全波长扫描式多功能读数仪-靛酚蓝比色法测定水样铵态氮含量
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摘要
全波长扫描式多功能读数仪(酶标仪)是一种多通道光学系统的分光光度计,将靛酚蓝比色法与酶标仪相结合,建立了酶标仪-靛酚蓝测定水中铵态氮的方法,方法检出限为0.046 mg/L。方法的加标回收率在90.7%~101.8%之间,该方法与连续流动分析仪-水杨酸分光光度法相比,两者测定数据之间回归直线方程为Y(连续流动分析仪-NH_4~+-N)=1.052 4X(酶标仪-NH_4~+-N)-0.009,相关系数R=0.976 1**(n=32,P<0.01)。5个水样重复6次测定,测定结果的相对标准偏差均小于3%。全波长扫描式多功能读数仪(酶标仪)结合靛酚蓝比色法测定重现性好,结果准确,快速方便,可用于水样中铵态氮含量测定。
The indophenol-blue colorimetric method combined with a microplate reader with standard 96-well microplate format was developed to determine ammonium in water samples. The detection limit was 0. 046 mg/L. Values of recovery were in the range of 90. 7% ~ 101. 8% when adding the standard NH_4~+-N solution to five water samples. The NH_4~+-N values measured by the colorimetric method with microplate reader( X) were correlated with by continuous flow analysis and salicylic acid spectrophotometry( Y). The regression equation was Y = 1. 052 4 X-0. 009. The correlation coefficient was 0. 976 1**(n = 32,P <0. 01) and linear slope was near 1. 0. The relative standard deviation( RSD) of proposed colorimetric method with microplate reader was less than 3%( n = 6). It is suggested that the indophenol blue microcolorimetric assays based on a standard96-well microplate format and microplate reader could be used to simplify the quantification of ammonium in water samples.
The indophenol-blue colorimetric method combined with a microplate reader with standard 96-well microplate format was developed to determine ammonium in water samples. The detection limit was 0. 046 mg/L. Values of recovery were in the range of 90. 7% ~ 101. 8% when adding the standard NH_4~+-N solution to five water samples. The NH_4~+-N values measured by the colorimetric method with microplate reader( X) were correlated with by continuous flow analysis and salicylic acid spectrophotometry( Y). The regression equation was Y = 1. 052 4 X-0. 009. The correlation coefficient was 0. 976 1**(n = 32,P <0. 01) and linear slope was near 1. 0. The relative standard deviation( RSD) of proposed colorimetric method with microplate reader was less than 3%( n = 6). It is suggested that the indophenol blue microcolorimetric assays based on a standard96-well microplate format and microplate reader could be used to simplify the quantification of ammonium in water samples.
引文
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[2]叶育万,陈明,杨立君,等.铵态氮值水杨酸流动注射仪器法与纳氏试剂比色法对比实验研究[J].化工科技与开发,2014,43(1):38-41.
[3]HJ 666-2013,水质氨氮的测定流动注射-水杨酸分光光度法[S].
[4]HJ 665-2013,水质氨氮的测定连续流动-水杨酸分光光度法[S].
[5]Ivancic I,Degobbis D,Degobbis D.An optimal manual procedure for ammonia analysis in natural waters by the indophenol blue method[J].Water Research,1984,18(9):1143-1147.
[6]Hernández-López J,Vargas-Albores F.A microplate technique to quantify nutrients(NO-2,NO-3,NH+4and PO3-4)in seawater[J].Aquaculture Research,2015,34(13):1201-1204.
[7]Shand C A,Williams B L,Coutts G.Determination of N-species in soil extracts using microplate techniques[J].Talanta,2008,74(4):648-654.
[8]Currie L A.Nomenclature in evaluation of analytical methods including detection and quantification capabilities(IUPAC recommendions 1995)[J].Pure and Applied Chemistry,1995,67:1699-1723.
[9]Searle P L.The berthelot or indophenol reaction and its use in the analytical chemistry of nitrogen[J].Analyst,1984,109:549-568.
[10]Sraj L O C,Almeida M I G S,Swearer S E,et al.Analytical challenges and advantages of using flow-based methodologies for ammonia determination in estuarine and marine waters[J].Trac Trends in Analytical Chemistry,2014,59:83-92.
[11]张卫宏,赵萍.海水中氨氮的流动注射光度法分析[J].海洋环境科学,2013,32(2):289-291.
[12]黄晓.靛酚蓝法测定海水、河口水中铵氮的“盐效应”及其消除[J].海峡科学,2013,(7):3-6.