套袋对不知火果实品质和果皮类胡萝卜素代谢的影响
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摘要
为研究套袋对不知火果实品质和类胡萝卜素代谢的影响,选用生产上常用的外黄内黑双层纸袋对不知火果实进行套袋处理,以不套袋为对照.果实品质相关指标测定结果表明:不知火果实套袋后果实单质量增加,可滴定酸和可溶性固形物质量分数降低,Vc质量浓度、出汁率和可食率变化不大.利用HPLC检测对套袋和未套袋果实果皮紫黄质、9-z-紫黄质、β-隐黄质、a-胡萝卜素、β-胡萝卜素和八氢番茄红素质量比进行了测定,发现套袋果实果皮中这些类胡萝卜素类物质质量比均低于未套袋果实.利用实时荧光定量PCR研究了套袋对果皮CRTISO,LCYb,LCYe,PDS,PSY,ZDS和ZEP基因表达的影响,发现:套袋不知火果皮中这些基因的表达均显著低于未套袋果实.该研究结果表明:套袋可提高不知火外观品质,对不知火果实质量的增加具有一定的促进作用,同时,套袋使不知火果实风味下降.此外,该研究发现套袋还显著降低了不知火果皮类胡萝卜素质量比,这可能是由于果实套袋使得阳光无法透过,进而使得类胡萝卜素代谢相关基因的表达降低引起的.
The effects of bagging on fruit quality of Shiranui-a hybrid of Citrus reticulata-and its peel carotenoid metabolism were investigated.The results of fruit quality-related indexes showed that bagging increased fruit weight and decreased titratable acid and soluble solid contents,while fruit Vc content,juice yield and edible rate were little influenced.HPLC showed that all the carotenoid-related pigments(violaxanthin,9-z-violaxathin,β-cryptoxanthin,a-carotene,β-carotene and phytoene)were significantly lower in bagged fruit peel than in non-bagged fruit peel.QRT-PCR showed that the expression levels of the carotenogenesis genes(CRTISO,LCYb,LCYe,PDS,PSY,PDSand ZEP)were all significantly down-regulated in the peel of the bagged Shiranui fruit compared with the non-bagged fruit.The above results indicated that bagging of fruit could be helpful for improving fruit appearance quality,but it would deteriorate fruit flavor.In addition,bagging suppressed carotenoid metabolism in fruit peel,which may have resulted from the inhibition effect of bagging on light transmission and carotenogenesis gene expression.
The effects of bagging on fruit quality of Shiranui-a hybrid of Citrus reticulata-and its peel carotenoid metabolism were investigated.The results of fruit quality-related indexes showed that bagging increased fruit weight and decreased titratable acid and soluble solid contents,while fruit Vc content,juice yield and edible rate were little influenced.HPLC showed that all the carotenoid-related pigments(violaxanthin,9-z-violaxathin,β-cryptoxanthin,a-carotene,β-carotene and phytoene)were significantly lower in bagged fruit peel than in non-bagged fruit peel.QRT-PCR showed that the expression levels of the carotenogenesis genes(CRTISO,LCYb,LCYe,PDS,PSY,PDSand ZEP)were all significantly down-regulated in the peel of the bagged Shiranui fruit compared with the non-bagged fruit.The above results indicated that bagging of fruit could be helpful for improving fruit appearance quality,but it would deteriorate fruit flavor.In addition,bagging suppressed carotenoid metabolism in fruit peel,which may have resulted from the inhibition effect of bagging on light transmission and carotenogenesis gene expression.
引文
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[14]张秋明,丁伟平,郑玉生,等.套袋对脐橙果实品质的影响[J].湖南农业大学学报(自然科学版),2002,28(5):402-404.
[15]王武,邓烈,何绍兰,等.不同套袋处理对北碚447锦橙果实色泽的影响[J].西南大学学报(自然科学版),2009,31(2):65-69.
[16]朱春华,李进学,周东果,等.套袋对反季柠檬品质变化的影响[J].西南农业学报,2011,24(1):202-205.
[17]HUANG C,YU B,TENG Y,et al.Effects of Fruit Bagging on Coloring and Related Physiology,and Qualities of Red Chinese Sand Pears During Fruit Maturation[J].Scientia Horticulturae,2009,121(2):149-158.
[18]王少敏,高华君,刘嘉芬,等.套袋短枝红富士果实内含物及果皮色素的变化[J].果树科学,2000,17(1):76-77.
[19]淳长品,彭良志,曹立,等.不同纸袋套袋对纽荷尔脐橙果实品质的效应研究[J].西南大学学报(自然科学版),2008,30(4):106-110.
[20]王涛,林媚,陈丹霞,等.套袋对温州蜜柑果实营养成分的影响[J].安徽农学通报,2007,13(9):68-69.
[21]张义刚,张龙天,杨丽,等.不同套袋处理对尤力克柠檬果实品质的影响[J].西南农业学报,2012,25(5):1947-1949.
[22]陶俊,张上隆,安新民,等.光照对柑橘果皮类胡萝卜素和色泽形成的影响[J].应用生态学报,2003,14(11):1833-1836.
[23]GIULIANO G,BARTLEY G E,SCOLNIK P A.Regulation of Carotenoid Biosynthesis During Tomato Development[J].The Plant Cell,1993,5(4):379-387.
[24]刘宝贞.培养条件对柑橘胚性愈伤组织中类胡萝卜素合成的影响[D].武汉:华中农业大学,2009.
[2]陈天礼.不知火橘橙的越冬栽培技术[J].浙江柑橘,2007,24(1):23-25.
[3]张秋明,丁伟平,郑玉生,等.套袋对脐橙果实品质的影响[J].湖南农业大学学报(自然科学版),2002,28(5):402-404.
[4]黄勇.不知火橘橙果实黄色突变分子机理研究[D].重庆:西南大学,2011.
[5]徐娟.几个柑桔产区果实色泽评价及红肉脐橙(Citrus sinensis L.cv.Cara cara)果肉呈色机理初探[D].武汉:华中农业大学,2002.
[6]CHENG C Z,YANG J W,YAN H B,et al.Expressing p20 Hairpin RNA of Citrus tristeza virus Confers Citrus aurantium with Tolerance/Resistance Against Stem Pitting and Seedling Yellow CTV Strains[J].Journal of Integrative Agriculture,2015,14(9):1767-1777.
[7]YANG W H,ZHU X C,BU J H,et al.Effects of Bagging on Fruit Development and Quality in Cross-Winter Off-Season Longan[J].Scientia Horticulturae,2009,120(2):194-200.
[8]郑小林,张佳佳,励建荣.‘凯特’杧果栽培中果实套袋对其采后品质及贮藏性的影响[J].园艺学报,2011,38(4):657-665.
[9]武红霞,王松标,石胜友,等.不同套袋材料对红杧6号杧果果实品质的影响[J].果树学报,2009,26(5):644-648.
[10]SHARMA R R,PAL R K,ASREY R,et al.Pre-Harvest Fruit Bagging Influences Fruit Color and Quality of Apple cv.Delicious[J].Agricultural Sciences,2013,4(9):443-448.
[11]JIA H J,ARAKI A,OKAMOTO G.Influence of Fruit Bagging on Aroma Volatiles and Skin Coloration of‘Hakuho’Peach(Prunus persica Batsch)[J].Postharvest Biology and Technology,2005,35(1):61-68.
[12]李平,温华良,郑润泉,等.套袋对番石榴果实品质的影响[J].亚热带植物科学,2003,32(1):17-19.
[13]冯健君,陈俊伟,徐红霞,等.果袋透光性对宁海白枇杷果实品质及抗氧化能力的影响[J].果树学报,2009,26(1):66-70.
[14]张秋明,丁伟平,郑玉生,等.套袋对脐橙果实品质的影响[J].湖南农业大学学报(自然科学版),2002,28(5):402-404.
[15]王武,邓烈,何绍兰,等.不同套袋处理对北碚447锦橙果实色泽的影响[J].西南大学学报(自然科学版),2009,31(2):65-69.
[16]朱春华,李进学,周东果,等.套袋对反季柠檬品质变化的影响[J].西南农业学报,2011,24(1):202-205.
[17]HUANG C,YU B,TENG Y,et al.Effects of Fruit Bagging on Coloring and Related Physiology,and Qualities of Red Chinese Sand Pears During Fruit Maturation[J].Scientia Horticulturae,2009,121(2):149-158.
[18]王少敏,高华君,刘嘉芬,等.套袋短枝红富士果实内含物及果皮色素的变化[J].果树科学,2000,17(1):76-77.
[19]淳长品,彭良志,曹立,等.不同纸袋套袋对纽荷尔脐橙果实品质的效应研究[J].西南大学学报(自然科学版),2008,30(4):106-110.
[20]王涛,林媚,陈丹霞,等.套袋对温州蜜柑果实营养成分的影响[J].安徽农学通报,2007,13(9):68-69.
[21]张义刚,张龙天,杨丽,等.不同套袋处理对尤力克柠檬果实品质的影响[J].西南农业学报,2012,25(5):1947-1949.
[22]陶俊,张上隆,安新民,等.光照对柑橘果皮类胡萝卜素和色泽形成的影响[J].应用生态学报,2003,14(11):1833-1836.
[23]GIULIANO G,BARTLEY G E,SCOLNIK P A.Regulation of Carotenoid Biosynthesis During Tomato Development[J].The Plant Cell,1993,5(4):379-387.
[24]刘宝贞.培养条件对柑橘胚性愈伤组织中类胡萝卜素合成的影响[D].武汉:华中农业大学,2009.