乌蒙凤鸡凤头性状与Hoxc8基因表达及其生长性能的相关研究
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摘要
同源盒基因C8 (homeobox gene C8, Hoxc8)对鸡(Gallus gallus)凤头性状有着重要调节作用,且凤头性状对鸡早期生长性能有显著影响。为研究乌蒙凤鸡Hoxc8基因表达量与凤头性状及其生长性能的相关性,本研究以乌蒙凤鸡为研究对象,测定不同凤头性状乌蒙凤鸡早期生长性能及Hoxc8基因表达量。研究结果显示,Hoxc8基因在成年有凤头乌蒙凤鸡各组织中均有表达,在凤头骨中表达量最高,在睾丸中表达量较高,在肾、胸肌、腿肌、胫骨和大肠中表达量居中,在心、肝、卵巢、脾脏中表达量较低(P<0.01);在24周龄有凤头乌蒙凤鸡头皮和背皮组织中,Hoxc8基因的表达量极显著低于无凤头乌蒙凤鸡(P<0.01);Hoxc8基因在不同性别乌蒙凤鸡各组织中呈差异表达:在24周龄乌蒙凤鸡中,公鸡头皮、背皮组织中表达量极显著高于母鸡(P<0.01);在成年有凤头乌蒙凤鸡中,公鸡胫骨、腿肌组织表达量高于母鸡,母鸡心、肝、下丘脑、垂体组织表达量高于公鸡;凤头性状对乌蒙凤鸡早期生长性能有影响,随着周龄的增长,有凤头乌蒙凤鸡体重、体斜长、胸深、胸宽、胫长指标生长趋势较无凤头乌蒙凤鸡好;有凤头和无凤头乌蒙凤鸡公鸡早期生长性能优于母鸡;出壳重较大的乌蒙凤鸡早期增重较好。上述研究结果表明,乌蒙凤鸡凤头性状与Hoxc8基因头皮、背皮组织特异表达有关,有凤头乌蒙凤鸡早期生长性能较好。本研究结果对乌蒙凤鸡的保种选育有一定指导意义。
Many studies have shown that homeobox C8(Hoxc8) gene plays an important regulatory role in the crested traits of chickens(Gallus gallus), and the crested traits have a significant impact on the early growth performance of the chickens. Therefore, in order to study the correlation between the expression of Hoxc8 gene and the crested traits and growth performance of Wumen crested chickens, this study had taken the Wumeng crested chickens as research objects, measuring the early growth performance and the expression of Hoxc8 gene in different tissues. The results showed that Hoxc8 gene was expressed in all tissues of adult crested Wumeng crested chickens. It had the highest expression in crested bone; higher expression in testis;moderate expression in kidney, chest muscle, leg muscle, tibia and large intestine; and lower expression in heart, liver, ovary, hypothalamus spleen(P<0.01); Hoxc8 gene was differentially expressed in the scalp and back shin tissues of Wumeng crested chickens with 24 weeks of age, with the slight expression in crested chickens and higher expression in non-crested chickens(P<0.01). Hoxc8 gene was differentially expressed in the tissues of female and male Wumeng crested chickens: The expression of scalp and back shin tissue in cocks of 24 weeks old Wumeng crested chickens were significantly higher than that of hens(P<0.01). In adult crested Wumeng crested chickens, the expression of tibia and leg muscle tissue of cocks was higher than that of hens, and the expression of heart, liver, hypothalamus and pituitary tissue of hens were higher than that of cocks; The crested traits had an effect on the early growth performance of Wumeng crested chickens. With the increase of the age of the week, the growth trend of the body weight, body length, chest depth, chest width and tibia length of crested Wumeng crested chickens were better than that of the non-crested Wumeng crested chickens; The early growth performance of cocks with crested and non-crested Wumeng crested chickens were better than that of hens; the early weight gain of Wumeng crested chickens with better shelling weight was better. The above results showed that the crested traits of Wumeng crested chickens were related to the specific expression of Hoxc8 gene in their scalp and back skin tissue. there was a good growth performance of crested Wumeng crested chickens. The results of this study could provide certain guiding significant for the conservation and breeding of Wumeng crested chickens.
Many studies have shown that homeobox C8(Hoxc8) gene plays an important regulatory role in the crested traits of chickens(Gallus gallus), and the crested traits have a significant impact on the early growth performance of the chickens. Therefore, in order to study the correlation between the expression of Hoxc8 gene and the crested traits and growth performance of Wumen crested chickens, this study had taken the Wumeng crested chickens as research objects, measuring the early growth performance and the expression of Hoxc8 gene in different tissues. The results showed that Hoxc8 gene was expressed in all tissues of adult crested Wumeng crested chickens. It had the highest expression in crested bone; higher expression in testis;moderate expression in kidney, chest muscle, leg muscle, tibia and large intestine; and lower expression in heart, liver, ovary, hypothalamus spleen(P<0.01); Hoxc8 gene was differentially expressed in the scalp and back shin tissues of Wumeng crested chickens with 24 weeks of age, with the slight expression in crested chickens and higher expression in non-crested chickens(P<0.01). Hoxc8 gene was differentially expressed in the tissues of female and male Wumeng crested chickens: The expression of scalp and back shin tissue in cocks of 24 weeks old Wumeng crested chickens were significantly higher than that of hens(P<0.01). In adult crested Wumeng crested chickens, the expression of tibia and leg muscle tissue of cocks was higher than that of hens, and the expression of heart, liver, hypothalamus and pituitary tissue of hens were higher than that of cocks; The crested traits had an effect on the early growth performance of Wumeng crested chickens. With the increase of the age of the week, the growth trend of the body weight, body length, chest depth, chest width and tibia length of crested Wumeng crested chickens were better than that of the non-crested Wumeng crested chickens; The early growth performance of cocks with crested and non-crested Wumeng crested chickens were better than that of hens; the early weight gain of Wumeng crested chickens with better shelling weight was better. The above results showed that the crested traits of Wumeng crested chickens were related to the specific expression of Hoxc8 gene in their scalp and back skin tissue. there was a good growth performance of crested Wumeng crested chickens. The results of this study could provide certain guiding significant for the conservation and breeding of Wumeng crested chickens.
引文
金淑清,浦予飞,裘莹.2011.HOX基因的研究进展[J].癌症进展,09(2):154-158.(Jin S Q,Pu Y F,X Y.2011.Progress in research on HOX gene[J].Oncology Progress,09(2):154-158.)
裴勤娴.2007.鸡GH基因和GHR基因多态性与早期生长性能的相关性研究[D].硕士学位论文,贵州大学,导师:陈彬,pp.34-41.(Pei Q X.2007.Correlation GH gene and GHR gene between its polymorphism and early growth performance of chickens[D].Thesis for M.S.,Guizhou University,Supervisor:Chen B,pp.34-41.)
王彦强,顾晓荣,冯春刚,等.2012.与鸡缨头性状相关的Hoxc8基因及应用中国,102094022[P].(Wang Y Q,Gu X R,Feng C G,et al.Hoxc8 gene associated with chicken crest traits and its application.China,102094022[P].)
阳光远,张小林,李元佳.2012.百宜黑鸡生长曲线拟合与分析比较研究[J].畜牧与兽医,44(8):37-39.(Yang G Y,Zhang X L,Li Y J.2012.Study with growth curve fitting and comparative analysis of Baiyi black chicken[J].Animal Husbandry and Veterinary Medicine,44(8):37-39.)
朱远春,查龙应,傅筑荫.2004.贵州兴义矮脚鸡与矮脚黄鸡早期生长及屠宰性能的比较[J].贵州农业科学,32(3):19-21.(Zhu Y C,Cha L Y,Fu Z Y.2004.Study on the early growth and dressed characters in Bantam Chicken[J].Guizhou Agricultural Sciences,32(3):19-21.)
王彬.2015.凤头白鸭与连城白鸭生产性能、遗传结构和部分凤头性状相关基因比较[D].硕士学位论文,扬州大学,导师:陈国宏.pp.39-42(Wang B.2015.Comparision of productivity,hereditary constitution and the expression of certain crest phenotype related genes between crested white duck and Liancheng white ducks[D].Thesis for M.S.,Yangzhou University,Supervisor:Chen G H,pp.39-42.)
赵燕,陈黎,卢立志.2014.家禽缨头性状及其候选基因研究进展[J].畜牧与兽医,46(4):100-102.(Zhao Y,Chen L,Lu L Z.2014.Research progress of poultry crest traits and their candidate genes[J].Animal Husbandry&Veterinary Medicine,46(4):100-102.)
郑艳军.2009.HoxC8基因在小鼠成骨细胞分化中的调控作用研究[D].博士学位论文,吉林大学,导师:朱平,pp.43-83.(Zheng Y Z.2009.Regulation rules of HoxC8 in mouse osteoblast differentiation[D].Thesis for Ph.D.,Ji Lin University,Supervisor:Zhu P,pp.43-83.)
Belting H G,Shashikant C S,Ruddle F H.1998.Modification of expression and cis-regulation of Hoxc8 in the evolution of diverged axial morphology[J].Proceedings of the National Academy of Sciences of the USA,95(5):2355.
Frahm H D,Rehk?mper G.1998.Allometric comparison of the brain and brain structures in the white crested polish chicken with uncrested domestic chicken breeds.[J].Brain,Behavior and Evolution,52(6):292-307.
Romanov M N,Sazanov A A,Smirnov A F.2004.First century of chicken gene study and mapping-a look back and forward[J].Worlds Poultry Science Journal,60(1):19-41.
Shi X,Yang X,Chen D,et al.1999.Smad1 interacts with homeobox DNA-binding proteins in bone morphogenetic protein signaling[J].Journal of Biological Chemistry,274(19):13711-13717.
Huang S,Li Y.2015.HOXC8(homeobox C8)[J].Atlas Genet Cytogenet Oncol Haematol.19(4):266-269.
Joller S,Ammann P,Flury C,et al.2018.Evaluation of HOXC8 in crested Swiss chicken[J].Animal Genetics,49(4):334-336.
Kruger C,Kappen C.2010.Expression of cartilage developmental genes in Hoxc8-and Hoxd4-transgenic mice[J].Plos One,5(2):e8978.
Lei H,Juan A H,Kim M S,et al.2006.Identification of a Hoxc8-regulated transcriptional network in mouse embryo fibroblast cells[J].Proceedings of the National Academy of Sciences of the USA,103(27):10305-10309.
Liu Z.2004.Molecules mimicking Smad1 interacting with hox stimulate bone for mation[J].Journal of Biological Chemistry,279(12):11313-11319.
Li X,Nie S,Chang C,et al.2006.Smads oppose Hox transcriptional activities[J].Experimental Cell Research,312(6):854-864.
Magothe T M,Muhuyi W B,Kahi A K.2010.Influence of major genes for crested-head,frizzle-feather and nakedneck on body weights and growth patterns of indigenous chickens reared intensively in Kenya[J].Tropical Animal Health&Production,42(2):173.
Sun Y,Liu R,Zhao G,et al.2015.Genome-wide linkage analysis identifies loci for physical appearance traits in chickens[J].G3 Genesgenetics,5(10):2037-2041.
Yueh Y G,Kappen G C.1998.Evidence for regulation of cartilage differentiation by the homeobox gene Hoxc-8[J].Proceedings of the National Academy of Sciences of the USA,95(17):9956-9961.
Wang Y,Gao Y,Imsland F,et al.2012.The Crest phenotype in chicken is associated with ectopic expression of,HOXC8,in cranial skin[J].Plos One,7(4):e34012.
Zheng Y J,Chung H J,Min H,et al.2010.In vitro osteoblast differentiation is negatively regulated by Hoxc8[J].Applied Biochemistry&Biotechnology,160(3):891-900.
裴勤娴.2007.鸡GH基因和GHR基因多态性与早期生长性能的相关性研究[D].硕士学位论文,贵州大学,导师:陈彬,pp.34-41.(Pei Q X.2007.Correlation GH gene and GHR gene between its polymorphism and early growth performance of chickens[D].Thesis for M.S.,Guizhou University,Supervisor:Chen B,pp.34-41.)
王彦强,顾晓荣,冯春刚,等.2012.与鸡缨头性状相关的Hoxc8基因及应用中国,102094022[P].(Wang Y Q,Gu X R,Feng C G,et al.Hoxc8 gene associated with chicken crest traits and its application.China,102094022[P].)
阳光远,张小林,李元佳.2012.百宜黑鸡生长曲线拟合与分析比较研究[J].畜牧与兽医,44(8):37-39.(Yang G Y,Zhang X L,Li Y J.2012.Study with growth curve fitting and comparative analysis of Baiyi black chicken[J].Animal Husbandry and Veterinary Medicine,44(8):37-39.)
朱远春,查龙应,傅筑荫.2004.贵州兴义矮脚鸡与矮脚黄鸡早期生长及屠宰性能的比较[J].贵州农业科学,32(3):19-21.(Zhu Y C,Cha L Y,Fu Z Y.2004.Study on the early growth and dressed characters in Bantam Chicken[J].Guizhou Agricultural Sciences,32(3):19-21.)
王彬.2015.凤头白鸭与连城白鸭生产性能、遗传结构和部分凤头性状相关基因比较[D].硕士学位论文,扬州大学,导师:陈国宏.pp.39-42(Wang B.2015.Comparision of productivity,hereditary constitution and the expression of certain crest phenotype related genes between crested white duck and Liancheng white ducks[D].Thesis for M.S.,Yangzhou University,Supervisor:Chen G H,pp.39-42.)
赵燕,陈黎,卢立志.2014.家禽缨头性状及其候选基因研究进展[J].畜牧与兽医,46(4):100-102.(Zhao Y,Chen L,Lu L Z.2014.Research progress of poultry crest traits and their candidate genes[J].Animal Husbandry&Veterinary Medicine,46(4):100-102.)
郑艳军.2009.HoxC8基因在小鼠成骨细胞分化中的调控作用研究[D].博士学位论文,吉林大学,导师:朱平,pp.43-83.(Zheng Y Z.2009.Regulation rules of HoxC8 in mouse osteoblast differentiation[D].Thesis for Ph.D.,Ji Lin University,Supervisor:Zhu P,pp.43-83.)
Belting H G,Shashikant C S,Ruddle F H.1998.Modification of expression and cis-regulation of Hoxc8 in the evolution of diverged axial morphology[J].Proceedings of the National Academy of Sciences of the USA,95(5):2355.
Frahm H D,Rehk?mper G.1998.Allometric comparison of the brain and brain structures in the white crested polish chicken with uncrested domestic chicken breeds.[J].Brain,Behavior and Evolution,52(6):292-307.
Romanov M N,Sazanov A A,Smirnov A F.2004.First century of chicken gene study and mapping-a look back and forward[J].Worlds Poultry Science Journal,60(1):19-41.
Shi X,Yang X,Chen D,et al.1999.Smad1 interacts with homeobox DNA-binding proteins in bone morphogenetic protein signaling[J].Journal of Biological Chemistry,274(19):13711-13717.
Huang S,Li Y.2015.HOXC8(homeobox C8)[J].Atlas Genet Cytogenet Oncol Haematol.19(4):266-269.
Joller S,Ammann P,Flury C,et al.2018.Evaluation of HOXC8 in crested Swiss chicken[J].Animal Genetics,49(4):334-336.
Kruger C,Kappen C.2010.Expression of cartilage developmental genes in Hoxc8-and Hoxd4-transgenic mice[J].Plos One,5(2):e8978.
Lei H,Juan A H,Kim M S,et al.2006.Identification of a Hoxc8-regulated transcriptional network in mouse embryo fibroblast cells[J].Proceedings of the National Academy of Sciences of the USA,103(27):10305-10309.
Liu Z.2004.Molecules mimicking Smad1 interacting with hox stimulate bone for mation[J].Journal of Biological Chemistry,279(12):11313-11319.
Li X,Nie S,Chang C,et al.2006.Smads oppose Hox transcriptional activities[J].Experimental Cell Research,312(6):854-864.
Magothe T M,Muhuyi W B,Kahi A K.2010.Influence of major genes for crested-head,frizzle-feather and nakedneck on body weights and growth patterns of indigenous chickens reared intensively in Kenya[J].Tropical Animal Health&Production,42(2):173.
Sun Y,Liu R,Zhao G,et al.2015.Genome-wide linkage analysis identifies loci for physical appearance traits in chickens[J].G3 Genesgenetics,5(10):2037-2041.
Yueh Y G,Kappen G C.1998.Evidence for regulation of cartilage differentiation by the homeobox gene Hoxc-8[J].Proceedings of the National Academy of Sciences of the USA,95(17):9956-9961.
Wang Y,Gao Y,Imsland F,et al.2012.The Crest phenotype in chicken is associated with ectopic expression of,HOXC8,in cranial skin[J].Plos One,7(4):e34012.
Zheng Y J,Chung H J,Min H,et al.2010.In vitro osteoblast differentiation is negatively regulated by Hoxc8[J].Applied Biochemistry&Biotechnology,160(3):891-900.