不同来源香港海鸥形菌耐药性分析与相关耐药基因的检测
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摘要
目的研究不同来源香港海鸥形菌(Laribacter hongkongensis,LH)的耐药特性及相关耐药基因的检出情况。方法选取腹泻患者分离及淡水鱼肠道分离的共26株LH,用K-B法进行19种抗菌药物的药敏试验;并采用PCR技术筛查菌株β-内酰胺类、整合子及四环素类耐药基因的携带情况。结果 26株香港海鸥形菌对19种常用抗菌药物中的10种表现出不同程度的耐药现象。LH对青霉素类、第二代及第三代头孢类抗菌药物的耐药率较高,均在42.0%以上,尤其是对青霉素和头孢哌酮的耐药率达100.0%。人来源菌株与鱼来源菌株的耐药率相比较,差异无统计学意义(P>0.05)。26株LH检出ampC基因阳性菌株26株(100.0%),1株(3.8%)携带tetA基因,3株(11.5%)携带CTX-M-9基因,6株(23.1%)携带intⅠ型整合子基因。结论香港海鸥型菌对青霉素类和头孢类药物呈普遍耐药,并常携带CTX-M-9型超广谱β-内酰胺酶(ESBLs)基因和intⅠ型整合子基因。
Objective To study the drug resistance and antibiotic-resistant genes of Laribacter hongkongensis(LH) from different sources. Methods Kerby-Bauer method was used to detect the drug sensitivity of 26 strains of LH from human patients with community-acquired gastroenteritis and freshwater fish. The antibiotic-resistant genes were amplified by PCR. Results The results showed that LH strains had different resistance to 10 kinds of 19 antibacterial drugs. LH strains had high levels resistance to penicillins, 2~(ed) and 3~(rd) cephalosporin, the rates were more than 42.0%. The resistance rates of it to penicillin and cefoperazone were 100.0%. There was no statistical significance on the difference in the drug resistance rate of strains from human patients and freshwater fish(P<0.05). The positive rate of ampC gene was 100.0% in 26 LH strains. 1(3.8%) strain carried tetA gene, 3(11.5%) strains carried CTX-M-9 gene, and 6(23.1%) strains carried class Ⅰ integron gene. Conclusion LH strains is generally resistant to penicillins and cephalosporin, and usually carries CTX-M-9 ESBLs gene and class Ⅰ integron gene.
Objective To study the drug resistance and antibiotic-resistant genes of Laribacter hongkongensis(LH) from different sources. Methods Kerby-Bauer method was used to detect the drug sensitivity of 26 strains of LH from human patients with community-acquired gastroenteritis and freshwater fish. The antibiotic-resistant genes were amplified by PCR. Results The results showed that LH strains had different resistance to 10 kinds of 19 antibacterial drugs. LH strains had high levels resistance to penicillins, 2~(ed) and 3~(rd) cephalosporin, the rates were more than 42.0%. The resistance rates of it to penicillin and cefoperazone were 100.0%. There was no statistical significance on the difference in the drug resistance rate of strains from human patients and freshwater fish(P<0.05). The positive rate of ampC gene was 100.0% in 26 LH strains. 1(3.8%) strain carried tetA gene, 3(11.5%) strains carried CTX-M-9 gene, and 6(23.1%) strains carried class Ⅰ integron gene. Conclusion LH strains is generally resistant to penicillins and cephalosporin, and usually carries CTX-M-9 ESBLs gene and class Ⅰ integron gene.
引文
[1] Yuen K,Woo P,Teng J,et al.Laribacter hongkongensis gen.nov.,sp.nov.,a novel gram-negative bacterium isolated from a cirrhotic patient with bacteremia and empyema[J].J Clin Microbiol,2001,39:4227-4232.
[2] Woo P,Lau S,Teng J,et al.Association of Laribacter hongkongensis in community-acquired gastroenteritis with travel and eating fish:a multicentre case-control study[J].Lancet,2004,363:1941-1947.
[3] Woo P,Lau S,Teng J,et al.Current status and future directions for Laribacter hongkongensis,a novel bacterium associated with gastroenteritis and traveller's diarrhea[J].Curr Opin Infect Dis,2005,18:413-419.
[4] 任淑华,倪晓平,孙建荣,等.不同来源株香港海鸥形菌药敏试验结果分析[J].中国微生态杂志,2007,19(6):537-539.
[5] 宋曼丹,严纪文,朱海明,等.广东省水产品中香港海鸥形菌的污染调查与耐药性检测[J].中国热带医学,2007,7(8):1473-1474.
[6] 朱丽东,肖锋,杨羚,等.香港海鸥型菌对喹诺酮耐药性的检测分析[J].实用医学杂志,2014,30(19):3166-3169.
[7] Ni X,Ren S,Sun J,et al.Laribacter hongkongensis isolated from a patient with community-acquired gastroenteritis in Hangzhou City[J].J Clin Microbiol,2007,45:255-256.
[8] Clinical and Laboratory Standards Institute (CLSI).Performance Standard for Antimicrobial Susceptibility testing Fourteenth Informational Supplement.CLSI Document M100-S17[S].Wayne,USA:Clinical and Laboratory Standards Institute,2007.
[9] Raja K,Ghosh A.Molecular insight of putative pathogenicity markers with ESBL genes and lipopolysaccharide in Laribacter hongkongensis[J].Appl Biochem Biotechnol,2014,174(5):1935-1944.
[10] Teng J,Woo P,Ma S,et al.Ecoepidemiology of Laribacter hongkongensis,a Novel Bacterium Associated with Gastroenteritis,in retail freshwater fish in Hong Kong[J].J Food Microbiol,2007,113(1):62-66.
[11] Lau SK,Woo PC,Fan RY,et al.Isolation of Laribacter hongkongensis,a novel bacterium associated with gastroenteritis,from drinking water reservoirs in Hong Kong[J].J Appl Microbiol,2007,103(3):507-515.
[12] Lau SK,Wong GK,Poon RW,et al.Susceptibility patterns of clinical and fish isolates of Laribacter hongkongensis:comparison of the Etest,disc diffusion and broth microdilution methods[J].J Antimicrob Chemother,2009,63(4):704-708.
[13] 季淑娟,顾怡明,谭文涛,等.中国部分地区大肠埃希菌和肺炎克雷伯菌超广谱β内酰胺酶基因型研究[J].中华检验医学杂志,2004,27(9):590-593.
[14] 罗毓婷,陈定强,杨羚,等.香港海鸥型菌四环素耐药性及其耐药分子机制研究[J].实用医学杂志,2013,29(4):630-632.
[15] 李彦媚,赵喜红,徐泽智,等.新型细菌耐药元件——整合子系统[J].中国抗生素杂志,2012,37(1):1-7.
[16] 赵亚梅,黄震,陈定强,等.香港海鸥型菌耐喹诺酮类抗菌药物机制的研究[J].国际检验医学杂志,2017,38(9):1179-1182.
[17] 陈冰,冯嘉丽,李美霞,等.耐复方新诺明香港海鸥型菌中Ⅰ类整合子、磺胺类耐药基因(sul1、sul2)的检测[J].中国人兽共患病学报,2012,28(3):223-225.
[2] Woo P,Lau S,Teng J,et al.Association of Laribacter hongkongensis in community-acquired gastroenteritis with travel and eating fish:a multicentre case-control study[J].Lancet,2004,363:1941-1947.
[3] Woo P,Lau S,Teng J,et al.Current status and future directions for Laribacter hongkongensis,a novel bacterium associated with gastroenteritis and traveller's diarrhea[J].Curr Opin Infect Dis,2005,18:413-419.
[4] 任淑华,倪晓平,孙建荣,等.不同来源株香港海鸥形菌药敏试验结果分析[J].中国微生态杂志,2007,19(6):537-539.
[5] 宋曼丹,严纪文,朱海明,等.广东省水产品中香港海鸥形菌的污染调查与耐药性检测[J].中国热带医学,2007,7(8):1473-1474.
[6] 朱丽东,肖锋,杨羚,等.香港海鸥型菌对喹诺酮耐药性的检测分析[J].实用医学杂志,2014,30(19):3166-3169.
[7] Ni X,Ren S,Sun J,et al.Laribacter hongkongensis isolated from a patient with community-acquired gastroenteritis in Hangzhou City[J].J Clin Microbiol,2007,45:255-256.
[8] Clinical and Laboratory Standards Institute (CLSI).Performance Standard for Antimicrobial Susceptibility testing Fourteenth Informational Supplement.CLSI Document M100-S17[S].Wayne,USA:Clinical and Laboratory Standards Institute,2007.
[9] Raja K,Ghosh A.Molecular insight of putative pathogenicity markers with ESBL genes and lipopolysaccharide in Laribacter hongkongensis[J].Appl Biochem Biotechnol,2014,174(5):1935-1944.
[10] Teng J,Woo P,Ma S,et al.Ecoepidemiology of Laribacter hongkongensis,a Novel Bacterium Associated with Gastroenteritis,in retail freshwater fish in Hong Kong[J].J Food Microbiol,2007,113(1):62-66.
[11] Lau SK,Woo PC,Fan RY,et al.Isolation of Laribacter hongkongensis,a novel bacterium associated with gastroenteritis,from drinking water reservoirs in Hong Kong[J].J Appl Microbiol,2007,103(3):507-515.
[12] Lau SK,Wong GK,Poon RW,et al.Susceptibility patterns of clinical and fish isolates of Laribacter hongkongensis:comparison of the Etest,disc diffusion and broth microdilution methods[J].J Antimicrob Chemother,2009,63(4):704-708.
[13] 季淑娟,顾怡明,谭文涛,等.中国部分地区大肠埃希菌和肺炎克雷伯菌超广谱β内酰胺酶基因型研究[J].中华检验医学杂志,2004,27(9):590-593.
[14] 罗毓婷,陈定强,杨羚,等.香港海鸥型菌四环素耐药性及其耐药分子机制研究[J].实用医学杂志,2013,29(4):630-632.
[15] 李彦媚,赵喜红,徐泽智,等.新型细菌耐药元件——整合子系统[J].中国抗生素杂志,2012,37(1):1-7.
[16] 赵亚梅,黄震,陈定强,等.香港海鸥型菌耐喹诺酮类抗菌药物机制的研究[J].国际检验医学杂志,2017,38(9):1179-1182.
[17] 陈冰,冯嘉丽,李美霞,等.耐复方新诺明香港海鸥型菌中Ⅰ类整合子、磺胺类耐药基因(sul1、sul2)的检测[J].中国人兽共患病学报,2012,28(3):223-225.