不同病理类型及分期甲状腺癌中miR-221 miR-222 miR-197和miR-346的表达研究
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摘要
目的探讨不同病理类型及分期甲状腺癌中miR-221、miR-222、miR-197和miR-346的表达水平,为临床手术方式选择及术后治疗提供参考。方法选取2013年6月～2017年8月行甲状腺手术的40例甲状腺癌(乳头状癌、滤泡癌各20例)及癌旁正常甲状腺组织、20例良性甲状腺疾病组织,术中取材后,立即放入液氮中保存后转入-70℃保存。采用定量RT-PCR检测miR-221、miR-222、miR-197和miR-346在相应组织标本中的表达水平。结果对甲状腺癌,miR-221与miR-222的表达与患者的年龄、性别、最大肿瘤灶等无明显相关性(P>0.05),与TNM分期及淋巴转移情况相关(P<0.05);miR-197和miR-346的表达在20例甲状腺滤泡癌中均有明显升高(P<0.05),乳头状癌组织中未见显著变化(P>0.05)。miR-221与miR-222在良性甲状腺疾病组织表达量变化均不明显(P>0.05);但在甲状腺癌组织中的表达变化显著,差异均有统计学意义(P<0.05);对miR-197和miR-346在相应组织标本中的表达水平进行比较结果显示,良性甲状腺疾病组织中miR-197和miR-346表达没有明显变化(P>0.05);在20例滤泡癌组织中明显升高,差异有统计学意义(P<0.05);在乳头状癌组织及癌旁正常组织中的均无明显变化(P>0.05)。结论不同病理类型及分期甲状腺癌中miRNA的表达可作为甲状腺癌早期诊断、淋巴结转移、复发及远处转移密切相关的预警分子,为甲状腺癌手术方式选择及术后指导治疗提供了重要依据。
Objective To analyze and discuss the expression of miRNA in different pathological types and stages of thyroid carcinoma,and provide reference for the clinical operation and postoperative treatment.Methods 40 patients of thyroid carcinoma(including 20 cases of papillary carcinoma)were collected from people's Hospital of Inner Mongolia Autonomous region and affiliated Hospital of Inner Mongolia Medical University.20 cases of follicular carcinoma)and 20 cases of benign thyroid disease tissues were collected during operation.After being stored in liquid nitrogen immediately,it was transferred to-70℃for storage.The quantitative RT-PCR was used to detect miR-221 and miR-222.The expression level of miR-197 and miR-346 in corresponding tissues.Results For thyroid cancer,miR-221 and miR-222 expression and patient age and gender,had no significant correlation between the maximum tumor foci.They were correlated with TNM staging(P<0.05)and lymph node metastasis(P<0.05).miR-197 and miR-346 expression significantly increased in thyroid follicular carcinoma(P<0.05).The expression of miR-221 and miR-222 in benign thyroid diseases did not change significantly(P>0.05).However,there was a significant change in the expression of PTNE in thyroid carcinoma,There was a negative correlation between them and the expression of PTNE(P<0.05).The difference was statistically significant(P< 0.05).The expression levels of miR-197 and miR-346 in corresponding tissues were compared.There was no significant change in the expression of miR-197 and miR-346 in benign thyroid diseases.In thyroid carcinomas 2 There was a significant increase in 0 cases of follicular carcinoma,the difference was statistically significant(P<0.05),and there was no significant change in the tissues of papillary carcinoma and normal tissues adjacent to the carcinoma.Conclusion The expression of miRNA in different pathological types and stages of thyroid carcinoma is closely related to early diagnosis,lymph node metastasis,recurrence and distant metastasis of thyroid carcinoma.
Objective To analyze and discuss the expression of miRNA in different pathological types and stages of thyroid carcinoma,and provide reference for the clinical operation and postoperative treatment.Methods 40 patients of thyroid carcinoma(including 20 cases of papillary carcinoma)were collected from people's Hospital of Inner Mongolia Autonomous region and affiliated Hospital of Inner Mongolia Medical University.20 cases of follicular carcinoma)and 20 cases of benign thyroid disease tissues were collected during operation.After being stored in liquid nitrogen immediately,it was transferred to-70℃for storage.The quantitative RT-PCR was used to detect miR-221 and miR-222.The expression level of miR-197 and miR-346 in corresponding tissues.Results For thyroid cancer,miR-221 and miR-222 expression and patient age and gender,had no significant correlation between the maximum tumor foci.They were correlated with TNM staging(P<0.05)and lymph node metastasis(P<0.05).miR-197 and miR-346 expression significantly increased in thyroid follicular carcinoma(P<0.05).The expression of miR-221 and miR-222 in benign thyroid diseases did not change significantly(P>0.05).However,there was a significant change in the expression of PTNE in thyroid carcinoma,There was a negative correlation between them and the expression of PTNE(P<0.05).The difference was statistically significant(P< 0.05).The expression levels of miR-197 and miR-346 in corresponding tissues were compared.There was no significant change in the expression of miR-197 and miR-346 in benign thyroid diseases.In thyroid carcinomas 2 There was a significant increase in 0 cases of follicular carcinoma,the difference was statistically significant(P<0.05),and there was no significant change in the tissues of papillary carcinoma and normal tissues adjacent to the carcinoma.Conclusion The expression of miRNA in different pathological types and stages of thyroid carcinoma is closely related to early diagnosis,lymph node metastasis,recurrence and distant metastasis of thyroid carcinoma.
引文
[1]王志宏.MicroRNA对甲状腺乳头状癌侵袭性的影响及其机制研究[D].中国医科大学,2014:1-67.
[2]孙辉,刘晓莉.甲状腺癌规范化诊治理念更新及其意义[J].中国实用外科杂志,2015,35(1):72-75.
[3] Michael H,Hsieh,Ramiro J,et al.Cross-sectional evaluation of parental decision making factors for vesicoureteral reflux management in children[J].The Journal of urology,2010,184(4Suppl):1589-1593.
[4]李寅辉.MicroRNA与甲状腺癌的关系[J].医学综述,2014,20(10):1732-1734.
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[6]徐莹.甲状腺癌患者特异血清miRNAs的筛选及其临床意义研究[D].南京大学,2012:1-50.
[7] Jill,Koshiol,Martha,et al.Evaluation of a multiplex panel of immune-related markers in cervical secretions:a methodologic study[J].International journal of cancer.Journal international du cancer,2014,134(2):411-425.
[8]肖海鹏,喻爽.甲状腺肿瘤与循环RNA研究进展[J].中国癌症杂志,2016,(1):25-30.
[9]张顺霞,李淑霞,王江,等.人桥本甲状腺炎甲状腺组织中miRNAs表达谱的差异性分析[J].现代生物医学进展,2015,15(36):7171-7174.
[10] YK Chen,CL Lin,FT Cheng,et al.Cancer risk in patients with Hashimoto's thyroiditis:A nationwide cohort study[J].2013,9(9):2496-2501.
[11]张德言.甲状腺乳头状癌差异表达microRNAs的筛选及其功能初步研究[D].郑州大学,2011:1-64.
[12]丛小飞.桥本甲状腺炎合并乳头状甲状腺癌中miRNA表达差异的研究[D].青岛大学,2014:1-43.
[13]王苏贵.前列腺癌去势抵抗转化相关miRNA及其调控网络的整合分析[D].苏州大学,2013:1-77.
[14]黄金球.MiRNAs在大鼠肾移植急性排斥反应中的早期诊断作用[D].南方医科大学,2013:1-71.
[15]李寅辉.MicroRNA与甲状腺癌的关系[J].医学综述,2014,20(10):1732-1734.
[16]覃宇周.结直肠癌hTERT调控相关miRNA的筛选与初步鉴定[D].广西医科大学,2016:1-128.
[17]谢学成,邱海,汤帆,等.端粒酶逆转录酶在结直肠癌组织中的表达及其临床意义[J].中国癌症防治杂志,2014,(3):252-257.
[18] Izgi A,Gunal A,Yalcin S,et al.Telomere 1(POT1)gene expression and its association with telomerase activity in colorectal tumor samples with different pathological features[J].Biomedicine&Pharmacotherapy,2014,68(7):841-846.
[19]刘亮,陈建,刘凤军.结肠癌中hTERT的表达及其与Survivin和bcl-2表达的关系[J].中国现代普通外科进展,2010,13(3):173-176.
[20] Weber F,Teresi RE,Broelsch CE,et al.A limited set of human MicroRNA is deregulated in follicular thyroid carcinoma[J].J Clin Endocrinol Metab,2006,91(9):3584-3591.
[2]孙辉,刘晓莉.甲状腺癌规范化诊治理念更新及其意义[J].中国实用外科杂志,2015,35(1):72-75.
[3] Michael H,Hsieh,Ramiro J,et al.Cross-sectional evaluation of parental decision making factors for vesicoureteral reflux management in children[J].The Journal of urology,2010,184(4Suppl):1589-1593.
[4]李寅辉.MicroRNA与甲状腺癌的关系[J].医学综述,2014,20(10):1732-1734.
[5]秦青青,李婷婷,郑永钦,等.microRNA在甲状腺乳头状癌中作用机制的研究进展[J].基础医学与临床,2017,37(8):1184-1189.
[6]徐莹.甲状腺癌患者特异血清miRNAs的筛选及其临床意义研究[D].南京大学,2012:1-50.
[7] Jill,Koshiol,Martha,et al.Evaluation of a multiplex panel of immune-related markers in cervical secretions:a methodologic study[J].International journal of cancer.Journal international du cancer,2014,134(2):411-425.
[8]肖海鹏,喻爽.甲状腺肿瘤与循环RNA研究进展[J].中国癌症杂志,2016,(1):25-30.
[9]张顺霞,李淑霞,王江,等.人桥本甲状腺炎甲状腺组织中miRNAs表达谱的差异性分析[J].现代生物医学进展,2015,15(36):7171-7174.
[10] YK Chen,CL Lin,FT Cheng,et al.Cancer risk in patients with Hashimoto's thyroiditis:A nationwide cohort study[J].2013,9(9):2496-2501.
[11]张德言.甲状腺乳头状癌差异表达microRNAs的筛选及其功能初步研究[D].郑州大学,2011:1-64.
[12]丛小飞.桥本甲状腺炎合并乳头状甲状腺癌中miRNA表达差异的研究[D].青岛大学,2014:1-43.
[13]王苏贵.前列腺癌去势抵抗转化相关miRNA及其调控网络的整合分析[D].苏州大学,2013:1-77.
[14]黄金球.MiRNAs在大鼠肾移植急性排斥反应中的早期诊断作用[D].南方医科大学,2013:1-71.
[15]李寅辉.MicroRNA与甲状腺癌的关系[J].医学综述,2014,20(10):1732-1734.
[16]覃宇周.结直肠癌hTERT调控相关miRNA的筛选与初步鉴定[D].广西医科大学,2016:1-128.
[17]谢学成,邱海,汤帆,等.端粒酶逆转录酶在结直肠癌组织中的表达及其临床意义[J].中国癌症防治杂志,2014,(3):252-257.
[18] Izgi A,Gunal A,Yalcin S,et al.Telomere 1(POT1)gene expression and its association with telomerase activity in colorectal tumor samples with different pathological features[J].Biomedicine&Pharmacotherapy,2014,68(7):841-846.
[19]刘亮,陈建,刘凤军.结肠癌中hTERT的表达及其与Survivin和bcl-2表达的关系[J].中国现代普通外科进展,2010,13(3):173-176.
[20] Weber F,Teresi RE,Broelsch CE,et al.A limited set of human MicroRNA is deregulated in follicular thyroid carcinoma[J].J Clin Endocrinol Metab,2006,91(9):3584-3591.