通过比较基因组杂交研究肝癌中非随机染色体畸变
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摘要
本研究通过使用比较基因组杂交的方法,研究肝癌中的非随机染色体畸变,旨在为肝癌的临床诊断和治疗提供参考资料。本研究收集了我院在2016年9月至2017年9月收治的10例肝癌患者,采集其肝癌标本。使用比较基因组杂交法(CGH)对所有肝癌标本的非随机染色体畸变进行分析。结果表明,所有肝癌标本均出现了非随机染色体畸变,即4q区域、16q区域、8p区域和17p区域的缺失变化。同时在1q区域、8q区域、17q区域以及6p区域出现了扩增变化。本研究初步得出结论,在肝癌出现后,其非随机区域会出现染色体畸变,这些情况的出现可对肝癌进行较好诊断,同时也可指导对肝癌的治疗。
In this study, non-random chromosomal aberrations in hepatocellular carcinoma(HCC) were studied using comparative genomic hybridization to provide references for clinical diagnosis and treatment of liver cancer.In this study, 10 patients with liver cancer admitted to our hospital from September 2016 to September 2017 were collected, and their liver cancer specimens were collected. Comparative genomic hybridization(CGH) was used to analyze the nonrandom chromosomal aberrations in all liver cancer specimens. The results showed that all HCC specimens presented non-random chromosomal aberrations, with missing changes in 4 q region, 16 q region, 8 p region and 17 p region,while the increased changes occurred in regions 1 q, 8 q, 17 q and 6 p. This research preliminarily concluded that chromosomal aberrations may occur in non-random regions after the occurrence of liver cancer,which can be used to make a good diagnosis of liver cancer and guide the treatment of liver cancer.
In this study, non-random chromosomal aberrations in hepatocellular carcinoma(HCC) were studied using comparative genomic hybridization to provide references for clinical diagnosis and treatment of liver cancer.In this study, 10 patients with liver cancer admitted to our hospital from September 2016 to September 2017 were collected, and their liver cancer specimens were collected. Comparative genomic hybridization(CGH) was used to analyze the nonrandom chromosomal aberrations in all liver cancer specimens. The results showed that all HCC specimens presented non-random chromosomal aberrations, with missing changes in 4 q region, 16 q region, 8 p region and 17 p region,while the increased changes occurred in regions 1 q, 8 q, 17 q and 6 p. This research preliminarily concluded that chromosomal aberrations may occur in non-random regions after the occurrence of liver cancer,which can be used to make a good diagnosis of liver cancer and guide the treatment of liver cancer.
引文
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Chen Y.J.,Yeh S.H.,Chen J.T.,Wu C.C.,Hsu M.T.,Tsai S.F.,Chen P.J.,and Lin C.H.,2000,Chromosomal changes and clonality relationship between primary and recurrent hepatocellular carcinoma,Gastroenterology,119(2):431-440
Gebhart E.,2004,Comparative genomic hybridization(CGH):ten years of substantial progress in human solid tumor molecular cytogenetics,Cytogenetic and Genome Research,104(1-4):352-358
He S.Q.,Cong W.M.,Hu L.Y.,Yan J.X.,Dong H.,Chen L.,Min B.J.,and Zhu Z.Z.,2015,Copy number alterations and phenotyp ic genes in chromosome 4 long arm related to recurrence of hepatocellular carcinoma,Zhonghua Shiyan Waike Zazhi(Chinese Journal of Experimental Surgery),32(10):2376-2379(贺松琴,丛文铭,胡柳燕,严静娴,董辉,陈律,闵炳基,朱忠政,2015,4号染色体长臂拷贝数变异及复发表征基因与肝癌复发,中华实验外科杂志,32(10):2376-2379)
Pan Q.,and Ding K.Y.,2015,Droplet digital PCR validates a novel nonsense somatic mutation in MSH2 in hepatic carcinoma,Disan Junyi Daxue Xuebao(Journal of Third Military Medical University),37(23):2368-2372(潘琦,丁克越,2015,微滴数字化PCR检测肝癌基因组MSH2基因的一个新的无义突变,第三军医大学学报,37(23):2368-2372)
Qiu S.,and Zhang H.Y.,2015,Detection of human hepatocarcinoma cell line PLC/PRF/5 genome hepatitis b virus DNA integration with Alu-PCR,Yixue Yanjiu Zazhi(Journal of Me-dical Research),44(4):88-90,41(邱爽,张会英,2015,Alu-PCR检测人肝癌细胞株PLC/PRF/5基因组中乙型肝炎病毒DNA的整合,医学研究杂志,44(4):88-90,41)
Rao U.N.,Gollin S.M.,Beaves S.,Cieply K.,Nalesnik M.,and Michalopoulos G.K.,2001,Comparative genomic hybridization of hepatocellular carcinoma:correlation with fluorescence in situ,hybridization in paraffin-embedded tissue,Molecular Diagnosis,6(1):27-37
Tornillo L.,Carafa V.,Sauter G.,Moch H.,Minola E.,Gambacorta M.,Vecchione R.,Bianchi L.,and Terracciano L.M.,2002,Chromosomal alterations in hepatocellular nodules by comparative genomic hybridization:high-grade dysplastic nodules represent early stages of hepatocellular carcinoma,Laboratory Investigation,82(5):547-553
Wan X.X.,Zhu Z.Z.,Wen B.J.,Chen L.,Bao L.L.,He S.Q.,Hu L.Y.,and Cong W.M.,2016,A preliminary study on molecular markers of DNA copy number aberration for extrahepatic metastasis in patients with hepatocellular carcinoma after surgery,Zhonghua Gandan Waike Zazhi(Chinese Journal of Hepatobiliary Surgery),22(8):522-525(万小希,朱忠政,闻炳基,陈律,鲍玲玲,贺松琴,胡柳燕,丛文铭,2016,肝癌患者术后肝外转移相关DNA拷贝数变异分子标志初探,中华肝胆外科杂志,22(8):522-525)
Chen Y.J.,Yeh S.H.,Chen J.T.,Wu C.C.,Hsu M.T.,Tsai S.F.,Chen P.J.,and Lin C.H.,2000,Chromosomal changes and clonality relationship between primary and recurrent hepatocellular carcinoma,Gastroenterology,119(2):431-440
Gebhart E.,2004,Comparative genomic hybridization(CGH):ten years of substantial progress in human solid tumor molecular cytogenetics,Cytogenetic and Genome Research,104(1-4):352-358
He S.Q.,Cong W.M.,Hu L.Y.,Yan J.X.,Dong H.,Chen L.,Min B.J.,and Zhu Z.Z.,2015,Copy number alterations and phenotyp ic genes in chromosome 4 long arm related to recurrence of hepatocellular carcinoma,Zhonghua Shiyan Waike Zazhi(Chinese Journal of Experimental Surgery),32(10):2376-2379(贺松琴,丛文铭,胡柳燕,严静娴,董辉,陈律,闵炳基,朱忠政,2015,4号染色体长臂拷贝数变异及复发表征基因与肝癌复发,中华实验外科杂志,32(10):2376-2379)
Pan Q.,and Ding K.Y.,2015,Droplet digital PCR validates a novel nonsense somatic mutation in MSH2 in hepatic carcinoma,Disan Junyi Daxue Xuebao(Journal of Third Military Medical University),37(23):2368-2372(潘琦,丁克越,2015,微滴数字化PCR检测肝癌基因组MSH2基因的一个新的无义突变,第三军医大学学报,37(23):2368-2372)
Qiu S.,and Zhang H.Y.,2015,Detection of human hepatocarcinoma cell line PLC/PRF/5 genome hepatitis b virus DNA integration with Alu-PCR,Yixue Yanjiu Zazhi(Journal of Me-dical Research),44(4):88-90,41(邱爽,张会英,2015,Alu-PCR检测人肝癌细胞株PLC/PRF/5基因组中乙型肝炎病毒DNA的整合,医学研究杂志,44(4):88-90,41)
Rao U.N.,Gollin S.M.,Beaves S.,Cieply K.,Nalesnik M.,and Michalopoulos G.K.,2001,Comparative genomic hybridization of hepatocellular carcinoma:correlation with fluorescence in situ,hybridization in paraffin-embedded tissue,Molecular Diagnosis,6(1):27-37
Tornillo L.,Carafa V.,Sauter G.,Moch H.,Minola E.,Gambacorta M.,Vecchione R.,Bianchi L.,and Terracciano L.M.,2002,Chromosomal alterations in hepatocellular nodules by comparative genomic hybridization:high-grade dysplastic nodules represent early stages of hepatocellular carcinoma,Laboratory Investigation,82(5):547-553
Wan X.X.,Zhu Z.Z.,Wen B.J.,Chen L.,Bao L.L.,He S.Q.,Hu L.Y.,and Cong W.M.,2016,A preliminary study on molecular markers of DNA copy number aberration for extrahepatic metastasis in patients with hepatocellular carcinoma after surgery,Zhonghua Gandan Waike Zazhi(Chinese Journal of Hepatobiliary Surgery),22(8):522-525(万小希,朱忠政,闻炳基,陈律,鲍玲玲,贺松琴,胡柳燕,丛文铭,2016,肝癌患者术后肝外转移相关DNA拷贝数变异分子标志初探,中华肝胆外科杂志,22(8):522-525)