不同表面活性剂对赤霉素在苹果角质膜渗透的影响
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摘要
[目的]探讨不同表面活性剂的使用效果,筛选出高效复合型的表面活性剂。[方法]在20、25和30℃条件下选用辛二酸二乙酯(DESU)、磷酸三丁酯(TBP)和癸二酸二乙酯(DES)3种不同浓度表面活性剂及其不同配方的复合型表面活性剂,进行了离体苹果角质膜渗透试验,研究不同表面活性剂对赤霉素在角质膜渗透吸收的影响。[结果]TBP、DES和DESU及其混合型的表面活性剂都对赤霉素在苹果果实离体角质膜上的渗透有明显的促进作用。其中,DESU的促进作用高于TBP和DES,是对照组效果的5.27倍,使用的最佳浓度为300 mg/L;DES和TBP作用效果分别是对照组的4.87和3.97倍,最佳使用浓度分别为180和60 mg/L;混合型的表面活性剂TBP+DESU(浓度为60 mg/L+300 mg/L)对赤霉素的渗透促进作用最显著,是对照组的7.39倍。[结论]该研究结果为植物微肥产品的研发、生产和使用提供了理论依据。
[Objective] The aim was to explore the use effect of different surfactants and screen out effective compound surfactant.[Method] A high-efficiency compound surfactant was selected to explore the effect of different surfactants,the cutin membranes of apple were sprayed with three kinds of surfactants of tributyl phosphate( TBP),diethyl sebacate( DES) and diethyl suberate( DESU) respectively and the their mixed surfactants to study the effect on gibberellin( GA) absorption at 20,25 and 30 ℃.[Result] The TBP,DES and DESU surfactant and its hybrid watch all of gibberellin on apple fruit in vitro cutin membrane permeability had obvious promoting effect,including DESU promoting effect was higher than TBP and DES,5.27 times that of the control effect.The optimum concentration was 300 mg/L; the effects of DES and TBP were 4.87 times and 3.97 times as that of control group,and the optimal concentration was 180 and 60 mg/L respectively.The mixed surfactant TBP +DESU( concentration of 60 mg/L+300 mg/L) had the most significant effect on the osmotic effect of erythromycin,which was 7.39 times of the control group.[Conclusion] The result provides theoretical basis for research,production and use of plant microfertilizer products.
[Objective] The aim was to explore the use effect of different surfactants and screen out effective compound surfactant.[Method] A high-efficiency compound surfactant was selected to explore the effect of different surfactants,the cutin membranes of apple were sprayed with three kinds of surfactants of tributyl phosphate( TBP),diethyl sebacate( DES) and diethyl suberate( DESU) respectively and the their mixed surfactants to study the effect on gibberellin( GA) absorption at 20,25 and 30 ℃.[Result] The TBP,DES and DESU surfactant and its hybrid watch all of gibberellin on apple fruit in vitro cutin membrane permeability had obvious promoting effect,including DESU promoting effect was higher than TBP and DES,5.27 times that of the control effect.The optimum concentration was 300 mg/L; the effects of DES and TBP were 4.87 times and 3.97 times as that of control group,and the optimal concentration was 180 and 60 mg/L respectively.The mixed surfactant TBP +DESU( concentration of 60 mg/L+300 mg/L) had the most significant effect on the osmotic effect of erythromycin,which was 7.39 times of the control group.[Conclusion] The result provides theoretical basis for research,production and use of plant microfertilizer products.
引文
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[10]BAUR P,GRAYSON B T,SCHONHERR J.Polydisperse ethoxylated fatty alcohol surfactants as accelerators of cuticular penetration.1.Effects of ethoxy chain length and the size of the penetrants[J].Pest management science,1997,51(2):131-152.
[11]王保勤,牛吉山.一种小麦子粒赤霉素含量测定方法的建立[J].中国农学通报,2007,23(1):334-337.
[12]SHI T,SIMANOVA E,SCHONHERR J,et al.Effects of accelerators on mobility of14C-2,4-dichlorophenoxy butyric acid in plant cuticles depends on type and concentration of accelerator[J].Journal of agricultural and food chemistry,2005,53(6):2207-2212.
[2]JENKS M A,JOLY R J,PETERS P J,et al.Chemically induced cuticle mutation affecting epidermal conductance to water vapor and disease susceptibility in Sorghum bicolor(L.)Moench.[J].Plant physiology,1994,105(4):1239-1245.
[3]RIEDERER M,MULLER C.Biology of the plant cuticle[J].Systematic botany,2006,33(3):613.
[4]RIEDERER M,SCHREIBER L.Protecting against water loss:Analysis of the barrier properties of plant cuticles[J].Journal of experimental botany,2001,52(363):2023-2032.
[5]谭婷婷,郝姗姗,赵莉,等.表面活性剂的性能与应用(ⅪⅤ)——表面活性剂的润湿作用及其应用[J].日用化学工业,2015,45(2):72-75,89.
[6]颜燕.表面活性剂的应用[J].考试周刊,2012(77):153.
[7]华乃震,林雨佳.用于农药的表面活性剂新进展[J].江苏化工,2000(4):20-21.
[8]徐燕莉.表面活性剂的功能[M].北京:化学工业出版社,2000.
[9]蒋超,卢天成,李毅丹,等.赤霉素在非生物胁迫中的作用[J].生物技术通报,2016,32(5):11-15.
[10]BAUR P,GRAYSON B T,SCHONHERR J.Polydisperse ethoxylated fatty alcohol surfactants as accelerators of cuticular penetration.1.Effects of ethoxy chain length and the size of the penetrants[J].Pest management science,1997,51(2):131-152.
[11]王保勤,牛吉山.一种小麦子粒赤霉素含量测定方法的建立[J].中国农学通报,2007,23(1):334-337.
[12]SHI T,SIMANOVA E,SCHONHERR J,et al.Effects of accelerators on mobility of14C-2,4-dichlorophenoxy butyric acid in plant cuticles depends on type and concentration of accelerator[J].Journal of agricultural and food chemistry,2005,53(6):2207-2212.