番茄褪绿病毒在湖南省首次发生
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摘要
2016年7月在湖南省蔬菜病害调查中发现,4种茄科蔬菜表现出叶脉间褪绿、叶片黄化等症状,疑似被番茄褪绿病毒(Tomato chlorosis virus,To CV)感染,同时叶片背面聚集了大量烟粉虱。采用To CV热激蛋白(heat shock protein 70,HSP70)序列的特异性引物对采集的番茄、茄子、辣椒、马铃薯样品和烟粉虱样品进行RT-PCR检测,均扩增出目标条带,且扩增序列与北京番茄To CV分离物(KC887999.1)部分序列相似度为99.0%,确认采集样品被To CV感染。4种茄科蔬菜To CV的感染率达70%~100%;发病叶片上烟粉虱的带毒率为66.7%~87.5%;鉴定出烟粉虱的生物类型为MED烟粉虱。这是湖南省首次确认该病毒,需要引起关注和加强防范。
During the investigation for vegetable diseases of Hunan Province in July 2016,symptoms ofintervein chlorosis and leaf yellowing were discovered on Solanaceae vegetable leaves,including tomato,pepper,eggplant and potato plants. The symptoms were suspected as Tomato chlorosis virus(To CV)and a large number of Bemisia tabaci were found on the back of leaves. The infected leaves of tomato,pepper,eggplant and potato and Bemisia tabaci samples were detected by RT-PCR using gene specific primers of To CV heat shock protein 70(HSP70)sequence,and an expected fragment were obtained. The results of sequence analysis revealed that the fragment shared the highest nucleotide identity at 99.0% with the isolates from Beijing(KC887999.1). The infection rate of To CV of these 4 kinds of Solanaceae vegetables was 70%-100%. Viruliferous rate of Bemisia tabaci was 66.7%-87.5%. The whitefly biotype was MED. This is the first time that Hunan Province confirms the existing of To CV,which needs special attention and stronger prevention.
During the investigation for vegetable diseases of Hunan Province in July 2016,symptoms ofintervein chlorosis and leaf yellowing were discovered on Solanaceae vegetable leaves,including tomato,pepper,eggplant and potato plants. The symptoms were suspected as Tomato chlorosis virus(To CV)and a large number of Bemisia tabaci were found on the back of leaves. The infected leaves of tomato,pepper,eggplant and potato and Bemisia tabaci samples were detected by RT-PCR using gene specific primers of To CV heat shock protein 70(HSP70)sequence,and an expected fragment were obtained. The results of sequence analysis revealed that the fragment shared the highest nucleotide identity at 99.0% with the isolates from Beijing(KC887999.1). The infection rate of To CV of these 4 kinds of Solanaceae vegetables was 70%-100%. Viruliferous rate of Bemisia tabaci was 66.7%-87.5%. The whitefly biotype was MED. This is the first time that Hunan Province confirms the existing of To CV,which needs special attention and stronger prevention.
引文
高利利,孙国珍,王勇,高苇,张春祥,张安胜,竺晓平.2015.天津地区番茄褪绿病毒的分子检测和鉴定.华北农学报,30(3):211-215.
胡京昂,万秀娟,李自娟,黄文,李武高,应芳卿.2015.河南番茄褪绿病毒的分子鉴定.中国蔬菜,(12):25-28.
刘微,史晓斌,唐鑫,张宇,张德咏,周序国,刘勇.2018.云南番茄褪绿病毒和番茄黄化曲叶病毒复合侵染的分子鉴定.园艺学报,45(3):552-560.
刘永光,魏家鹏,乔宁,李美芹,刘晓明,竺晓平.2014.番茄褪绿病毒在山东暴发及其防治措施.中国蔬菜,(5):67-69.
孙国珍,高利利,陆文利,王勇,张安胜,竺晓平.2015.河北省设施番茄褪绿病毒分子检测和鉴定研究.北方园艺,(9):95-98.
汤亚飞,何自福,佘小漫,蓝国兵.2017.侵染广东番茄的番茄褪绿病毒分子鉴定.植物保护,43(2):133-137.
王翠琳,冯佳,孙晓辉,王少立,赵静,刘金亮,竺晓平.2017.北方四省区番茄褪绿病毒的分子鉴定.植物保护,43(2):141-145.
赵黎明.2015.番茄褪绿病毒的分子鉴定、全基因组序列分析及检测技术的建立[硕士论文].泰安:山东农业大学.
郑慧新,夏吉星,周小毛,张友军.2016.警惕烟粉虱传播的番茄褪绿病毒病在我国快速扩散.中国蔬菜,(4):22-26.
周涛,杨普云,赵汝娜,师迎春,原锴,范在丰.2014.警惕番茄褪绿病毒在我国的传播和危害.植物保护,(5):196-199.
Chu D,Zhang Y J,Brown J K,Cong B,Xu B Y,Wu Q J,Zhu G R.2006.The introduction of the exotic Q biotype of Bemisia tabaci from the Mediterranean region into China on ornamental crops.Florida Entomologist,89(2):168-174.
Chu D,Wan F H,Zhang Y J,Brown J K.2010.Change in the biotype composition of Bemisia tabaci in Shandong province of China from 2005 to 2008.Environmental Entomology,39(3):1028-1036.
Landeo-Ríos Y M,Navas-Castillo J,Moriones E,Ca?izares M C.2015.Genetic diversity and silencing suppression activity of the p22protein of Tomato chlorosis virus,isolates from tomato and sweet pepper.Virus Genes,51(2):283-289.
Orfanidou C,Pappi P G,Efthimiou K E,Katis N,Maliogka V I.2016.Transmission of Tomato chlorosis virus(To CV)by Bemisia tabaci biotype Q and evaluation of four weed species as viral sources.Plant Disease,100(10):10.1094/PDIS-01-16-0054-RE.
Shi X,Tang X,Zhang X,Zhang D Y,Li F,Yan F,Zhang Y J,Zhou X G,Liu Y.2017.Transmission efficiency,preference and behavior of Bemisia tabaci MEAM1 and MED under the influence of Tomato chlorosis virus.Frontiers in Plant Science,8:2271-2280.
Tang X,Shi X,Zhang D,Li F,Yan F,Zhang Y,Liu Y,Zhou X.2017.Detection and epidemic dynamic of To CV and CCYV with Bemisia tabaci and weed in Hainan of China.Virology Journal,14:doi:10.1186/s12985-017-0833-2.
Tsai W S,Shih S L,Green S K,Hanson P.2004.First report of the occurrence of Tomato chlorosis virus and Tomato infectious chlorosis virus in Taiwan.Plant Disease,88(3):311.
Wintermantel W M,Wisler G C.2006.Vector specificity,host range,and genetic diversity of Tomato chlorosis virus.Plant Disease,90(6):814-819.
Wisler G C,Li R H,Liu H Y,Lowry D S,Duffus J E.1998.Tomato chlorosis virus:a new whitefly-transmitted,phloem-limited,bipartite closterovirus of tomato.Phytopathology,88(5):402-409.
Xi T,Wan F H,Dong C.2010.Bemisia tabaci biotype Q dominates other biotypes across China.Florida Entomologist,93(3):363-368.
Xin L,Zhang Y,Wen X,Wu Q,Wang S.2016.The suitability of biotypes Q and B of Bemisia tabaci(Gennadius)(Hemiptera:Aleyrodidae)at different nymphal instars as hosts for Encarsia formosa Gahan(Hymenoptera:Aphelinidae).Peerj,4(4):e1863.
Zhao R N,Wang R,Wang N,Fan Z F,Zhou T,Shi Y C,Zhou T.2013.First report of Tomato chlorosis virus in China.Plant Disease,97(8):1123.
胡京昂,万秀娟,李自娟,黄文,李武高,应芳卿.2015.河南番茄褪绿病毒的分子鉴定.中国蔬菜,(12):25-28.
刘微,史晓斌,唐鑫,张宇,张德咏,周序国,刘勇.2018.云南番茄褪绿病毒和番茄黄化曲叶病毒复合侵染的分子鉴定.园艺学报,45(3):552-560.
刘永光,魏家鹏,乔宁,李美芹,刘晓明,竺晓平.2014.番茄褪绿病毒在山东暴发及其防治措施.中国蔬菜,(5):67-69.
孙国珍,高利利,陆文利,王勇,张安胜,竺晓平.2015.河北省设施番茄褪绿病毒分子检测和鉴定研究.北方园艺,(9):95-98.
汤亚飞,何自福,佘小漫,蓝国兵.2017.侵染广东番茄的番茄褪绿病毒分子鉴定.植物保护,43(2):133-137.
王翠琳,冯佳,孙晓辉,王少立,赵静,刘金亮,竺晓平.2017.北方四省区番茄褪绿病毒的分子鉴定.植物保护,43(2):141-145.
赵黎明.2015.番茄褪绿病毒的分子鉴定、全基因组序列分析及检测技术的建立[硕士论文].泰安:山东农业大学.
郑慧新,夏吉星,周小毛,张友军.2016.警惕烟粉虱传播的番茄褪绿病毒病在我国快速扩散.中国蔬菜,(4):22-26.
周涛,杨普云,赵汝娜,师迎春,原锴,范在丰.2014.警惕番茄褪绿病毒在我国的传播和危害.植物保护,(5):196-199.
Chu D,Zhang Y J,Brown J K,Cong B,Xu B Y,Wu Q J,Zhu G R.2006.The introduction of the exotic Q biotype of Bemisia tabaci from the Mediterranean region into China on ornamental crops.Florida Entomologist,89(2):168-174.
Chu D,Wan F H,Zhang Y J,Brown J K.2010.Change in the biotype composition of Bemisia tabaci in Shandong province of China from 2005 to 2008.Environmental Entomology,39(3):1028-1036.
Landeo-Ríos Y M,Navas-Castillo J,Moriones E,Ca?izares M C.2015.Genetic diversity and silencing suppression activity of the p22protein of Tomato chlorosis virus,isolates from tomato and sweet pepper.Virus Genes,51(2):283-289.
Orfanidou C,Pappi P G,Efthimiou K E,Katis N,Maliogka V I.2016.Transmission of Tomato chlorosis virus(To CV)by Bemisia tabaci biotype Q and evaluation of four weed species as viral sources.Plant Disease,100(10):10.1094/PDIS-01-16-0054-RE.
Shi X,Tang X,Zhang X,Zhang D Y,Li F,Yan F,Zhang Y J,Zhou X G,Liu Y.2017.Transmission efficiency,preference and behavior of Bemisia tabaci MEAM1 and MED under the influence of Tomato chlorosis virus.Frontiers in Plant Science,8:2271-2280.
Tang X,Shi X,Zhang D,Li F,Yan F,Zhang Y,Liu Y,Zhou X.2017.Detection and epidemic dynamic of To CV and CCYV with Bemisia tabaci and weed in Hainan of China.Virology Journal,14:doi:10.1186/s12985-017-0833-2.
Tsai W S,Shih S L,Green S K,Hanson P.2004.First report of the occurrence of Tomato chlorosis virus and Tomato infectious chlorosis virus in Taiwan.Plant Disease,88(3):311.
Wintermantel W M,Wisler G C.2006.Vector specificity,host range,and genetic diversity of Tomato chlorosis virus.Plant Disease,90(6):814-819.
Wisler G C,Li R H,Liu H Y,Lowry D S,Duffus J E.1998.Tomato chlorosis virus:a new whitefly-transmitted,phloem-limited,bipartite closterovirus of tomato.Phytopathology,88(5):402-409.
Xi T,Wan F H,Dong C.2010.Bemisia tabaci biotype Q dominates other biotypes across China.Florida Entomologist,93(3):363-368.
Xin L,Zhang Y,Wen X,Wu Q,Wang S.2016.The suitability of biotypes Q and B of Bemisia tabaci(Gennadius)(Hemiptera:Aleyrodidae)at different nymphal instars as hosts for Encarsia formosa Gahan(Hymenoptera:Aphelinidae).Peerj,4(4):e1863.
Zhao R N,Wang R,Wang N,Fan Z F,Zhou T,Shi Y C,Zhou T.2013.First report of Tomato chlorosis virus in China.Plant Disease,97(8):1123.