重庆巫山地区采食玉米的草地贪夜蛾肠道细菌的分离鉴定补遗
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摘要
为充分认识入侵重庆地区草地贪夜蛾(Spodoptera frugiperda)肠道所携带的细菌组成,以采集自巫山地区玉米地的草地贪夜蛾为材料,在课题组前期研究基础上进一步分离培养并通过16S rDNA测序鉴定了3个未报道的分离株,分别归为短波单胞菌属(Brevundimonas)、金黄杆菌属(Chryseobacterium)以及肠球菌属(Enterococcus).结论进一步丰富了入侵重庆巫山地区草地贪夜蛾肠道优势细菌的种类,为后续研究草地贪夜蛾肠道微生物对宿主的生长发育以及迁飞等重要问题奠定了基础.
In order to discover the composition of intestinal bacteria in Spodoptera frugiperda feeding on maize, which migrated to Chongqing in May of 2019, we obtained three unreported isolates in the pest samples collected in maize fields of Wushan by 16 S rDNA sequencing on the basis of the previous research. They were shown to belong to Brevundimonas, Chryseobacterium and Enterococcus respectively. This experiment enriched the biodiversity of intestinal dominant bacteria of the S.frugiperda in Wushan county of Chongqing, which may serve as a basis for the follow-up study on the impact of the intestinal microbes on the growth, development and migration of S.frugiperda.
In order to discover the composition of intestinal bacteria in Spodoptera frugiperda feeding on maize, which migrated to Chongqing in May of 2019, we obtained three unreported isolates in the pest samples collected in maize fields of Wushan by 16 S rDNA sequencing on the basis of the previous research. They were shown to belong to Brevundimonas, Chryseobacterium and Enterococcus respectively. This experiment enriched the biodiversity of intestinal dominant bacteria of the S.frugiperda in Wushan county of Chongqing, which may serve as a basis for the follow-up study on the impact of the intestinal microbes on the growth, development and migration of S.frugiperda.
引文
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[3] 向芸庆,王晓强,冯伟,等.不同饲料饲养家蚕其肠道微生态优势菌群类型的组成及差异性 [J].生态学报,2010,30(14):3875-3882.
[4] CHEN B S,DU K Q,SUN C,et al.Gut Bacterial and Fungal Communities of the Domesticated Silkworm (Bombyx mori) and Wild Mulberry-Feeding Relatives [J].The ISME Journal,2018,12(9):2252-2262.
[5] 陈冬平,陈志廷,徐汉虹,等.不同分子标记对入侵广州草地贪夜蛾的寄主型鉴别能力比较 [J/OL].华南农业大学学报:1-5[2019-06-27].http://kns.cnki.net/kcms/detail/44.1110.S.20190617.1027.002.html.
[6] 秦誉嘉,蓝帅,赵紫华,等.迁飞性害虫草地贪夜蛾在我国的潜在地理分布 [J/OL].植物保护:1-8[2019-07-01].https://doi.org/10.16688/j.zwbh.2019269.
[7] 张磊,靳明辉,张丹丹,等.入侵云南草地贪夜蛾的分子鉴定 [J].植物保护,2019,45(2):19-24,56.
[8] 齐国君,马健,胡高,等.首次入侵广东的草地贪夜蛾迁入路径及天气背景分析 [J/OL].环境昆虫学报:1-13[2019-07-01].http://kns.cnki.net/kcms/detail/44.1640.Q.20190601.1228.004.html.
[9] ALMEIDA L G D,MORAESL A B D,TRIGO J R,et al.The Gut Microbiota of Insecticide-Resistant Insects Houses Insecticide-Degrading Bacteria:A Potential Source for Biotechnological Exploitation [J].PLoS One,2017,12(3):e0174754.
[10] ACEVEDO F E,PEIFFER M,TAN C W,et al.Fall Armyworm-Associated Gut Bacteria Modulate Plant Defense Responses [J].Molecular Plant-Microbe Interactions,2017,30(2):127-137.
[11] 唐运林,吴燕燕,顾偌铖,等.重庆地区草地贪夜蛾肠道细菌的分离鉴定 [J].西南大学学报(自然科学版),2019,41(7):8-14.
[12] TAMURA K,PETERSON D,PETERSON N,et al.MEGA5:Molecular Evolutionary Genetics Analysis Using Maximum Likelihood,Evolutionary Distance,and Maximum Parsimony Methods [J].Molecular Biology and Evolution,2011,28(10):2731-2739.