基于荧光光谱特性的水热条件对农田黑土富里酸结构的影响研究
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摘要
为探讨水热条件对农田黑土富里酸(FA)腐殖化程度的影响,选取黑龙江省不同纬度带黑土区域北安、海伦、宾县、双城的农田土壤,对其腐殖物质进行分组测定,且分析了富里酸的荧光光谱特性。结果表明:从北向南,随着水热分布强度增加,农田黑土腐殖酸含量依次降低,北安为9.90g·kg~(-1)、海伦为8.71g·kg~(-1)、宾县为5.48g·kg~(-1)、双城为4.70g·kg~(-1),胡富比显著降低,分别为1.46,1.42,0.80和0.74;不同区域富里酸三维荧光图谱差异值分析表明,与北安相比,其它区域Peak A和Peak C荧光强度均明显减少,区域积分(FRI)方法表明,从北向南,可见光区类富里酸物质含量显著下降;平行因子分析(PARAFAC)表明,各区域土壤FA可以分为相对分子质量较小、结构简单的C1及分子结构相对复杂、缩合度较高的C2两个组分,随着水热梯度增加,C1组分明显增加,而C2组分则呈相反趋势,C1组分的增加主要来源于C2组分降解的中间产物,且较高比例的C1组分促进了FA在土壤中分解;二维同步扫描图谱表明,随着纬度降低,FA结构趋于简单化,黑土中FA分解途径是组分中分子量较大、结构复杂的物质优先裂解,形成结构相对简单的、缩合度较高的小分子化合物,然后进一步彻底分解。综上,北安、海伦、宾县、双城农田黑土的FA浓度及腐殖化程度呈下降趋势;水热梯度增加,不利于土壤中FA积累,加速了土壤FA流失。
In order to understand the influence of hydrothermal conditions to the humification degree of fulvic acid in field blacksoil,four samples Beian,Hailun,Binxian and Shuangcheng which were taken from four typical black soil regions in Heilongjiang,China,were selected for this study.In this study,the fulvic acid substances in these samples were extracted,and characterized their fluorescence properties.The results showed that,from north to south,as the increase of hydrothermal distribution,the concentration of fulvic acid substances,in Beian,Hailun,Binxian and Shuangcheng,presented a decrease trend.And the amount of the decrease were 9.90,8.71,5.48 and 4.70 g·kg~(-1),respectively.Humic acid and fulvic acid ratio is on the decline were 1.46,1.42,0.80 and 0.74 in turn,Higher proportion of fulvic acid leading to Humus structure tends to be more simple.The results of excitation-emission matrix regional integration analysis further confirmed that the intensity of fluorescence peak A and peak C in Beian presented the highest values and intensity of fluorescence peakA and peak C in Shuangcheng presented the lowest values in these four study regions.Compared with Beian,Hailun and Binxian study regional intensity of fluorescence Peak C was lower,and Regional integration(FRI)method showed that,from north to south,as the increase of hydrothermal distribution,visible light area like fulvic acid material significantly reduced;Parallel factor analysis(PARAFAC)results showed that the fulvic acid substances in these four study regions could be divided into two components.Component C1 was characterized as simple structure and low molecular weights substances,and Component C2 was regarded as complex structure and high molecular weights substances.With the increase of hydrothermal distribution,Component C1 was on the rise and Component C2 presented a decrease trend.The increase of Component C1 mainly came from the degradation of the intermediate of Component C2,and a higher proportion of Component C1 promoted the decomposition of FA in the soil.Two-dimensional synchronous scanning map showed that the lower latitude,the simpler structure of humic presented.FA in the black-soil,larger molecular weight and structure of complex material priority cracked form a simple structure relatively,high condensation degree of small molecular compound,and then decomposed completely.Based on the results above,a conclusion could be concluded,that hydrothermal conditions have disadvantage for accumulation of FA substances in black-soil.
In order to understand the influence of hydrothermal conditions to the humification degree of fulvic acid in field blacksoil,four samples Beian,Hailun,Binxian and Shuangcheng which were taken from four typical black soil regions in Heilongjiang,China,were selected for this study.In this study,the fulvic acid substances in these samples were extracted,and characterized their fluorescence properties.The results showed that,from north to south,as the increase of hydrothermal distribution,the concentration of fulvic acid substances,in Beian,Hailun,Binxian and Shuangcheng,presented a decrease trend.And the amount of the decrease were 9.90,8.71,5.48 and 4.70 g·kg~(-1),respectively.Humic acid and fulvic acid ratio is on the decline were 1.46,1.42,0.80 and 0.74 in turn,Higher proportion of fulvic acid leading to Humus structure tends to be more simple.The results of excitation-emission matrix regional integration analysis further confirmed that the intensity of fluorescence peak A and peak C in Beian presented the highest values and intensity of fluorescence peakA and peak C in Shuangcheng presented the lowest values in these four study regions.Compared with Beian,Hailun and Binxian study regional intensity of fluorescence Peak C was lower,and Regional integration(FRI)method showed that,from north to south,as the increase of hydrothermal distribution,visible light area like fulvic acid material significantly reduced;Parallel factor analysis(PARAFAC)results showed that the fulvic acid substances in these four study regions could be divided into two components.Component C1 was characterized as simple structure and low molecular weights substances,and Component C2 was regarded as complex structure and high molecular weights substances.With the increase of hydrothermal distribution,Component C1 was on the rise and Component C2 presented a decrease trend.The increase of Component C1 mainly came from the degradation of the intermediate of Component C2,and a higher proportion of Component C1 promoted the decomposition of FA in the soil.Two-dimensional synchronous scanning map showed that the lower latitude,the simpler structure of humic presented.FA in the black-soil,larger molecular weight and structure of complex material priority cracked form a simple structure relatively,high condensation degree of small molecular compound,and then decomposed completely.Based on the results above,a conclusion could be concluded,that hydrothermal conditions have disadvantage for accumulation of FA substances in black-soil.
引文
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[3]HAO Xiao-di,ZHOU Peng,CAO Ya-li(郝晓地,周鹏,曹亚莉).Chinese Journal of Environmental Engineering(环境工程学报),2017,(1):1.
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[5]Wei Zimin,Wang Xueqin,Zhao Xinyu,et al.International Biodeterioration&Biodegradation,2016,113:187.
[6]ZHAO Yue,HE Xiao-song,XI Bei-dou(赵越,何小松,席北斗).Spectroscopy and Spectral Analysis(光谱学与光谱分析),2010,30(6):1555.
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[8]HU Hui-rong,MA Huan-cheng,LUO Cheng-de(胡慧蓉,马焕成,罗承德).Chinese Journal of Soil Science(土壤通报),2010,(4):1018.
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