响应面法优化猕猴桃叶绿素提取工艺
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摘要
以宜昌绿肉猕猴桃果肉为实验材料,采用浸提法,在单因素实验基础上,以OD_(666)值为指标,根据Box-Behnken设计,采用4因素3水平响应面分析方法,对叶绿素提取条件进行优化研究,并对各因素(提取剂浓度、液料比、提取时间和提取温度)的显著性和交互作用进行了分析。结果表明:提取时间和提取温度是影响叶绿素提取的极显著因素(p<0.01)。优化所得工艺参数为:提取溶剂乙酸乙酯、液料比1.22∶1 mL/g、乙酸乙酯体积分数59.6%、提取时间236 min、提取温度59℃、吸光值为0.636±0.01,此时通过Arnon法测得叶绿素含量为27.73μg/g。
Taking Yichang green kiwifruit pulp as experimental material,using organic solvent extraction,basing on single factor experiments with OD_(666) as an indicator,according to the Box-Behnken design,a 4-factor 3-level response surface analysis method was used to optimize chlorophyll extraction conditions,and the significance and interaction of various factors(concentration of extraction solvent,liquid to material ratio,extraction time,extraction temperature)were analyzed. Results showed that extraction time and extraction temperature were extremly significant factors affecting chlorophyll extraction(p<0.01).The optimized process parameters were as follows:extraction solvent was ethyl acetate,liquid/solid ratio was 1.22∶1 mL/g,ethyl acetate volume fraction was 59.6%,extraction time was 236 min,extraction temperature was 59 ℃,absorbance value was 0.636±0.01. At this time,the chlorophyll content was 27.73 μg/g as measured by the Arnon method.
Taking Yichang green kiwifruit pulp as experimental material,using organic solvent extraction,basing on single factor experiments with OD_(666) as an indicator,according to the Box-Behnken design,a 4-factor 3-level response surface analysis method was used to optimize chlorophyll extraction conditions,and the significance and interaction of various factors(concentration of extraction solvent,liquid to material ratio,extraction time,extraction temperature)were analyzed. Results showed that extraction time and extraction temperature were extremly significant factors affecting chlorophyll extraction(p<0.01).The optimized process parameters were as follows:extraction solvent was ethyl acetate,liquid/solid ratio was 1.22∶1 mL/g,ethyl acetate volume fraction was 59.6%,extraction time was 236 min,extraction temperature was 59 ℃,absorbance value was 0.636±0.01. At this time,the chlorophyll content was 27.73 μg/g as measured by the Arnon method.
引文
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[22]海洪,汪坤,金文英,等. Box-Behnken响应面法优化超声波提取蚕沙中叶绿素的工艺研究[J].食品工业科技,2009,30(3):207-209,211.
[23]刘彩云.提取方法、试剂对不同高等植物叶片叶绿素提取效果的比较分析[J].潍坊学院学报,2014,14(2):74-76.
[24]张辉,张娜娜,马丽,等.响应面法优化大麦苗叶绿素提取工艺[J].食品科学,2014,35(2):75-80.
[2]孙兢喆.猕猴桃属植物的现代药理学研究进展[J].当代医药论丛,2014,12(19):136-137.
[3]张婷,李琛,罗安伟,等. 8种猕猴桃不同组织部位的体外抗氧化活性[J].食品科学,2016,37(19):88-93.
[4]郑国栋,欧阳文,颜苗,等.叶绿素及其衍生物的药理研究进展[J].中南药学,2006,4(2):146-148.
[5]Serpeloni J M,Batista B L,Angeli J P,et al.Antigenotoxic properties of chlorophyll b against cisplatin-induced DNA damage and its relationship with distribution of platinum and magnesium in vivo[J]. Journal of Toxicology and Environmental Health,2013,76:345-353.
[6]张丽华,李顺峰,刘兴华,等.猕猴桃果肉叶绿素的提取及抗氧化活性研究[J].中国食品学报,2012,12(7):29-35.
[7]邓祥元,成婕,刘孟姣,等.响应面法优化小球藻叶绿素提取工艺及其稳定性研究[J].东北农业大学学报,2015,46(7):40-49.
[8]敖国富,周甜敏,张来.冬青叶鼠刺叶绿素的提取及其稳定性[J].贵州农业科学,2015,43(10):82-85.
[9]张丽华.猕猴桃果实制浆中叶绿素降解机理及其护绿方法研究[D].咸阳:西北农林科技大学,2012.
[10]李淤慧,于开源,鞠晓峰,等.苦瓜叶绿素提取方法优化[J].农产品加工,2015(8):28-30.
[11]邓祥元,沙鹏,高坤.微波辅助萃取蚕沙叶绿素的工艺研究[J].东北农业大学学报,2012,43(8):12-16.
[12]M D Macías-Sánchez,Mantell C,Rodrí Guez M,et al. Supercritical fluid extraction of carotenoids and chlorophyll a from Nannochloropsis gaditana[J]. Journal of Food Engineering,2005,66(2):245-251.
[13]刘辉,贺菊萍,秦杰,等.海带中叶绿素的提取及间接测定方法研究[J].食品科学,2010,31(18):178-181.
[14]张秀君,孙钱钱,乔双,等.菠菜叶绿素提取方法的比较研究[J].作物杂志,2011(3):57-60.
[15]葛俊珂,孙丽娜,赵涵,等.叶绿素的提取方法及药理作用进展[J].科技创新与应用,2016(24):14.
[16]Esteban R,Garcíaplazaola J I,Hernández A,et al. On the recalcitrant use of Arnon’s method for chlorophyll determination.[J]. New Phytologist,2017,217(2):474-476.
[17]任亚梅.猕猴桃果实叶绿素代谢及生理特性研究[D].杨凌:西北农林科技大学,2009.
[18]任丹丹,任先见,王惜童,等.萱藻中主要类胡萝卜素的分析与鉴定[J].食品工业科技,2017,38(2):59-62.
[19]黄持都,胡小松,廖小军,等.叶绿素研究进展[J].中国食品添加剂,2007(3):114-118.
[20]刘静,张爱民,李湘利,等.红香椿叶绿素的提取工艺与稳定性的研究[J].中国酿造,2012,31(3):82-86.
[21]张志忠,朱书芳,宋鹏,等.从三叶草中提取叶绿素的工艺研究[J].中国食品添加剂,2012(5):115-118.
[22]海洪,汪坤,金文英,等. Box-Behnken响应面法优化超声波提取蚕沙中叶绿素的工艺研究[J].食品工业科技,2009,30(3):207-209,211.
[23]刘彩云.提取方法、试剂对不同高等植物叶片叶绿素提取效果的比较分析[J].潍坊学院学报,2014,14(2):74-76.
[24]张辉,张娜娜,马丽,等.响应面法优化大麦苗叶绿素提取工艺[J].食品科学,2014,35(2):75-80.