Lnc00673蛋白在肺腺癌组织中的表达及意义
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摘要
目的研究Lnc00673在人肺腺癌组织中的表达,探讨该基因对于肺腺癌的临床意义。方法收集84例肺腺癌组织和30例肺癌旁肺组织,采用免疫组化法检测Lnc00673蛋白的表达,并分析其与肺腺癌临床病理特征之间的关系。结果 Lnc00673蛋白的表达在肺腺癌组织中显著高于癌旁组织(P<0.000 1)。Ⅲ、IV期肺腺癌中Lnc00673阳性表达率显著高于Ⅰ、Ⅱ期肺癌(P<0.01);Lnc00673的表达在有淋巴结转移者明显高于无淋巴结转移者(P<0.05)。结论 Lnc00673在肺腺癌组织中表达增高,并与肺癌的分期、淋巴结转移相关;Lnc00673可作为评估肺癌患者病变范围和预后的标志物。
Objective To investigate the expression of Lnc00673 protein in human lung adenocarcinoma and its clinical significance. Methods The protein expression of Lnc00673 was detected by immunohistochemistry in 84 specimens of lung adenocarcinoma, and 30 specimens of pericancerous tissues. The correlation between Lnc00673 expression and clinicopathological features of lung cancer was analyzed. Results The expression of Lnc00673 protein in lung adenocarcinoma was significantly higher than that in adjacent tissue(P<0.000 1). The protein expression levels of CPNE3 in Stage Ⅲ and Ⅳ lung adenocarcinoma were significantly higher than those in Stage Ⅰ and Ⅱ(P<0.01). The expression of Lnc00673 was significantly higher in those with lymph node metastasis(P<0.05). Conclusion The expression of Lnc00673 protein is significantly increased in lung adenocarcinoma, and is correlated with TNM staging and lymph node metastasis. Which indicates that Lnc00673 can be used as a marker for evaluating the extent and prognosis of lung cancer.
Objective To investigate the expression of Lnc00673 protein in human lung adenocarcinoma and its clinical significance. Methods The protein expression of Lnc00673 was detected by immunohistochemistry in 84 specimens of lung adenocarcinoma, and 30 specimens of pericancerous tissues. The correlation between Lnc00673 expression and clinicopathological features of lung cancer was analyzed. Results The expression of Lnc00673 protein in lung adenocarcinoma was significantly higher than that in adjacent tissue(P<0.000 1). The protein expression levels of CPNE3 in Stage Ⅲ and Ⅳ lung adenocarcinoma were significantly higher than those in Stage Ⅰ and Ⅱ(P<0.01). The expression of Lnc00673 was significantly higher in those with lymph node metastasis(P<0.05). Conclusion The expression of Lnc00673 protein is significantly increased in lung adenocarcinoma, and is correlated with TNM staging and lymph node metastasis. Which indicates that Lnc00673 can be used as a marker for evaluating the extent and prognosis of lung cancer.
引文
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[3] Minami S,Oqata Y,Ihara S,et al.Neutrophil-to-Lymphocyte Ratio Predicts Overall Survival of Advanced Non-Small Cell Lung Cancer Harboring Mutant Epidermal Growth Factor Receptor[J].World Oncol,2017,8(6):180-187.
[4] Morris KV.Long antisense non-coding RNAs function to direct epigenetic complexes that regulate transcription in human cells[J].Epigenetics,2009,4(5):296-301.
[5] Villegas VE,Zaphiropoulos PG.Neighboring gene regulation by antisense long Non-Coding RNAs[J].Int J Mol Sci,2015,16(2):3251-3266.
[6] Mattick JS.RNA regulation:a new genetics?[J].Nat Rev Genet,2004,5(4):316-323.
[7] Chen G,Sun W,Hua X,et al.Long non-coding RNA FOXD2-AS1 aggravates nasopharyngeal carcinoma carcinogenesis by modulating miR-363-5p/S100A1 pathway[J].Gene,2018,645:76-84.
[8] Liu B,Pan CF,Ma T,et al.Long non-coding RNA AK001796 contributes to cisplatin resistance of non-small cell lung cancer[J].Mol Med Rep,2017,16(4):4107-4112.
[9] Yu C,Chen K,Zheng H,et al.Overexpression of astrocyte elevated gene-1 (AEG-1) is associated with esophageal squamous cell carcinoma (Escc) progression and pathogenesis[J].Carcinogenesis,2009,30(5):894-901.
[10] Cassidy RJ,Zhang X,Switchenko JM,et al.Health care disparities among octogenarians and nonagenarians with stage III lung cancer[J].Cancer,2018,124(4):775-784.
[11] Ettinger DS,Akerley W,Bepler G,et al.Non-small cell lung cancer[J].J Natl Compr Canc Netw,2010,8(7):740-801.
[12] Zong L,Sun Q,Zhang H,et al.Increased expression of circRNA_102231 in lung cancer and its clinical significance[J].Biomed Pharmacother,2018,102:639-644.
[13] Huang JL,Zheng L,Hu YW,et al.Characteristics of long non-coding RNA and its relation to hepatocellular carcinoma[J].Carcinogenesis,2014,35(3):507-514.
[14] Fatica A,Bozzoni I.Long non-coding RNAs:new players in cell differentiation and development[J].Nat Rev Genet,2014,15(1):7-21.
[15] Gibb EA,Brown CJ,Lam WL.The functional role of long non-coding RNA in human carcinomas[J].Mol Cancer,2011,10:38.
[16] Abdul-Rahman U,Gy?rffy B,Adams BD.linc00673 (ERRLR01) is a prognostic indicator of overall survival in breast cancer[J].Transcription,2018,9(1):17-29.
[17] Isaksson HS,Sorbe B,Nilsson TK.Whole genome expression profiling of blood cells in ovarian cancer patients-prognostic impact of the CYP1B1,MTSS1,NCALD,and NOP14[J].Oncotarget,2014,5(12):4040-4049.
[18] Chikaishi Y,Shinohara S,Kuwata T,et al.Complete resection of the primary lesion improves survival of certain patients with stage Ⅳ non-small cell lung cancer[J].J Thorac Dis,2017,9(12):5278-5287.
[19] Gomez DR,Byers LA,Nilsson M,et al.Integrative proteomic and transcriptomic analysis provides evidence for TrkB (NTRK2) as a therapeutic target in combination with tyrosine kinase inhibitors for non-small cell lung cancer[J].Oncotarget,2018,9(18):14268-14284.
[20] Tomita M,Ayabe T,Maeda R,et al.Combination of Advanced Lung Cancer Inflammation Index and C-Reactive Protein is a Prognostic Factor in Patients with Operable Non-Small Cell Lung Cancer[J].World J Oncol,2017,8(6):175-179.
[21] Sun X,Huang T,Cheng F,et al.Monitoring colorectal cancer following surgery using plasma circulating tumor DNA[J].Oncol Lett,2018,15(4):4365-4375.
[22] Tomia M,Ayabe T,Nakamura K.Low Body Mass Index is an Independent Predictive Fator after Surgical Resection in Patient with Non-Small Cell Lung Cancer[J].Asian Pac J Cancer Prev,2017,18(12):3353-3356.
[23] Lu H,Chen B,Qin J,et al.Transformation to small-cell lung cancer following treatment with icotinib in a patient with lung adenocarcinoma[J].Oncol Lett,2018,15(4):5799-5802.
[24] Lu W,Zhang H,Niu Y,et al.Long non-coding RNA linc00673 regulated non-small cell lung cancer proliferation,migration,invasion and epithelial mesenchymal transition by sponging miR-150-5p[J].Mol Cancer,2017,16(1):118.
[25] Shi X,Ma C,Zhu Q,et al.Upregulation of long intergenic noncoding RNA 00673 promotes tumor proliferation via LSD1 interaction and repression of NCALD in non-small-cell lung cancer[J].Oncotarget,2016,7(18):25558-25575.