京墨中有害元素的含量测定及挥发油成分分析
|
摘要
目的:测定京墨药材中砷、铅、镉、铬、锑、铜、汞7种有害元素的含量,对京墨药材挥发油成分进行定性分析。方法:京墨样品经微波消解后,以电感耦合等离子体-原子发射光谱法(ICP-AES)测定京墨中砷、铅、镉、铬、锑和铜元素含量,以原子荧光光度法测定汞元素含量;用水蒸气蒸馏法制备京墨挥发油样品,GC-MS定性分析京墨药材挥发油成分。结果:微波消解后ICP-AES测定方法线性关系、精密度和重现性良好,回收率为86. 70%～104. 37%,RSD均≤5. 0%;挥发油中鉴定出7种稠环芳烃类化合物。结论:根据中国《药用植物及制剂进出口绿色行业标准》规定,京墨药材中重金属存在超标现象;京墨挥发油成分中主要为多环芳烃类化合物,大多具有致癌性及致畸性。
Objective To establish analysis methods for determination of 7 heavy metals(As、Pb、Cd、Cr、Sb、Cu、Hg) and identification volatile oil constituents in fragrant ink. Method After microwave digestion,the contents of As,Pb,Cd,Cr,Sb and Cu in fragrant ink were determined by ICP-AES,and Hg was determined by atomic fluorescence spectrometry. The volatile oil samples of fragrant ink were prepared by steam distillation,and the constituents were qualitatively analyzed by GC-MS. Results The methods have good linearity,precision and reproducibility. The recovery after microwave digestion was 86. 0% ～ 104. 37%,RSD was less than5%. Seven kinds of polycyclic aromatic hydrocarbons were identified in volatile oil. Conclusion The contents of heavy metals in fragrant ink were above the limit prescribed in China' s"Green Trade Standards Importing & Exporting Medicinal plants & Preparation".The main components of volatile oil in fragrant ink are polycyclic aromatic hydrocarbons,most of which are carcinogenic and teratogenic.
Objective To establish analysis methods for determination of 7 heavy metals(As、Pb、Cd、Cr、Sb、Cu、Hg) and identification volatile oil constituents in fragrant ink. Method After microwave digestion,the contents of As,Pb,Cd,Cr,Sb and Cu in fragrant ink were determined by ICP-AES,and Hg was determined by atomic fluorescence spectrometry. The volatile oil samples of fragrant ink were prepared by steam distillation,and the constituents were qualitatively analyzed by GC-MS. Results The methods have good linearity,precision and reproducibility. The recovery after microwave digestion was 86. 0% ～ 104. 37%,RSD was less than5%. Seven kinds of polycyclic aromatic hydrocarbons were identified in volatile oil. Conclusion The contents of heavy metals in fragrant ink were above the limit prescribed in China' s"Green Trade Standards Importing & Exporting Medicinal plants & Preparation".The main components of volatile oil in fragrant ink are polycyclic aromatic hydrocarbons,most of which are carcinogenic and teratogenic.
引文
[1]柴瑞霁.京墨止血小考[J].黑龙江中医药,1988,(1):44.
[2]贾敏如,李星炜.中国民族药志[M].北京:中国医药科技出版社,2005:132.
[3]明·李时珍.本草纲目[M].北京:人民卫生出版社,1997:445-446.
[4]清·汪昂.本草备要[M].北京:中国中医药出版社,2008:141.
[5]刘强.京墨治疗带状疱疹[J].湖南中医杂志,1996,11(5):517.
[6]侯新立,张勇.石灰地丁京墨膏治疗带状疱疹28例[J].中国民间疗法,2004,12(1):18.
[7]贾奎寿,郑秀琴.微量元素对人体健康的影响[J].广东微量元素科学,2003,10(1):60-62.
[8]范鹏.重金属对人体健康的影响[J].解放军健康,2004(1):40.
[9]王晓莉,张兆芳,陶翠祥,等.炮制中药古墨的新方法[J].临床合理用药杂志,2012,5(9):74-75.
[10]王伟,方晓阳.中国古代松烟墨制作工艺源流[J].出版与印刷,2010(1):21-25.
[11]符传武,李玲.微波消解ICP-MS法测定壮药美巧杯中5种重金属的含量[J].中国民族民间医药,2014(21):20-22.
[12]陈晋红,汤毅珊,刘大伟,等.姜黄药材中6种重金属残留量测定[J].中药新药与临床药理,2009,20(5):457-460.
[13] WM/T2-2004.《药用植物及制剂进出口绿色行业标准》[S].
[14] GB 2762-2017.《食品中污染物限量》[S].
[15] WHO. Antimony in drinking-water. Background docu-ment of WHO guidelines for drinking-water quality,inantimony in drinking-water[R]. Geneva:WorldHealth Organization,2003.
[16]陆美斌,王步军.不同消解方式测定谷物中八种矿质元素[J].食品工业,2017(9):269-271.
[17]张玉芬,韩娜仁花,赵玉英,等.微波消解-电感耦合等离子体原子发射光谱法测定6种蒙药中7种金属元素[J].中草药,2013(4):434-436.
[18]包奋强,安海龙,惠亚梅,等.硅胶柱与凝胶柱净化/气相色谱-质谱联用测定植物叶片中多环芳烃[J].分析测试学报,2016,35(9):1185-1190.
[19]梅雅楠,李燕,单园园,等.主流烟气中三种稠环芳烃的同时测定[J].山东农业科学,2017,49(6):135-138,150.
[2]贾敏如,李星炜.中国民族药志[M].北京:中国医药科技出版社,2005:132.
[3]明·李时珍.本草纲目[M].北京:人民卫生出版社,1997:445-446.
[4]清·汪昂.本草备要[M].北京:中国中医药出版社,2008:141.
[5]刘强.京墨治疗带状疱疹[J].湖南中医杂志,1996,11(5):517.
[6]侯新立,张勇.石灰地丁京墨膏治疗带状疱疹28例[J].中国民间疗法,2004,12(1):18.
[7]贾奎寿,郑秀琴.微量元素对人体健康的影响[J].广东微量元素科学,2003,10(1):60-62.
[8]范鹏.重金属对人体健康的影响[J].解放军健康,2004(1):40.
[9]王晓莉,张兆芳,陶翠祥,等.炮制中药古墨的新方法[J].临床合理用药杂志,2012,5(9):74-75.
[10]王伟,方晓阳.中国古代松烟墨制作工艺源流[J].出版与印刷,2010(1):21-25.
[11]符传武,李玲.微波消解ICP-MS法测定壮药美巧杯中5种重金属的含量[J].中国民族民间医药,2014(21):20-22.
[12]陈晋红,汤毅珊,刘大伟,等.姜黄药材中6种重金属残留量测定[J].中药新药与临床药理,2009,20(5):457-460.
[13] WM/T2-2004.《药用植物及制剂进出口绿色行业标准》[S].
[14] GB 2762-2017.《食品中污染物限量》[S].
[15] WHO. Antimony in drinking-water. Background docu-ment of WHO guidelines for drinking-water quality,inantimony in drinking-water[R]. Geneva:WorldHealth Organization,2003.
[16]陆美斌,王步军.不同消解方式测定谷物中八种矿质元素[J].食品工业,2017(9):269-271.
[17]张玉芬,韩娜仁花,赵玉英,等.微波消解-电感耦合等离子体原子发射光谱法测定6种蒙药中7种金属元素[J].中草药,2013(4):434-436.
[18]包奋强,安海龙,惠亚梅,等.硅胶柱与凝胶柱净化/气相色谱-质谱联用测定植物叶片中多环芳烃[J].分析测试学报,2016,35(9):1185-1190.
[19]梅雅楠,李燕,单园园,等.主流烟气中三种稠环芳烃的同时测定[J].山东农业科学,2017,49(6):135-138,150.