朱砂根幼苗在不同光照强度下的形态和生理响应
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摘要
通过人工遮荫模拟不同生境光强(100%、52%、33%、15%和6%的相对光照)的方法,对朱砂根(Ardisia crenata Sims)幼苗形态、生物量和生理指标的变化特征进行了分析。研究结果显示,朱砂根单株生物量在52%相对光照处理下显著高于其他光照处理,在15%～52%相对光照处理下分配给叶片的生物量比例高于全光照(100%)和极弱(6%)光照处理,但根冠比不受光强影响,其结构可塑性相对较低。硝酸盐含量随遮荫强度的增加而增大,且在6%相对光照处理下显著增加,硝酸盐还原酶的活性同硝酸盐含量变化规律一致,表明朱砂根主要通过改变叶面积和比叶面积等形态指标,以及调整生物量的分配和光合色素含量来适应不同的光环境。叶绿体超微结构分析结果显示,在15%～52%相对光照处理下,叶绿体数量较多且细胞结构较完整,而100%和6%光照处理下的叶绿体数量明显减少,且细胞结构严重受损发生质壁分离现象。因此,朱砂根适宜生长的光照条件为15%～52%,尤其是33%～52%的相对光照条件更佳。
Changes in the morphology,biomass,and physiology of Ardisia crenata seedlings were studied under different light environments( 100%,52%,33%,15%,and 6% relative light intensities). Results showed that the biomass of individual A. crenata seedlings was significantly higher under 52% light treatment than that under other treatments. The proportion of biomass allocated to leaves under 15%-52% light treatment was also higher than that under 100% and 6% light treatment. The root shoot ratio was not affected by light intensity,indicating that the structural plasticity of A. crenata was relatively low. The concentrations of nitrate increased with the decrease in light intensity and increased significantly under 6% light treatment. The change in nitrate reductase activity with light intensity was consistent with the change in nitrate content. Furthermore,the A. crenata seedlings adapted to different light environments by changing the leaf area and specific leaf area and by regulating thephotosynthetic pigment. Analysis of chloroplast ultrastructure also showed that chloroplast number and cellular structure under 15%-52% light treatment were intact. However,under100% and 6% light treatment,the number of the chloroplasts decreased significantly,the cellular structure was damaged, and plasmolysis occurred. Therefore, the most suitable growth conditions for A. crenata seedlings were under 15%-52% relative light intensities,though the best growth was achieved under 33%-52% relative light treatment.
Changes in the morphology,biomass,and physiology of Ardisia crenata seedlings were studied under different light environments( 100%,52%,33%,15%,and 6% relative light intensities). Results showed that the biomass of individual A. crenata seedlings was significantly higher under 52% light treatment than that under other treatments. The proportion of biomass allocated to leaves under 15%-52% light treatment was also higher than that under 100% and 6% light treatment. The root shoot ratio was not affected by light intensity,indicating that the structural plasticity of A. crenata was relatively low. The concentrations of nitrate increased with the decrease in light intensity and increased significantly under 6% light treatment. The change in nitrate reductase activity with light intensity was consistent with the change in nitrate content. Furthermore,the A. crenata seedlings adapted to different light environments by changing the leaf area and specific leaf area and by regulating thephotosynthetic pigment. Analysis of chloroplast ultrastructure also showed that chloroplast number and cellular structure under 15%-52% light treatment were intact. However,under100% and 6% light treatment,the number of the chloroplasts decreased significantly,the cellular structure was damaged, and plasmolysis occurred. Therefore, the most suitable growth conditions for A. crenata seedlings were under 15%-52% relative light intensities,though the best growth was achieved under 33%-52% relative light treatment.
引文
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