干旱胁迫对风箱果幼苗生长、光合生理和抗氧化酶活性的影响
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摘要
以1年生风箱果幼苗为材料,设置对照(CK)、轻度干旱胁迫(W1)、中度干旱胁迫(W2)和重度干旱胁迫(W3)等4种处理,研究不同干旱胁迫对幼苗的生长表现、生物量积累、光合生理特征和抗氧化酶活性等的影响。结果表明:W1处理对幼苗的生长和生物量积累影响较小,W2处理和W3处理显著抑制了幼苗的生长和生物量的积累,特别是W3处理,苗高和地径比CK分别减少了71.4%和77.8%,根、茎、叶和总生物量比CK分别减少了33.3%、75.0%、69.2%和57.6%;光合生理参数和抗氧化酶活性均随干旱胁迫加剧呈先增大后减小的趋势,W1处理最大;W1处理对丙二醛(MDA)质量摩尔浓度和细胞膜相对透性影响较小,W2处理和W3处理影响显著增大。风箱果幼苗能够适应轻度干旱胁迫,中、重度胁迫下生长和光合能力均受到抑制,抗氧化酶系统遭到破坏。
One-year-old Physocarpus amurensis seedlings were grown in pots to study the effects of different drought stress on seedling growth performance,biomass accumulation,photosynthetic physiological characteristics and antioxidant enzyme activities with four kinds of stress treatments including control( CK),light drought stress( W1),moderate drought stress(W2) and heavy drought stress(W3). W1 had little effect on seedling growth and biomass accumulation. W2 and W3 significantly inhibited seedling growth and biomass accumulation. Especially,W3,seedling height and ground diameter decreased by 71.4% and 77. 8%,respectively,compared with CK,and root,stem,leaf and total biomass decreased by33.3%,75.0%,69.2% and 57.6%,respectively,compared with CK. Photosynthetic physiological parameters and antioxidant enzymes activities increased and then decreased with the drought stress aggravated,and reached the maximum at W1.W1 had little effect on the contents of MDA and membrane permeability,and increased significantly at W2 and W3. P.amurensis seedlings were able to adapt to light drought stress,the growth and photosynthetic ability of the seedlings were inhibited,and the antioxidant enzyme system was destroyed under moderate and heavy drought stress.
One-year-old Physocarpus amurensis seedlings were grown in pots to study the effects of different drought stress on seedling growth performance,biomass accumulation,photosynthetic physiological characteristics and antioxidant enzyme activities with four kinds of stress treatments including control( CK),light drought stress( W1),moderate drought stress(W2) and heavy drought stress(W3). W1 had little effect on seedling growth and biomass accumulation. W2 and W3 significantly inhibited seedling growth and biomass accumulation. Especially,W3,seedling height and ground diameter decreased by 71.4% and 77. 8%,respectively,compared with CK,and root,stem,leaf and total biomass decreased by33.3%,75.0%,69.2% and 57.6%,respectively,compared with CK. Photosynthetic physiological parameters and antioxidant enzymes activities increased and then decreased with the drought stress aggravated,and reached the maximum at W1.W1 had little effect on the contents of MDA and membrane permeability,and increased significantly at W2 and W3. P.amurensis seedlings were able to adapt to light drought stress,the growth and photosynthetic ability of the seedlings were inhibited,and the antioxidant enzyme system was destroyed under moderate and heavy drought stress.
引文
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[20] GOKMEN E,CEYHAN E. Effcets of drought stress on growth parameters,enzyme activities and proline content in chickpea genotupes[J]. Bangladesh Journal of Botany,2015,44(2):177-183.
[21]曹兵,苏润海,王标,等.水分胁迫下臭椿幼苗几个生理指标的变化[J].林业科技,2003,28(3):1-3.
[22] SOHRABI Y,HEIDARI G,WEISANY W,et al. Changes of antioxidative enzymes,lipid peroxidation and chlorophyll content in chickpea types colonized by different Glomus species under drought stress[J]. Symbiosis,2012,56(1):5-18.
[2] BENEOVM,HOLD,FISCHER L,et al. The physiology and proteomics of drought tolerance in maize:early stomatal closure as a cause of lower tolerance to shortterm dehydration[J]. PLoS One,2012,7(6).doi:10.1371/journal.pone.0038017.
[3]柴春荣,穆立蔷,梁鸣,等.北方6种绿化灌木水分胁迫的生理响应[J].东北林业大学学报,2012,40(6):12-15.
[4]殷东生,沈海龙,魏晓慧.遮荫对风箱果幼苗光合能力和碳水化合物积累的影响[J].植物研究,2017,37(6):841-847.
[5]范苏鲁,苑兆和,冯立娟,等.水分胁迫对大丽花光合作用、蒸腾和气孔导度的影响[J].中国农学通报,2011,27(8):119-122.
[6]裴斌,张光灿,张淑勇,等.土壤干旱胁迫对沙棘叶片光合作用和抗氧化酶活性的影响[J].生态学报,2013,33(5):1386-1396.
[7]邹琦.植物生理学实验指导[M].北京:中国农业出版社,2000.
[8] MARAGHNI M,GORAI M,NEFFATI M,et al. Differential responses to drought stress in leaves and roots of wild jujube,Ziziphus lotus[J]. Acta Physiologiae Plantarum,2014,36(4):945-953.
[9] BENJAMIN J G,NIELSEN D C,VIGIL H F,et al. Water deficit stress effects on corn(Zea mays L.)root:shootratio[J]. Open Journal of Soil Science,2014,4(4):151-160.
[10] LOPES M S,ARAUS J L,VAN HEERDEN P D,et al. Enhancing drought tolerance in C4 crops[J]. Journal of Experimental Botany,2011,62(9):3135-3153.
[11] FENTA B A,DRISCOLL S P,KUNERT K J,et al. Characterization of drought-tolerance traits in nodulated soya beans:the importance of maintaining photosynthesis and shoot biomass under drought-induced limitations on nitrogen metabolism[J]. Journal of Agronomy&Crop Science,2012,198(2):92-103.
[12] XIANG D B,PENG L X,ZHAO J L,et al. Effect of drought stress on yield,chlorophyll contents and photosynthesis in tartary buckwheat(Fagopyrum tataricum)[J]. Journal of Food Agriculture&Environment,2013,11(3):1358-1363.
[13] GHOTBI-RAVANDI A A,SHAHBAZI M,SHARIATI M,et al.Effects of mild and severe drought stress on photosynthetic efficiency in tolerant and susceptible barley(Hordeum vulgare L.)genotypes[J]. Journal of Agronomy&Crop Science,2015,200(6):403-415.
[14] FARQUHAR G D,SHARKEY T D. Stomatal conductance and photosynthesis[J]. Annual Reviews of Plant Physiology,1982,33:317-345.
[15]户连荣,郎南军,郑科.植物抗旱性研究进展及发展趋势[J].安徽农业科学,2008,36(7):2652-2654.
[16] STEDUTO P,KATERJI N,PUERTOS-MOLINA H,et al. Water-use efficiency of sweet sorghum under water stress conditions:Gas-exchange investigations at leaf and canopy scales[J]. Field Crops Research,1997,54(2/3):221-234.
[17] NOCTOR G,FOYER C H. Ascorbate and glutathione:keeping active oxygen under control[J]. Annual Review of Plant Physiology&Plant Molecular Biology,1998,49(49):249-279.
[18]史小玲,薛立,任向荣,等.华南地区4种阔叶幼苗水分胁迫条件下的抗旱性初探[J].林业科学研究,2011,24(6):760-767.
[19]黎燕琼,郑绍伟,陈泓,等.林木抗旱性研究及其进展[J].世界林业研究,2007,20(1):10-15.
[20] GOKMEN E,CEYHAN E. Effcets of drought stress on growth parameters,enzyme activities and proline content in chickpea genotupes[J]. Bangladesh Journal of Botany,2015,44(2):177-183.
[21]曹兵,苏润海,王标,等.水分胁迫下臭椿幼苗几个生理指标的变化[J].林业科技,2003,28(3):1-3.
[22] SOHRABI Y,HEIDARI G,WEISANY W,et al. Changes of antioxidative enzymes,lipid peroxidation and chlorophyll content in chickpea types colonized by different Glomus species under drought stress[J]. Symbiosis,2012,56(1):5-18.