响应面法对剩余污泥发酵过程中反应条件的优化
|
摘要
目前有关污泥厌氧消化的研究大都着眼于污泥酸化过程中各种短链脂肪酸产量的研究,对糖和蛋白质在污泥水解和酸化过程中的变化以及产量优化研究较少。本文利用响应面法,探究了污泥的SS、初始ORP以及振荡速率对污泥厌氧发酵过程中的SCOD、糖、蛋白质等有机物产量交互影响,并采用响应面法对实验过程进行了优化。研究结果表明,SCOD受初始污泥的ORP和振荡速率影响较大,与污泥的SS关系不大,并且在SS 8.0g/L、初始ORP 0mV、振荡速率60r/min时能取得最大值9 228.5mg/L;而蛋白质则受SS和初始ORP影响较大,最大值1 215.3mg/L在SS为14.0g/L、初始ORP 0mV、振荡速率105r/min时得到。本实验中,调控各因素对糖的释放影响较小。
Currently most research focus on the different short chain fatty acid production in the process of sludge anaerobic digestion.Fewer studies about the changes of protein and carbohydrates in the sludge hydrolysis and acidification process were reported.In this study,the interactive effects of sludge suspended solids(SS),initial oxidation reduction potential(ORP)and shaking rate on soluble chemistry oxygen demand(SCOD),soluble protein and carbohydrates were investigated in the WAS fermentation.The condition of experiments was optimized using response surface methodology(RSM).The results indicated that SCOD content was little affected by SS but highly affected by ORP and shaking rate,and the maximum concentration 9228.5mg/L of SCOD was achieved at SS 8.0g/L,initial ORP 0mV and shaking rate 60r/min.The maximum concentration ofproteins was achieved at the condition of SS 14.0g/L,initial ORP 0mV and shaking rate 105r/min,and it was highly influenced by the interaction of SS and initial ORP.In this study,the variations of three factors had a little impacts on the release ofcarbohydrates.
Currently most research focus on the different short chain fatty acid production in the process of sludge anaerobic digestion.Fewer studies about the changes of protein and carbohydrates in the sludge hydrolysis and acidification process were reported.In this study,the interactive effects of sludge suspended solids(SS),initial oxidation reduction potential(ORP)and shaking rate on soluble chemistry oxygen demand(SCOD),soluble protein and carbohydrates were investigated in the WAS fermentation.The condition of experiments was optimized using response surface methodology(RSM).The results indicated that SCOD content was little affected by SS but highly affected by ORP and shaking rate,and the maximum concentration 9228.5mg/L of SCOD was achieved at SS 8.0g/L,initial ORP 0mV and shaking rate 60r/min.The maximum concentration ofproteins was achieved at the condition of SS 14.0g/L,initial ORP 0mV and shaking rate 105r/min,and it was highly influenced by the interaction of SS and initial ORP.In this study,the variations of three factors had a little impacts on the release ofcarbohydrates.
引文
[1]范轶,王麒,陈军.微孔塔式曝气用于石化废水处理的研究[J].环境工程,2000,18(6):9-12.Fan Y,Wang Q,Chen J.The study ofmicroporous aeration tower usedin the petrochemical wastewater treatment[J].Environmental Engineering,2000,18(6):9-12.
[2]苏高强,王淑莹,郑冰玉,等.温度和污泥浓度对碱性条件下剩余污泥水解酸化的影响[J].环境工程学报,2013,7(4):1231-1236.Su G Q,Wang S Y,Zheng B Y,et al.Effects of temperature and sludge concentration on hydrolysis and acidification of waste activated sludge under alkaline condition[J].Chinese Journal of Environmental Engineering,2013,7(4):1231-1236.
[3]Kampas P,Parsons S A,Pearce P,et al.An internal carbon source for improving biological nutrient removal[J].Bioresource Technology,2009,100:149-154.
[4]Liu H,Fang H H P.Extraction of extracellular polymeric substances(EPS)of sludge[J].Journal of Biotechnology,2002,95(3):249-256.
[5]Liu X L,Liu H,Chen J H,et al.Enhancement of solubilization and acidification of wasteactivated sludge by pretreatment[J].Waste Management,2008,28(12):2614-2622.
[6]Ma B,Peng Y Z,Wei Y,et al.Free nitrous acid pretreatment of wasted activated sludge to exploit internal carbon source for enhanced denitrification[J].Bioresource Technology,2015,179:20-25.
[7]Chen Y G,Jiang S,Yuan H Y,et al.Hydrolysis and acidification of waste activated sludge at different pHs[J].Water Research,2007,41:683-689.
[8]Karthic P,Joseph S,Arun N,et al.Biohydrogen production using anaerobic mixed bacteria:Process parameters optimization studies[J].Renewable and Sustainable Energy,2013,5(6):127-137.
[9]Guo L,Zhao J,She Z L,et al.Effect of S-TE(solubilization by thermophilic enzyme)digestion conditions on hydrogen production from waste sludge[J].Bioresource Technology,2012,117:368-372.
[10]李玉强,崔振山,陈军,等.基于响应面模型的6σ稳健设计方法[J].上海交通大学学报,2006,40(2):201-205.Li Y Q,Cui Z S,Chen J,et al.Six sigma robust design methodology based on response surface model[J].Journal of Shanghai Jiaotong University,2006,40(2):368-372.
[11]Bosque S J,Pescarolo R L,Garcia C A,et al.Optimizing analytical methods using sequential response surface methodology:Application to the pararosaniline determination of formaldehyde[J].Fresenius’Journal of Analytical Chemistry,2001,369:715-718.
[12]Mannan S,Fakhru’I R A,Alam M Z.Optimization of process parameters for the bioconversion of activated sludge by Penicilllium corylophilum,using response surface methodology[J].Journal of Environmental Sciences,2007,19(1):23-28.
[13]张晓艳,宁喜斌.响应面法优化溶藻弧菌的培养条件[J].食品工业科技,2012,33(16):221-224.Zhang X Y,Ning X B.Optimization of cultivation conditions of vibrio alginolyticus by response surface methodology[J].Science and Technolog of Food Industry,2012,33(16):221-224.
[14]Muralidhar R V,Chirumamila R R,Marchant R,et al.A response surface approach for the comparison of lipase production by Candida cylindracea using two different carbon sources[J].Biochemical Engineering Journal,2001,9(1):17-23.
[15]Lowry O H,Rosebrough N J,Farr A L,et al.Protein measurement with the Folin phenol reagent[J].The Journal of Biological Chemistry,1951,193(1):265-275.
[16]Herbert D,Philipps P,Strange R.Carbohydrate analysis[J].Methods in Enzymol,1971,5B(2):265-277.
[17]国家环境保护总局.水和废水监测分析方法(第四版)[M].北京:中国环境科学出版社,2006:200-275.State Environmental Protection Administration.The Monitoring and Analysis Methods of Water and Waste Water(Ⅳ)[M].Beijing:China Environmental Science Press,2006:200-275.
[18]任南琪,马放,杨基先.污染控制微生物学[M].黑龙江:哈尔滨工业大学出版社,2004:93-100.Ren N Q,Ma F,Yang J X.Polution Control Microbiology[M].Heilongjiang:Harbin Institute of Technology Press,2004:93-100.
[19]Guo L,Zhang J W,She Z L,et al.Optimization of VFAs and ethanol production with waste sludge using as denitrification carbon source[J].Water Science and Technology,2015,72(8):1348-1357.
[2]苏高强,王淑莹,郑冰玉,等.温度和污泥浓度对碱性条件下剩余污泥水解酸化的影响[J].环境工程学报,2013,7(4):1231-1236.Su G Q,Wang S Y,Zheng B Y,et al.Effects of temperature and sludge concentration on hydrolysis and acidification of waste activated sludge under alkaline condition[J].Chinese Journal of Environmental Engineering,2013,7(4):1231-1236.
[3]Kampas P,Parsons S A,Pearce P,et al.An internal carbon source for improving biological nutrient removal[J].Bioresource Technology,2009,100:149-154.
[4]Liu H,Fang H H P.Extraction of extracellular polymeric substances(EPS)of sludge[J].Journal of Biotechnology,2002,95(3):249-256.
[5]Liu X L,Liu H,Chen J H,et al.Enhancement of solubilization and acidification of wasteactivated sludge by pretreatment[J].Waste Management,2008,28(12):2614-2622.
[6]Ma B,Peng Y Z,Wei Y,et al.Free nitrous acid pretreatment of wasted activated sludge to exploit internal carbon source for enhanced denitrification[J].Bioresource Technology,2015,179:20-25.
[7]Chen Y G,Jiang S,Yuan H Y,et al.Hydrolysis and acidification of waste activated sludge at different pHs[J].Water Research,2007,41:683-689.
[8]Karthic P,Joseph S,Arun N,et al.Biohydrogen production using anaerobic mixed bacteria:Process parameters optimization studies[J].Renewable and Sustainable Energy,2013,5(6):127-137.
[9]Guo L,Zhao J,She Z L,et al.Effect of S-TE(solubilization by thermophilic enzyme)digestion conditions on hydrogen production from waste sludge[J].Bioresource Technology,2012,117:368-372.
[10]李玉强,崔振山,陈军,等.基于响应面模型的6σ稳健设计方法[J].上海交通大学学报,2006,40(2):201-205.Li Y Q,Cui Z S,Chen J,et al.Six sigma robust design methodology based on response surface model[J].Journal of Shanghai Jiaotong University,2006,40(2):368-372.
[11]Bosque S J,Pescarolo R L,Garcia C A,et al.Optimizing analytical methods using sequential response surface methodology:Application to the pararosaniline determination of formaldehyde[J].Fresenius’Journal of Analytical Chemistry,2001,369:715-718.
[12]Mannan S,Fakhru’I R A,Alam M Z.Optimization of process parameters for the bioconversion of activated sludge by Penicilllium corylophilum,using response surface methodology[J].Journal of Environmental Sciences,2007,19(1):23-28.
[13]张晓艳,宁喜斌.响应面法优化溶藻弧菌的培养条件[J].食品工业科技,2012,33(16):221-224.Zhang X Y,Ning X B.Optimization of cultivation conditions of vibrio alginolyticus by response surface methodology[J].Science and Technolog of Food Industry,2012,33(16):221-224.
[14]Muralidhar R V,Chirumamila R R,Marchant R,et al.A response surface approach for the comparison of lipase production by Candida cylindracea using two different carbon sources[J].Biochemical Engineering Journal,2001,9(1):17-23.
[15]Lowry O H,Rosebrough N J,Farr A L,et al.Protein measurement with the Folin phenol reagent[J].The Journal of Biological Chemistry,1951,193(1):265-275.
[16]Herbert D,Philipps P,Strange R.Carbohydrate analysis[J].Methods in Enzymol,1971,5B(2):265-277.
[17]国家环境保护总局.水和废水监测分析方法(第四版)[M].北京:中国环境科学出版社,2006:200-275.State Environmental Protection Administration.The Monitoring and Analysis Methods of Water and Waste Water(Ⅳ)[M].Beijing:China Environmental Science Press,2006:200-275.
[18]任南琪,马放,杨基先.污染控制微生物学[M].黑龙江:哈尔滨工业大学出版社,2004:93-100.Ren N Q,Ma F,Yang J X.Polution Control Microbiology[M].Heilongjiang:Harbin Institute of Technology Press,2004:93-100.
[19]Guo L,Zhang J W,She Z L,et al.Optimization of VFAs and ethanol production with waste sludge using as denitrification carbon source[J].Water Science and Technology,2015,72(8):1348-1357.