成纤维细胞生长因子FGF11、13、18对小鼠毛囊第一生长周期作用的研究
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摘要
为了探究成纤维生长因子家族(Fibroblast growth factor families,FGFs)中,FGF11、13、18对毛囊的生长发育的作用,本研究以3/5/8/13/16/18/20/23日龄小鼠背部皮肤为试验样本,利用免疫组织化学技术、实时荧光定量PCR和蛋白免疫印迹技术检测FGF11、FGF13、FGF18基因及蛋白在小鼠背部皮肤的分布和表达情况。定位结果显示:FGF11、FGF13、FGF18在皮肤真皮乳头、毛基质、内根鞘、外根鞘和皮脂腺表达,而FGF13在膨大部、基底层和表皮也表达,FGF18也表达在膨大部和表皮。定量结果均显示:FGF11的表达没有一定的规律;FGF13在13日龄表达量最低,从16日龄开始上升;FGF18从3日龄表达量开始降低,16日龄降到最低,18日龄开始上升。提示:FGF11对小鼠毛囊第一生长周期可能没有明显和特定的调节作用;FGF13可能对毛囊的自我更新有一定的作用,诱导毛囊从静止期向再生长期过渡,促进毛囊生长;而FGF18在毛囊生长周期中从静止期向生长期过度有一定的作用,可促使毛囊从静止期进入再生长期。
In order to explore the role of FGF11,13 and 18 in the growth and development of hair follicles,we used 3/5/8/13/16/18/20/23 day-old mice as the experimental animals,and the distribution and expression of FGF11, FGF13 and FGF18 genes and proteins in the dorsal skin of mice. were dectected by IHC-P,RT-PCR and western blot. The results showed that FGF11, 13 and 18 were expressed in the dermal papilla,hair follicle matrix, inner root sheath, outer root sheath and sebaceous gland, while FGF13 was also expressed in the bulge, basal layer and epidermis. FGF18 was also expressed in the bulge and epidermis. The expression data showed that the expression of FGF11 was not the same. FGF13 was the lowest at 13 days of age and increased from 16 days of age. FGF18 was decreased from 3 days of age,to the lowest at 16 days,and then 18 days began to rise at the age of 18 days.The experimental results suggest that: FGF11 has no clear and specific regulation to postnatal mice skin hair follicle growth cycle; FGF13 may have certain effect on hair follicle self-renewal, induce Hair follicles from telogen to anagen stage, and promotes hair follicle growth. FGF18 plays certain role in hair follicle transition from tolegen to anagen,and stimulates the hair follicles into growth phase from quiescence.
In order to explore the role of FGF11,13 and 18 in the growth and development of hair follicles,we used 3/5/8/13/16/18/20/23 day-old mice as the experimental animals,and the distribution and expression of FGF11, FGF13 and FGF18 genes and proteins in the dorsal skin of mice. were dectected by IHC-P,RT-PCR and western blot. The results showed that FGF11, 13 and 18 were expressed in the dermal papilla,hair follicle matrix, inner root sheath, outer root sheath and sebaceous gland, while FGF13 was also expressed in the bulge, basal layer and epidermis. FGF18 was also expressed in the bulge and epidermis. The expression data showed that the expression of FGF11 was not the same. FGF13 was the lowest at 13 days of age and increased from 16 days of age. FGF18 was decreased from 3 days of age,to the lowest at 16 days,and then 18 days began to rise at the age of 18 days.The experimental results suggest that: FGF11 has no clear and specific regulation to postnatal mice skin hair follicle growth cycle; FGF13 may have certain effect on hair follicle self-renewal, induce Hair follicles from telogen to anagen stage, and promotes hair follicle growth. FGF18 plays certain role in hair follicle transition from tolegen to anagen,and stimulates the hair follicles into growth phase from quiescence.
引文
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[2] Zhang X J,Yu J,Huang Y X,et al.Effects of recombinant human endostatin on the expression of vascular endothelial growth factor,tranformina arowth factor-β1 and basic fibroblast growth factor on on hypertrophic scar in rabbit ears[J].Acta Anatomica Sinica,2015,46(01):101-105.
[3] Suzuki S,Ota Y,Ozawa K,et al.Dual-mode regulation of hair growth cycle by two Fgf-5 gene products[J].Journal of investigative dermatology,2000,114(3):456-463.
[4] Du W,Prochazka J,Prochazkova M,et al.Expression of FGFs during early mouse tongue development[J].Gene Expression Patterns,2016,20(2):81-87.
[5] Oshimori N,Fuchs E.Paracrine TGF-β signaling counterbalances BMP-mediated repression in hair follicle stem cell activation[J].Cell stem cell,2012,10(1):63-75.
[6] Mason I J,Fuller-Pace F,Smith R,et al.FGF-7 (keratinocyte growth factor) expression during mouse development suggests roles in myogenesis,forebrain regionalisation and epithelial-mesenchymal interactions[J].Mechanisms of development,1994,45(1):15-30.
[7] Finch P W,Cunha G R,Rubin J S,et al.Pattern of keratinocyte growth factor and keratinocyte growth factor receptor expression during mouse fetal development suggests a role in mediating morphogenetic mesenchymal‐epithelial interactions[J].Developmental dynamics,1995,203(2):223-240.
[8] Xu J,Lawshé A,MacArthur C A,et al.Genomic structure,mapping,activity and expression of fibroblast growth factor 17[J].Mechanisms of development,1999,83(1):165-178.
[9] Liu Z,Xu J,Colvin J S,et al.Coordination of chondrogenesis and osteogenesis by fibroblast growth factor 18[J].Genes & development,2002,16(7):859-869.
[10] Ohbayashi N,Shibayama M,Kurotaki Y,et al.FGF18 is required for normal cell proliferation and differentiation during osteogenesis and chondrogenesis[J].Genes & development,2002,16(7):870-879.
[11] Greco V,Chen T,Rendl M,et al. A two-step mechanism for stem cell activation during hair regeneration[J]. Cell stem cell,2009,4(2):155-169.
[12] Goldfarb M. Fibroblast growth factor homologous factors:evolution,structure,and function[J]. Cytokine&growth factor reviews,2005,16(2):215-220.
[13] Gabrielsson B G,Johansson J M,Jennische E,et al. Depot-Specific Expression of Fibroblast Growth Factors in Human Adipose Tissue[J]. Obesity,2002,10(7):608-616.
[14] Kawano M,Komi-Kuramochi A,Asada M,et al. Comprehensive analysis of FGF and FGFR expression in skin:FGF18 is highly expressed in hair follicles and capable of inducing anagen from telogen stage hair follicles[J]. Journal of Investigative Dermatology,2005,124(5):877-885.
[15] LIVAK K J,SCHMITTGEN T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T))Method[J]. Methods,2001,25(4):402-408.