响应面法优化阳离子胶原蛋白絮凝剂的制备条件
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摘要
以皮革废弃物提取的明胶为原料,水解后得到胶原蛋白水解液,以叔丁基过氧化氢和焦亚硫酸钠为引发剂,与阳离子单体丙烯酰氧乙基三甲基氯化铵(DAC)接枝共聚,制备了阳离子胶原蛋白絮凝剂PCDAC,并用于废弃钻井液的絮凝处理中。通过响应面法优化了PCDAC制备过程中明胶的最佳水解条件:复合酶(以明胶的质量计,中性蛋白酶与胰蛋白酶质量比为1∶1)用量3%,水解pH值7. 0,水解温度38℃,水解时间1. 85h。在此条件下,PCDAC对废弃钻井液悬浮物的去除率为(69. 08±0. 32)%。
Collagen hydrolysate was prepared from gelatin which was extracted from leather solid wastes. Cationic collagen flocculant (PCDAC) was synthesized by graft copolymerization using the collagen hydrolysate and acryloyl oxyethyl trimethyl ammonium chloride (DAC),the synthesis reaction was initiated with tert buty hydroperoxide and focal sulfite sodium. The effects of PCDAC on harmless treatment of waste drilling fluid were studied. Gelatin hydrolysis conditions were optimized in PCDAC preparation by response surface methodology,the optimum conditions are as follows: compound enzyme dosage (the ratio of neutral protease and trypsin is 1:1) 3%,p H value 7. 0,temperature38℃,reaction time 1. 85 h. Under these conditions,the suspended substance removal rate of waste drilling fluid is (69. 08±0. 32) %with PCDAC.
Collagen hydrolysate was prepared from gelatin which was extracted from leather solid wastes. Cationic collagen flocculant (PCDAC) was synthesized by graft copolymerization using the collagen hydrolysate and acryloyl oxyethyl trimethyl ammonium chloride (DAC),the synthesis reaction was initiated with tert buty hydroperoxide and focal sulfite sodium. The effects of PCDAC on harmless treatment of waste drilling fluid were studied. Gelatin hydrolysis conditions were optimized in PCDAC preparation by response surface methodology,the optimum conditions are as follows: compound enzyme dosage (the ratio of neutral protease and trypsin is 1:1) 3%,p H value 7. 0,temperature38℃,reaction time 1. 85 h. Under these conditions,the suspended substance removal rate of waste drilling fluid is (69. 08±0. 32) %with PCDAC.
引文
[1]马骉,蒲晓林,张舒.废弃钻井液处理技术研究进展及发展趋势[J].现代化工,2017(4):42-45,47.
[2]王学川,胡艳鑫,郑书杰,等.国内外废弃钻井液处理技术研究现状[J].陕西科技大学学报(自然科学版),2010,28(6):169-174.
[3]王中华.国内钻井液处理剂研发现状与发展趋势[J].石油钻探技术,2016(3):1-8.图11水解温度和水解时间交互作用对悬浮物去除率的影响Fig.11 The interaction influence of hydrolysis temperature and hydrolysis time on the removal rate of suspended matter
[4]陈琼,赵长征,梁宏.钻井废水处理技术及展望[J].油气田环境保护,2008,18(2):49-52.
[5]郑立稳,张闻,孔学,等.废弃钻井液处理技术研究[J].油气田环境保护,2017,17(2):11-13,60.
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[9]张月娥.制革固体废弃物的综合利用[D].天津:天津科技大学,2013.
[10]王学川,张莎,周亮,等.胶原蛋白改性阳离子絮凝剂对废弃钻井液的絮凝[J].钻井液与完井液,2012,29(2):73-75,95-96.
[11]王学川,潘博,强涛涛,等.阳离子胶原蛋白絮凝剂改善污泥脱水性能的研究[J].工业水处理,2015,35(7):19-23.
[12]Brown E M,Taylor M M,Marmer W N.Production and potential uses of co-products from solid tannery waste[J].JALCA,1996,91(10):270-276.
[13]Zhang Y,Miao Z,Zou J.A new cation-modified Al-polyacrylamide flocculant for solid-liquid separation in waste drilling fluid[J].Journal of Applied Polymer Science,2015,132(11):41 641-41 649.
[14]Ganjidoust H,Tatsumi K,Yamagishi T,et al.Effect of synthetic and natural coagulant on lignin removal from pulp and paper waste-water[J].Water Science and Technology,1997,35(2/3):291-296.
[2]王学川,胡艳鑫,郑书杰,等.国内外废弃钻井液处理技术研究现状[J].陕西科技大学学报(自然科学版),2010,28(6):169-174.
[3]王中华.国内钻井液处理剂研发现状与发展趋势[J].石油钻探技术,2016(3):1-8.图11水解温度和水解时间交互作用对悬浮物去除率的影响Fig.11 The interaction influence of hydrolysis temperature and hydrolysis time on the removal rate of suspended matter
[4]陈琼,赵长征,梁宏.钻井废水处理技术及展望[J].油气田环境保护,2008,18(2):49-52.
[5]郑立稳,张闻,孔学,等.废弃钻井液处理技术研究[J].油气田环境保护,2017,17(2):11-13,60.
[6]周静华.油田钻井废弃钻井液的处理技术[J].石化技术,2015(8):155.
[7]吕凌云,马兴元,蒋坤,等.从皮革废弃物中提取胶原蛋白及其高值化应用的研究进展[J].西部皮革,2010,32(15):46-51.
[8]王睿,向旭,黄彬,等.制鞋业废弃物回收利用研究进展(续)[J].中国皮革,2017,46(5):41-44,49.
[9]张月娥.制革固体废弃物的综合利用[D].天津:天津科技大学,2013.
[10]王学川,张莎,周亮,等.胶原蛋白改性阳离子絮凝剂对废弃钻井液的絮凝[J].钻井液与完井液,2012,29(2):73-75,95-96.
[11]王学川,潘博,强涛涛,等.阳离子胶原蛋白絮凝剂改善污泥脱水性能的研究[J].工业水处理,2015,35(7):19-23.
[12]Brown E M,Taylor M M,Marmer W N.Production and potential uses of co-products from solid tannery waste[J].JALCA,1996,91(10):270-276.
[13]Zhang Y,Miao Z,Zou J.A new cation-modified Al-polyacrylamide flocculant for solid-liquid separation in waste drilling fluid[J].Journal of Applied Polymer Science,2015,132(11):41 641-41 649.
[14]Ganjidoust H,Tatsumi K,Yamagishi T,et al.Effect of synthetic and natural coagulant on lignin removal from pulp and paper waste-water[J].Water Science and Technology,1997,35(2/3):291-296.