results indicate that the optimized activator formula by response surface methodology can improve the motility of S. irregularis spermatozoa.
In order to explore the regulation rule of spermatozoa motility of Schizothorax irregularis, and to improve its fertilization rate and fingerling production efficiency, we applied a significance test and response surface method to study the single regulation and compound regulation of glucose, glycerol and three salts on sperm motility of S. irregularis. The results show that the maximum fast movement time of S. irregularis spermatozoa was 37.00 s when glucose was 433.7 kPa, significantly higher than those in the other groups(P<0.05). The maximum fast movement time of S. irregularis spermatozoa was 32.33 s when glycerol was 506.7 k Pa,without difference with that in 416.3 kPa group, but was significantly higher than those in the other groups(P<0.05). The maximum fast movement time of S. irregularis spermatozoa was 35.00 s when sodium chloride was 431.4 kPa, without difference with that in348.8 kPa group, but was significantly higher than those in the other groups(P<0.05). Potassium chloride had no obvious effect on activating spermatozoa, and magnesium chloride solution showed inhibition effect for spermatozoa. An equation about the relation of fast movement time and glucose, glycerol and sodium chloride was established. The linear and quadratic effects of the equation were very significant(P<0.01), and interaction effect was significant(P<0.05). The optimizied formulation of model was sodium chloride of 164.78 kPa, glycerol of 216.55 kPa, glucose of 208.43 kPa, and the theoretical FT was 68.24 s. The results indicate that the optimized activator formula by response surface methodology can improve the motility of S. irregularis spermatozoa.
引文
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[6]刘鉴毅,甘芳,危起伟,等.几种不同浓度的离子及单糖对中华鲟精子活力的影响[J].水生生物学报,2007(6):849-854.
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[8]甘芳,杜浩,危起伟,等.Na+、K+、Mg2+、Ca2+和葡萄糖溶液作为授精-激活介质对中华鲟精子受精率的影响[J].动物学杂志,2009,44(1):106-111.
[9]宋勇,陈生熬,梁海波,等.NaCl、KCl和葡萄糖对叶尔羌高原鳅精子活力的影响[J].科学养鱼,2018(2):54-56.
[10]阮国良,冯家斌,杨代勤.渗透压、pH和温度对泥鳅精子活力及受精率的影响[J].湖北农学院学报,2004,24(1):22-25.
[11]区又君,廖光勇,陈超,等.七带石斑鱼精子活力及其与环境的关系[J].海洋环境科学,2011,30(4):516-519.
[12]魏平,叶霆,吴向丹,等.环境因子对眼斑拟石首鱼精子活力的影响[J].生态科学,2009,28(5):438-442.
[13]顾正选,丁诗华.pH值及不同百分浓度NaCl溶液对齐口裂腹鱼精子活力的影响[J].西南大学学报(自然科学版),2017,39(7):72-76.
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[16]张人铭,郭焱,马燕武,等.塔里木裂腹鱼资源与分布的调查研究[J].淡水渔业,2007,37(6):76-78.
[17]费骥慧,汪兴中,邵晓阳.洱海鱼类群落的空间分布格局[J].水产学报,2012,36(8):1225-1233.
[18]海萨·艾也力汗,郭焱,孟玮,等.新疆裂腹鱼类的系统发生关系及物种分化时间[J].遗传,2014,36(10):1013-1020.
[19]代应贵,肖海.裂腹鱼类种质多样性研究综述[J].中国农学通报,2011,27(32):38-46.
[20]马燕武,郭焱,张人铭,等.新疆塔里木河水系土著鱼类区系组成与分布[J].水产学报,2009,33(6):949-956.
[21]叶尚明,李兴玖,李晓春,等.新疆塔什库尔干河水库建设对鱼类资源的影响[J].水利渔业,2004,24(4):63-65.
[22]CHEN S A,GONG Y R,WANG M,et al.Length-weight relationship of two fish species in Weigan River[J].Anim Husb Feed Sci,2015,36(8):5-10.
[23]杨天燕,孟玮,郭焱,等.新疆两种裂腹鱼形态学和COI基因的比较分析[J].水产学杂志,2013,26(1):10-14.
[24]苏德学,严安生,田永胜,等.阳离子,葡萄糖及渗透压对丁鱥精子活力的影响[J].水利渔业,2004,24(1):7-8.
[25]胡一中+.Na+、K+、Ca2、葡萄糖及渗透压对泥鳅精子活力的影响[J].金华职业技术学院学报,2010,10(3):69-72.
[26]罗明坤,赵岩,JEERAWAT T,等.pH、盐度及K+、Ca2+和葡萄糖对萨罗罗非鱼精子活力的影响[J].南方农业学报,2014,45(11):2076-2080.
[27]丁淑荃,李飞,万全,等.不同浓度葡萄糖、NaCl和KCl对蛇精子活力的影响[J].安徽农业大学学报,2012,39(6):881-884.
[28]金穗华,王鹏武,马国辉,等.稀释液不同甘油浓度对公牛精子活力及相关酶渗出量的影响[J].中国奶牛,2016(11):17-21.
[29]TAKEI G L,MUKAI C,OKUNO M.Regulation of salmonid fish sperm motility by osmotic shock-induced water influx across the plasma membrane[J].Comp Biochem Physiol A,2015,182:84-92.
[30]TAKEI G L,MUKAI C,OKUNO M.Transient Ca2+mobilization caused by osmotic shock initiates salmonid fish sperm motility[J].J Exp Biol,2012,215(Pt 4):630-641.
[31]张涛,李胜忠,牛建功,等.Na+、K+、葡萄糖及甘油对高体雅罗鱼精子活力的影响[J].南方农业学报,2017,48(4):734-738.
[32]李胜忠,张健,成沛,等.甘露醇和甘油对白斑狗鱼精子活力的影响[J].水产养殖,2013,34(1):18-20.
[33]PROKOPCHUK G,DZYUBA B,RODINA M,et al.Control of sturgeon sperm motility:antagonism between K+ions concentration and osmolality[J].Anim Reprod Sci,2016,164:82-89.
[34]苏德学,严安生,田永胜,等.钠、钾、钙和葡萄糖对白斑狗鱼精子活力的影响[J].动物学杂志,2004,39(1):16-20.
[35]吴清毅,孙翰昌,李云瑶.不同浓度的K+、Ca2+和葡萄糖对乌原鲤精子活力的影响[J].水产科学,2011,30(4):202-205.
[36]王芳,岳兴建,谢碧文,等.pH、葡萄糖以及金属离子对翘嘴红鲌精子活力的影响[J].安徽农业科学,2011,39(27):16812-16884.
[37]闫秀明,张小雪.响应面分析法优化黄鳝精子激活条件[J].水产科技情报,2010,37(6):269-273.
[38]ARPI N,FAHRIZAL,NOVITA M.Isolation of fish skin and bone gelatin from tilapia(Oreochromis niloticus):response surface approach[J].IOP Conf Ser,2018,334(1):12-61.
[39]ALI J M,D'SOUZA D L,SCHWARZ K,et al.Response and recovery of fathead minnows(Pimephales promelas)following early life exposure to water and sediment found within agricultural runoff from the Elkhorn River,Nebraska,USA[J].Sci Total Environ,2018,618:1371-1381.
[40]ADAMEK M,HAZERLI D,MATRAS M,et al.Viral infections in common carp lead to a disturbance of mucin expression in mucosal tissues[J].Fish Shellfish Immunol,2017,71:353-358.
[41]黄建盛,陈刚,张健东,等.基于响应面法分析温度和盐度对虎斑乌贼受精卵孵化的联合影响[J].生态学杂志,2016,35(2):446-452.
[42]赵强,余四九,王廷璞,等.响应面法优化秃疮花中生物碱提取工艺及抑菌活性研究[J].草业学报,2012,21(4):206-214.
[43]钱佳慧,栗志民,申玉春,等.温度和盐度对华贵栉孔扇贝抗氧化酶活性的联合效应研究[J].南方水产科学,2015,11(6):49-57.
[44]黄建盛,陈刚,张健东,等.响应曲面法分析低盐条件下温度与pH对斜带石斑鱼幼鱼能量代谢的联合效应[J].海洋渔业,2017,39(1):41-50.
[2]韩英,张澜澜,徐革峰,等.黑龙江茴鱼(Thymallus arcticus grubei Dybowski)精子活力的观察[J].水产学杂志,2008,21(1):9-14.
[3]王贵英,李清,祝东梅,等.黑尾近红鲌精子活力研究[J].湖北农业科学,2016,55(8):2062-2065.
[4]DUMORNE K,FIGUEROA E,COSSON J,et al.Protein phosphorylation and ions effects on salmonid sperm motility activation[J].Rev Aquacult,2018,10(3):727-737.
[5]LE HOANG M,PHAM H Q.Sperm motilities in Waigieu seaperch,Psammoperca waigiensis:effects of various dilutions,pH,temperature,osmolality,and cations[J].J World Aquacult Soc,2017,48(3):435-443.
[6]刘鉴毅,甘芳,危起伟,等.几种不同浓度的离子及单糖对中华鲟精子活力的影响[J].水生生物学报,2007(6):849-854.
[7]周定刚,温安祥.黄鳝精子活力检测和精子入卵早期过程观察[J].水产学报,2003,27(5):398-402.
[8]甘芳,杜浩,危起伟,等.Na+、K+、Mg2+、Ca2+和葡萄糖溶液作为授精-激活介质对中华鲟精子受精率的影响[J].动物学杂志,2009,44(1):106-111.
[9]宋勇,陈生熬,梁海波,等.NaCl、KCl和葡萄糖对叶尔羌高原鳅精子活力的影响[J].科学养鱼,2018(2):54-56.
[10]阮国良,冯家斌,杨代勤.渗透压、pH和温度对泥鳅精子活力及受精率的影响[J].湖北农学院学报,2004,24(1):22-25.
[11]区又君,廖光勇,陈超,等.七带石斑鱼精子活力及其与环境的关系[J].海洋环境科学,2011,30(4):516-519.
[12]魏平,叶霆,吴向丹,等.环境因子对眼斑拟石首鱼精子活力的影响[J].生态科学,2009,28(5):438-442.
[13]顾正选,丁诗华.pH值及不同百分浓度NaCl溶液对齐口裂腹鱼精子活力的影响[J].西南大学学报(自然科学版),2017,39(7):72-76.
[14]郭焱,张人铭,蔡林钢.新疆鱼类志[M].乌鲁木齐:新疆科学技术出版社,2012:106-118.
[15]任慕莲,任波,杨植林,等.濒危物种--新疆大头鱼的生态和资源调查与保护对策的研究[J].水产学杂志,1996,9(2):1-19.
[16]张人铭,郭焱,马燕武,等.塔里木裂腹鱼资源与分布的调查研究[J].淡水渔业,2007,37(6):76-78.
[17]费骥慧,汪兴中,邵晓阳.洱海鱼类群落的空间分布格局[J].水产学报,2012,36(8):1225-1233.
[18]海萨·艾也力汗,郭焱,孟玮,等.新疆裂腹鱼类的系统发生关系及物种分化时间[J].遗传,2014,36(10):1013-1020.
[19]代应贵,肖海.裂腹鱼类种质多样性研究综述[J].中国农学通报,2011,27(32):38-46.
[20]马燕武,郭焱,张人铭,等.新疆塔里木河水系土著鱼类区系组成与分布[J].水产学报,2009,33(6):949-956.
[21]叶尚明,李兴玖,李晓春,等.新疆塔什库尔干河水库建设对鱼类资源的影响[J].水利渔业,2004,24(4):63-65.
[22]CHEN S A,GONG Y R,WANG M,et al.Length-weight relationship of two fish species in Weigan River[J].Anim Husb Feed Sci,2015,36(8):5-10.
[23]杨天燕,孟玮,郭焱,等.新疆两种裂腹鱼形态学和COI基因的比较分析[J].水产学杂志,2013,26(1):10-14.
[24]苏德学,严安生,田永胜,等.阳离子,葡萄糖及渗透压对丁鱥精子活力的影响[J].水利渔业,2004,24(1):7-8.
[25]胡一中+.Na+、K+、Ca2、葡萄糖及渗透压对泥鳅精子活力的影响[J].金华职业技术学院学报,2010,10(3):69-72.
[26]罗明坤,赵岩,JEERAWAT T,等.pH、盐度及K+、Ca2+和葡萄糖对萨罗罗非鱼精子活力的影响[J].南方农业学报,2014,45(11):2076-2080.
[27]丁淑荃,李飞,万全,等.不同浓度葡萄糖、NaCl和KCl对蛇精子活力的影响[J].安徽农业大学学报,2012,39(6):881-884.
[28]金穗华,王鹏武,马国辉,等.稀释液不同甘油浓度对公牛精子活力及相关酶渗出量的影响[J].中国奶牛,2016(11):17-21.
[29]TAKEI G L,MUKAI C,OKUNO M.Regulation of salmonid fish sperm motility by osmotic shock-induced water influx across the plasma membrane[J].Comp Biochem Physiol A,2015,182:84-92.
[30]TAKEI G L,MUKAI C,OKUNO M.Transient Ca2+mobilization caused by osmotic shock initiates salmonid fish sperm motility[J].J Exp Biol,2012,215(Pt 4):630-641.
[31]张涛,李胜忠,牛建功,等.Na+、K+、葡萄糖及甘油对高体雅罗鱼精子活力的影响[J].南方农业学报,2017,48(4):734-738.
[32]李胜忠,张健,成沛,等.甘露醇和甘油对白斑狗鱼精子活力的影响[J].水产养殖,2013,34(1):18-20.
[33]PROKOPCHUK G,DZYUBA B,RODINA M,et al.Control of sturgeon sperm motility:antagonism between K+ions concentration and osmolality[J].Anim Reprod Sci,2016,164:82-89.
[34]苏德学,严安生,田永胜,等.钠、钾、钙和葡萄糖对白斑狗鱼精子活力的影响[J].动物学杂志,2004,39(1):16-20.
[35]吴清毅,孙翰昌,李云瑶.不同浓度的K+、Ca2+和葡萄糖对乌原鲤精子活力的影响[J].水产科学,2011,30(4):202-205.
[36]王芳,岳兴建,谢碧文,等.pH、葡萄糖以及金属离子对翘嘴红鲌精子活力的影响[J].安徽农业科学,2011,39(27):16812-16884.
[37]闫秀明,张小雪.响应面分析法优化黄鳝精子激活条件[J].水产科技情报,2010,37(6):269-273.
[38]ARPI N,FAHRIZAL,NOVITA M.Isolation of fish skin and bone gelatin from tilapia(Oreochromis niloticus):response surface approach[J].IOP Conf Ser,2018,334(1):12-61.
[39]ALI J M,D'SOUZA D L,SCHWARZ K,et al.Response and recovery of fathead minnows(Pimephales promelas)following early life exposure to water and sediment found within agricultural runoff from the Elkhorn River,Nebraska,USA[J].Sci Total Environ,2018,618:1371-1381.
[40]ADAMEK M,HAZERLI D,MATRAS M,et al.Viral infections in common carp lead to a disturbance of mucin expression in mucosal tissues[J].Fish Shellfish Immunol,2017,71:353-358.
[41]黄建盛,陈刚,张健东,等.基于响应面法分析温度和盐度对虎斑乌贼受精卵孵化的联合影响[J].生态学杂志,2016,35(2):446-452.
[42]赵强,余四九,王廷璞,等.响应面法优化秃疮花中生物碱提取工艺及抑菌活性研究[J].草业学报,2012,21(4):206-214.
[43]钱佳慧,栗志民,申玉春,等.温度和盐度对华贵栉孔扇贝抗氧化酶活性的联合效应研究[J].南方水产科学,2015,11(6):49-57.
[44]黄建盛,陈刚,张健东,等.响应曲面法分析低盐条件下温度与pH对斜带石斑鱼幼鱼能量代谢的联合效应[J].海洋渔业,2017,39(1):41-50.