盐度及pH突变对大竹蛏稚贝抗氧化酶活力的影响
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摘要
为了解盐度、pH对大竹蛏Solen grandis稚贝抗氧化酶活力的影响,在不同盐度(15、20、30、35)和pH(7.0、7.5、8.5、9.0)突变下,分别在0、4、12、24、48、72、96 h测定了壳长为(1.33±0.10)cm大竹蛏稚贝超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活力的变化。结果表明:盐度20组12、24h及盐度30组24 h,大竹蛏稚贝SOD活力显著高于对照组(盐度25)(P<0.05),盐度20组4、12 h和盐度30组12、24 h时,大竹蛏稚贝CAT活力显著高于对照组(P<0.05),48~96 h时,盐度20、盐度30组的两种抗氧化酶活力逐渐恢复至对照组水平;盐度15、35组48~96 h时,大竹蛏稚贝的SOD和CAT活力显著高于对照组(P<0.05),pH 7.5、pH 8.5组12~96 h时,大竹蛏稚贝的SOD和CAT活力显著高于对照组(pH 8.0)(P<0.05)(除pH 8.5组48 h外);pH 7.0、pH 9.0组12~96 h时,其SOD活力显著高于对照组(P<0.05),pH 7.0和pH 9.0组72 h和96 h时,CAT活力显著低于对照组(P<0.05)。研究表明:盐度和pH突变对大竹蛏稚贝SOD和CAT活力均有影响,大竹蛏稚贝对盐度20和盐度30突变有一定适应性;盐度15、盐度35和pH 7.5、pH 8.5突变后,大竹蛏稚贝长时间处于氧化应激状态;pH 7.0和pH 9.0突变可能会造成大竹蛏稚贝细胞氧化损伤;生产实践中,大竹蛏稚贝培育的盐度和pH范围分别控制在20~30和7.5~8.5较为合适。
Superoxide dismutase( SOD) and catalase( CAT) activities of juvenile razor clam Solen grandis with shell length of(1. 33 cm±0. 10 cm) were monitored at a salinity of 15,20,30 and 35 and pH of 7.0,7.5,8.5 and9.0 for 0,4,12,24,48,72 and 96 h,respectively,in order to clarify the effects of salinity and pH on the antioxidant enzyme activities of juvenile razor clam. The results showed that there were significantly higher activities of SOD in the clam exposed to a salinity of 20 for 12 and 24 h and a salinity of 30 for 24 h than those in the control group( salinity 25)( P<0.05). However,there were significantly higher CAT activities in the clam exposed to a salinity of 20 for 4 and 12 h and a salinity of 30 for 12 and 24 h than those in the control group( salinity 25)( P<0.05),finally activities of both SOD and CAT being recovered to the control level in 48 h and 96 h. The razor clam exposed to a salinity of 15 and 35 from 48 h to 96 h had significantly higher SOD and CAT activities than those in controls( P<0.05) did. The razor clam exposed to pH 7.5 and pH 8.5 from 12 h to 96 h had significantly higher SOD and CAT activities than those in control group did( pH 8.0,except pH 8.5 in 48 h)( P < 0.05). There was significantly higher SOD activity in the clam exposed to pH 7.0 and pH 9.0 than those in the control group at 12-96h( P<0.05),the activities of CAT being significantly lower from 72 h to 96 h( P <0.05). The findings indicated that SOD and CAT activities in razor clam were affected by changes in salinity and pH,the razor clam being adaptable to sudden change in salinity within 20 and 30,and that the razor clam showed cellular oxidative damage induced by the pH 7.0 and pH 9.0 changes under the status of oxidative stress for a long period of exposure at a salinity of 15 and 35 and pH 7.5,and 8.5. The adaptive salinity and pH are suggested to should be kept in 20-30 and7.5-8.5 for the culture of Solen grandis juveniles,respectively.
Superoxide dismutase( SOD) and catalase( CAT) activities of juvenile razor clam Solen grandis with shell length of(1. 33 cm±0. 10 cm) were monitored at a salinity of 15,20,30 and 35 and pH of 7.0,7.5,8.5 and9.0 for 0,4,12,24,48,72 and 96 h,respectively,in order to clarify the effects of salinity and pH on the antioxidant enzyme activities of juvenile razor clam. The results showed that there were significantly higher activities of SOD in the clam exposed to a salinity of 20 for 12 and 24 h and a salinity of 30 for 24 h than those in the control group( salinity 25)( P<0.05). However,there were significantly higher CAT activities in the clam exposed to a salinity of 20 for 4 and 12 h and a salinity of 30 for 12 and 24 h than those in the control group( salinity 25)( P<0.05),finally activities of both SOD and CAT being recovered to the control level in 48 h and 96 h. The razor clam exposed to a salinity of 15 and 35 from 48 h to 96 h had significantly higher SOD and CAT activities than those in controls( P<0.05) did. The razor clam exposed to pH 7.5 and pH 8.5 from 12 h to 96 h had significantly higher SOD and CAT activities than those in control group did( pH 8.0,except pH 8.5 in 48 h)( P < 0.05). There was significantly higher SOD activity in the clam exposed to pH 7.0 and pH 9.0 than those in the control group at 12-96h( P<0.05),the activities of CAT being significantly lower from 72 h to 96 h( P <0.05). The findings indicated that SOD and CAT activities in razor clam were affected by changes in salinity and pH,the razor clam being adaptable to sudden change in salinity within 20 and 30,and that the razor clam showed cellular oxidative damage induced by the pH 7.0 and pH 9.0 changes under the status of oxidative stress for a long period of exposure at a salinity of 15 and 35 and pH 7.5,and 8.5. The adaptive salinity and pH are suggested to should be kept in 20-30 and7.5-8.5 for the culture of Solen grandis juveniles,respectively.
引文
[1]Guerra C,Zenteno-Savín T,Maeda-Martínez A N,et al.The effect of predator exposure and reproduction on oxidative stress parameters in the Catarina scallop Argopecten ventricosus[J].Comparative Biochemistry&Physiology Part A:Molecular&Integrative Physiology,2013,165(1):89-96.
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[3]Ghosh J,Myers C E.Inhibition of arachidonate 5-lipoxygenase triggers massive apoptosis in human prostate cancer cells[J].Proceedings of the National Academy of Sciences of the United States of America,1998,95(22):13182-13187.
[4]尹光耀,尹玉芬,何雪芬,等.驻春丸对肾阳虚老年人免疫与内分泌功能的影响[J].中国中西医结合杂志,1995,15(10):601-603.
[5]Lee Y J,Galoforo S S,Berns C M,et al.Glucose deprivation-induced cytotoxicity and alterations in mitogen-activated protein kinase activation are mediated by oxidative stress in multidrug-resistant human breast carcinoma cells[J].Journal of Biological Chemistry,1998,273(9):5294-5299.
[6]Barja G.Aging in vertebrates,and the effect of caloric restriction:a mitochondrial free radical production-DNA damage mechanism?[J].Biological Reviews,2004,79(2):235-251.
[7]Kim B M,Rhee J S,Park G S,et al.Cu/Zn-and Mn-superoxide dismutase(SOD)from the copepod Tigriopus japonicus:molecular cloning and expression in response to environmental pollutants[J].Chemosphere,2011,84(10):1467-1475.
[8]杨细兰.褶纹冠蚌过氧化氢酶基因克隆、原核表达及酶活性分析[D].南昌:南昌大学,2011.
[9]Fridovich I.Superoxide radical and superoxide dismutases[J].Annual Review of Biochemistry,1995,64(1):97-112.
[10]Ekanayake P M,De Zoysa M,Kang H S,et al.Cloning,characterization and tissue expression of disk abalone(Haliotis discus discus)catalase[J].Fish&Shellfish Immunology,2008,24(3):267-278.
[11]袁春营,崔青曼.盐度影响水产养殖动物生长发育的研究进展[J].水产科学,2004,23(5):41-42.
[12]于天基,李健,李吉涛,等.p H胁迫对脊尾白虾抗氧化酶活力的影响[J].海洋科学,2015,39(5):47-53.
[13]Tomanek L,Zuzow M J,Ivanina A V,et al.Proteomic response to elevated PCO2level in eastern oysters,Crassostrea virginica:evidence for oxidative stress[J].Journal of Experimental Biology,2011,214(11):1836-1844.
[14]邓平平,施永梅,汪洋,等.盐度对长江刀鲚幼鱼非特异性免疫酶和消化酶活性的影响[J].大连海洋大学学报,2016,31(5):533-537.
[15]边平江,邱成功,徐善良,等.盐度对暗纹东方鲀生长、非特异性免疫和抗氧化酶活力的影响[J].水生生物学报,2014,38(1):108-114.
[16]吕昊泽,刘健,陈锦辉,等.盐度对缢蛏超氧化物歧化酶和过氧化氢酶活性的影响[J].海洋渔业,2013,35(4):474-478.
[17]Hu M H,Li L S,Sui Y M,et al.Effect of p H and temperature on antioxidant responses of the thick shell mussel Mytilus coruscus[J].Fish&Shellfish Immunology,2015,46(2):573-583.
[18]杨丽娟,游育红.细胞受到氧化应激后损伤的检测方法[J].医学综述,2010,16(6):924-927.
[19]张滔,刘相全,孙振兴,等.大竹蛏(Solen grandis)不同地理群体遗传多样性的AFLP分析[J].海洋与湖沼,2013,44(2):525-530.
[20]张志伟,陈爱华,张志勇,等.大竹蛏5个野生群体遗传多样性的微卫星分析[J].中国水产科学,2015,22(4):740-748.
[21]宋贤亭,于瑞海,马培振,等.大竹蛏室内人工育苗技术研究[J].海洋湖沼通报,2015(4):56-60.
[22]SaràG,Romano C,Widdows J,et al.Effect of salinity and temperature on feeding physiology and scope for growth of an invasive species(Brachidontes pharaonis-Mollusca:Bivalvia)within the Mediterranean sea[J].Journal of Experimental Marine Biology and Ecology,2008,363(1-2):130-136.
[23]范德朋,潘鲁青,马甡,等.盐度和p H对缢蛏耗氧率及排氨率的影响[J].中国水产科学,2002,9(3):234-238.
[24]Pillai B R,Diwan A D.Effects of acute salinity stress on oxygen consumption and ammonia excretion rates of the marine shrimp Metapenaeus monoceros[J].Journal of Crustacean Biology,2002,22(1):45-52.
[25]Choi C Y,An K W,An M I.Molecular characterization and m RNA expression of glutathione peroxidase and glutathione S-transferase during osmotic stress in olive flounder(Paralichthys olivaceus)[J].Comparative Biochemistry and Physiology Part A:Molecular&Integrative Physiology,2008,149(3):330-337.
[26]Holmblad T,Sderhll K.Cell adhesion molecules and antioxidative enzymes in a crustacean,possible role in immunity[J].Aquaculture,1999,172(1-2):111-123.
[27]Wang W N,Wu J,Su S J.Effects of salinity stress on antioxidant enzymes of Penaeus monodon of two different life stages[J].Comparative Biochemistry and Physiology Part C:Toxicology&Pharmacology,2008,148(4):466.
[28]刘美剑,常亚青,杨芸菲,等.盐度渐变对虾夷扇贝(Patinopecten yessoensis)免疫指标的影响[J].中国农业科技导报,2011,13(3):129-135.
[29]周东,母昌考,宋微微,等.低盐胁迫对三疣梭子蟹组织中抗氧化酶和ATP酶活力的影响[J].生态科学,2014,33(4):698-703.
[30]王晓杰,张秀梅,李文涛.盐度胁迫对许氏平#血液免疫酶活力的影响[J].渔业科学进展,2005,26(6):17-21.
[31]李子牛,林听听,么宗利,等.盐度对青蛤抗氧化酶活性及生长的影响[J].生态学杂志,2012,31(10):2625-2630.
[32]陈爱华,姚国兴,张志伟,等.温度、盐度和底质对大竹蛏稚贝生长及存活的影响[J].热带海洋学报,2010,29(5):94-97.
[33]樊甄姣,杨爱国,刘志鸿,等.p H对栉孔扇贝体内几种免疫因子的影响[J].中国水产科学,2006,13(4):650-654.
[34]曾媛媛,艾春香,刘建国,等.p H胁迫对拟穴青蟹体内几种免疫因子的影响[J].厦门大学学报:自然科学版,2008,47(3):413-418.
[35]文春根,张丽红,胡宝庆,等.p H对背角无齿蚌(Anodonta woodiana)5种免疫因子的影响[J].南昌大学学报:理科版,2009,33(2):172-176.
[36]Matozzo V,Chinellato A,Munari M,et al.Can the combination of decreased p H and increased temperature values induce oxidative stress in the clam Chamelea gallina and the mussel Mytilus galloprovincialis?[J].Marine Pollution Bulletin,2013,72(1):34-40.
[2]Lambeth J D.NOX enzymes and the biology of reactive oxygen[J].Nature Reviews Immunology,2004,4(3):181-189.
[3]Ghosh J,Myers C E.Inhibition of arachidonate 5-lipoxygenase triggers massive apoptosis in human prostate cancer cells[J].Proceedings of the National Academy of Sciences of the United States of America,1998,95(22):13182-13187.
[4]尹光耀,尹玉芬,何雪芬,等.驻春丸对肾阳虚老年人免疫与内分泌功能的影响[J].中国中西医结合杂志,1995,15(10):601-603.
[5]Lee Y J,Galoforo S S,Berns C M,et al.Glucose deprivation-induced cytotoxicity and alterations in mitogen-activated protein kinase activation are mediated by oxidative stress in multidrug-resistant human breast carcinoma cells[J].Journal of Biological Chemistry,1998,273(9):5294-5299.
[6]Barja G.Aging in vertebrates,and the effect of caloric restriction:a mitochondrial free radical production-DNA damage mechanism?[J].Biological Reviews,2004,79(2):235-251.
[7]Kim B M,Rhee J S,Park G S,et al.Cu/Zn-and Mn-superoxide dismutase(SOD)from the copepod Tigriopus japonicus:molecular cloning and expression in response to environmental pollutants[J].Chemosphere,2011,84(10):1467-1475.
[8]杨细兰.褶纹冠蚌过氧化氢酶基因克隆、原核表达及酶活性分析[D].南昌:南昌大学,2011.
[9]Fridovich I.Superoxide radical and superoxide dismutases[J].Annual Review of Biochemistry,1995,64(1):97-112.
[10]Ekanayake P M,De Zoysa M,Kang H S,et al.Cloning,characterization and tissue expression of disk abalone(Haliotis discus discus)catalase[J].Fish&Shellfish Immunology,2008,24(3):267-278.
[11]袁春营,崔青曼.盐度影响水产养殖动物生长发育的研究进展[J].水产科学,2004,23(5):41-42.
[12]于天基,李健,李吉涛,等.p H胁迫对脊尾白虾抗氧化酶活力的影响[J].海洋科学,2015,39(5):47-53.
[13]Tomanek L,Zuzow M J,Ivanina A V,et al.Proteomic response to elevated PCO2level in eastern oysters,Crassostrea virginica:evidence for oxidative stress[J].Journal of Experimental Biology,2011,214(11):1836-1844.
[14]邓平平,施永梅,汪洋,等.盐度对长江刀鲚幼鱼非特异性免疫酶和消化酶活性的影响[J].大连海洋大学学报,2016,31(5):533-537.
[15]边平江,邱成功,徐善良,等.盐度对暗纹东方鲀生长、非特异性免疫和抗氧化酶活力的影响[J].水生生物学报,2014,38(1):108-114.
[16]吕昊泽,刘健,陈锦辉,等.盐度对缢蛏超氧化物歧化酶和过氧化氢酶活性的影响[J].海洋渔业,2013,35(4):474-478.
[17]Hu M H,Li L S,Sui Y M,et al.Effect of p H and temperature on antioxidant responses of the thick shell mussel Mytilus coruscus[J].Fish&Shellfish Immunology,2015,46(2):573-583.
[18]杨丽娟,游育红.细胞受到氧化应激后损伤的检测方法[J].医学综述,2010,16(6):924-927.
[19]张滔,刘相全,孙振兴,等.大竹蛏(Solen grandis)不同地理群体遗传多样性的AFLP分析[J].海洋与湖沼,2013,44(2):525-530.
[20]张志伟,陈爱华,张志勇,等.大竹蛏5个野生群体遗传多样性的微卫星分析[J].中国水产科学,2015,22(4):740-748.
[21]宋贤亭,于瑞海,马培振,等.大竹蛏室内人工育苗技术研究[J].海洋湖沼通报,2015(4):56-60.
[22]SaràG,Romano C,Widdows J,et al.Effect of salinity and temperature on feeding physiology and scope for growth of an invasive species(Brachidontes pharaonis-Mollusca:Bivalvia)within the Mediterranean sea[J].Journal of Experimental Marine Biology and Ecology,2008,363(1-2):130-136.
[23]范德朋,潘鲁青,马甡,等.盐度和p H对缢蛏耗氧率及排氨率的影响[J].中国水产科学,2002,9(3):234-238.
[24]Pillai B R,Diwan A D.Effects of acute salinity stress on oxygen consumption and ammonia excretion rates of the marine shrimp Metapenaeus monoceros[J].Journal of Crustacean Biology,2002,22(1):45-52.
[25]Choi C Y,An K W,An M I.Molecular characterization and m RNA expression of glutathione peroxidase and glutathione S-transferase during osmotic stress in olive flounder(Paralichthys olivaceus)[J].Comparative Biochemistry and Physiology Part A:Molecular&Integrative Physiology,2008,149(3):330-337.
[26]Holmblad T,Sderhll K.Cell adhesion molecules and antioxidative enzymes in a crustacean,possible role in immunity[J].Aquaculture,1999,172(1-2):111-123.
[27]Wang W N,Wu J,Su S J.Effects of salinity stress on antioxidant enzymes of Penaeus monodon of two different life stages[J].Comparative Biochemistry and Physiology Part C:Toxicology&Pharmacology,2008,148(4):466.
[28]刘美剑,常亚青,杨芸菲,等.盐度渐变对虾夷扇贝(Patinopecten yessoensis)免疫指标的影响[J].中国农业科技导报,2011,13(3):129-135.
[29]周东,母昌考,宋微微,等.低盐胁迫对三疣梭子蟹组织中抗氧化酶和ATP酶活力的影响[J].生态科学,2014,33(4):698-703.
[30]王晓杰,张秀梅,李文涛.盐度胁迫对许氏平#血液免疫酶活力的影响[J].渔业科学进展,2005,26(6):17-21.
[31]李子牛,林听听,么宗利,等.盐度对青蛤抗氧化酶活性及生长的影响[J].生态学杂志,2012,31(10):2625-2630.
[32]陈爱华,姚国兴,张志伟,等.温度、盐度和底质对大竹蛏稚贝生长及存活的影响[J].热带海洋学报,2010,29(5):94-97.
[33]樊甄姣,杨爱国,刘志鸿,等.p H对栉孔扇贝体内几种免疫因子的影响[J].中国水产科学,2006,13(4):650-654.
[34]曾媛媛,艾春香,刘建国,等.p H胁迫对拟穴青蟹体内几种免疫因子的影响[J].厦门大学学报:自然科学版,2008,47(3):413-418.
[35]文春根,张丽红,胡宝庆,等.p H对背角无齿蚌(Anodonta woodiana)5种免疫因子的影响[J].南昌大学学报:理科版,2009,33(2):172-176.
[36]Matozzo V,Chinellato A,Munari M,et al.Can the combination of decreased p H and increased temperature values induce oxidative stress in the clam Chamelea gallina and the mussel Mytilus galloprovincialis?[J].Marine Pollution Bulletin,2013,72(1):34-40.