扶正康复合剂联合顺铂抑制胃癌SGC7901细胞荷瘤裸鼠肿瘤增殖作用的实验研究
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摘要
目的:探讨扶正康复合剂联合顺铂抑制小鼠移植性胃癌的增殖作用。方法:将28只裸鼠随机分为4组:模型对照组,扶正组,顺铂组,顺铂+扶正组,每组7只。皮下注射胃癌SGC7901细胞株,建立胃癌荷瘤裸鼠模型,顺铂及扶正康复合剂连续给药7 d,称取体重及瘤重,计算抑瘤率,并利用PCR法和免疫组化法分别检测瘤体内细胞凋亡靶点(Bcl-2、Bax、Survivin)和血管新生促进因子(VEGF)的mRNA和蛋白的表达情况。结果:在抑瘤率方面,与模型对照组相比,各组裸鼠瘤重均有不同程度的减轻(P<0.05),且顺铂+扶正组抑瘤率最高(66.67%)。在细胞凋亡方面,扶正康复合剂联合顺铂能明显上调Bax的表达,下调Bcl-2、Survivin和VEGF的表达(P<0.05)。结论:扶正康复合剂联合顺铂具有抑制肿瘤增殖的作用。
AIM: To explore the effect of Fuzheng Kangfu mixture combined with cisplatin on promoting apoptosis and inhibiting proliferation of gastric cancer cell. METHODS: Twenty-eight nude mice were randomly divided into 4 groups as follows: the model control group, the group of Fuzheng, the cisplatin group, the group of cisplatin combined with Fuzheng. There were 7 nude mice in each group. The transplanted gastric cancer model of mice was established by subcutaneous injection of gastric cancer SCG7901 cell line. The Cisplatin as well as Fuzheng were administered drugs continuously for 7 days. The weight and tumor weight were measured and the tumor inhibition rate was calculated.The PCR and immunohistochemistry was applied to detect the apoptosis target of Bcl-2, Bax and Survivin as well as VEGF in nude mice. RESULTS:Compared with the model control group, the group of Fuzheng, the cisplatin group, the group of cisplatin combined with Fuzheng, the tumor weights of all the other nude mice were alleviated in different degrees(P<0.05). Among these, the tumor inhibition rate of the group of Cisplatin combined with Fuzheng was the highest(66.67%). In terms of cell inhibition, Fuzheng Kangfu mixture combined with cisplatin could significantly enhance the expression of Bax, and reduce the expressions of Bcl-2, Survivin, and vascular endothelial growth factor(VEGF)(P<0.05).CONCLUSION: Fuzheng Kangfu mixture combined with cisplatin has the effect of inhibiting tumor proliferation.
AIM: To explore the effect of Fuzheng Kangfu mixture combined with cisplatin on promoting apoptosis and inhibiting proliferation of gastric cancer cell. METHODS: Twenty-eight nude mice were randomly divided into 4 groups as follows: the model control group, the group of Fuzheng, the cisplatin group, the group of cisplatin combined with Fuzheng. There were 7 nude mice in each group. The transplanted gastric cancer model of mice was established by subcutaneous injection of gastric cancer SCG7901 cell line. The Cisplatin as well as Fuzheng were administered drugs continuously for 7 days. The weight and tumor weight were measured and the tumor inhibition rate was calculated.The PCR and immunohistochemistry was applied to detect the apoptosis target of Bcl-2, Bax and Survivin as well as VEGF in nude mice. RESULTS:Compared with the model control group, the group of Fuzheng, the cisplatin group, the group of cisplatin combined with Fuzheng, the tumor weights of all the other nude mice were alleviated in different degrees(P<0.05). Among these, the tumor inhibition rate of the group of Cisplatin combined with Fuzheng was the highest(66.67%). In terms of cell inhibition, Fuzheng Kangfu mixture combined with cisplatin could significantly enhance the expression of Bax, and reduce the expressions of Bcl-2, Survivin, and vascular endothelial growth factor(VEGF)(P<0.05).CONCLUSION: Fuzheng Kangfu mixture combined with cisplatin has the effect of inhibiting tumor proliferation.
引文
[1] 段纪俊,严亚琼,杨念念,等.中国恶性肿瘤发病与死亡的国际比较分析[J].中国医学前沿杂志:电子版,2016,8(7):17-23.
[2] 肖鹏,曹雪涛,王青青.恶性肿瘤免疫治疗的现状及展望[J].实用肿瘤杂志,2016,31(1):5-9.
[3] 韩淑萍,余道军,潘莉,等.扶正康复合剂与顺铂联用抑制移植性肺癌的实验研究[J].中华中医药学刊,2017(5):165-170,298-301.
[4] 郭琳琅,曹长安.Bcl-2及相关蛋白bax在人肝胆管癌中的表达及意义[J].临床肝胆病杂志,1998,14(2):85-87.
[5] 唐珍,易芳,郭冬梅,等.细胞凋亡对衰老的调节在肿瘤的发生发展及治疗的研究进展[J].暨南大学学报(自然科学与医学版),2016,37(3):215-222.
[6] 刘友章,于向民.胃癌的中医药临床研究进展[J].新中医,1996(12):52-54.
[7] 陈晓东,潘华峰,蔡甜甜,等.刘友章治疗胃癌前病变临床学术思想探讨[J].中华中医药杂志,2017,32(10):4491-4493.
[8] 冯颖,吴成亚,李杰.“调衡解毒法”辨治胃癌经验[J].中医药导报,2016,22(21):35-37.
[9] 韩淑萍,吴瑾瑾,李晶,等.多指标正交试验法优化扶正康复合剂的提取工艺[J].中华中医药学刊,2014,32(11):2700-2702.
[10] 付大富,梁媛,覃君慧,等.抗凋亡的“原癌基因”Bcl-2实为抗癌基因[J].医学争鸣,2016,7(5):34-37.
[11] 于泽奇,程世翔,涂悦,等.Ki-67、Bcl-2、Bax和Caspase-3在脑胶质瘤组织中的表达及临床意义[J].中国医药导报,2017,14(5):9-12.
[12] 陶旻枫,王小榕.宫颈上皮内瘤变组织中高危型HPV感染对凋亡、增殖基因表达的影响[J].中国医药导报,2017,14(17):80-83,91.
[13] 钟芳芳,吴承龙,孙新芳,等.补阳还五汤联合依达拉奉对脑缺血后细胞凋亡及survivin和caspase-3表达的影响[J].中国临床药理学与治疗学,2016,21(10):1103-1107.
[14] 刘馨莲,綦黄鹏,魏仙.凋亡抑制因子Survivin的研究进展[J].中外医学研究,2017,15(20):161-163.
[15] 曹林忠,汪小敏,张虎林,等.生骨再造丸对兔激素性股骨头坏死VEGF及FLT-1表达的影响[J].中国临床药理学与治疗学,2018,23(8):874-880.
[16] 田君海,米楠楠,孔祥苓,等.低氧条件下人参皂苷Rg3对人喉鳞癌细胞分泌性VEGF的影响[J].疑难病杂志,2018,17(2):180-183.
[17] 王鹤,陈拥军,张中华.白术多糖对胃癌细胞SGC-7901凋亡的影响[J].中国民族民间医药,2015,24(24):10-11.
[18] 郭晨旭,刘静波,赵艳,等白术抑制胃癌细胞SGC-7901增殖并促进其凋亡[J].中国组织化学与细胞化学杂志,2017,26(5):40-46.
[19] 张宇,高飞,王向军,等.铁皮石斛主要化学成分及生物活性研究进展[J].药物生物技术,2015,22(6):557-561.
[20] 金乐红,刘传飞,唐婷,等.石斛多糖抗肿瘤作用的实验研究[J].中国药学杂志,2010,45(22):1734-1737.
[21] 赵益,刘燕,蓝希明,等.铁皮石斛提取物对胃癌癌变的抑制作用及机制研究[J].中草药,2015,46(24):3704-3709.
[2] 肖鹏,曹雪涛,王青青.恶性肿瘤免疫治疗的现状及展望[J].实用肿瘤杂志,2016,31(1):5-9.
[3] 韩淑萍,余道军,潘莉,等.扶正康复合剂与顺铂联用抑制移植性肺癌的实验研究[J].中华中医药学刊,2017(5):165-170,298-301.
[4] 郭琳琅,曹长安.Bcl-2及相关蛋白bax在人肝胆管癌中的表达及意义[J].临床肝胆病杂志,1998,14(2):85-87.
[5] 唐珍,易芳,郭冬梅,等.细胞凋亡对衰老的调节在肿瘤的发生发展及治疗的研究进展[J].暨南大学学报(自然科学与医学版),2016,37(3):215-222.
[6] 刘友章,于向民.胃癌的中医药临床研究进展[J].新中医,1996(12):52-54.
[7] 陈晓东,潘华峰,蔡甜甜,等.刘友章治疗胃癌前病变临床学术思想探讨[J].中华中医药杂志,2017,32(10):4491-4493.
[8] 冯颖,吴成亚,李杰.“调衡解毒法”辨治胃癌经验[J].中医药导报,2016,22(21):35-37.
[9] 韩淑萍,吴瑾瑾,李晶,等.多指标正交试验法优化扶正康复合剂的提取工艺[J].中华中医药学刊,2014,32(11):2700-2702.
[10] 付大富,梁媛,覃君慧,等.抗凋亡的“原癌基因”Bcl-2实为抗癌基因[J].医学争鸣,2016,7(5):34-37.
[11] 于泽奇,程世翔,涂悦,等.Ki-67、Bcl-2、Bax和Caspase-3在脑胶质瘤组织中的表达及临床意义[J].中国医药导报,2017,14(5):9-12.
[12] 陶旻枫,王小榕.宫颈上皮内瘤变组织中高危型HPV感染对凋亡、增殖基因表达的影响[J].中国医药导报,2017,14(17):80-83,91.
[13] 钟芳芳,吴承龙,孙新芳,等.补阳还五汤联合依达拉奉对脑缺血后细胞凋亡及survivin和caspase-3表达的影响[J].中国临床药理学与治疗学,2016,21(10):1103-1107.
[14] 刘馨莲,綦黄鹏,魏仙.凋亡抑制因子Survivin的研究进展[J].中外医学研究,2017,15(20):161-163.
[15] 曹林忠,汪小敏,张虎林,等.生骨再造丸对兔激素性股骨头坏死VEGF及FLT-1表达的影响[J].中国临床药理学与治疗学,2018,23(8):874-880.
[16] 田君海,米楠楠,孔祥苓,等.低氧条件下人参皂苷Rg3对人喉鳞癌细胞分泌性VEGF的影响[J].疑难病杂志,2018,17(2):180-183.
[17] 王鹤,陈拥军,张中华.白术多糖对胃癌细胞SGC-7901凋亡的影响[J].中国民族民间医药,2015,24(24):10-11.
[18] 郭晨旭,刘静波,赵艳,等白术抑制胃癌细胞SGC-7901增殖并促进其凋亡[J].中国组织化学与细胞化学杂志,2017,26(5):40-46.
[19] 张宇,高飞,王向军,等.铁皮石斛主要化学成分及生物活性研究进展[J].药物生物技术,2015,22(6):557-561.
[20] 金乐红,刘传飞,唐婷,等.石斛多糖抗肿瘤作用的实验研究[J].中国药学杂志,2010,45(22):1734-1737.
[21] 赵益,刘燕,蓝希明,等.铁皮石斛提取物对胃癌癌变的抑制作用及机制研究[J].中草药,2015,46(24):3704-3709.