~(60)Co-γ辐射对谷稗幼苗生长及生理效应的影响
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摘要
【目的】探究不同~(60)Co-γ射线辐射对朝牧1号谷稗种子幼苗生长以及生理效应的影响。【方法】以朝牧1号谷稗种子为试材,采用0、50、100、150、200和250 Gy的~(60)Co-γ射线辐射谷稗种子,对不同辐射剂量的谷稗种子进行幼苗培养,测定辐射对其幼苗的生长和叶片生理生化的影响,并对各指标进行相关性和主成分分析。【结果】辐射抑制了谷稗幼苗的生长,250 Gy对其幼苗的生长抑制作用较强;随着辐射剂量的增加,可溶性糖、脯氨酸、可溶性蛋白和总叶绿素含量均呈先增后减的变化趋势,相对电导率和丙二醛含量呈先减后增的变化趋势,过氧化物酶(POD)、过氧化氢酶(CAT)和超氧化物歧化酶(SOD)活性均呈先增后减的变化趋势。【结论】综合主成分的方差贡献率和主成分的原性状相关矩阵值计算出特征向量分析发现,50～200 Gy辐射剂量可用于谷稗的辐射育种。
[Purpose]To investigate the effects of different ~(60)Co-γ-rays irradiation for Echinochloa crusgalli ‘Zhaomu No.1' on the growth and physiological effects of seedlings.[Method]The seeds of E. crusgalli ‘Zhaomu No.1' were used as the test material and treated with different doses of ~(60)Co-γradiation. ~(60)Co-γ-ray irradiation dose was 0, 50, 100, 150, 200 and 250 Gy. E. crusgalli seeds were cultured seedlings with different radiation doses. The changes of growth, physiological and biochemical indexes of leaves were measured and the indexes were analyzed by correlation and principal component analysis.[Result]Radiation inhibited the growth of E. crusgalli seedlings, and 250 Gy doses of radiation had a stronger inhibitory effect on their seedlings. With the increase of radiation dose, soluble sugar, proline, soluble protein and total chlorophyll content was increased first and then decreased trend. The relative conductivity and malondialdehyde content decreased first and then increased. Peroxidase(POD) and catalase(CAT) and superoxide dismutase(SOD) showed a trend of increased first and then decreased.[Conclusion]By analyzing the variance contribution ratio of principal components and the correlation matrix value of principal components, the characteristic vector analysis shows that the 50-200 Gy radiation dose can be used in the radiation breeding of E. crusgalli.
[Purpose]To investigate the effects of different ~(60)Co-γ-rays irradiation for Echinochloa crusgalli ‘Zhaomu No.1' on the growth and physiological effects of seedlings.[Method]The seeds of E. crusgalli ‘Zhaomu No.1' were used as the test material and treated with different doses of ~(60)Co-γradiation. ~(60)Co-γ-ray irradiation dose was 0, 50, 100, 150, 200 and 250 Gy. E. crusgalli seeds were cultured seedlings with different radiation doses. The changes of growth, physiological and biochemical indexes of leaves were measured and the indexes were analyzed by correlation and principal component analysis.[Result]Radiation inhibited the growth of E. crusgalli seedlings, and 250 Gy doses of radiation had a stronger inhibitory effect on their seedlings. With the increase of radiation dose, soluble sugar, proline, soluble protein and total chlorophyll content was increased first and then decreased trend. The relative conductivity and malondialdehyde content decreased first and then increased. Peroxidase(POD) and catalase(CAT) and superoxide dismutase(SOD) showed a trend of increased first and then decreased.[Conclusion]By analyzing the variance contribution ratio of principal components and the correlation matrix value of principal components, the characteristic vector analysis shows that the 50-200 Gy radiation dose can be used in the radiation breeding of E. crusgalli.
引文
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[16]熊秋芳,陈玉霞,张雪清,等.60Co-γ辐射对萝卜种子萌发和幼苗生长的影响[J].浙江农业科学,2014,1(3):356.DOI:10.16178/j.issn.0528-9017.2014.03.033.
[17]张玉,白史且,李达旭,等.60Co-γ辐射对菊苣种子发芽及幼苗生理的影响[J].草地学报,2013,21(1):147.
[18]陈斌,张传玲,江红生,等.纳米银诱导拟南芥活性氧自由基的积累和抗氧化系统的改变[J].基因组学与应用生物学,2017(4):1646.DOI:10.13417/j.gab.036.001646.
[19]廖飞雄,潘瑞炽.60Co-γ辐射菜心种子对苗期细胞膜及保护酶活性的影响[J].核农学报,2001,15(5):286.
[20]王月华,韩烈保,尹淑霞,等.60Co-γ射线辐射对早熟禾种子发芽及种子内酶活性的影响[J].中国草地学报,2006,28(1):54.
[2]杨震,彭选明,彭伟正.作物诱变育种研究进展[J].激光生物学报,2016,25(4):302.DOI:10.3969/j.issn.1007-7146.
[3]徐振东,兰秀红,杨曼,等.06Co-γ辐射对2种高羊茅染色体核型的影响[J].东北农业科学,2016(3):18.DOI:10.16423/j.cnki.1003-8701.2016.03.005.
[4]谢新春,刘天增,解新明,等.60Co-γ射线对海滨雀稗成活率及生长特性的影响[J].华南农业大学学报,2015,36(4):65.DOI:10.7671/j.issn.1001-411X.2015.04.012.
[5]姚银安,杨爱华,徐刚.低剂量60Co-γ辐射种子预处理对黑麦草紫外线B辐射伤害的保护作用[J].种子,2008,27(12):25.DOI:10.16590/j.cnki.1001-4705.2008.12.050.
[6]李红,李波,杨曌,等.60Co-γ射线辐射对苜蓿幼苗生长和两种抗氧化酶活性的影响[J].草地学报,2018(1):216.DOI:10.11733/j.issn.1007-0435.2018.01.027.
[7]王谧,王芳,王舰.应用隶属函数法对马铃薯进行抗旱性综合评价[J].云南农业大学学报(自然科学),2014,29(4):476.DOI:10.3969/j.issn.1004-390X(n).2014.04.002.
[8]毕润霞,郝兆祥,侯乐峰,等.电导法评价石榴抗寒性方法的探讨[J].山东农业科学,2015(2):38.DOI:10.14083/j.issn.1001-4942.2015.02.009.
[9]尹智宇,肖关丽.干旱胁迫对冬马铃薯苗期生理指标及光合特性的影响[J].云南农业大学学报(自然科学),2017,32(6):992.DOI:1 0.16211/j.issn.1004-390X(n).2017.06.005.
[10]YOUNIS M E,HASANEEN M N A,ABDELAZIZ H MM.An enhancing effect of visible light and UV radiation on phenolic compounds and various antioxidants in broad bean seedlings[J].Plant Signaling&Behavior,2010,5(10):1197.DOI:10.4161/psb.5.10.11978.
[11]赵冰,李宏.60Co-γ射线处理对菜用枸杞的影响[J].首都师范大学学报(自然科学版),2008,29(2):31.
[12]郑凤霞.低剂量60Co-γ辐照对拟南芥幼苗耐热性的影响[D].新乡:河南师范大学,2016.
[13]廖安红,陈红.不同60Co-γ射线辐照剂量对刺梨幼苗生长及生理特性的影响[J].分子植物育种,2016,14(3):742.DOI:10.13271/j.mpb.014.000742.
[14]滕娟,熊俊芬,何忠俊,等.60Co-γ辐射对三七幼苗生理特性的影响[J].云南农业大学学报(自然科学),2015,30(3):445.DOI:10.16211/j.issn.1004-390X(n).2015.03.020.
[15]丁佳红,薛正莲,杨超英.水杨酸对铜胁迫下水稻幼苗膜脂过氧化作用的影响[J].黑龙江农业科学,2013(1):14.DOI:10.3969/j.issn.1002-2767.2013.01.007.
[16]熊秋芳,陈玉霞,张雪清,等.60Co-γ辐射对萝卜种子萌发和幼苗生长的影响[J].浙江农业科学,2014,1(3):356.DOI:10.16178/j.issn.0528-9017.2014.03.033.
[17]张玉,白史且,李达旭,等.60Co-γ辐射对菊苣种子发芽及幼苗生理的影响[J].草地学报,2013,21(1):147.
[18]陈斌,张传玲,江红生,等.纳米银诱导拟南芥活性氧自由基的积累和抗氧化系统的改变[J].基因组学与应用生物学,2017(4):1646.DOI:10.13417/j.gab.036.001646.
[19]廖飞雄,潘瑞炽.60Co-γ辐射菜心种子对苗期细胞膜及保护酶活性的影响[J].核农学报,2001,15(5):286.
[20]王月华,韩烈保,尹淑霞,等.60Co-γ射线辐射对早熟禾种子发芽及种子内酶活性的影响[J].中国草地学报,2006,28(1):54.