高效毛细管电泳测定全蝎中牛磺酸的含量
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摘要
目的:对全蝎中的牛磺酸进行含量测定,以建立活性导向的指纹图谱。方法:采用高效毛细管电泳(HPCE)法,使用熔融硅胶毛细管柱(75μm i.d.,总长50cm,有效长度40cm),以10mmoL硼酸钠(pH9.0)为缓冲液,以牛磺酸为指标,对全蝎20%乙醇超声提取液进行含量测定并分析各批次药材中该成分的含量。结果:方法线性、精密度、准确度和样品稳定性良好;6批全蝎药材中,牛磺酸的平均含量为7.783mg/g。结论:该方法快速、准确、高效,可作为全蝎的质量控制方法。
Objective: To determine the taurine content in Buthus martensii Karsch and establish activity-oriented fingerprints. Methods: Determination was carried out with high-performance capillary electrophoresis(HPCE), using fused-silica capillary(75μm i.d., total length: 50 cm, effective length: 40 cm) with 10 mmoL of sodium borate(pH 9.0) as buffer solution, taurine was used as the index to determine the content of 20% ethanol ultrasonic extract of Buthus martensii Karsch and analyze the contents of this component in each batch of medicinal materials. Results: The analytical method had good linearity, precision, accuracy and sample stability; among 6 batches of Buthus martensii Karsch samples, the average content of taurine was 7.783 mg/g. Conclusion: This method is fast, accurate and effective, which is suitable for quality control of Buthus martensii Karsch.
Objective: To determine the taurine content in Buthus martensii Karsch and establish activity-oriented fingerprints. Methods: Determination was carried out with high-performance capillary electrophoresis(HPCE), using fused-silica capillary(75μm i.d., total length: 50 cm, effective length: 40 cm) with 10 mmoL of sodium borate(pH 9.0) as buffer solution, taurine was used as the index to determine the content of 20% ethanol ultrasonic extract of Buthus martensii Karsch and analyze the contents of this component in each batch of medicinal materials. Results: The analytical method had good linearity, precision, accuracy and sample stability; among 6 batches of Buthus martensii Karsch samples, the average content of taurine was 7.783 mg/g. Conclusion: This method is fast, accurate and effective, which is suitable for quality control of Buthus martensii Karsch.
引文
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[8]张声俊,许长平,陈建斌,等.全蝎不同部位的红外光谱分析与评价[J].光谱学与光谱分析,2011,31(10):2711-2714.
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[2]赵海梅,左志琴,程绍民,等.全蝎、蜈蚣对胶原诱导型关节炎大鼠小肠黏膜IL-2、IL-4、IL-10表达及关节损伤的影响[J].中国中西医结合杂志,2012,32(1):80-83.
[3]彭延古,许光明,徐爱良,等.全蝎抗凝活性成分的研究[J].湖南中医学院学报,2006,26(1):16-17,20.
[4]彭延古,雷田香,李路丹,等.全蝎提取液对家兔实验性动脉血栓的影响[J].微循环学杂志,2006,16(4):59-60.
[5]郑晓媚,黄瑶,王新雨,等.HPLC-ELSD法测定全蝎中牛磺酸的含量[J].中药新药与临床药理,2016,27(3):422-424.
[6]王聪聪,李峰,史磊,等.全蝎加工提取和含量测定方法研究进展[J].山东中医杂志,2015,34(1):77-79.
[7]崔聪,周洪雷,王洪平,等.HPLC法测定全蝎中胆甾醇含量[J].辽宁中医药大学学报,2011,13(12):46-49.
[8]张声俊,许长平,陈建斌,等.全蝎不同部位的红外光谱分析与评价[J].光谱学与光谱分析,2011,31(10):2711-2714.
[9]Kapnissi-Christodoulou CP,Zhu X,Warner IM.Analytical separations in open-tubular capillary electrochromatography[J].Electrophoresis,2003,24(22-23):3917-3934.
[10]Eeltink S,Kok WT.Recent applications in capillary electrochromatography[J].Electrophoresis,2006,27(1):84-96.