两种狂犬病毒IgG抗体检测试剂盒的比较
|
摘要
目的了解员工暴露前接种人用狂犬病疫苗后的免疫效果,并对比使用两种不同试剂盒检测抗体阳转率是否存在差异。方法采集员工接种人用狂犬病疫苗(地鼠肾细胞)以及冻干人用狂犬病疫苗(Vero细胞)共计172例,分别使用两种狂犬病毒抗体检测试剂盒(试剂盒A、B)进行检测,统计血清中狂犬病毒Ig G抗体的水平,计算阳转率并比较差异。结果使用试剂盒A检测接种人用狂犬病疫苗(地鼠肾细胞)者血清样本的阳转率为91.7%,接种冻干人用狂犬病疫苗(Vero细胞)者血清样本的阳转率为51.0%;使用试剂盒B检测接种人用狂犬病疫苗(地鼠肾细胞)者血清样本的阳转率为100.0%,接种冻干人用狂犬病疫苗(Vero细胞)者血清样本的阳转率为74.5%。使用试剂盒B检测接种人用狂犬病疫苗(地鼠肾细胞)者血清样本的阳转率比试剂盒A高8.3%,使用试剂盒B检测接种冻干人用狂犬病疫苗(Vero细胞)者血清样本的阳转率比试剂盒A高23.5%。结论两种不同试剂盒上检测的抗体阳转率都反映出人用狂犬病疫苗(地鼠肾细胞)比冻干人用狂犬病疫苗(Vero细胞)的免疫效果好;使用两种不同试剂盒检测抗体阳转率的差异均具有统计学意义。
Objective To understand the effect of immunization with rabies vaccines for human use before exposure and to compare the difference in seroconversion rate for antibody against rabies virus between 2 different detection kits. Methods A total of 172 staff members were inoculated PHK cell( hamster-kidney-cell) rabies vaccines and Vero-cell rabies vaccines,respectively,which were detected Ig G antibody level in serum by 2 kinds of antibody detection kits( A and B) against rabies virus,and seroconversion rate were evaluated and the difference compared. Results The detected seroconversion rate was 91.7% for staff members inoculated PHK cell rabies vaccine,the same rate was 51.0% for staff members inoculated Vero-cell rabies vaccine by both using kit A. The detected seroconversion rate was 100% for staff members inoculated PHK cell rabies vaccine,the same rate was 74.5% for staff members inoculated Vero-cell rabies vaccine by both using kit B.The seroconversion rate was 8.3% higher by using kit B than kit A for staff members inoculated PHK cell rabies vaccine,the seroconversion rate was 23.5% higher by using kit B than kit A for staff members inoculated Vero-cell rabies vaccine. Conclusion The detected seroconversion rates showed that PHK rabies vaccine was better than the Vero-cell rabies vaccine.There were significant differences in detection of antibody seroconversion rate by both of detection kits.
Objective To understand the effect of immunization with rabies vaccines for human use before exposure and to compare the difference in seroconversion rate for antibody against rabies virus between 2 different detection kits. Methods A total of 172 staff members were inoculated PHK cell( hamster-kidney-cell) rabies vaccines and Vero-cell rabies vaccines,respectively,which were detected Ig G antibody level in serum by 2 kinds of antibody detection kits( A and B) against rabies virus,and seroconversion rate were evaluated and the difference compared. Results The detected seroconversion rate was 91.7% for staff members inoculated PHK cell rabies vaccine,the same rate was 51.0% for staff members inoculated Vero-cell rabies vaccine by both using kit A. The detected seroconversion rate was 100% for staff members inoculated PHK cell rabies vaccine,the same rate was 74.5% for staff members inoculated Vero-cell rabies vaccine by both using kit B.The seroconversion rate was 8.3% higher by using kit B than kit A for staff members inoculated PHK cell rabies vaccine,the seroconversion rate was 23.5% higher by using kit B than kit A for staff members inoculated Vero-cell rabies vaccine. Conclusion The detected seroconversion rates showed that PHK rabies vaccine was better than the Vero-cell rabies vaccine.There were significant differences in detection of antibody seroconversion rate by both of detection kits.
引文
[1]俞永新,朱家鸿,徐葛林,等.狂犬病和狂犬病疫苗[M].2版.北京:中国医药科技出版社,2009.
[2]中国疾病预防控制中心.狂犬病预防控制技术指南(2016版)[J].中国病毒病杂志,2016,6(03):161-188.
[3]黄学勇,李幸乐,张白帆,等.人抗狂犬病病毒Ig G抗体3种检测系统的可比性研究[J].中国卫生检验杂志,2015,25(8):1172-1174.
[4]王伟伟,王远征,张国强,等.狂犬病病毒抗体竞争ELISA检测方法的建立及初步应用[J].中国生物制品学杂志,2011,24(11):1355-1359.
[5]董关木,郑海发,刘增顺,等.我国纯化狂犬病疫苗的临床反应及效果评价[J].中国生物制品学杂志,2002,15(5):312-314.
[6]周志军,尹斌,朱华松,等.ELISA抗体检测试剂盒在狂犬病毒特免血浆筛选中的初步应用[J].微生物学免疫学进展,2005,33(2):45-48.
[7]苏军英,陶小润,孙承梅,等.人用狂犬病疫苗免疫抗体检测方法的比较[J].中国生物制品学杂志,1999,12(4):236-237.
[2]中国疾病预防控制中心.狂犬病预防控制技术指南(2016版)[J].中国病毒病杂志,2016,6(03):161-188.
[3]黄学勇,李幸乐,张白帆,等.人抗狂犬病病毒Ig G抗体3种检测系统的可比性研究[J].中国卫生检验杂志,2015,25(8):1172-1174.
[4]王伟伟,王远征,张国强,等.狂犬病病毒抗体竞争ELISA检测方法的建立及初步应用[J].中国生物制品学杂志,2011,24(11):1355-1359.
[5]董关木,郑海发,刘增顺,等.我国纯化狂犬病疫苗的临床反应及效果评价[J].中国生物制品学杂志,2002,15(5):312-314.
[6]周志军,尹斌,朱华松,等.ELISA抗体检测试剂盒在狂犬病毒特免血浆筛选中的初步应用[J].微生物学免疫学进展,2005,33(2):45-48.
[7]苏军英,陶小润,孙承梅,等.人用狂犬病疫苗免疫抗体检测方法的比较[J].中国生物制品学杂志,1999,12(4):236-237.