基于ISSR-PCR的长白落叶松种子园单株遗传多样性分析
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摘要
以长白落叶松无性系种子园347个单株为试验材料,利用ISSR-PCR分子标记技术分析其遗传多样性的结果表明,长白落叶松种子园单株具有丰富的遗传多样性。11条引物共扩增出172条谱带,其中,多态性谱带169条,多态位点比率为98.26%,基因多样性指数0.285 5,Shannon多样性指数0.479 5～0.520 4,Nei's指数0.064 0～0.436 0。
With 347 single plant of Larix olgensis in the Seed Orchard, we studied the genetic diversity by ISSR markers. The results showsed that Larix olgensis in Seed Orchard has abundant genetic diversity. 11 primers extend 172 polymorphic locis, and there are 169 polymorphic locis, whose average polymorphism percentage was 98.26%. The average Nei's gene diversity index was 0.285 5. The variation range of the Shannon diversity index of the 347 samples was 0.479 5～0520 4 and the The variation range of Nei's gene diversity index was 0.064～0.436 0.
With 347 single plant of Larix olgensis in the Seed Orchard, we studied the genetic diversity by ISSR markers. The results showsed that Larix olgensis in Seed Orchard has abundant genetic diversity. 11 primers extend 172 polymorphic locis, and there are 169 polymorphic locis, whose average polymorphism percentage was 98.26%. The average Nei's gene diversity index was 0.285 5. The variation range of the Shannon diversity index of the 347 samples was 0.479 5～0520 4 and the The variation range of Nei's gene diversity index was 0.064～0.436 0.
引文
[1] Soltis PS, Slotis DE. Genetics variation in endemidc and widespread plant species:Examples from Szxifragaceae and Polystichum. Aliso, 1991, 13:215-223.
[2]林萍,张含国,谢运海.正交设计优化落叶松ISSR-PCR反应体系.生物技术, 2005, 15(5):34-37.
[3]马丽,侯乐峰,郝兆祥,等. 82个石榴品种遗传多样性的ISSR分析[J].果树学报, 2015, 32(5):741-750.
[4] Akhare A,Sakhare S,Kulwal P,et al.RAPD profile studies in Sorghum for identification of hybrids and their parents[J].Int J Inter Biol,2008,3(1):18-24.
[5]钱韦,葛颂,洪德元.采用RAPD和ISSR标记探讨中国疣粒野生稻的遗传多样性[J].植物学报,2000,42:741.
[6] Ge X J, Sun M. Reprodutive biology and genetic diversity of a cryptoviviparous mangrove Aegiceras corniculatum(Myrtinaceae)using allozyme and intersimple sequence repeat(ISSR)analysis[J]. Mol E col, 1999, 8:2 061.
[7]胡连清,王清明,丁显平,等.极危物种———南川木波罗遗传多样性ISSR分析[J].四川大学学报(自然科学版), 2018,55(04):865-872.
[8] Camacho F J, Liston A. Population structure and genetic diversity of Botrychium pumicola(Ophioglossaceae)based on inter-simple sequence repeats(ISSR)[J]. Am J Bot,2001, 88:1 065.
[9]郭艳,洪建聪,陈铌铍,等.白及遗传多样性的ISSR分析[J].中成药, 2018, 40(09):2 020-2 024.
[10]姚宇,张含国,张振,等.去劣疏伐对长白落叶松初级无性系种子园SSR遗传多样性的影响[J].中南林业科技大学学报, 2013, 33(03):40-46.
[11]于大德.华北落叶松种子园遗传多样性及亲本分析[D].北京林业大学, 2014.
[12]范英明.华北落叶松天然群体SSR标记遗传多样性分析[D].北京林业大学, 2014.
[2]林萍,张含国,谢运海.正交设计优化落叶松ISSR-PCR反应体系.生物技术, 2005, 15(5):34-37.
[3]马丽,侯乐峰,郝兆祥,等. 82个石榴品种遗传多样性的ISSR分析[J].果树学报, 2015, 32(5):741-750.
[4] Akhare A,Sakhare S,Kulwal P,et al.RAPD profile studies in Sorghum for identification of hybrids and their parents[J].Int J Inter Biol,2008,3(1):18-24.
[5]钱韦,葛颂,洪德元.采用RAPD和ISSR标记探讨中国疣粒野生稻的遗传多样性[J].植物学报,2000,42:741.
[6] Ge X J, Sun M. Reprodutive biology and genetic diversity of a cryptoviviparous mangrove Aegiceras corniculatum(Myrtinaceae)using allozyme and intersimple sequence repeat(ISSR)analysis[J]. Mol E col, 1999, 8:2 061.
[7]胡连清,王清明,丁显平,等.极危物种———南川木波罗遗传多样性ISSR分析[J].四川大学学报(自然科学版), 2018,55(04):865-872.
[8] Camacho F J, Liston A. Population structure and genetic diversity of Botrychium pumicola(Ophioglossaceae)based on inter-simple sequence repeats(ISSR)[J]. Am J Bot,2001, 88:1 065.
[9]郭艳,洪建聪,陈铌铍,等.白及遗传多样性的ISSR分析[J].中成药, 2018, 40(09):2 020-2 024.
[10]姚宇,张含国,张振,等.去劣疏伐对长白落叶松初级无性系种子园SSR遗传多样性的影响[J].中南林业科技大学学报, 2013, 33(03):40-46.
[11]于大德.华北落叶松种子园遗传多样性及亲本分析[D].北京林业大学, 2014.
[12]范英明.华北落叶松天然群体SSR标记遗传多样性分析[D].北京林业大学, 2014.