川秋葵微粉降血糖活性研究
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摘要
热风干燥制备川秋葵微粉,研究川秋葵微粉在体内和体外的降血糖活性。结果表明:川秋葵微粉具有良好的体外降血糖活性,阿卡波糖对α-葡萄糖苷酶抑制活性优于秋葵微粉,但差异不显著(P> 0.05);阿卡波糖对α-淀粉酶活性抑制活性优于秋葵微粉,差异不显著(P> 0.05);当葡萄糖溶液浓度为50 mmol/L时,秋葵微粉对葡萄糖吸附能力最强(P <0.01),为(397.08±0.64)mg/g;秋葵微粉对葡萄糖透析延迟指数随透析时间延长先增加后降低(P <0.05),60 min达到最高值(49.63±4.45)%。根据国际认可的Wolever和Salmeron公式分别计算得到秋葵微粉的血糖生成指数(glycemic index,GI)为40.8,血糖负荷(glycemic load,GL)为9.1,均为低GI低GL食品;相较于葡萄糖粉血糖应答曲线的速升速降,秋葵微粉的血糖应答曲线均呈缓升缓降趋势,曲线波动小,有利于机体餐后血糖的稳定。
In order to study the hypoglycemic activity of Sichuan okra micropowder in vitro and in vivo,the dry powder of okra was prepared by hot air drying. The results showed that the micropowder of okra had good in vitro hypoglycemic activity,and the inhibitory activity of acarbose on α-glucosidase was better than that of okra micropowder,but the difference was not significant(P>0.05). The inhibitory activity of acarbose on α-amylase was better than that of okra powder,but the difference was not significant(P>0.05). When the concentration of glucose solution was 50 mmol/L,the glucose adsorption ability of okra powder was the strongest(P <0.01),which was(397.08±0.64)mg/g. The glucose dialysis delay index of okra powder increased with dialysis duration(P<0.05),and the highest value of 60 min reached(49.63±4.45)%. According to the internationally recognizedWolever and Salmeron formulas,the glycemic index(GI)value of the okra powder was calculated 40.8,and the glycemic load(GL)value was 9.1. All of them were low GI and low GL food. Compared with the rapid rise and speed drop of glucose response curve of glucose powder,the blood glucose response curve of the micropowder of okra was slowly rising and decreasing,and the curve fluctuated slightly,which was beneficial to the stability of postprandial blood glucose.
In order to study the hypoglycemic activity of Sichuan okra micropowder in vitro and in vivo,the dry powder of okra was prepared by hot air drying. The results showed that the micropowder of okra had good in vitro hypoglycemic activity,and the inhibitory activity of acarbose on α-glucosidase was better than that of okra micropowder,but the difference was not significant(P>0.05). The inhibitory activity of acarbose on α-amylase was better than that of okra powder,but the difference was not significant(P>0.05). When the concentration of glucose solution was 50 mmol/L,the glucose adsorption ability of okra powder was the strongest(P <0.01),which was(397.08±0.64)mg/g. The glucose dialysis delay index of okra powder increased with dialysis duration(P<0.05),and the highest value of 60 min reached(49.63±4.45)%. According to the internationally recognizedWolever and Salmeron formulas,the glycemic index(GI)value of the okra powder was calculated 40.8,and the glycemic load(GL)value was 9.1. All of them were low GI and low GL food. Compared with the rapid rise and speed drop of glucose response curve of glucose powder,the blood glucose response curve of the micropowder of okra was slowly rising and decreasing,and the curve fluctuated slightly,which was beneficial to the stability of postprandial blood glucose.
引文
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[34]Chau C F,Chen C H,Lin C Y.Insoluble fiber-rich fraction derived from Averrhoa caramola:Hypoglycemic effects determined by in vitro methods[J].LWT-Food Science and Technology,2004,37(3):331-335
[35]Adiotom Re J,Eastwood M A,Edwa Rdsa,et al.Dietary fibre:In vitro methods that anticipate nutrition and metabolic activity in humans[J].American Journal of Clinical Nutrition,1990,52(1):128-134
[36]冯雁波,包怡红.超微粉碎对松仁膳食纤维体外降血糖、降血脂功能的影响[J].食品工业科技,2016,37(23):342-346,350
[37]Chau C F,Chen C H,Lin C Y.Insoluble fiber-rich fractions derived from Averrhoa carambola:hypoglycemic effects determined by in vitro methods[J].LWT-Food Science and Technology,2004,37(3):331-335.
[38]杨月欣,崔红梅,王岩,等.常见谷类和薯类的血糖生成指数[J].营养学报,2003,25(2):185-189
[39]吴铁良.代谢综合征诊治进展[J].现代预防医学,2010,37(16):3200-3202
[2]郭君,银金兰,康桂英.具有降血糖活性的植物药[J].内蒙古医学杂志,2004(5):362-363
[3]张丽娜.水翁花对胰脂肪酶和α-淀粉酶抑制活性及作用机制初步探讨[D].上海:华东理工大学,2012
[4]刘娜.黄秋葵的综合利用及前景[J].中国食物与营养,2006(6):27-30.DOI:10.5297/ser.1201.002
[5]V Sabitha,S Ramachandran,K R Naveen,et al.Antidiabetic and antihyperlipidemic potential of Abelmoschus esculentus(L.)Moench.in streptozotocin-induced diabetic rats[J].Journal of pharmacy&bioallied sciences,2011,3(3):397-402
[6]Okada Y,Okada M,Sagesaka Y.Screening of Dried Plant Seed Extracts for Adiponectin Production Activity and Tumor Necrosis Factor-Alpha Inhibitory Activity on 3T3-L1 Adipocytes[J].Plant Foods for Human Nutrition,2010,65(3):225-232
[7]Yamada K,Anai T,Hasegawa K.Lepidimoide,an allelopathic substance in the exudates from germinated seeds[J].Phytochemistry,1995,39(5):1031-1032
[8]张娜.黄秋葵果胶的提取工艺研究[J].安徽农业科学,2012,40(35):17319-17321
[9]刘怡彤,段振华,马华林,等.超声波辅助提取黄秋葵多酚和多糖的工艺研究[J].食品工业科技,2013,34(21):246-253
[10]方晴霞,金戈.黄秋葵中总黄酮的含量测定[J].医学导报,2004,23(9):675
[11]刘雪蕊.黄秋葵多糖的提取及其降血糖活性研究[D].天津:天津科技大学,2017
[12]冯冠英,宋晓雪,徐志立,等.黄秋葵对糖尿病小鼠的降血糖作用[J].转化医学电子杂志,2017,4(11):44-47
[13]闫天龙.黄秋葵系列产品开发及关键技术研究[D].成都:西华大学,2016
[14]李加兴,石春诚,马浪,等.黄秋葵果胶理化特性的研究[J].食品科学,2015,36(17):104-108
[15]李孟秋,翟俊乐,田欢,等.黄秋葵提取物体外抗氧化活性的研究[J].中国食品添加剂,2015(10):65-69
[16]Chau C F,Huang Y L.Comparison of the Chemical Composition and Physicochemical Properties of Different Fibers Prepared from the Peel of Citrus sinensis L.Cv.Liucheng[J].Journal of Agricultural and Food Chemistry,2003,51(9):2615-2618
[17]梅新,木泰华,陈学玲,等.超微粉碎对甘薯膳食纤维成分及物化特性影响[J].中国粮油学报,2014,29(2):76-81
[18]Daou C,Zhang H.Study on Functional Properties of Physically Modified Dietary Fibres Derived from Defatted Rice Bran[J].Journal of Agricultural Science,2012,4(9):85-97
[19]黄清霞,雷激,李华鑫,等.高生物活性柠檬膳食纤维的功能特性研究[J].食品工业科技,2012,33(5):226-229
[20]黄冬云,钱海峰,苑华宁,等.木聚糖酶制取米糠膳食纤维的功能性质[J].食品与发酵工业,2013,39(12):30-34
[21]郭晓蕾,朱思潮,翟旭峰,等.硫酸蒽酮法与硫酸苯酚法测定灵芝多糖含量比较[J].中华中医药学刊,2010,28(9):2000-2002
[22]李小玲,张文青,卫姝岑,等.燕麦荞麦加工食品血糖生成指数与血糖负荷的测定[J].临床医药实践,2017,26(4):249-252
[23]WOLEVER T M S,DAVID J A,ALEXANDR A L J.The glycemic index:methodology and clinical implication[J].Am J Clin Nutr,1991(54):864-865
[24]SALMERN J,ASCHERIO A,RIMM E B,et al.Dietary fiber,glycemic load,and risk of NIDDM in men[J].Diabetes Care,1997,20(4):545-550
[25]APOSTOLIDIS E,KWON Y I,SHETTY K.Potential of cranberrybased herbal synergies for diabetes and hypertension management[J].Asia Pacific Journal of Clinical Nutrition,2006,15(3):433-441
[26]刘睿,潘思轶,刘亮,等.高粱原花青素对α-淀粉酶活力抑制动力学的研究[J].食品科学,2005,26(9):189-192
[27]陈菊红,顾正彪,洪雁.改性马铃薯渣的功能特性研究[J].食品工业科技,2009,30(1):130-133
[28]郭雅靖,颜梦婷,林圣楠,等.几种原花青素的降血糖作用及与常见食品原料的结合研究[J].食品科学,2017,38(19):156-163
[29]杨远通.超微粉碎对猕猴桃渣膳食纤维功能性质的影响[J].食品与机械,2011,27(1):11-14
[30]常世敏,张玉星,田益玲.超临界CO2对梨渣不溶性膳食纤维物化特性的影响[J].食品研究与开发,2016,37(7):97-101
[31]LOPEZ G,ZOS G,RINCON F,et al.Relationship between physical and hydration properties of soluble and insoluble fibre of artichoke[J].Journal of Agricultural and Food Chemistry,1996,44(9):2773-2778
[32]ROD RIGUEZ-GUTIERREZ G,RUIBO-SENENT F,LAMA-MUNOZ A,et al.Properties of lignin,cellulose,and hemicelluloses isolated from olive cake and olive stones:Binding of water,oil,bile acids,and glucose[J].Journal of Agricultural and Food Chemistry,2014,62(36):8973-8981
[33]CAMIRE M E,DOUGHERTY M P.Raisin dietary fibrecomposition and in vitro bile acid binding[J].Journal of Agricultural and Food Chemistry,2003,51(3):834-837
[34]Chau C F,Chen C H,Lin C Y.Insoluble fiber-rich fraction derived from Averrhoa caramola:Hypoglycemic effects determined by in vitro methods[J].LWT-Food Science and Technology,2004,37(3):331-335
[35]Adiotom Re J,Eastwood M A,Edwa Rdsa,et al.Dietary fibre:In vitro methods that anticipate nutrition and metabolic activity in humans[J].American Journal of Clinical Nutrition,1990,52(1):128-134
[36]冯雁波,包怡红.超微粉碎对松仁膳食纤维体外降血糖、降血脂功能的影响[J].食品工业科技,2016,37(23):342-346,350
[37]Chau C F,Chen C H,Lin C Y.Insoluble fiber-rich fractions derived from Averrhoa carambola:hypoglycemic effects determined by in vitro methods[J].LWT-Food Science and Technology,2004,37(3):331-335.
[38]杨月欣,崔红梅,王岩,等.常见谷类和薯类的血糖生成指数[J].营养学报,2003,25(2):185-189
[39]吴铁良.代谢综合征诊治进展[J].现代预防医学,2010,37(16):3200-3202