油茶皂苷对小鼠DSS诱导结肠炎影响的研究（英文）
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摘要
目的:探讨SQS是否能预防葡聚糖硫酸钠(DSS)诱导的实验性结肠炎。方法:将C57BL/6小鼠随机分为5组。实验组用不同浓度的SQS灌胃21天,然后用DSS处理5天,诱导结肠炎并记录小鼠体重。测量结肠长度,收集结肠并进行苏木精-伊红(H&E)染色。测定炎症因子(TNF-α、IL-1β、MCP-1和i NOS)在结肠中的基因表达。结果:与对照组相比,模型组体重显著下降和结肠长度显著缩短。但与DSS模型组比较,SQS组体重下降、结肠长度、HE染色及炎性细胞因子基因表达等指标均不能观察到炎症的缓解。结论:在目前的研究中,SQS在我们的研究中未显示出预防DSS诱导的结肠炎的效果。
Objective: Sasanquasaponin( SQS) is an extract from Camellia oleifera Abel,which is considered to have an anti-inflammatory property. This study is to determine whether SQS can prevent experiment colitis induced by dextran sodium sulfate( DSS). Method: C57BL/6 mice were randomly divided into 5 groups. The experiment groups were treated by intragastric injection with different concentrates of SQS for 21 days,and then treated with DSS for 5 days to induce colitis. We recorded the body weights and colon lengths of mice. colons were also sectioned for hematoxylin &eosin( H&E) staining. The gene expression of pro-inflammatory cytokines,including TNF-a,IL-1β,MCP-1,and i NOS in colon were determined. Results: Compared to the control group,body weights and colon lengths in the DSS group were decreased significantly. But,we could not observe remission of inflammation based on the parameters such as body weight,colon length and H&E staining,and gene expression of pro-inflammatory cytokines,in SQS-treated groups in comparison with DSS model group. Conclusions: SQS had not shown the effect of preventing DSS-induced colitis in our current study.
Objective: Sasanquasaponin( SQS) is an extract from Camellia oleifera Abel,which is considered to have an anti-inflammatory property. This study is to determine whether SQS can prevent experiment colitis induced by dextran sodium sulfate( DSS). Method: C57BL/6 mice were randomly divided into 5 groups. The experiment groups were treated by intragastric injection with different concentrates of SQS for 21 days,and then treated with DSS for 5 days to induce colitis. We recorded the body weights and colon lengths of mice. colons were also sectioned for hematoxylin &eosin( H&E) staining. The gene expression of pro-inflammatory cytokines,including TNF-a,IL-1β,MCP-1,and i NOS in colon were determined. Results: Compared to the control group,body weights and colon lengths in the DSS group were decreased significantly. But,we could not observe remission of inflammation based on the parameters such as body weight,colon length and H&E staining,and gene expression of pro-inflammatory cytokines,in SQS-treated groups in comparison with DSS model group. Conclusions: SQS had not shown the effect of preventing DSS-induced colitis in our current study.
引文
[1]Xavier RJ,Podolsky DK.Unravelling the pathogenesis of inflammatory bowel disease[J].Nature,2007,448(7152):427-34.
[2]Loftus EV.Clinical epidemiology of inflammatory bowel disease:incidence,prevalence,and environmental influences[J].Gastroenterology,2004,126(6):1504-17.
[3]Xue Y,Ouyang Q,Huang Z,et al.Advances in IBD research in China in the last two decades-20-year literature summary[J].International Journal of Digestive Diseases,2013,33(4):278.
[4]Triantafillidis JK,Nasioulas G,Kosmidis PA.Colorectal cancer and inflammatory bowel disease:epidemiology,risk factors,mechanisms of carcinogenesis and prevention strategies[J].Anticancer Research,2009,29(7):2727-37.
[5]Liu Z,Ramer-Tait AE,Henderson AL,et al.Helicobacter bilis colonization enhances susceptibility to Typhlocolitis following an inflammatory trigger[J].Dig Dis Sci,2011,56(10):2838-48.
[6]Man SM,Zhu Q,Zhu L,et al.Critical Role for the DNASensor AIM2 in Stem Cell Proliferation and Cancer[J].Cell,2015,162(1):45-58.
[7]Hendrickson BA,Gokhale R,Cho JH.Clinical Aspects and Pathophysiology of Inflammatory Bowel Disease[J].Clinical Microbiology Reviews,2002,15(1):79-94.
[8]Domènech E.Inflammatory Bowel Disease:Current Therapeutic Options[J].Digestion,2006,73(1):67-76.
[9]Li J,Wang D.Research Progress on medicinal value of Camellia oleifera[J].Journal of Yichun College,2011,33(4):128.
[10]Lee CP,Shih PH,Hsu CL,et al.Hepatoprotection of tea seed oil(Camellia oleifera Abel.)against CCl4-induced oxidative damage in rats[J].Food and Chemical Toxicology,2007,45(6):888-95.
[11]Cheng YT,Wu SL,Ho CY,et al.Beneficial effects of Camellia Oil(Camellia oleifera Abel.)on ketoprofen-induced gastrointestinal mucosal damage through upregulation of HO-1 and VEGF[J].Journal of Agricultural and Food Chemistry,2014,62(3):642-50.
[12]Tu PS,Tung YT,Lee WT,et al.Protective Effect of Camellia Oil(Camellia oleifera Abel.)against EthanolInduced Acute Oxidative Injury of the Gastric Mucosa in Mice[J].Journal of Agricultural and Food Chemistry,2017,65(24):4932-41.
[13]Lee WT,Tung YT,Wu CC,et al.Camellia Oil(Camellia oleifera Abel.)Modifies the Composition of Gut Microbiota and Alleviates Acetic Acid-Induced Colitis in Rats[J].Journal of Agricultural and Food Chemistry,2018,66(28):7384-92.
[14]Xiao X,He L,Chen Y,et al.Anti-inflammatory and antioxidative effects of Camellia oleifera Abel components[J].Future Medicinal Chemistry,2017,9(17):2069-79.
[15]Zhang XF,Yang SL,Han YY,et al.Qualitative and quantitative analysis of triterpene saponins from tea seed pomace(Camellia oleifera Abel)and their activities against bacteria and fungi[J].Molecules,2014,19(6):7568-80.
[16]Hu J-L,Nie S-P,Huang D-F,et al.Antimicrobial activity of saponin-rich fraction from Camellia oleifera cake and its effect on cell viability of mouse macrophage RAW264.7[J].Journal of the Science of Food and Agriculture,2012,92(12):2443-9.
[17]Zong J,Wang R,Bao G,et al.Novel triterpenoid saponins from residual seed cake of Camellia oleifera Abel.show anti-proliferative activity against tumor cells[J].Fitoterapia,2015,104:7-13.
[18]Zeng J,Chen S,Li N,et al.Sasanquasaponin from Camellia oleifera Abel.induces apoptosis via Bcl-2,Bax and caspase-3 activation in Hep G2 cells[J].Molecular Medicine Reports,2015,12(2):1997-2002.
[19]Chen L,Chen J,Xu H.Sasanquasaponin from Camellia oleifera Abel.induces cell cycle arrest and apoptosis in human breast cancer MCF-7 cells[J].Fitoterapia,2013,84:123-129.
[20]Huang Q,Shao L,He M,et al.Inhibitory effects of sasanquasaponin on over-expression of ICAM-1 and on enhancement of capillary permeability induced by burns in rats[J].Burns,2005,31(5):637-42.
[21]Ye Y,Xing H,Chen X.Anti-inflammatory and analgesic activities of the hydrolyzed sasanquasaponins from the defatted seeds of Camellia oleifera[J].Archives of Pharmacal Research,2013,36(8):941-51.
[2]Loftus EV.Clinical epidemiology of inflammatory bowel disease:incidence,prevalence,and environmental influences[J].Gastroenterology,2004,126(6):1504-17.
[3]Xue Y,Ouyang Q,Huang Z,et al.Advances in IBD research in China in the last two decades-20-year literature summary[J].International Journal of Digestive Diseases,2013,33(4):278.
[4]Triantafillidis JK,Nasioulas G,Kosmidis PA.Colorectal cancer and inflammatory bowel disease:epidemiology,risk factors,mechanisms of carcinogenesis and prevention strategies[J].Anticancer Research,2009,29(7):2727-37.
[5]Liu Z,Ramer-Tait AE,Henderson AL,et al.Helicobacter bilis colonization enhances susceptibility to Typhlocolitis following an inflammatory trigger[J].Dig Dis Sci,2011,56(10):2838-48.
[6]Man SM,Zhu Q,Zhu L,et al.Critical Role for the DNASensor AIM2 in Stem Cell Proliferation and Cancer[J].Cell,2015,162(1):45-58.
[7]Hendrickson BA,Gokhale R,Cho JH.Clinical Aspects and Pathophysiology of Inflammatory Bowel Disease[J].Clinical Microbiology Reviews,2002,15(1):79-94.
[8]Domènech E.Inflammatory Bowel Disease:Current Therapeutic Options[J].Digestion,2006,73(1):67-76.
[9]Li J,Wang D.Research Progress on medicinal value of Camellia oleifera[J].Journal of Yichun College,2011,33(4):128.
[10]Lee CP,Shih PH,Hsu CL,et al.Hepatoprotection of tea seed oil(Camellia oleifera Abel.)against CCl4-induced oxidative damage in rats[J].Food and Chemical Toxicology,2007,45(6):888-95.
[11]Cheng YT,Wu SL,Ho CY,et al.Beneficial effects of Camellia Oil(Camellia oleifera Abel.)on ketoprofen-induced gastrointestinal mucosal damage through upregulation of HO-1 and VEGF[J].Journal of Agricultural and Food Chemistry,2014,62(3):642-50.
[12]Tu PS,Tung YT,Lee WT,et al.Protective Effect of Camellia Oil(Camellia oleifera Abel.)against EthanolInduced Acute Oxidative Injury of the Gastric Mucosa in Mice[J].Journal of Agricultural and Food Chemistry,2017,65(24):4932-41.
[13]Lee WT,Tung YT,Wu CC,et al.Camellia Oil(Camellia oleifera Abel.)Modifies the Composition of Gut Microbiota and Alleviates Acetic Acid-Induced Colitis in Rats[J].Journal of Agricultural and Food Chemistry,2018,66(28):7384-92.
[14]Xiao X,He L,Chen Y,et al.Anti-inflammatory and antioxidative effects of Camellia oleifera Abel components[J].Future Medicinal Chemistry,2017,9(17):2069-79.
[15]Zhang XF,Yang SL,Han YY,et al.Qualitative and quantitative analysis of triterpene saponins from tea seed pomace(Camellia oleifera Abel)and their activities against bacteria and fungi[J].Molecules,2014,19(6):7568-80.
[16]Hu J-L,Nie S-P,Huang D-F,et al.Antimicrobial activity of saponin-rich fraction from Camellia oleifera cake and its effect on cell viability of mouse macrophage RAW264.7[J].Journal of the Science of Food and Agriculture,2012,92(12):2443-9.
[17]Zong J,Wang R,Bao G,et al.Novel triterpenoid saponins from residual seed cake of Camellia oleifera Abel.show anti-proliferative activity against tumor cells[J].Fitoterapia,2015,104:7-13.
[18]Zeng J,Chen S,Li N,et al.Sasanquasaponin from Camellia oleifera Abel.induces apoptosis via Bcl-2,Bax and caspase-3 activation in Hep G2 cells[J].Molecular Medicine Reports,2015,12(2):1997-2002.
[19]Chen L,Chen J,Xu H.Sasanquasaponin from Camellia oleifera Abel.induces cell cycle arrest and apoptosis in human breast cancer MCF-7 cells[J].Fitoterapia,2013,84:123-129.
[20]Huang Q,Shao L,He M,et al.Inhibitory effects of sasanquasaponin on over-expression of ICAM-1 and on enhancement of capillary permeability induced by burns in rats[J].Burns,2005,31(5):637-42.
[21]Ye Y,Xing H,Chen X.Anti-inflammatory and analgesic activities of the hydrolyzed sasanquasaponins from the defatted seeds of Camellia oleifera[J].Archives of Pharmacal Research,2013,36(8):941-51.