人类白细胞抗原DQA1、DQB1基因多态性与弓形虫病感染的关联分析
|
摘要
目的探讨杭州汉族人群人类白细胞抗原(human leukocyte antigen,HLA)DQA1、DQB1基因多态性与弓形虫(Toxoplasma gondii)感染的关联性。方法采用病例-对照配对研究及聚合酶链式反应序列特异性引物(PCR-SSP)分型技术,对43例弓形虫感染者以及43例对照组进行HLA-DQA1、HLA-DQB1等位基因分型,并进行基因频率、疾病发生相对危险率(OR)分析。结果与对照组相比,弓形虫感染组HLA-DQA1*0302、HLA-DQB1*0301基因表现频率均显著增高(Pc<0.05);而HLA-DQB1*0504基因表现频率则显著降低(Pc<0.05)。结论 HLA-DQA1*0302、HLA-DQB1*0301等位基因与杭州地区汉族人群的弓形虫易感性相关;HLA-DQB1*0504基因频率可能与弓形虫感染的抵抗性相关联。
Objective To investigate the association of human leukocytes antigen(HLA)-DQA1, DQB1 with Toxoplasma gondii in Hangzhou. Methods HLA-DQA1 and DQB1 genotyping of 43 cases with Toxoplasma gondii infection and 43 normal controls were performed by polymerase chain reaction-sequence specific primers(PCR-SSP) method. Gene frequency and disease relative risk(OR) analysis were performed. Results The frequency of HLA-DQA1*0302 and HLA-DQB1*0301 alleles were significantly higher in Toxoplasma gondii infective group than those in normal controls(Pc<0.05). However, the frequency of HLA-DQB1*0504 was significantly decreased in Toxoplasma gondii infective group than that in normal controls(Pc<0.05). Conclusion HLA-DQA1*0302 and HLA-DQB1*0301 are associated with susceptibility to Toxoplasma gondii in Hangzhou. HLA-DQB1*0504 may be associated with the resistance of Toxoplasma gondii.
Objective To investigate the association of human leukocytes antigen(HLA)-DQA1, DQB1 with Toxoplasma gondii in Hangzhou. Methods HLA-DQA1 and DQB1 genotyping of 43 cases with Toxoplasma gondii infection and 43 normal controls were performed by polymerase chain reaction-sequence specific primers(PCR-SSP) method. Gene frequency and disease relative risk(OR) analysis were performed. Results The frequency of HLA-DQA1*0302 and HLA-DQB1*0301 alleles were significantly higher in Toxoplasma gondii infective group than those in normal controls(Pc<0.05). However, the frequency of HLA-DQB1*0504 was significantly decreased in Toxoplasma gondii infective group than that in normal controls(Pc<0.05). Conclusion HLA-DQA1*0302 and HLA-DQB1*0301 are associated with susceptibility to Toxoplasma gondii in Hangzhou. HLA-DQB1*0504 may be associated with the resistance of Toxoplasma gondii.
引文
[1] Benham A,Tulp A,Neefjes J.Synthexix and assembly of MHC peptide comlex[J].Immunol Today,1995,16(7):359-363.
[2] Carsten GK Lüder,Christine L,Giraldo-Velasquez M,et al.Toxoplasma gondii inhibits MHC class II expression in neural antigen-presenting cells by down-regulating the class II transactivator CIITA[J].J Neuroimmunol,2003,134(1/2):12-24.
[3] Suzuki Y,Wong SY,Grumet FC,et al.Evidence for genetic regulation of susceptibility to toxoplasmic encephalitis in AIDS patients[J].J Infect Dis,1996,173(1):265-268.
[4] Olerup O,Aldener A,Fogdell A.HLA-DQB1 and -DQA1 typing by PCR amplification with sequence-specific primers (PCR-SSP) in 2 hours[J].Tissue Antigen,1993,41:119-134.
[5] Woo HI,Joo EY,Hong SB,et al.Use of PCR with sequence-specific primers for high-resolution human leukocyte antigen typing of patients with narcolepsy[J].Ann Lab Med,2012,32(1):57-65.
[6] Wang FQ,Semana G,Faucher R,et al.HLA-DR and -DQ genotyping by PCR-SSO in Shanghai Chinese[J].Tissue Antigens,1993,41(5):223-226.
[7] 孙逸平,高小江,秋季南.中国人南北群体的HLA Class II基因的变异性[J].中国免疫学杂志,1992,8(5):283-286.
[8] 张修武,郭实士.应用PCR/SSO方法分析湖南籍汉族人HLA-DQ位点DNA的多态性[J].中国免疫学杂志,1993,9(1):18-21.
[9] Gao X,Sun Y,An J,et al.DNA typing for HLA-DR,-DQ and -DP alleles in a Chinese population using the PCR and oligonuceo tide probes[J].Tissue Antigens,1991,38(2):24-30.
[10] 于桂兰,孙逸平,徐林敏.沈阳汉族人群HLA-DR、DQ、DP的DNA分型研究[J].中国免疫学杂志,1995,11(3):142-145.
[11] 张咸宁,帕孜来提,邵红光.用PCR-RFLP方法研究新疆地区汉族HLA-DQA1和-DQB1基因多态性[J].中国免疫学杂志,1996,12(2):89-92.
[12] 陆建荣,潘星华,褚仁远.用PCR-RFLP方法研究江浙沪籍汉族人HLA-DQA1基因多态性[J].中国免疫学杂志,1994,10(6):367-368.
[13] 张卫华,沈福民,孟炜,等.HLA-DQA1、DQB1、DRB102,07,09等位基因及单倍型在华东地区汉族人群的分布[J].中国免疫学杂志,1998,14(2):132-136.
[14] 欧阳琳,李革,贾庆良.重庆部分汉族人群HLA-DQ与弓形虫易感性研究[J].第三军医大学学报,2006,28(14):1511-1513.
[15] Suzuki Y.Host resistance in the brain against Toxoplasma gondii[J].J Infect Dis,2002,185(Suppl 1):S58-S65.
[16] 全湛柔,邓志辉,周丹,等.中国南方汉族造血干细胞捐献者HLA-A、-B、-C、-DRB1及-DQB1高分辨等位基因多态性分析[J].国际输血及血液学杂志,2018,41(6):497-505.
[2] Carsten GK Lüder,Christine L,Giraldo-Velasquez M,et al.Toxoplasma gondii inhibits MHC class II expression in neural antigen-presenting cells by down-regulating the class II transactivator CIITA[J].J Neuroimmunol,2003,134(1/2):12-24.
[3] Suzuki Y,Wong SY,Grumet FC,et al.Evidence for genetic regulation of susceptibility to toxoplasmic encephalitis in AIDS patients[J].J Infect Dis,1996,173(1):265-268.
[4] Olerup O,Aldener A,Fogdell A.HLA-DQB1 and -DQA1 typing by PCR amplification with sequence-specific primers (PCR-SSP) in 2 hours[J].Tissue Antigen,1993,41:119-134.
[5] Woo HI,Joo EY,Hong SB,et al.Use of PCR with sequence-specific primers for high-resolution human leukocyte antigen typing of patients with narcolepsy[J].Ann Lab Med,2012,32(1):57-65.
[6] Wang FQ,Semana G,Faucher R,et al.HLA-DR and -DQ genotyping by PCR-SSO in Shanghai Chinese[J].Tissue Antigens,1993,41(5):223-226.
[7] 孙逸平,高小江,秋季南.中国人南北群体的HLA Class II基因的变异性[J].中国免疫学杂志,1992,8(5):283-286.
[8] 张修武,郭实士.应用PCR/SSO方法分析湖南籍汉族人HLA-DQ位点DNA的多态性[J].中国免疫学杂志,1993,9(1):18-21.
[9] Gao X,Sun Y,An J,et al.DNA typing for HLA-DR,-DQ and -DP alleles in a Chinese population using the PCR and oligonuceo tide probes[J].Tissue Antigens,1991,38(2):24-30.
[10] 于桂兰,孙逸平,徐林敏.沈阳汉族人群HLA-DR、DQ、DP的DNA分型研究[J].中国免疫学杂志,1995,11(3):142-145.
[11] 张咸宁,帕孜来提,邵红光.用PCR-RFLP方法研究新疆地区汉族HLA-DQA1和-DQB1基因多态性[J].中国免疫学杂志,1996,12(2):89-92.
[12] 陆建荣,潘星华,褚仁远.用PCR-RFLP方法研究江浙沪籍汉族人HLA-DQA1基因多态性[J].中国免疫学杂志,1994,10(6):367-368.
[13] 张卫华,沈福民,孟炜,等.HLA-DQA1、DQB1、DRB102,07,09等位基因及单倍型在华东地区汉族人群的分布[J].中国免疫学杂志,1998,14(2):132-136.
[14] 欧阳琳,李革,贾庆良.重庆部分汉族人群HLA-DQ与弓形虫易感性研究[J].第三军医大学学报,2006,28(14):1511-1513.
[15] Suzuki Y.Host resistance in the brain against Toxoplasma gondii[J].J Infect Dis,2002,185(Suppl 1):S58-S65.
[16] 全湛柔,邓志辉,周丹,等.中国南方汉族造血干细胞捐献者HLA-A、-B、-C、-DRB1及-DQB1高分辨等位基因多态性分析[J].国际输血及血液学杂志,2018,41(6):497-505.