多环芳烃降解菌的筛选、鉴定及其特性
|
摘要
从黄河三角洲东营胜利油田附近石油污染的土壤中分离、筛选到菲降解菌共6株,并对其中降解率相对较高的菌株PAHs-1进行了生物学特性的研究,包括菌体形态特征、培养特征及生理生化特征的初步研究.通过形态观察、生理生化指标及16S rDNA序列分析,鉴定该菌株为短芽孢杆菌属.研究所筛选的PAHs-1能够以多环芳烃菲为唯一碳源和能源,7 d内对菲(培养液浓度为50 mg/L)的降解率达到62.09%.并通过试验确定了PAHs-1对菲的最佳降解pH为7.另外,研究中一系列试验表明随着外源碳源葡萄糖的加入,PAHs-1对菲的降解率呈微小的增大趋势,而随着菲浓度的增加,PAHs-1对菲的降解率呈减小趋势.
In this study, six strains of phenanthrene degrading bacteria were isolated and screened from oil-polluted soid near dongying Shengli oilfield in the Yellow River delta. The biological characteristics for relatively high degradation rate of PAHs-1 strain were studied, including preliminary morphological studies, the physiological and biochemical characteristics of culture and bacteria. The strain was identified as short bacillus by morphological observation, physiological and biochemical indexes and 16 S rDNA sequence analysis. PAHs-1 screened in this study can be used as the only carbon source and energy source to degrade phenanthrene(medium concentration of 50 mg/L) at a rate of 62.09% within 7 days. The optimum pH for PAHs-1 degradation of phenanthrene was 7. In addition, a series of experiments in this study showed that, with the addition of exogenous carbon source glucose, the degradation rate of phenanthrene showed a slight upward trend, while with the increase of phenanthrene concentration, the degradation rate of phenanthrene showed a downward trend.
In this study, six strains of phenanthrene degrading bacteria were isolated and screened from oil-polluted soid near dongying Shengli oilfield in the Yellow River delta. The biological characteristics for relatively high degradation rate of PAHs-1 strain were studied, including preliminary morphological studies, the physiological and biochemical characteristics of culture and bacteria. The strain was identified as short bacillus by morphological observation, physiological and biochemical indexes and 16 S rDNA sequence analysis. PAHs-1 screened in this study can be used as the only carbon source and energy source to degrade phenanthrene(medium concentration of 50 mg/L) at a rate of 62.09% within 7 days. The optimum pH for PAHs-1 degradation of phenanthrene was 7. In addition, a series of experiments in this study showed that, with the addition of exogenous carbon source glucose, the degradation rate of phenanthrene showed a slight upward trend, while with the increase of phenanthrene concentration, the degradation rate of phenanthrene showed a downward trend.
引文
[1] 郭志明,刘頔,林田,等.太原城区PM2.5中多环芳烃、硝基多环芳烃的污染特征、来源解析和健康风险评价[J].环境科学学报,2018,38(3):1103-1108.
[2] Wang B C,Wang Q H,Zhu L C,et al.Degrade naphthalene using cells immobilized combining with low-intensity ultrasonic technique[J].Colloid & Surfaces B Biointerfaces,2007,57(1):17-21.
[3] Mcveety B D,Hites R A.The distribution and accumulation of PAHs in environment[J].Atoms Environ,1988,22(1):511-536.
[4] 惠云芳,王鸿飞.石油污染土壤的生物修复研究进展[J].西北农业学报,2018,27(4):451-458.
[5] 刘春晓,阎光绪,郭绍辉,等.微生物降解土壤中石油污染物的研究进展[J].污染防治技术,2007,20(6):51-54,97.
[6] 杨革.微生物学实验教程[M].第2版.北京:科学出版社,2009.
[7] 曹雪娇,房宇,邹永春,等.镉与萘单一和复合作用对穗花狐尾藻生长的影响[J].曲阜师范大学学报(自然科学版),2017,43(2):70-73.
[8] Shuchi V,Renu B,Vikas P.Oily sludge degradation by bacteria from ankleshwar india[J].International Biodeterioration & Biodegradation,2006,57:207-213.
[9] Gilichinsky D A,Wagener.Microbial life in permafrost:A historical Review[J].Permafrost & Periglacial Processes,1995,6(3):243 -250.
[10] Gibson D T,Mahadevan V,Jerina D M,et al.Oxidation of the carcinogens benzo [a] pyrene and benzo [a] anthracene to dihydrodiols by a bacterium[J].Science,1975,189(4199):295-297.
[11] 杨辉,王海霞,李晓军,等.多环芳烃污染土壤生物修复技术研究进展[J].安徽农业科学,2011,39(3):1427-1429,1431.
[12] 陶雪琴,卢桂宁,易筱筠,等.菲高效降解菌的筛选及其降解中间产物分析[J].农业环境科学学报,2006,25(1):190-195.
[2] Wang B C,Wang Q H,Zhu L C,et al.Degrade naphthalene using cells immobilized combining with low-intensity ultrasonic technique[J].Colloid & Surfaces B Biointerfaces,2007,57(1):17-21.
[3] Mcveety B D,Hites R A.The distribution and accumulation of PAHs in environment[J].Atoms Environ,1988,22(1):511-536.
[4] 惠云芳,王鸿飞.石油污染土壤的生物修复研究进展[J].西北农业学报,2018,27(4):451-458.
[5] 刘春晓,阎光绪,郭绍辉,等.微生物降解土壤中石油污染物的研究进展[J].污染防治技术,2007,20(6):51-54,97.
[6] 杨革.微生物学实验教程[M].第2版.北京:科学出版社,2009.
[7] 曹雪娇,房宇,邹永春,等.镉与萘单一和复合作用对穗花狐尾藻生长的影响[J].曲阜师范大学学报(自然科学版),2017,43(2):70-73.
[8] Shuchi V,Renu B,Vikas P.Oily sludge degradation by bacteria from ankleshwar india[J].International Biodeterioration & Biodegradation,2006,57:207-213.
[9] Gilichinsky D A,Wagener.Microbial life in permafrost:A historical Review[J].Permafrost & Periglacial Processes,1995,6(3):243 -250.
[10] Gibson D T,Mahadevan V,Jerina D M,et al.Oxidation of the carcinogens benzo [a] pyrene and benzo [a] anthracene to dihydrodiols by a bacterium[J].Science,1975,189(4199):295-297.
[11] 杨辉,王海霞,李晓军,等.多环芳烃污染土壤生物修复技术研究进展[J].安徽农业科学,2011,39(3):1427-1429,1431.
[12] 陶雪琴,卢桂宁,易筱筠,等.菲高效降解菌的筛选及其降解中间产物分析[J].农业环境科学学报,2006,25(1):190-195.