DNA甲基化抑制剂对水稻幼苗生长及耐盐性的影响
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摘要
DNA甲基化抑制剂能够通过改变植物基因组的DNA甲基化水平从而对植物的开花、生长发育和抗逆性产生影响。以耐盐和盐敏感水稻品种为材料,分别在水稻萌发期和幼苗期用DNA甲基化抑制剂进行处理,并对处理后的幼苗进行盐胁迫。结果表明:萌发期抑制剂处理导致种子萌发率显著降低,幼苗出现矮化、成簇、死亡等症状,株高、根长和干重也受到了显著的抑制;同时,DNA甲基化抑制剂处理可以降低盐胁迫时地上部的钠离子浓度、提高抗坏血酸过氧化物酶(APX)的活性。上述结果表明DNA甲基化抑制剂通过改变基因组DNA的甲基化水平来影响水稻的生长发育,并通过影响离子吸收和抗氧化酶类的活性来影响水稻的耐盐性。
DNA methylation inhibitors can affect plant flowering,growth and stress responses by altering the genome DNA methylation level. In this study,salt-tolerant and salt-sensitive rice cultivars were treated with DNA methylation inhibitor at germination and seedling stages,respectively. And salt stress was applied to the treated seedlings. The results showed that the DNA methylation inhibitors treatment at germination stage resulted in a significant decrease in germination rate,and the seedlings,which showed short stature,clusters,death and other traits,and plant height,root length and dry weight were also significantly inhibited. Meanwhile,DNA methylation inhibitor could reduce the sodium ion concentration in the shoots under salt stress and increase the activity of ascorbate peroxidase( APX).Above results showed DNA methylation inhibitor affected rice growth and development and the salt tolerance in rice by changing the methylation level of genomic DNA to affect potassium and sodium ion concentrations and antioxidant enzyme activities.
DNA methylation inhibitors can affect plant flowering,growth and stress responses by altering the genome DNA methylation level. In this study,salt-tolerant and salt-sensitive rice cultivars were treated with DNA methylation inhibitor at germination and seedling stages,respectively. And salt stress was applied to the treated seedlings. The results showed that the DNA methylation inhibitors treatment at germination stage resulted in a significant decrease in germination rate,and the seedlings,which showed short stature,clusters,death and other traits,and plant height,root length and dry weight were also significantly inhibited. Meanwhile,DNA methylation inhibitor could reduce the sodium ion concentration in the shoots under salt stress and increase the activity of ascorbate peroxidase( APX).Above results showed DNA methylation inhibitor affected rice growth and development and the salt tolerance in rice by changing the methylation level of genomic DNA to affect potassium and sodium ion concentrations and antioxidant enzyme activities.
引文
[1]黄菲,李雪梅,王文生,等.DNA甲基化在植物抗逆反应中的研究进展及其育种应用[J].中国农业科技导报,2013,(6):83-91.Huang F,Li X M,Wang W S,et al..Research progress of DNA methylation in stress response and breeding in plant[J].J.Agric.Sci.Technol.,2013,(6):83-91.
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[19]陈芳.5-氮杂胞苷对小麦生长发育及DNA甲基化的影响[D].河南开封:河南大学,硕士学位论文,2009.Chen F.Effect of 5-aza C on the development and DNAmethylation of wheat(Triticum aestivum L.)[D].Henan Kaifeng:Henan University,Master Dissertation,2009.
[20]周德龙.5-氮胞苷处理玉米种子对基因组DNA甲基化的影响[D].长春:吉林农业大学,硕士学位论文,2016.Zhou D L.Effect of 5-aza C treatment seeds and genomic DNAmethylation of maize[D].Changchun:Jiling Agricultural University,Master Dissertation,2016.
[21]吴玉香.5-aza C对西洋杜鹃花期及生理指标的影响[D].福州:福建农林大学,硕士学位论文,2017.Wu Y X.Effect of 5-aza C on the flowering and physiological indexes of Rhododendron hybridum[D].Fuzhou:Fujian Agriculture and Forestry University,Master Dissertation,2017.
[2]黄菲.水稻盐胁迫DNA甲基化及MYB转录因子表达谱分析[D].沈阳:沈阳师范大学,硕士学位论文,2013.Huang F.Profiling of DNA methylation and gene expression analysis of MYB transcription factor induced by salinity in rice[D].Shenyang:Shenyang Normal University,Master Dissertation,2013.
[3]Wang W,Zhao X,Pan Y,et al..DNA methylation changes detected by methylation-sensitive amplified polymorphism in two contrasting rice genotypes under salt stress[J].J.Genet.Genomics,2011,38(9):419-424.
[4]Wang W,Huang F,Qin Q,et al..Comparative analysis of DNA methylation changes in two rice genotypes under salt stress and subsequent recovery[J].Biochem.Biophys.Res.Commun.,2015,465(4):790-796.
[5]Wang W,Qin Q,Sun F,et al..Genome-wide differences in DNA methylation changes in two contrasting rice genotypes in response to drought conditions[J].Front.Plant Sci.,2016,7:1675.
[6]钟兰,王建波.DNA超甲基化在小麦耐盐胁迫中的作用[J].植物科学学报,2007,25(1):102-104.
[7]李雪林,林忠旭,聂以春,等.盐胁迫下棉花基因组DNA表观遗传变化的MSAP分析[J].作物学报,2009,35(4):588-596.Li X L,Lin Z X,Nie Y C,et al..MSAP analysis of epigenetic changes in cotton(Gossypium hirsutum L.)under salt stress[J].Acta Agron.Sin.,2009,35(4):588-596.
[8]Karon M,Sieger L,Leimbrok S,et al..5-Azacytidine:A new active agent for the treatment of acute leukemia[J].Blood,1973,42(3):359.
[9]Bhagwat A S,Roberts R J.Genetic analysis of the 5-azacytidine sensitivity of Escherichia coli K-12[J].J.Bacteriol.,1987,169(4):1537-1546.
[10]Castilho A,Neves N,Rufinicastiglione M,et al..5-methylcytosine distribution and genome organization in triticale before and after treatment with 5-azacytidine[J].J.Cell Sci.,1999,112:4397.
[11]King G J.Morphological development in Brassica oleracea is modulated by in vivo treatment with 5-azacytidine[J].J.Hortic.Sci.Biotech.,1995,70(2):333-342.
[12]Burn J E,Bagnall D J,Metzger J D,et al..DNA methylation,vernalization,and the initiation of flowering[J].Proc.Natl.Aca.Sci.USA,1993,90(1):287-291.
[13]Fieldes M A,Schaeffer S M,Krech M J,et al..DNAhypomethylation in 5-azacytidine-induced early-flowering lines of flax[J].Theor.Appl.Genet.,2005,111(1):136.
[14]Belchev I,Tchorbadjieva M,Pantchev I.Effect of 5-azacytidine on callus induction and plant regeneration potential in anther culture of wheat(Triticum aestivum L.)[J].Bulgarian J.Plant Physiol.,2004,1996:1-2.
[15]Romani R J.DNA methylation levels and ethylene production in senescent,suspension-cultured pear fruit cells:Implications for epigenetic control[J]?Physiol.Plantarum.,1998,103(4):534-540.
[16]Zhong L,Xu Y H,Wang J B.The effect of 5-azacytidine on wheat seedlings responses to Na Cl stress[J].Biol.Plantarum.,2010,54(4):753-756.
[17]王文生.水稻盐胁迫全基因组表达谱及抗逆DNA甲基化时空变化分析[D].北京:中国农业科学院,博士学位论文,2011.Wang W S.Genom-wide gene expression profiling of salinity responsiveness and spatial and temporal profiling of DNAmethylation induced by drought and salinity in rice[D].Beijing:Chinese Academy of Agricultural Sciences,Doctor Dissertation,2011.
[18]聂丽娟,王子成.DNA甲基化抑制剂作用机理及其在植物发育生物学研究中的应用[J].核农学报,2007,21(4):362-365.Nie L J,Wang Z C.The molecular mechanism and application of DNA methylation inhibitor in the developmental biology of plants[J].J.Nulear Agric.Sci.,2007,21(4):362-365.
[19]陈芳.5-氮杂胞苷对小麦生长发育及DNA甲基化的影响[D].河南开封:河南大学,硕士学位论文,2009.Chen F.Effect of 5-aza C on the development and DNAmethylation of wheat(Triticum aestivum L.)[D].Henan Kaifeng:Henan University,Master Dissertation,2009.
[20]周德龙.5-氮胞苷处理玉米种子对基因组DNA甲基化的影响[D].长春:吉林农业大学,硕士学位论文,2016.Zhou D L.Effect of 5-aza C treatment seeds and genomic DNAmethylation of maize[D].Changchun:Jiling Agricultural University,Master Dissertation,2016.
[21]吴玉香.5-aza C对西洋杜鹃花期及生理指标的影响[D].福州:福建农林大学,硕士学位论文,2017.Wu Y X.Effect of 5-aza C on the flowering and physiological indexes of Rhododendron hybridum[D].Fuzhou:Fujian Agriculture and Forestry University,Master Dissertation,2017.