基于促斑马鱼血管生成的不同产地巴戟天比较分析
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摘要
目的:通过研究不同产地巴戟天提取物对斑马鱼破损节间血管生长的影响,探讨不同产地巴戟天的地域性差异。方法:建立PTK787诱导的节间血管损伤斑马鱼模型,研究来自广东、广西和福建等12个不同产地巴戟天提取物对斑马鱼节间血管生长的影响,检测给药组斑马鱼节间血管生成数、生成长度,计算血管相对生成率,并在主成分分析的基础上以血管相对生成率为指标,采用SPSS 17.0统计软件对实验数据进行聚类分析。结果:巴戟天提取物对斑马鱼破损节间血管的生长具有修复促进作用,且广东高要、广东郁南等地巴戟天提取物促斑马鱼血管生长效果明显优于其他产区,节间血管生成数平均达到9条,生成长度平均在448~504μm,相对生成率达7.9%~8.86%。聚类分析结果显示,12个不同产地巴戟天被聚为四大类,广东德庆与广东高要,广西苍梧与广西岑溪,广东郁南与广西苍梧等地理位置相近的产地均能较好的聚到一起。结论:不同产地巴戟天在促血管生成作用上存在明显的地域性差异,相关研究为巴戟天道地药材的质量评价提供基础数据。
Objective:To study the effect of Morindae Officinalis Radixe extract from different origins on the vascular growth of damaged internodes of zebra fish,so as to discuss regional differences of Morindae Officinalis Radix from different origins.Method:A zebra fish model of internode vascular injury induced by PTK787 was established to study the effect of Morindae Officinalis Radix extract from Guangdong,Guangxi and Fujian on the growth of internode vascular in zebra fish,and detect the number of internode angiogenesis in zebra fish treated with PTK787.On the basis of principal component analysis,relative angiogenesis rate was taken as the index,and the experimental data was analyzed by SPSS 17.0 statistical software.Result:The extract of Morindae Officinalis Radix had a repairing effect on the growth of damaged internode blood vessels of zebra fish,and the extracts of Morindae Officinalis Radix from Guangdong Gaoyao,and Guangdong Yunan,were better than those from other producing areas.The average number of internode angiogenesis was 9,the average length of angiogenesis was 448-504 μm,and the relative rate of angiogenesis was between 7.9% and 8.86%.The results of cluster analysis showed that Morindae Officinalis Radix of 12 different origins were classified into four categories,Guangdong Deqing,and Guangdong Gaoyao,Guangxi Cangwu and Guangxi Cenxi,and Guangdong Yunan,and Guangxi Cangwu could be better clustered.Conclusion:There are obvious regional differences in the pro-angiogenic effect of Morindae Officinalis Radix from different origins.Relevant studies provide basic data for the quality assessment of Morindae Officinalis Radix medicinal materials.
Objective:To study the effect of Morindae Officinalis Radixe extract from different origins on the vascular growth of damaged internodes of zebra fish,so as to discuss regional differences of Morindae Officinalis Radix from different origins.Method:A zebra fish model of internode vascular injury induced by PTK787 was established to study the effect of Morindae Officinalis Radix extract from Guangdong,Guangxi and Fujian on the growth of internode vascular in zebra fish,and detect the number of internode angiogenesis in zebra fish treated with PTK787.On the basis of principal component analysis,relative angiogenesis rate was taken as the index,and the experimental data was analyzed by SPSS 17.0 statistical software.Result:The extract of Morindae Officinalis Radix had a repairing effect on the growth of damaged internode blood vessels of zebra fish,and the extracts of Morindae Officinalis Radix from Guangdong Gaoyao,and Guangdong Yunan,were better than those from other producing areas.The average number of internode angiogenesis was 9,the average length of angiogenesis was 448-504 μm,and the relative rate of angiogenesis was between 7.9% and 8.86%.The results of cluster analysis showed that Morindae Officinalis Radix of 12 different origins were classified into four categories,Guangdong Deqing,and Guangdong Gaoyao,Guangxi Cangwu and Guangxi Cenxi,and Guangdong Yunan,and Guangxi Cangwu could be better clustered.Conclusion:There are obvious regional differences in the pro-angiogenic effect of Morindae Officinalis Radix from different origins.Relevant studies provide basic data for the quality assessment of Morindae Officinalis Radix medicinal materials.
引文
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[2]国家药典委员会.中华人民共和国药典.一部[M].北京:中国医药科技出版社,2015:81-82.
[3]史辑,刘梓晗,王玲,等.HPLC测定不同产地巴戟天中5种茜草素型蒽醌的含量[J].中药材,2015,38(2):245-248.
[4]章润菁,李倩,屈敏红,等.不同产地、生长年限和种质的巴戟天药材寡糖含量分析[J].中国药学杂志,2016,51(4):315-320.
[5]刘颖嘉,黄宇,荣俊冬,等.巴戟天遗传多样性的ISSR分析[J].福建林学院学报,2011,31(3):203-206.
[6]黄琼林,郑夏生,蔡春.基于ITS2条形码的南药巴戟天真伪鉴别[J].热带作物学报,2014,35(8):1571-1576.
[7]丁平,刘瑾,邱金英,等.基于核糖体r DNAITS序列变异探讨巴戟天道地性[J].药学学报,2012,47(4):535-540.
[8]刘洋洋,冯剑,陈德力,等.南药巴戟天本草考证[J].生物资源,2017,39(1):1-9.
[9]苏现明,王洪庆,陈若芸,等.巴戟天属植物化学成分及药理活性研究进展[J].中药材,2017,40(4):986-991.
[10]赖满香,阮志燕,许意平.补肾中药巴戟天药理作用研究进展[J].亚太传统医药,2017,13(1):63-64.
[11]田丽莉,朱国福.斑马鱼在中药研究中的应用[J].中国中药杂志,2015,40(5):822-827.
[12]赵崇军,田敬欢,王金凤,等.斑马鱼在中药研究中的应用进展[J].中草药,2015,46(17):2635-2648.
[13]何秋霞,刘可春,楚杰,等.斑马鱼作为模式生物在心血管疾病研究中的应用[J].生命的化学,2009,29(5):721-726.
[14]吴峰,王国坤,安丽娜,等.丹参多酚酸盐对斑马鱼胚胎血管新生的影响及分子机制初步研究[J].现代生物医学进展,2012,12(36):7005-7007,7085.
[15]冯国清,杨景柯,于爽,等.巴戟天寡糖促鸡胚绒毛尿囊膜及大鼠心肌梗死模型血管生成的研究[J].中国药理学通讯,2007,24(3):26.
[16]宁青,韦英杰,王丹丹,等.斑马鱼药物代谢模型的适用性研究进展[J].中草药,2015,46(8):1231-1236.
[17]陈志亮.斑马鱼在药物筛选中的应用[J].中国中药杂志,2015,40(7):1235-1239.
[18]张勇,郭胜亚,邓中平,等.斑马鱼在中药毒性靶器官研究中的进展[J].中国新药杂志,2016,25(12):1343-1347.
[19]张楷承,曹雨诞,姚芳,等.京大戟醋制前后对斑马鱼胚胎的急性毒性研究[J].中国中药杂志,2017,42(12):2291-2297.
[20]田丽莉,董建勇,黄长江.莪术醇对斑马鱼胚胎发育的影响[J].温州医学院学报,2010,40(6):557-559,563.