荧光探针法测定十六烷基三甲基溴化铵临界胶束浓度
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摘要
阳离子表面活性剂十六烷基三甲基溴化铵(CTAB)是一种具有长链烷基的季铵盐,可用于纳米粒子合成、DNA提取与家庭洗护等。临界胶束浓度(CMC)是表面活性剂溶液性质发生显著变化的分水岭,准确测量其临界胶束浓度有助于更好的发挥该化合物增溶、杀菌等作用。将4H,4'H-2,2'-双色烯-4,4'-二酮(BCDO)作为荧光探针,建立了以荧光探针法测定表面活性剂的临界胶束浓度的新方法,测定了典型阳离子表面活性剂十六烷基三甲基溴化铵的临界胶束浓度。探针用量8. 70×10~(-6)g的条件下,配制11个浓度分别为0. 10、0. 20、0. 30、0. 40、0. 50、0. 60、0. 70、0. 80、0. 90、1. 00、1. 10mmol/L的BCDO-CTAB样品溶液,分别测定样品溶液的荧光光谱。由BCDO-CTAB样品荧光强度-浓度曲线可知,曲线出现拐点时对应的浓度为0. 80 mmol/L,此浓度与CTAB临界胶束浓度的文献值一致,表明BCDO作为荧光探针测定CTAB的CMC是可行的。
Cationic surfactant cetyl trimethyl ammonium bromide( CTAB) is a quaternary ammonium salt with a long-chain alkyl group.It was used for nanoparticle synthesis,DNA extraction and household washing.When the concentration of surface active agent is close to critical micelle concentration( CMC),the qualities of surface active agent will change strikingly.Accurate measurement of compounds' critical micelle concentration helps in solubilization,sterilization and so on. Using 4 H,4'H-2,2'-bichromene-4,4'-dione( BCDO) as a fluorescent probe,a novel fluorescence probe method was established for determination of critical micelle concentration of a typical cationic surfactant CTAB.As only 8. 70×10~(-6) g fluorescent probe,11 sample solutions of BCDO-CTAB were prepared with the concentration of 0. 10,0. 20,0. 30,0. 40,0. 50,0. 60,0. 70,0. 80,0. 90,1. 00,1. 10 mmol/L separately to obtain their fluorescence spectrometries.From the fluorescence intensity-concentration curve of BCDO-CTAB sample,the corresponding concentration at the inflection point of the curve was 0. 80 mmol/L.This concentration was consistent with the literature value of the critical micelle concentration of CTAB.It indicated that BCDO as a fluorescent probe for the determination of CMC of CTAB was feasible.
Cationic surfactant cetyl trimethyl ammonium bromide( CTAB) is a quaternary ammonium salt with a long-chain alkyl group.It was used for nanoparticle synthesis,DNA extraction and household washing.When the concentration of surface active agent is close to critical micelle concentration( CMC),the qualities of surface active agent will change strikingly.Accurate measurement of compounds' critical micelle concentration helps in solubilization,sterilization and so on. Using 4 H,4'H-2,2'-bichromene-4,4'-dione( BCDO) as a fluorescent probe,a novel fluorescence probe method was established for determination of critical micelle concentration of a typical cationic surfactant CTAB.As only 8. 70×10~(-6) g fluorescent probe,11 sample solutions of BCDO-CTAB were prepared with the concentration of 0. 10,0. 20,0. 30,0. 40,0. 50,0. 60,0. 70,0. 80,0. 90,1. 00,1. 10 mmol/L separately to obtain their fluorescence spectrometries.From the fluorescence intensity-concentration curve of BCDO-CTAB sample,the corresponding concentration at the inflection point of the curve was 0. 80 mmol/L.This concentration was consistent with the literature value of the critical micelle concentration of CTAB.It indicated that BCDO as a fluorescent probe for the determination of CMC of CTAB was feasible.
引文
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[13]章苏宁,张健,宋晓秋,等.稳态荧光探针法测定Tween系列非离子表面活性剂临界胶束浓度[J].光谱实验室,2010,27(4):1 231-1 236.
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[15]杨涛,李文娟,周从山.芘荧光探针光谱法测定CTAB临界胶束浓度[J].石化技术与应用,2007,25(1):48-51.
[2]王宝辉,张学佳,纪巍,等.表面活性剂环境危害性分析[J].化工进展,2007,26(9):1 263-1 268.
[3]马文辉,孙丽微,徐群,等.纯水中表面活性剂促进的氰离子探针[J].化学试剂,2013,35(2):157-159.
[4]郑薇.表面活性剂工业的技术进展[J].化工进展,2003,22(4):329-335.
[5]王岩,王晶,卢方正,等.十二烷基硫酸钠临界胶束浓度测定实验的探讨[J].实验室科学,2012,15(3):70-72.
[6]KUMAR B,TIKRIHA D,GHOSH K K,et al. Effect of polymers and temperature on critical micelle concentration of some gemini and monomeric surfactants[J]. J.Chem.Thermodyn.,2013,62(7):178-185.
[7]李云峰,相明辉,于洋,等.共振光散射法测定复配表面活性剂的临界胶束浓度[J].科技通报,2016,32(8):1-4.
[8]SCHOLZ N,BEHNKE T,RESH-GENGER U.Determination of the critical micelle concentration of neutral and ionic surfactants with fluorometr[J]. J. Fluoresc.,2018,28(1):465-476.
[9]王新红,顾云兰,戴兢陶.二元阳离子表面活性剂的CMC及相互作用[J].材料导报,2012,26(2):102-105.
[10]高凤凤.表面活性剂在复杂界面及体相行为的理论研究[D].山东:山东大学,2015:21-24.
[11]任学贞,李干佐,王弘立,等.荧光探针法测定甜菜碱CMC的研究[J].高等学校化学学报,1995,16(8):1 295-1 297.
[12]张萍,吴玉芹,李玲,等.籍疏水花菁染料的荧光突变测定非离子表面活性剂临界胶束浓度[J].安徽师范大学学报,2005,28(1):68-71.
[13]章苏宁,张健,宋晓秋,等.稳态荧光探针法测定Tween系列非离子表面活性剂临界胶束浓度[J].光谱实验室,2010,27(4):1 231-1 236.
[14]GABRIEL S,LEUPOLD E. Umwandlungen des aethindiphtalids[J]. Ber. Dtsch. Chem. Ges.,1989,31(1):1 159-1 174.
[15]杨涛,李文娟,周从山.芘荧光探针光谱法测定CTAB临界胶束浓度[J].石化技术与应用,2007,25(1):48-51.