摘要
目的:观察龙牡桂枝汤对多动症模型大鼠左右前额叶皮质和纹状体脑源性神经营养因子(BDNF)、胶质细胞源性神经营养因子(GDNF) mRNA表达的影响。方法:幼年雄性SHR大鼠随机分为龙牡桂枝汤组、盐酸哌甲酯组和生理盐水组,分别予相应药物灌胃14d,采用实时荧光定量PCR法检测大鼠左侧前额叶皮质和纹状体BDNF、GDNF mRNA的表达水平。结果:组内比较:生理盐水组SHR大鼠左侧前额叶皮质BDNF和GDNF mRNA表达较右侧显著升高(P<0.05),而左侧纹状体BDNF和GDNF mRNA表达较右侧显著降低(P<0.05)。组间比较:两用药组左侧前额叶皮质BDNF mRNA表达较生理盐水组显著降低(P<0.01),两用药组右侧纹状体BDNF mRNA表达较生理盐水组显著升高(P<0.05)。龙牡桂枝汤组左纹状体GDNF mRNA表达较生理盐水组显著升高(P<0.05)。结论:SHR大鼠左右前额叶皮质和纹状体BDNF、GDNF mRNA表达存在差异,龙牡桂枝汤治疗多动症的作用机制可能与调节左右前额叶皮质和纹状体BDNF mRNA及左侧纹状体GDNF mRNA表达有关。
Objective: To study effects of Longmu Guizhi Decoction on the mRNA expression of BDNF and GDNF in the left and right prefrontal cortex and striatum of spontaneously hypertensive rats(SHR) with hyperactivity disorder. Methods:Juvenile male SHR rats were randomly divided into Longmu Guizhi Decoction group(LD), methylphenidate control group and saline control group. The rats received gastric gavage of corresponding drugs for 14 days. The Real-time PCR method was used to test the BDNF and GDNF mRNA expressions in the left and right prefrontal cortexand striatum. Results: Self comparison:in saline control group, BDNF and GDNF mRNA expression of the left prefrontal cortex were significantly higher than the right(P<0.05), but the expression of BDNF and GDNF mRNA in the left striatum was lower than that in the right(P<0.05),Comparison among groups: BDNF mRNA expression in the left prefrontal cortex of methylphenidate control group and LD group were significantly lower than the saline control group(P<0.01), BDNF mRNA expression in the right striatum of methylphenidate control group and LD group were significantly higher than the saline control group(P<0.05), GDNF mRNA expression in the left striatum of LD group were significantly higher than the saline control group(P<0.05). Conclusion: BDNF and GDNF mRNA expression of SHR rats were different in the left and right prefrontal cortex and striatum. The potential mechanism of LD in the treatment of ADHD is related to the regulation of the BDNF mRNA expression in the left and right prefrontal cortex and striatum,as well as GDNF mRNA expression in the left striatum.
引文
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